{ "uniprot_canonical_ac": "A4Q9F0-1", "ptm_annotation": [], "sequence": { "header": "sp|A4Q9F0-1|TTLL7_MOUSE Isoform 1 of Tubulin polyglutamylase TTLL7 OS=Mus musculus OX=10090 GN=Ttll7 PE=1 SV=1 CANONICAL=A4Q9F0-1", "length": 912, "sequence": "MPSLPQDGVIQGSSPVDLGTELPYQCTMKRKVRKKKKKGIITANVAGTKFEIVRLVIDEMGFMKTPDEDETSNLIWCDAAVQQEKITDLQNYQRINHFPGMGEICRKDFLARNMTKMIKSRPMDYTFVPRTWIFPSEYTQFQNYVKELKKKRKQKTFIVKPANGAMGHGISLIRNGDKVPSQDHLIVQEYIEKPFLMEGYKFDLRIYILVTSCDPLKIFLYHDGLVRMGTEKYIPPNESNLTQLYMHLTNYSVNKHNERFERNETEDKGSKRSIKWFTEFLQANQHDVTKFWSDISELVVKTLIVAEPHVLHAYRMCRPGQPPGSESVCFEVLGFDILLDRKLKPWLLEINRAPSFGTDQKIDYDVKRGVLLNALKLLNIRTSDKRKNLAKQKAEAQRRLYGQNPVRRLSPGSSDWEQQRHQLERRKEELKERLLQVRKQVSQEEHENRHMGNYRRIYPPEDKALLEKYEGLLAVAFQTFLSGRAASFQREMNNPLKKMREEDLLDLLEQCEIDDEKLMGKTGRVRGPKPLCCMPECAEVTKKQKYYGSSDSSYDSSSSSSNSELDENEKELCQKRLDQVPYSLKHTSHCKIIQQPSGSHNLIYSESPVYLTTLVFLSEFPDSMRRSVSCPRSISAHLPSRGDVRPFSSQQVIPLARPTSASRSHSLNRASSYARHLPHGSDTGSTNTLNESLRQLKTKEQEDDLTSQTLFVLKDMRIRFPGKSDAESELLIEDIMDNWKHYKTKVASYWLIKLDSVKQRKVLDIVKSSIRTVLPRIWRVPDAEELSLYRIFNRVFNRLLWSHGQGLWSCFCDSGSSWESIFSKSPEVVTPLQLQCCQRLVELCKQCLLVVYKYTTETRGPISGIGPDWGNSRYLLPGSTQFLMRSPLYNMKYNSPGMTRSNVLFTSRYGRL" }, "uniprot_id": "A0A0G2JFF6_MOUSE", "enzyme_annotation": [ { "ec": "6.-.-.-", "activity": "" } ], "glycation": [], "keywords": [ "enzyme" ], "species": [ { "common_name": "Mouse", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" } ], "name": "Mus musculus", "taxid": 10090 } ], "go_annotation": { "categories": [ { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005524", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" } ], "name": "ATP binding", "id": "GO:0005524" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0070740", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" } ], "name": "tubulin-glutamic acid ligase activity", "id": "GO:0070740" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0048487", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" } ], "name": "beta-tubulin binding", "id": "GO:0048487" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0043014", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" } ], "name": "alpha-tubulin binding", "id": "GO:0043014" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0070740", "pmid": "19152315", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" } ], "name": "tubulin-glutamic acid ligase activity", "id": "GO:0070740" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0015631", "pmid": "21873635", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" } ], "name": "tubulin binding", "id": "GO:0015631" } ], "total": 6, "name": "Molecular Function" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005929", "pmid": "21873635", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" } ], "name": "cilium", "id": "GO:0005929" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005737", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" } ], "name": "cytoplasm", "id": "GO:0005737" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005874", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" } ], "name": "microtubule", "id": "GO:0005874" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0030425", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" } ], "name": "dendrite", "id": "GO:0030425" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0043204", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" } ], "name": "perikaryon", "id": "GO:0043204" } ], "total": 5, "name": "Cellular Component" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0006464", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" } ], "name": "cellular protein modification process", "id": "GO:0006464" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0018095", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" } ], "name": "protein polyglutamylation", "id": "GO:0018095" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0000226", "pmid": "21873635", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" } ], "name": "microtubule cytoskeleton organization", "id": "GO:0000226" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0018095", "pmid": "19152315", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" } ], "name": "protein polyglutamylation", "id": "GO:0018095" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0007399", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" } ], "name": "nervous system development", "id": "GO:0007399" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0030154", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" } ], "name": "cell differentiation", "id": "GO:0030154" } ], "total": 6, "name": "Biological Process" } ] }, "publication": [ { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/16901895", "type": "PubMed", "id": "16901895" } ], "title": "TTLL7 is a mammalian beta-tubulin polyglutamylase required for growth of MAP2-positive neurites.", "journal": "J. Biol. Chem.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289887.1", "id": "NP_001289887.1", "database": "RefSeq" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289886.1", "id": "NP_001289886.1", "database": "RefSeq" } ], "authors": "Setou M., Ikegami K., Mukai M., Tsuchida J., Heier R.L., Macgregor G.R.", "date": "2006" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/17360631", "type": "PubMed", "id": "17360631" } ], "title": "Loss of alpha-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function.", "journal": "Proceedings of the National Academy of Sciences of the United States of America", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289886.1", "id": "NP_001289886.1", "database": "RefSeq" } ], "authors": "Ikegami K, Heier RL, Taruishi M, Takagi H, Mukai M, Shimma S, Taira S, Hatanaka K, Morone N, Yao I, Campbell PK, Yuasa S, Janke C, Macgregor GR, Setou M", "date": "2007" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/17499049", "type": "PubMed", "id": "17499049" } ], "title": "A targeted multienzyme mechanism for selective microtubule polyglutamylation.", "journal": "Mol. Cell", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289886.1", "id": "NP_001289886.1", "database": "RefSeq" } ], "authors": "Janke C., Lacroix B., Rogowski K., van Dijk J., Miro J., Edde B.", "date": "2007" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/19152315", "type": "PubMed", "id": "19152315" } ], "title": "Recombinant mammalian tubulin polyglutamylase TTLL7 performs both initiation and elongation of polyglutamylation on beta-tubulin through a random sequential pathway.", "journal": "Biochemistry", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289887.1", "id": "NP_001289887.1", "database": "RefSeq" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289886.1", "id": "NP_001289886.1", "database": "RefSeq" } ], "authors": "Nakagawa A., Takeshita K., Setou M., Ikegami K., Mukai M., Sugiura Y.", "date": "2009" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/25959773", "type": "PubMed", "id": "25959773" } ], "title": "Multivalent Microtubule Recognition by Tubulin Tyrosine Ligase-like Family Glutamylases.", "journal": "Cell", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289886.1", "id": "NP_001289886.1", "database": "RefSeq" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289887.1", "id": "NP_001289887.1", "database": "RefSeq" } ], "authors": "Garnham CP, Vemu A, Wilson-Kubalek EM, Yu I, Szyk A, Lander GC, Milligan RA, Roll-Mecak A", "date": "2015" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/29593216", "type": "PubMed", "id": "29593216" } ], "title": "Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.", "journal": "Nature communications", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289886.1", "id": "NP_001289886.1", "database": "RefSeq" } ], "authors": "Ye B, Liu B, Hao L, Zhu X, Yang L, Wang S, Xia P, Du Y, Meng S, Huang G, Qin X, Wang Y, Yan X, Li C, Hao J, Zhu P, He L, Tian Y, Fan Z", "date": "2018" } ], "uniprot_ac": "A4Q9F0", "gene_names": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/A4Q9F0", "type": "synonym", "resource": "RefSeq", "name": "1110049N09Rik; 4921517B04Rik; C630030B20; mTTLL7" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/A4Q9F0", "type": "synonym", "resource": "RefSeq", "name": "Ttll7" }, { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "type": "recommended", "resource": "UniProtKB", "name": "Ttll7" } ], "reactions": [ { "source": "Reactome", "output_participant_list": [], "name": "TTLLs polyglutamylate tubulin", "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-8865774", "cellular_location": "plasma membrane", "input_participant_list": [], "enzyme_list": [ "A4Q9E4-1", "Q80UG8-1", "A2APC3-1", "A4Q9E8-1", "A4Q9F0-1", "Q8CHB8-1", "A4Q9F4-1", "A4Q9F6-1" ], "id": "R-MMU-8865774", "summary": "This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp" } ], "isoforms": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0#A0A0G2JFF6-1", "isoform_ac": "A0A0G2JFF6-1", "locus": { "end_pos": 146896656, "strand": "-", "start_pos": 146897815, "chromosome": "3", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000124743", "id": "ENSMUST00000124743", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000143151", "id": "ENSMUSP00000143151", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|A0A0G2JFF6-1|TTLL7_MOUSE Computationally mapped isoform of Tubulin polyglutamylase TTLL7 OS=Mus musculus OX=10090 GN=Ttll7 PE=1 SV=1 CANONICAL=A4Q9F0-1", "length": 47, "sequence": "ETSNLIWCDAAVQQEKITDLQNYQRINHFPGMGEICRKDFLARNMTK" } }, { "url": "https://www.uniprot.org/uniprot/A4Q9F0#A0A0G2LB90-1", "isoform_ac": "A0A0G2LB90-1", "locus": { "end_pos": 146894176, "strand": "-", "start_pos": 146979748, "chromosome": "3", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000037942", "id": "ENSMUST00000037942", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000043753", "id": "ENSMUSP00000043753", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|A0A0G2LB90-1|TTLL7_MOUSE Computationally mapped isoform of Tubulin polyglutamylase TTLL7 OS=Mus musculus OX=10090 GN=Ttll7 PE=1 SV=1 CANONICAL=A4Q9F0-1", "length": 912, "sequence": "MPSLPQDGVIQGSSPVDLGTELPYQCTMKRKVRKKKKKGIITANVAGTKFEIVRLVIDEMGFMKTPDEDETSNLIWCDAAVQQEKITDLQNYQRINHFPGMGEICRKDFLARNMTKMIKSRPMDYTFVPRTWIFPSEYTQFQNYVKELKKKRKQKTFIVKPANGAMGHGISLIRNGDKVPSQDHLIVQEYIEKPFLMEGYKFDLRIYILVTSCDPLKIFLYHDGLVRMGTEKYIPPNESNLTQLYMHLTNYSVNKHNERFERNETEDKGSKRSIKWFTEFLQANQHDVTKFWSDISELVVKTLIVAEPHVLHAYRMCRPGQPPGSESVCFEVLGFDILLDRKLKPWLLEINRAPSFGTDQKIDYDVKRGVLLNALKLLNIRTSDKRKNLAKQKAEAQRRLYGQNPVRRLSPGSSDWEQQRHQLERRKEELKERLLQVRKQVSQEEHENRHMGNYRRIYPPEDKALLEKYEGLLAVAFQTFLSGRAASFQREMNNPLKKMREEDLLDLLEQCEIDDEKLMGKTGRVRGPKPLCCMPECAEVTKKQKYYGSSDSSYDSSSSSSNSELDENEKELCQKRLDQVPYSLKHTSHCKIIQQSSGSHNLIYSESPVYLTTLVFLSEFPDSMRRSVSCPRSISAHLPSRGDVRPFSSQQVIPLARPTSASRSHSLNRASSYARHLPHGSDTGSTNTLNESLRQLKTKEQEDDLTSQTLFVLKDMRIRFPGKSDAESELLIEDIMDNWKHYKTKVASYWLIKLDSVKQRKVLDIVKSSIRTVLPRIWRVPDAEELSLYRIFNRVFNRLLWSHGQGLWSCFCDSGSSWESIFSKSPEVVTPLQLQCCQRLVELCKQCLLVVYKYTTETRGPISGIGPDWGNSRYLLPGSTQFLMRSPLYNMKYNSPGMTRSNVLFTSRYGRL" } }, { "url": "https://www.uniprot.org/uniprot/A4Q9F0#A4Q9F0-1", "isoform_ac": "A4Q9F0-1", "locus": {}, "sequence": { "header": "sp|A4Q9F0-1|TTLL7_MOUSE Isoform 1 of Tubulin polyglutamylase TTLL7 OS=Mus musculus OX=10090 GN=Ttll7 PE=1 SV=1 CANONICAL=A4Q9F0-1", "length": 912, "sequence": "MPSLPQDGVIQGSSPVDLGTELPYQCTMKRKVRKKKKKGIITANVAGTKFEIVRLVIDEMGFMKTPDEDETSNLIWCDAAVQQEKITDLQNYQRINHFPGMGEICRKDFLARNMTKMIKSRPMDYTFVPRTWIFPSEYTQFQNYVKELKKKRKQKTFIVKPANGAMGHGISLIRNGDKVPSQDHLIVQEYIEKPFLMEGYKFDLRIYILVTSCDPLKIFLYHDGLVRMGTEKYIPPNESNLTQLYMHLTNYSVNKHNERFERNETEDKGSKRSIKWFTEFLQANQHDVTKFWSDISELVVKTLIVAEPHVLHAYRMCRPGQPPGSESVCFEVLGFDILLDRKLKPWLLEINRAPSFGTDQKIDYDVKRGVLLNALKLLNIRTSDKRKNLAKQKAEAQRRLYGQNPVRRLSPGSSDWEQQRHQLERRKEELKERLLQVRKQVSQEEHENRHMGNYRRIYPPEDKALLEKYEGLLAVAFQTFLSGRAASFQREMNNPLKKMREEDLLDLLEQCEIDDEKLMGKTGRVRGPKPLCCMPECAEVTKKQKYYGSSDSSYDSSSSSSNSELDENEKELCQKRLDQVPYSLKHTSHCKIIQQPSGSHNLIYSESPVYLTTLVFLSEFPDSMRRSVSCPRSISAHLPSRGDVRPFSSQQVIPLARPTSASRSHSLNRASSYARHLPHGSDTGSTNTLNESLRQLKTKEQEDDLTSQTLFVLKDMRIRFPGKSDAESELLIEDIMDNWKHYKTKVASYWLIKLDSVKQRKVLDIVKSSIRTVLPRIWRVPDAEELSLYRIFNRVFNRLLWSHGQGLWSCFCDSGSSWESIFSKSPEVVTPLQLQCCQRLVELCKQCLLVVYKYTTETRGPISGIGPDWGNSRYLLPGSTQFLMRSPLYNMKYNSPGMTRSNVLFTSRYGRL" } }, { "url": "https://www.uniprot.org/uniprot/A4Q9F0#A4Q9F0-2", "isoform_ac": "A4Q9F0-2", "locus": {}, "sequence": { "header": "sp|A4Q9F0-2|TTLL7_MOUSE Isoform 2 of Tubulin polyglutamylase TTLL7 OS=Mus musculus OX=10090 GN=Ttll7 PE=1 SV=1 CANONICAL=A4Q9F0-1", "length": 609, "sequence": "MPSLPQDGVIQGSSPVDLGTELPYQCTMKRKVRKKKKKGIITANVAGTKFEIVRLVIDEMGFMKTPDEDETSNLIWCDAAVQQEKITDLQNYQRINHFPGMGEICRKDFLARNMTKMIKSRPMDYTFVPRTWIFPSEYTQFQNYVKELKKKRKQKTFIVKPANGAMGHGISLIRNGDKVPSQDHLIVQEYIEKPFLMEGYKFDLRIYILVTSCDPLKIFLYHDGLVRMGTEKYIPPNESNLTQLYMHLTNYSVNKHNERFERNETEDKGSKRSIKWFTEFLQANQHDVTKFWSDISELVVKTLIVAEPHVLHAYRMCRPGQPPGSESVCFEVLGFDILLDRKLKPWLLEINRAPSFGTDQKIDYDVKRGVLLNALKLLNIRTSDKRKNLAKQKAEAQRRLYGQNPVRRLSPGSSDWEQQRHQLERRKEELKERLLQVRKQVSQEEHENRHMGNYRRIYPPEDKALLEKYEGLLAVAFQTFLSGRAASFQREMNNPLKKMREEDLLDLLEQCEIDDEKLMGKTGRVRGPKPLCCMPECAEVTKKQKYYGSSDSSYDSSSSSSNSELDENEKELCQKRLDQVPYSLKHTSHCKIIQQSSKSISPTSLEEEL" } }, { "url": "https://www.uniprot.org/uniprot/A4Q9F0#E9PWB8-1", "isoform_ac": "E9PWB8-1", "locus": { "end_pos": 146894176, "strand": "-", "start_pos": 146979748, "chromosome": "3", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000170055", "id": "ENSMUST00000170055", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000129369", "id": "ENSMUSP00000129369", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|E9PWB8-1|E9PWB8_MOUSE Computationally mapped isoform of Tubulin polyglutamylase TTLL7 OS=Mus musculus OX=10090 GN=Ttll7 PE=1 SV=1 CANONICAL=A4Q9F0-1", "length": 892, "sequence": "MPSLPQDGVIQGSSPVDLGTELPYQCTMKRKVRKKKKKGIITANVAGTKFEIVRLVIDEMGFMKTPDEDETSNLIWCDAAVQQEKITDLQNYQRINHFPGMGEICRKDFLARNMTKMIKSRPMDYTFVPRTWIFPSEYTQFQNYVKELKKKRKQKTFIVKPANGAMGHGISLIRNGDKVPSQDHLIVQEYIEKPFLMEGYKFDLRIYILVTSCDPLKIFLYHDGLVRMGTEKYIPPNESNLTQLYMHLTNYSVNKHNERFERNETEDKGSKRSIKWFTEFLQANQHDVTKFWSDISELVVKTLIVAEPHVLHAYRMCRPGQPPGSESVCFEVLGFDILLDRKLKPWLLEINRAPSFGTDQKIDYDVKRGVLLNALKLLNIRTSDKRKNLAKQKAEAQRRLYGQNPVRRLSPGSSDWEQQRHQLERRKEELKERLLQVRKQVSQEEHENRHMGNYRRIYPPEDKALLEKYEGLLAVAFQTFLSGRAASFQREMNNPLKKMREEDLLDLLEQCEIDDEKLMGKTGRVRGPKPLCCMPECAEVTKKQKYYGSSDSSYDSSSSSSNSELDENEKELCQKRLDQVPYSLKHTSHCKIIQQSNSMRRSVSCPRSISAHLPSRGDVRPFSSQQVIPLARPTSASRSHSLNRASSYARHLPHGSDTGSTNTLVSYQWNESLRQLKTKEQEDDLTSQTLFVLKDMRIRFPGKSDAESELLIEDIMDNWKHYKTKVASYWLIKLDSVKQRKVLDIVKSSIRTVLPRIWRVPDAEELSLYRIFNRVFNRLLWSHGQGLWSCFCDSGSSWESIFSKSPEVVTPLQLQCCQRLVELCKQCLLVVYKYTTETRGPISGIGPDWGNSRYLLPGSTQFLMRSPLYNMKYNSPGMTRSNVLFTSRYGRL" } }, { "url": "https://www.uniprot.org/uniprot/A4Q9F0#E9Q6C6-1", "isoform_ac": "E9Q6C6-1", "locus": { "end_pos": 146894176, "strand": "-", "start_pos": 146979748, "chromosome": "3", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000106134", "id": "ENSMUST00000106134", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000101740", "id": "ENSMUSP00000101740", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|E9Q6C6-1|TTLL7_MOUSE Computationally mapped isoform of Tubulin polyglutamylase TTLL7 OS=Mus musculus OX=10090 GN=Ttll7 PE=1 SV=1 CANONICAL=A4Q9F0-1", "length": 887, "sequence": "MPSLPQDGVIQGSSPVDLGTELPYQCTMKRKVRKKKKKGIITANVAGTKFEIVRLVIDEMGFMKTPDEDETSNLIWCDAAVQQEKITDLQNYQRINHFPGMGEICRKDFLARNMTKMIKSRPMDYTFVPRTWIFPSEYTQFQNYVKELKKKRKQKTFIVKPANGAMGHGISLIRNGDKVPSQDHLIVQEYIEKPFLMEGYKFDLRIYILVTSCDPLKIFLYHDGLVRMGTEKYIPPNESNLTQLYMHLTNYSVNKHNERFERNETEDKGSKRSIKWFTEFLQANQHDVTKFWSDISELVVKTLIVAEPHVLHAYRMCRPGQPPGSESVCFEVLGFDILLDRKLKPWLLEINRAPSFGTDQKIDYDVKRGVLLNALKLLNIRTSDKRKNLAKQKAEAQRRLYGQNPVRRLSPGSSDWEQQRHQLERRKEELKERLLQVRKQVSQEEHENRHMGNYRRIYPPEDKALLEKYEGLLAVAFQTFLSGRAASFQREMNNPLKKMREEDLLDLLEQCEIDDEKLMGKTGRVRGPKPLCCMPECAEVTKKQKYYGSSDSSYDSSSSSSNSELDENEKELCQKRLDQVPYSLKHTSHCKIIQQSNSMRRSVSCPRSISAHLPSRGDVRPFSSQQVIPLARPTSASRSHSLNRASSYARHLPHGSDTGSTNTLNESLRQLKTKEQEDDLTSQTLFVLKDMRIRFPGKSDAESELLIEDIMDNWKHYKTKVASYWLIKLDSVKQRKVLDIVKSSIRTVLPRIWRVPDAEELSLYRIFNRVFNRLLWSHGQGLWSCFCDSGSSWESIFSKSPEVVTPLQLQCCQRLVELCKQCLLVVYKYTTETRGPISGIGPDWGNSRYLLPGSTQFLMRSPLYNMKYNSPGMTRSNVLFTSRYGRL" } } ], "pathway": [ { "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:70892", "resource": "KEGG Pathway", "id": "mmu:70892", "name": "" }, { "reaction_list": [ "R-MMU-8865774", "R-MMU-8867370" ], "resource": "Reactome", "description": "Carboxyterminal post-translational modifications of tubulin", "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-8955332", "id": "R-MMU-8955332", "name": "Carboxyterminal post-translational modifications of tubulin" } ], "reaction_participants": [], "function": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0#function", "annotation": "Polyglutamylase which preferentially modifies beta-tubulin (PubMed:16901895, PubMed:17499049, PubMed:19152315). Mediates both ATP-dependent initiation and elongation of polyglutamylation of microtubules (PubMed:16901895, PubMed:19152315). Required for neurite growth; responsible for the strong increase in tubulin polyglutamylation during postnatal neuronal maturation (PubMed:16901895).", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0#function", "id": "A4Q9F0", "database": "UniProtKB" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289886.1", "annotation": "TTLL7 is a mammalian beta-tubulin polyglutamylase required for growth of MAP2-positive neurites", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289886.1", "id": "NP_001289886.1", "database": "RefSeq" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289887.1", "annotation": "TTLL7 is a mammalian beta-tubulin polyglutamylase required for growth of MAP2-positive neurites", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289887.1", "id": "NP_001289887.1", "database": "RefSeq" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289886.1", "annotation": "TTLL7 performs both the initiation and elongation of polyglutamylation on beta-tubulin; the mechanism of binding of three substrates glutamate, ATP, and tubulin to the enzyme is a random sequential pathway.", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289886.1", "id": "NP_001289886.1", "database": "RefSeq" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289887.1", "annotation": "TTLL7 performs both the initiation and elongation of polyglutamylation on beta-tubulin; the mechanism of binding of three substrates glutamate, ATP, and tubulin to the enzyme is a random sequential pathway.", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289887.1", "id": "NP_001289887.1", "database": "RefSeq" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289886.1", "annotation": "TTLL7, the most abundant neuronal glutamylase, adds glutamates preferentially to the beta-tubulin tail; coupled with ensemble and single-molecule biochemistry, the hybrid X-ray and cryo-electron microscopy structure of TTLL7 bound to the microtubule delineates a tripartite microtubule recognition strategy.", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289886.1", "id": "NP_001289886.1", "database": "RefSeq" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289887.1", "annotation": "TTLL7, the most abundant neuronal glutamylase, adds glutamates preferentially to the beta-tubulin tail; coupled with ensemble and single-molecule biochemistry, the hybrid X-ray and cryo-electron microscopy structure of TTLL7 bound to the microtubule delineates a tripartite microtubule recognition strategy.", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289887.1", "id": "NP_001289887.1", "database": "RefSeq" } ] } ], "orthologs": [ { "uniprot_canonical_ac": "Q6ZT98-1", "protein_name": "Tubulin polyglutamylase TTLL7", "sequence": { "length": 887, "sequence": "MPSLPQEGVIQGPSPLDLNTELPYQSTMKRKVRKKKKKGTITANVAGTKFEIVRLVIDEMGFMKTPDEDETSNLIWCDSAVQQEKISELQNYQRINHFPGMGEICRKDFLARNMTKMIKSRPLDYTFVPRTWIFPAEYTQFQNYVKELKKKRKQKTFIVKPANGAMGHGISLIRNGDKLPSQDHLIVQEYIEKPFLMEGYKFDLRIYILVTSCDPLKIFLYHDGLVRMGTEKYIPPNESNLTQLYMHLTNYSVNKHNEHFERDETENKGSKRSIKWFTEFLQANQHDVAKFWSDISELVVKTLIVAEPHVLHAYRMCRPGQPPGSESVCFEVLGFDILLDRKLKPWLLEINRAPSFGTDQKIDYDVKRGVLLNALKLLNIRTSDKRRNLAKQKAEAQRRLYGQNSIKRLLPGSSDWEQQRHQLERRKEELKERLAQVRKQISREEHENRHMGNYRRIYPPEDKALLEKYENLLAVAFQTFLSGRAASFQRELNNPLKRMKEEDILDLLEQCEIDDEKLMGKTTKTRGPKPLCSMPESTEIMKRPKYCSSDSSYDSSSSSSESDENEKEEYQNKKREKQVTYNLKPSNHYKLIQQPSSIRRSVSCPRSISAQSPSSGDTRPFSAQQMISVSRPTSASRSHSLNRASSYMRHLPHSNDACSTNSQVSESLRQLKTKEQEDDLTSQTLFVLKDMKIRFPGKSDAESELLIEDIIDNWKYHKTKVASYWLIKLDSVKQRKVLDIVKTSIRTVLPRIWKVPDVEEVNLYRIFNRVFNRLLWSRGQGLWNCFCDSGSSWESIFNKSPEVVTPLQLQCCQRLVELCKQCLLVVYKYATDKRGSLSGIGPDWGNSRYLLPGSTQFFLRTPTYNLKYNSPGMTRSNVLFTSRYGHL" }, "evidence": [ { "url": "http://www.informatics.jax.org/homology/41578", "id": "41578", "database": "MGI" } ], "common_name": "Human", "organism": "Homo sapiens", "gene_name": "TTLL7", "tax_id": 9606 } ], "interactions": [], "synthesized_glycans": [], "snv": [], "cluster_types": [ { "name": "isoformset.uniprotkb", "label": "Isoforms" }, { "name": "homologset.mgi", "label": "MGI Homologs" } ], "refseq": { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289886.1", "ac": "NP_001289886.1", "name": "tubulin polyglutamylase TTLL7 isoform Ttll7", "summary": "" }, "reaction_enzymes": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "activity": "", "gene_name": "Ttll9", "uniprot_ac": "A2APC3", "ec_number": "6.-.-.-" }, { "url": "https://www.uniprot.org/uniprot/A4Q9E4", "activity": "", "gene_name": "Ttll2", "uniprot_ac": "A4Q9E4", "ec_number": "6.-.-.-" }, { "url": "https://www.uniprot.org/uniprot/A4Q9E8", "activity": "", "gene_name": "Ttll6", "uniprot_ac": "A4Q9E8", "ec_number": "6.-.-.-" }, { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "activity": "", "gene_name": "Ttll7", "uniprot_ac": "A4Q9F0", "ec_number": "6.-.-.-" }, { "url": "https://www.uniprot.org/uniprot/A4Q9F4", "activity": "", "gene_name": "Ttll11", "uniprot_ac": "A4Q9F4", "ec_number": "6.-.-.-" }, { "url": "https://www.uniprot.org/uniprot/A4Q9F6", "activity": "", "gene_name": "Ttll13", "uniprot_ac": "A4Q9F6", "ec_number": "6.-.-.-" }, { "url": "https://www.uniprot.org/uniprot/Q80UG8", "activity": "", "gene_name": "Ttll4", "uniprot_ac": "Q80UG8", "ec_number": "6.-.-.-" }, { "url": "https://www.uniprot.org/uniprot/Q8CHB8", "activity": "", "gene_name": "Ttll5", "uniprot_ac": "Q8CHB8", "ec_number": "6.-.-.-" } ], "site_annotation": [ { "end_pos": 115, "annotation": "n_glycosylation_sequon", "start_pos": 113 }, { "end_pos": 239, "annotation": "n_glycosylation_sequon", "start_pos": 237 }, { "end_pos": 242, "annotation": "n_glycosylation_sequon", "start_pos": 240 }, { "end_pos": 252, "annotation": "n_glycosylation_sequon", "start_pos": 250 }, { "end_pos": 265, "annotation": "n_glycosylation_sequon", "start_pos": 263 }, { "end_pos": 692, "annotation": "n_glycosylation_sequon", "start_pos": 690 } ], "pro_annotation": [], "crossref": [ { "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000036745", "id": "ENSMUSG00000036745", "database": "Bgee" }, { "url": "https://www.ebi.ac.uk/chembldb/target/inspect/CHEMBL2401604", "id": "CHEMBL2401604", "database": "ChEMBL" }, { "url": "https://www.ncbi.nlm.nih.gov/gene/70892", "id": "70892", "database": "GeneID" }, { "url": "https://www.ebi.ac.uk/intact/interactors/id:A4Q9F0*", "id": "A4Q9F0", "database": "IntAct" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR004344", "id": "IPR004344", "database": "InterPro" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR013815", "id": "IPR013815", "database": "InterPro" }, { "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:70892", "id": "mmu:70892", "database": "KEGG Pathway" }, { "url": "http://www.informatics.jax.org/marker/MGI:1918142", "id": "1918142", "database": "MGI" }, { "url": "https://omabrowser.org/oma/group/DVSKFWN/", "id": "DVSKFWN", "database": "OMA" }, { "url": "http://www.orthodb.org/?query=1251554at2759", "id": "1251554at2759", "database": "OrthoDB" }, { "url": "http://pfam.xfam.org/family/PF03133", "id": "PF03133", "database": "Pfam" }, { "url": "http://purl.obolibrary.org/obo/PR_PR_A4Q9F0", "id": "PR_A4Q9F0", "database": "PRO" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-8955332", "id": "R-MMU-8955332", "database": "Reactome" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289886", "id": "NP_001289886", "database": "RefSeq" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289886.1", "id": "NP_001289886.1", "database": "RefSeq" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289887", "id": "NP_001289887", "database": "RefSeq" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001289887.1", "id": "NP_001289887.1", "database": "RefSeq" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/XP_006502151", "id": "XP_006502151", "database": "RefSeq" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/XP_006502151.1", "id": "XP_006502151.1", "database": "RefSeq" }, { "url": "https://www.uniprot.org/uniprot/A0A0G2JFF6", "id": "A0A0G2JFF6", "database": "UniProtKB" }, { "url": "https://www.uniprot.org/uniprot/A0A0G2JFF6-1", "id": "A0A0G2JFF6-1", "database": "UniProtKB" }, { "url": "https://www.uniprot.org/uniprot/A0A0G2LB90", "id": "A0A0G2LB90", "database": "UniProtKB" }, { "url": "https://www.uniprot.org/uniprot/A0A0G2LB90-1", "id": "A0A0G2LB90-1", "database": "UniProtKB" }, { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" }, { "url": "https://www.uniprot.org/uniprot/A4Q9F0-1", "id": "A4Q9F0-1", "database": "UniProtKB" }, { "url": "https://www.uniprot.org/uniprot/A4Q9F0-2", "id": "A4Q9F0-2", "database": "UniProtKB" }, { "url": "https://www.uniprot.org/uniprot/E9PWB8", "id": "E9PWB8", "database": "UniProtKB" }, { "url": "https://www.uniprot.org/uniprot/E9PWB8-1", "id": "E9PWB8-1", "database": "UniProtKB" }, { "url": "https://www.uniprot.org/uniprot/E9Q6C6", "id": "E9Q6C6", "database": "UniProtKB" }, { "url": "https://www.uniprot.org/uniprot/E9Q6C6-1", "id": "E9Q6C6-1", "database": "UniProtKB" } ], "expression_disease": [], "glycosylation": [], "mutagenesis": [ { "sequence_org": "E", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "id": "A4Q9F0", "database": "UniProtKB" }, { "url": "https://www.ncbi.nlm.nih.gov/pubmed/16901895", "id": "16901895", "database": "PubMed" } ], "sequence_mut": "V", "start_pos": 349, "end_pos": 349, "annotation": "Loss of activity." } ], "disease": [], "phosphorylation": [], "mass": { "monoisotopic_mass": 105501.0, "chemical_mass": 105501.0 }, "expression_tissue": [], "gene": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0", "name": "Ttll7", "locus": { "end_pos": 146984009, "strand": "+", "start_pos": 146852367, "chromosome": "3", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUSG00000036745", "id": "ENSMUSG00000036745", "database": "Ensembl Gene" } ] } } ], "protein_names": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F0#names_and_taxonomy", "type": "synonym", "resource": "UniProtKB", "name": "6.-.-.-" }, { "url": "https://www.uniprot.org/uniprot/A4Q9F0#names_and_taxonomy", "type": "synonym", "resource": "UniProtKB", "name": "Tubulin polyglutamylase TTLL7" }, { "url": "https://www.uniprot.org/uniprot/A4Q9F0#names_and_taxonomy", "type": "synonym", "resource": "UniProtKB", "name": "Tubulin--tyrosine ligase-like protein 7" }, { "url": "https://www.uniprot.org/uniprot/A4Q9F0#names_and_taxonomy", "type": "synonym", "resource": "UniProtKB", "name": "mTTLL7" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/A4Q9F0", "type": "synonym", "resource": "RefSeq", "name": "polyglutamylase; tubulin--tyrosine ligase-like protein 7; tubulin polyglutamylase TTLL7" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/A4Q9F0", "type": "synonym", "resource": "RefSeq", "name": "tubulin polyglutamylase TTLL7 isoform Ttll7s" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/A4Q9F0", "type": "synonym", "resource": "RefSeq", "name": "tubulin polyglutamylase TTLL7 isoform Ttll7" } ] }