{ "uniprot_canonical_ac": "A0JPP4-1", "ptm_annotation": [ { "annotation": "Glycosylated. In testis there are two predominant forms of 77- and 67-kDa and a form of 47-kDa, whereas in epididymal sperm from caput, corpus, and cauda there are two forms of 47- and 43-kDa. Testis forms contain complex carbohydrate residues. Epididymal sperm forms are N-glycosylated. Then undergoes significant glycosylation in the testis and that the majority of these glycoconjugates are removed by the time sperm reach the caput epididymis.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A0JPP4", "id": "A0JPP4", "database": "UniProtKB" }, { "url": "https://www.ncbi.nlm.nih.gov/pubmed/Q9D5A0", "id": "Q9D5A0", "database": "PubMed" } ] } ], "sequence": { "header": "sp|A0JPP4-1|SPESP_RAT Isoform 1 of Sperm equatorial segment protein 1 OS=Rattus norvegicus OX=10116 GN=Spesp1 PE=2 SV=1 CANONICAL=A0JPP4-1", "length": 409, "sequence": "MKPVVLVALLWLWPSSFLAYPTATVLPDEEQNLNHYVQILQNLIMSVPTKEQGFGRKFRSSRTVDGAEARSLSSEMLLTPGLVSAQEVTTPETEVMLTSMVEKTTFPSSGFTMESEKRRTHSKAFWSIQPNNVSIVLHTEEPYIEKEPEPEPELETEEESRRLATEPEPEPESESAPETETRQTAESEEEVETSTAQNKVRTGTSRMSTVITQTTNTQGTPNTVTVKTINNLDVSTESEDVPQLSGQSEILNVEDLPGHHSLSTRHENILRKISNINSQIHQGLLSDHSSPAYKEFIKASREHLKRSLALAAAAEHKLQQMYGSNVFLDGHTSDPDDDMEVIINMLYNSRSKLSDYFTINHVPSELREKATVVIAVLKKILCVDQVEMQSLIRKLLSNNMKILNILNVP" }, "uniprot_id": "", "glycation": [], "keywords": [ "glycoprotein", "predicted.uniprotkb_gly" ], "species": [ { "common_name": "Rat", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A0JPP4", "id": "A0JPP4", "database": "UniProtKB" } ], "name": "Rattus norvegicus", "taxid": 10116 } ], "reaction_enzymes": [], "publication": [ { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/20375058", "type": "PubMed", "id": "20375058" } ], "title": "Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse.", "journal": "Journal of cell science", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001071150.1", "id": "NP_001071150.1", "database": "RefSeq" } ], "authors": "Fujihara Y, Murakami M, Inoue N, Satouh Y, Kaseda K, Ikawa M, Okabe M", "date": "2010" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/25761597", "type": "PubMed", "id": "25761597" } ], "title": "Dynamic Changes in Equatorial Segment Protein 1 (SPESP1) Glycosylation During Mouse Spermiogenesis.", "journal": "Biology of reproduction", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001071150.1", "id": "NP_001071150.1", "database": "RefSeq" } ], "authors": "Suryavathi V, Panneerdoss S, Wolkowicz MJ, Shetty J, Sherman NE, Flickinger CJ, Herr JC", "date": "2015" } ], "uniprot_ac": "A0JPP4", "gene_names": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/A0JPP4", "type": "synonym", "resource": "RefSeq", "name": "RGD1563468" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/A0JPP4", "type": "synonym", "resource": "RefSeq", "name": "Spesp1" }, { "url": "https://www.uniprot.org/uniprot/A0JPP4", "type": "recommended", "resource": "UniProtKB", "name": "Spesp1" } ], "reactions": [], "isoforms": [ { "url": "https://www.uniprot.org/uniprot/A0JPP4#A0JPP4-1", "isoform_ac": "A0JPP4-1", "locus": { "end_pos": 67217981, "strand": "+", "start_pos": 67232148, "chromosome": "8", "evidence": [ { "url": "https://www.ensembl.org/id/ENSRNOT00000049441", "id": "ENSRNOT00000049441", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSRNOP00000043936", "id": "ENSRNOP00000043936", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "sp|A0JPP4-1|SPESP_RAT Isoform 1 of Sperm equatorial segment protein 1 OS=Rattus norvegicus OX=10116 GN=Spesp1 PE=2 SV=1 CANONICAL=A0JPP4-1", "length": 409, "sequence": "MKPVVLVALLWLWPSSFLAYPTATVLPDEEQNLNHYVQILQNLIMSVPTKEQGFGRKFRSSRTVDGAEARSLSSEMLLTPGLVSAQEVTTPETEVMLTSMVEKTTFPSSGFTMESEKRRTHSKAFWSIQPNNVSIVLHTEEPYIEKEPEPEPELETEEESRRLATEPEPEPESESAPETETRQTAESEEEVETSTAQNKVRTGTSRMSTVITQTTNTQGTPNTVTVKTINNLDVSTESEDVPQLSGQSEILNVEDLPGHHSLSTRHENILRKISNINSQIHQGLLSDHSSPAYKEFIKASREHLKRSLALAAAAEHKLQQMYGSNVFLDGHTSDPDDDMEVIINMLYNSRSKLSDYFTINHVPSELREKATVVIAVLKKILCVDQVEMQSLIRKLLSNNMKILNILNVP" } } ], "pathway": [ { "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+rno:501010", "resource": "KEGG Pathway", "id": "rno:501010", "name": "" } ], "reaction_participants": [], "function": [ { "url": "https://www.uniprot.org/uniprot/A0JPP4#function", "annotation": "Involved in fertilization ability of sperm.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A0JPP4#function", "id": "A0JPP4", "database": "UniProtKB" } ] } ], "orthologs": [ { "uniprot_canonical_ac": "Q6UW49-1", "protein_name": "Sperm equatorial segment protein 1", "sequence": { "length": 350, "sequence": "MKPLVLLVALLLWPSSVPAYPSITVTPDEEQNLNHYIQVLENLVRSVPSGEPGREKKSNSPKHVYSIASKGSKFKELVTHGDASTENDVLTNPISEETTTFPTGGFTPEIGKKKHTESTPFWSIKPNNVSIVLHAEEPYIENEEPEPEPEPAAKQTEAPRMLPVVTESSTSPYVTSYKSPVTTLDKSTGIGISTESEDVPQLSGETAIEKPEEFGKHPESWNNDDILKKILDINSQVQQALLSDTSNPAYREDIEASKDHLKRSLALAAAAEHKLKTMYKSQLLPVGRTSNKIDDIETVINMLCNSRSKLYEYLDIKCVPPEMREKAATVFNTLKNMCRSRRVTALLKVY" }, "evidence": [ { "url": "http://www.informatics.jax.org/homology/49838", "id": "49838", "database": "MGI" }, { "url": "https://omabrowser.org/oma/group/A0JPP4/", "id": "A0JPP4", "database": "OMA" } ], "common_name": "Human", "organism": "Homo sapiens", "gene_name": "SPESP1", "tax_id": 9606 }, { "uniprot_canonical_ac": "Q9D5A0-1", "protein_name": "Sperm equatorial segment protein 1", "sequence": { "length": 399, "sequence": "MKLVVLVALWLWPSSLLAYPTITVLPDEEQNLNHYVHILQNLIMSVPTKEQDLGKKLSSSRTVDSAEPRSLASKVLLTPGLVSAQDVTPESDVLIRPVDETTNSRTRGFTLRRRRTQSTAFWSIRPNNISVVLRTEEPFIEKEPEPELESSRLPTEPEPELEPEPEPVAESRQMSEPEEELVTSTTPNKELTGTSRISSMATQPANTQATRITVTVKTTSTMDVSTDSEDVPQLSGQSEIPSAEDLPGRHSLNTRHEDILKKISNINAEIQQGLLGGNNSPEFKEFIKASREHLKRSLALAAAAEHKLEQMYGSNVFPEGRTSDPDNDMEMIINMLYNSRSKLSDYFNIKRVPSELREKASVVNAELRKILCVDQVEMQSLIKKLLSNNMKILNILNVP" }, "evidence": [ { "url": "http://www.informatics.jax.org/homology/49838", "id": "49838", "database": "MGI" }, { "url": "https://omabrowser.org/oma/group/A0JPP4/", "id": "A0JPP4", "database": "OMA" } ], "common_name": "Mouse", "organism": "Mus musculus", "gene_name": "Spesp1", "tax_id": 10090 } ], "interactions": [], "synthesized_glycans": [], "snv": [], "cluster_types": [ { "name": "homologset.mgi", "label": "MGI Homologs" }, { "name": "homologset.oma", "label": "OMA Homologs" } ], "refseq": { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001071150.1", "ac": "NP_001071150.1", "name": "sperm equatorial segment protein 1 precursor", "summary": "" }, "go_annotation": { "categories": [ { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0001669", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A0JPP4", "id": "A0JPP4", "database": "UniProtKB" } ], "name": "acrosomal vesicle", "id": "GO:0001669" } ], "total": 1, "name": "Cellular Component" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0035036", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A0JPP4", "id": "A0JPP4", "database": "UniProtKB" } ], "name": "sperm-egg recognition", "id": "GO:0035036" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0007275", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A0JPP4", "id": "A0JPP4", "database": "UniProtKB" } ], "name": "multicellular organism development", "id": "GO:0007275" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0007342", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A0JPP4", "id": "A0JPP4", "database": "UniProtKB" } ], "name": "fusion of sperm to egg plasma membrane involved in single fertilization", "id": "GO:0007342" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0009566", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A0JPP4", "id": "A0JPP4", "database": "UniProtKB" } ], "name": "fertilization", "id": "GO:0009566" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0007340", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A0JPP4", "id": "A0JPP4", "database": "UniProtKB" } ], "name": "acrosome reaction", "id": "GO:0007340" } ], "total": 5, "name": "Biological Process" } ] }, "site_annotation": [ { "end_pos": 134, "annotation": "n_glycosylation_sequon", "start_pos": 132 } ], "pro_annotation": [], "crossref": [ { "url": "https://bgee.org/?page=gene&gene_id=ENSRNOG00000025359", "id": "ENSRNOG00000025359", "database": "Bgee" }, { "url": "https://www.ncbi.nlm.nih.gov/gene/501010", "id": "501010", "database": "GeneID" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR026743", "id": "IPR026743", "database": "InterPro" }, { "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+rno:501010", "id": "rno:501010", "database": "KEGG Pathway" }, { "url": "https://omabrowser.org/oma/group/TAFWSIK/", "id": "TAFWSIK", "database": "OMA" }, { "url": "http://www.orthodb.org/?query=1348911at2759", "id": "1348911at2759", "database": "OrthoDB" }, { "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR31667", "id": "PTHR31667", "database": "PANTHER" }, { "url": "http://pfam.xfam.org/family/PF15754", "id": "PF15754", "database": "Pfam" }, { "url": "http://purl.obolibrary.org/obo/PR_PR_A0JPP4", "id": "PR_A0JPP4", "database": "PRO" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001071150", "id": "NP_001071150", "database": "RefSeq" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001071150.1", "id": "NP_001071150.1", "database": "RefSeq" }, { "url": "http://rgd.mcw.edu/tools/genes/genes_view.cgi?id=1563468", "id": "1563468", "database": "RGD" }, { "url": "https://www.uniprot.org/uniprot/A0JPP4", "id": "A0JPP4", "database": "UniProtKB" }, { "url": "https://www.uniprot.org/uniprot/A0JPP4-1", "id": "A0JPP4-1", "database": "UniProtKB" } ], "expression_disease": [], "glycosylation": [ { "comment": "N-linked (GlcNAc...) asparagine", "site_category": "predicted", "end_pos": 132, "residue": "Asn", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A0JPP4#ptm_processing", "id": "A0JPP4", "database": "UniProtKB" } ], "subtype": "", "relation": "attached", "start_pos": 132, "glytoucan_ac": "", "type": "N-linked" } ], "mutagenesis": [], "disease": [], "phosphorylation": [], "mass": { "monoisotopic_mass": 45880.0, "chemical_mass": 45880.0 }, "expression_tissue": [], "gene": [ { "url": "https://www.uniprot.org/uniprot/A0JPP4", "name": "Spesp1", "locus": { "end_pos": 67217802, "strand": "-", "start_pos": 67232298, "chromosome": "8", "evidence": [ { "url": "https://www.ensembl.org/id/ENSRNOG00000025359", "id": "ENSRNOG00000025359", "database": "Ensembl Gene" } ] } } ], "protein_names": [ { "url": "https://www.uniprot.org/uniprot/A0JPP4#names_and_taxonomy", "type": "recommended", "resource": "UniProtKB", "name": "Sperm equatorial segment protein 1" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/A0JPP4", "type": "synonym", "resource": "RefSeq", "name": "sperm equatorial segment protein 1 precursor" } ] }