{ "uniprot_canonical_ac": "A2AVJ5-1", "ptm_annotation": [ { "annotation": "Ubiquitinated. Ubiquitination by RFFL promotes proteasomal degradation of PRR5L thereby modifying the substrate-specific activity of the mTORC2 complex. Ubiquitination by RFFL is stimulated by LPA/lysophosphatidic acid (By similarity).", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "id": "A2AVJ5", "database": "UniProtKB" }, { "url": "https://www.ncbi.nlm.nih.gov/pubmed/", "id": "", "database": "PubMed" } ] } ], "sequence": { "header": "sp|A2AVJ5-1|PRR5L_MOUSE Isoform 1 of Proline-rich protein 5-like OS=Mus musculus OX=10090 GN=Prr5l PE=1 SV=1 CANONICAL=A2AVJ5-1", "length": 370, "sequence": "MTRGLAPLLPIEFHKMGSFRRPRPRFMSSPVLSELPRFQAARQALQLSSNSAWNSVQTAVINVFKGGGLQSNELYALNESIRRLLKSELGSFITDYFQNQLLAKGLSFVEEKIKLCEGDNRIEVLAEVWDHFFTETLPTLQAIFYPVQGQELTIRQISLLGFRDLVLLKVKLGDVLLLAQSKLPSSVIQMLLILQSVHEPTGPSEGYLQLEELVKQVVSPFLSISGDRSCSGPTYSLARRHSRVRPKVTVLNYASLMTTVGRPLNEMVLTPLTEQEGEAYLEKCGSVRRHTVANAHSDIQLLAMATMMHSGLGEEAGGEDKHLLLPPSFPPPHRQCSSEPSILDSPDELELEDVASGSQEDSELNCASLS" }, "uniprot_id": "PRR5L_MOUSE", "enzyme_annotation": [], "glycation": [], "keywords": [], "species": [ { "common_name": "Mouse", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "id": "A2AVJ5", "database": "UniProtKB" } ], "name": "Mus musculus", "taxid": 10090 } ], "publication": [ { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/16829211", "type": [ "PubMed" ], "id": "16829211" } ], "title": "Detection of novel skeletogenesis target genes by comprehensive analysis of a Runx2(-/-) mouse model.", "journal": "Gene expression patterns : GEP", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077279.1", "id": "NP_001077279.1", "database": "RefSeq" } ], "authors": "Hecht J, Seitz V, Urban M, Wagner F, Robinson PN, Stiege A, Dieterich C, Kornak U, Wilkening U, Brieske N, Zwingman C, Kidess A, Stricker S, Mundlos S", "date": "2007" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/22609986", "type": [ "PubMed" ], "id": "22609986" } ], "title": "PRR5L degradation promotes mTORC2-mediated PKC-delta phosphorylation and cell migration downstream of Galpha12.", "journal": "Nat. Cell Biol.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "id": "A2AVJ5", "database": "UniProtKB" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077279.1", "id": "NP_001077279.1", "database": "RefSeq" } ], "authors": "Simon M.I., Wang C., Wang J., Wu D., Gan X., Kozasa T., Alessi D., Offermanns S., Sommer E., Srinivasula S.", "date": "2012" } ], "uniprot_ac": "A2AVJ5", "site_annotation": [ { "end_pos": 80, "annotation": "n_glycosylation_sequon", "start_pos": 78 } ], "isoforms": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5#A2AVJ5-1", "isoform_ac": "A2AVJ5-1", "locus": { "end_pos": 101717069, "strand": "+", "start_pos": 101772293, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000163762", "id": "ENSMUST00000163762", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000127530", "id": "ENSMUSP00000127530", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "sp|A2AVJ5-1|PRR5L_MOUSE Isoform 1 of Proline-rich protein 5-like OS=Mus musculus OX=10090 GN=Prr5l PE=1 SV=1 CANONICAL=A2AVJ5-1", "length": 370, "sequence": "MTRGLAPLLPIEFHKMGSFRRPRPRFMSSPVLSELPRFQAARQALQLSSNSAWNSVQTAVINVFKGGGLQSNELYALNESIRRLLKSELGSFITDYFQNQLLAKGLSFVEEKIKLCEGDNRIEVLAEVWDHFFTETLPTLQAIFYPVQGQELTIRQISLLGFRDLVLLKVKLGDVLLLAQSKLPSSVIQMLLILQSVHEPTGPSEGYLQLEELVKQVVSPFLSISGDRSCSGPTYSLARRHSRVRPKVTVLNYASLMTTVGRPLNEMVLTPLTEQEGEAYLEKCGSVRRHTVANAHSDIQLLAMATMMHSGLGEEAGGEDKHLLLPPSFPPPHRQCSSEPSILDSPDELELEDVASGSQEDSELNCASLS" } }, { "url": "https://www.uniprot.org/uniprot/A2AVJ5#B0R102-1", "isoform_ac": "B0R102-1", "locus": { "end_pos": 101772141, "strand": "+", "start_pos": 101772293, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000124802", "id": "ENSMUST00000124802", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000118502", "id": "ENSMUSP00000118502", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|B0R102-1|PRR5L_MOUSE Computationally mapped isoform of Proline-rich protein 5-like OS=Mus musculus OX=10090 GN=Prr5l PE=1 SV=1 CANONICAL=A2AVJ5-1", "length": 51, "sequence": "MTRGLAPLLPIEFHKMGSFRRPRPRFMSSPVLSELPRFQAARQALQLSSNS" } }, { "url": "https://www.uniprot.org/uniprot/A2AVJ5#B0R103-1", "isoform_ac": "B0R103-1", "locus": { "end_pos": 101772186, "strand": "+", "start_pos": 101772293, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000144549", "id": "ENSMUST00000144549", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000116266", "id": "ENSMUSP00000116266", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|B0R103-1|PRR5L_MOUSE Computationally mapped isoform of Proline-rich protein 5-like OS=Mus musculus OX=10090 GN=Prr5l PE=1 SV=8 CANONICAL=A2AVJ5-1", "length": 36, "sequence": "MTRGLAPLLPIEFHKMGSFRRPRPRFMSSPVLSELP" } }, { "url": "https://www.uniprot.org/uniprot/A2AVJ5#B0R105-1", "isoform_ac": "B0R105-1", "locus": { "end_pos": 101758519, "strand": "+", "start_pos": 101772293, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000154525", "id": "ENSMUST00000154525", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000120192", "id": "ENSMUSP00000120192", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|B0R105-1|PRR5L_MOUSE Computationally mapped isoform of Proline-rich protein 5-like OS=Mus musculus OX=10090 GN=Prr5l PE=1 SV=1 CANONICAL=A2AVJ5-1", "length": 95, "sequence": "MTRGLAPLLPIEFHKMGSFRRPRPRFMSSPVLSELPRFQAARQALQLSSNSAWNSVQTAVINVFKGGGLQSNELYALNESIRRLLKSELGSFITD" } }, { "url": "https://www.uniprot.org/uniprot/A2AVJ5#B0R106-1", "isoform_ac": "B0R106-1", "locus": { "end_pos": 101741350, "strand": "+", "start_pos": 101772293, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000125985", "id": "ENSMUST00000125985", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000122996", "id": "ENSMUSP00000122996", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|B0R106-1|PRR5L_MOUSE Computationally mapped isoform of Proline-rich protein 5-like OS=Mus musculus OX=10090 GN=Prr5l PE=1 SV=8 CANONICAL=A2AVJ5-1", "length": 127, "sequence": "MTRGLAPLLPIEFHKMGSFRRPRPRFMSSPVLSELPRFQAARQALQLSSNSAWNSVQTAVINVFKGGGLQSNELYALNESIRRLLKSELGSFITDYFQNQLLAKGLSFVEEKIKLCEGDNRIEVLAE" } }, { "url": "https://www.uniprot.org/uniprot/A2AVJ5#F6VRG5-1", "isoform_ac": "F6VRG5-1", "locus": { "end_pos": 101717251, "strand": "+", "start_pos": 101797650, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000141814", "id": "ENSMUST00000141814", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000118537", "id": "ENSMUSP00000118537", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|F6VRG5-1|PRR5L_MOUSE Computationally mapped isoform of Proline-rich protein 5-like OS=Mus musculus OX=10090 GN=Prr5l PE=1 SV=8 CANONICAL=A2AVJ5-1", "length": 238, "sequence": "XKPGQDATRRRAELGEDLGRASERARREPAESLVSLQQENFPWNTGNFFLSAQSPRRRRAVGVRGSARVTHAGDPKGGQELTIRQISLLGFRDLVLLKVKLGDVLLLAQSKLPSSVIQMLLILQSVHEPTGPSEGYLQLEELVKQVVSPFLSISGDRSCSGPTYSLARRHSRVRPKVTVLNYASLMTTVGRPLNEMVLTPLTEQEGEAYLEKCGSVRRHTVANAHSDIQLLAMATMMH" } } ], "pathway": [ { "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:72446", "resource": "KEGG Pathway", "id": "mmu:72446", "name": "" } ], "function": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5#function", "annotation": "Associates with the mTORC2 complex that regulates cellular processes including survival and organization of the cytoskeleton (By similarity). Regulates the activity of the mTORC2 complex in a substrate-specific manner preventing for instance the specific phosphorylation of PKCs and thereby controlling cell migration (PubMed:22609986). Plays a role in the stimulation of ZFP36-mediated mRNA decay of several ZFP36-associated mRNAs, such as TNF-alpha and GM-CSF, in response to stress. Required for ZFP36 localization to cytoplasmic stress granule (SG) and P-body (PB) in response to stress.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5#function", "id": "A2AVJ5", "database": "UniProtKB" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077279.1", "annotation": "PRR5L degradation promotes mTORC2-mediated PKC-delta phosphorylation and cell migration downstream of Galpha12 in fibroblasts and pulmonary fibrosis. Publication Status: Online-Only", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077279.1", "id": "NP_001077279.1", "database": "RefSeq" } ] } ], "orthologs": [ { "uniprot_canonical_ac": "A1L1K1-1", "protein_name": "Proline-rich protein 5-like", "sequence": { "length": 370, "sequence": "MTRGLAPLLPIEFHKMGSFRRPRPRFMSSPLLSELPRFQAARQALQLSSNSAWNSVQTAVINVFKGGGLQSNELYALNESIRRLLKSELGSFITDYFQNQLLAKGLSFVEEKIKQCEGDNRIEVLAEVWDHFFTETLPTLQAIFYPVQGQELTIRQISLLGFRDLVLLKVKLGDLLLLSQSKLPSSVIQMLLILQSVHEPTGPSEGYLQLEELVKQVVSPFLGLSGDRSCSRPTHSLARRHSRVRPKVTVLNYASLMTTVGRPLNEMVLTPLTEQEGEAYLEKCGSVRRHTVANAHSDIQLLAMATMMHSGLGEESGSEDKHLLLPPTFPPPHRQCSSEPSILDSPDEMELEDVASGSQEDSELNCASLS" }, "evidence": [ { "url": "http://www.informatics.jax.org/homology/11753", "id": "11753", "database": "MGI" } ], "common_name": "Rat", "organism": "Rattus norvegicus", "tax_id": 10116 }, { "uniprot_canonical_ac": "Q6MZQ0-1", "protein_name": "Proline-rich protein 5-like", "sequence": { "length": 368, "sequence": "MTRGFAPILPVEFHKMGSFRRPRPRFMSSPVLSDLPRFQAARQALQLSSSSAWNSVQTAVINVFKGGGLQSNELYALNENIRRLLKSELGSFITDYFQNQLLAKGLFFVEEKIKLCEGENRIEVLAEVWDHFFTETLPTLQAIFYPVQGQELTIRQISLLGFRDLVLLKVKLGDLLLLAQSKLPSSIVQMLLILQSVHEPTGPSESYLQLEELVKQVVSPFLGISGDRSFSGPTYTLARRHSRVRPKVTVLNYASPITAVSRPLNEMVLTPLTEQEGEAYLEKCGSVRRHTVANAHSDIQLLAMATMMHSGLGEEASSENKCLLLPPSFPPPHRQCSSEPNITDNPDGLEEGARGSQEGSELNCASLS" }, "evidence": [ { "url": "http://www.informatics.jax.org/homology/11753", "id": "11753", "database": "MGI" } ], "common_name": "Human", "organism": "Homo sapiens", "tax_id": 9606 } ], "interactions": [], "synthesized_glycans": [], "snv": [], "cluster_types": [ { "name": "isoformset.uniprotkb", "label": "Isoforms" }, { "name": "homologset.mgi", "label": "MGI Homologs" } ], "refseq": { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077279.1", "ac": "NP_001077279.1", "name": "proline-rich protein 5-like", "summary": "" }, "go_annotation": { "categories": [ { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0031625", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "id": "A2AVJ5", "database": "UniProtKB" } ], "name": "ubiquitin protein ligase binding", "id": "GO:0031625" } ], "total": 1, "name": "Molecular Function" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005739", "pmid": "18614015", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "id": "A2AVJ5", "database": "UniProtKB" } ], "name": "mitochondrion", "id": "GO:0005739" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0031932", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "id": "A2AVJ5", "database": "UniProtKB" } ], "name": "TORC2 complex", "id": "GO:0031932" } ], "total": 2, "name": "Cellular Component" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0010762", "pmid": "22609986", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "id": "A2AVJ5", "database": "UniProtKB" } ], "name": "regulation of fibroblast migration", "id": "GO:0010762" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0001934", "pmid": "21413931", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "id": "A2AVJ5", "database": "UniProtKB" } ], "name": "positive regulation of protein phosphorylation", "id": "GO:0001934" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0014068", "pmid": "21413931", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "id": "A2AVJ5", "database": "UniProtKB" } ], "name": "positive regulation of phosphatidylinositol 3-kinase signaling", "id": "GO:0014068" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0038203", "pmid": "22609986", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "id": "A2AVJ5", "database": "UniProtKB" } ], "name": "TORC2 signaling", "id": "GO:0038203" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0090316", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "id": "A2AVJ5", "database": "UniProtKB" } ], "name": "positive regulation of intracellular protein transport", "id": "GO:0090316" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0001933", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "id": "A2AVJ5", "database": "UniProtKB" } ], "name": "negative regulation of protein phosphorylation", "id": "GO:0001933" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0034599", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "id": "A2AVJ5", "database": "UniProtKB" } ], "name": "cellular response to oxidative stress", "id": "GO:0034599" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0009968", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "id": "A2AVJ5", "database": "UniProtKB" } ], "name": "negative regulation of signal transduction", "id": "GO:0009968" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0061014", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "id": "A2AVJ5", "database": "UniProtKB" } ], "name": "positive regulation of mRNA catabolic process", "id": "GO:0061014" } ], "total": 9, "name": "Biological Process" } ] }, "gene_names": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/A2AVJ5", "type": "synonym", "resource": "RefSeq", "name": "2600010E01Rik; 4833411O04Rik; Protor-2; Protor2" }, { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "type": "synonym", "resource": "UniProtKB", "name": "Protor2" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/A2AVJ5", "type": "synonym", "resource": "RefSeq", "name": "Prr5l" }, { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "type": "recommended", "resource": "UniProtKB", "name": "Prr5l" } ], "pro_annotation": [], "crossref": [ { "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000032841", "id": "ENSMUSG00000032841", "database": "Bgee" }, { "url": "https://www.ncbi.nlm.nih.gov/gene/72446", "id": "72446", "database": "GeneID" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR013745", "id": "IPR013745", "database": "InterPro" }, { "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:72446", "id": "mmu:72446", "database": "KEGG Pathway" }, { "url": "http://www.informatics.jax.org/marker/MGI:1919696", "id": "1919696", "database": "MGI" }, { "url": "https://omabrowser.org/oma/group/SEIWDHY/", "id": "SEIWDHY", "database": "OMA" }, { "url": "http://www.orthodb.org/?query=1260193at2759", "id": "1260193at2759", "database": "OrthoDB" }, { "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR32428", "id": "PTHR32428", "database": "PANTHER" }, { "url": "http://pfam.xfam.org/family/PF08539", "id": "PF08539", "database": "Pfam" }, { "url": "http://research.bioinformatics.udel.edu/pro/entry/PR_A2AVJ5", "id": "PR_A2AVJ5", "database": "PRO" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077279.1", "id": "NP_001077279.1", "database": "RefSeq" } ], "expression_disease": [], "glycosylation": [], "mutagenesis": [], "disease": [], "phosphorylation": [ { "residue": "Ser", "kinase_gene_name": "Atxn7l3b", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/21183079", "id": "21183079", "database": "PubMed" } ], "start_pos": "28", "end_pos": "28", "kinase_uniprot_canonical_ac": "Q3UD01-1" }, { "residue": "Ser", "kinase_gene_name": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "id": "A2AVJ5", "database": "UniProtKB" } ], "start_pos": "28", "end_pos": "28", "kinase_uniprot_canonical_ac": "" } ], "mass": { "monoisotopic_mass": 40982.0, "chemical_mass": 40982.0 }, "expression_tissue": [], "gene": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "name": "Prr5l", "locus": { "end_pos": 101714285, "strand": "-", "start_pos": 101883027, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUSG00000032841", "id": "ENSMUSG00000032841", "database": "Ensembl Gene" } ] } } ], "protein_names": [ { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "type": "recommended", "resource": "UniProtKB", "name": "Proline-rich protein 5-like" }, { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "type": "synonym", "resource": "UniProtKB", "name": "Protein observed with Rictor-2" }, { "url": "https://www.uniprot.org/uniprot/A2AVJ5", "type": "synonym", "resource": "UniProtKB", "name": "Protor-2" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/A2AVJ5", "type": "synonym", "resource": "RefSeq", "name": "proline-rich protein 5-like" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/A2AVJ5", "type": "synonym", "resource": "RefSeq", "name": "protein observed with Rictor-2" } ] }