{
    "uniprot_canonical_ac": "A2AKM2-1", 
    "ptm_annotation": [], 
    "sequence": {
        "header": "sp|A2AKM2-1|HACD4_MOUSE Isoform 1 of Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4  OS=Mus musculus OX=10090 GN=Hacd4 PE=2 SV=2 CANONICAL=A2AKM2-1", 
        "length": 232, 
        "sequence": "MGPSVLPAWLQPRYRKNVYLFIYYLIQFCGHSWILANMTVRFFSFGKDSMADTFYAIGLVMRVCQSISLLELLHIYIGIESNQLFPRFLQLTERVIILFGVITSQEEVQEKCVVCVLFILWNLLDMVRYTYSMLSVIGTSYAALTWLSQTLWMPIYPLCVLAEAFTIYQSLPYFESFGTNSTVLPFDLSTCFPYVLKLYLMMLFIGMYFTYSHLYTERKDFLRVFSVKQKNV"
    }, 
    "uniprot_id": "HACD4_MOUSE", 
    "enzyme_annotation": [
        {
            "ec": "4.2.1.134", 
            "activity": ""
        }, 
        {
            "ec": "4.2.1.134", 
            "activity": "A very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain trans-2,3-dehydroacyl-CoA + H(2)O."
        }
    ], 
    "glycation": [], 
    "keywords": [], 
    "species": [
        {
            "common_name": "Mouse", 
            "evidence": [
                {
                    "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                    "id": "A2AKM2", 
                    "database": "UniProtKB"
                }
            ], 
            "name": "Mus musculus", 
            "taxid": 10090
        }
    ], 
    "publication": [
        {
            "reference": [
                {
                    "url": "https://www.ncbi.nlm.nih.gov/pubmed/26160855", 
                    "type": [
                        "PubMed"
                    ], 
                    "id": "26160855"
                }
            ], 
            "title": "HACD1, a regulator of membrane composition and fluidity, promotes myoblast fusion and skeletal muscle growth.", 
            "journal": "Journal of molecular cell biology", 
            "evidence": [
                {
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_080036.1", 
                    "id": "NP_080036.1", 
                    "database": "RefSeq"
                }
            ], 
            "authors": "Blondelle J, Ohno Y, Gache V, Guyot S, Storck S, Blanchard-Gutton N, Barthelemy I, Walmsley G, Rahier A, Gadin S, Maurer M, Guillaud L, Prola A, Ferry A, Aubin-Houzelstein G, Demarquoy J, Relaix F, Piercy RJ, Blot S, Kihara A, Tiret L, Pilot-Storck F", 
            "date": "2015"
        }
    ], 
    "uniprot_ac": "A2AKM2", 
    "site_annotation": [
        {
            "end_pos": 182, 
            "annotation": "n_glycosylation_sequon", 
            "start_pos": 180
        }, 
        {
            "end_pos": 39, 
            "annotation": "n_glycosylation_sequon", 
            "start_pos": 37
        }
    ], 
    "isoforms": [
        {
            "url": "https://www.uniprot.org/uniprot/A2AKM2#A2AKM2-1", 
            "isoform_ac": "A2AKM2-1", 
            "locus": {
                "end_pos": 88414267, 
                "strand": "+", 
                "start_pos": 88438870, 
                "chromosome": "4", 
                "evidence": [
                    {
                        "url": "https://www.ensembl.org/id/ENSMUST00000030221", 
                        "id": "ENSMUST00000030221", 
                        "database": "Ensembl Transcript"
                    }, 
                    {
                        "url": "https://www.ensembl.org/id/ENSMUSP00000030221", 
                        "id": "ENSMUSP00000030221", 
                        "database": "Ensembl Peptide"
                    }
                ]
            }, 
            "sequence": {
                "header": "sp|A2AKM2-1|HACD4_MOUSE Isoform 1 of Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4  OS=Mus musculus OX=10090 GN=Hacd4 PE=2 SV=2 CANONICAL=A2AKM2-1", 
                "length": 232, 
                "sequence": "MGPSVLPAWLQPRYRKNVYLFIYYLIQFCGHSWILANMTVRFFSFGKDSMADTFYAIGLVMRVCQSISLLELLHIYIGIESNQLFPRFLQLTERVIILFGVITSQEEVQEKCVVCVLFILWNLLDMVRYTYSMLSVIGTSYAALTWLSQTLWMPIYPLCVLAEAFTIYQSLPYFESFGTNSTVLPFDLSTCFPYVLKLYLMMLFIGMYFTYSHLYTERKDFLRVFSVKQKNV"
            }
        }, 
        {
            "url": "https://www.uniprot.org/uniprot/A2AKM2#A2AKM2-2", 
            "isoform_ac": "A2AKM2-2", 
            "locus": {}, 
            "sequence": {
                "header": "sp|A2AKM2-2|HACD4_MOUSE Isoform 2 of Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4  OS=Mus musculus OX=10090 GN=Hacd4 PE=2 SV=2 CANONICAL=A2AKM2-1", 
                "length": 247, 
                "sequence": "MGPSVLPAWLQPRYRKNVYLFIYYLIQFCGHSWILANMTVRFFSFGKDSMADTFYAIGLVMRVCQSISLLELLHIYIGIESNQLFPRFLQLTERVIILFGVITSQEEVQEKCVVCVLFILWNLLDMVRYTYSMLSVIGTSYAALTWLSQTLWMPIYPLCVLAEAFTIYQSLPYFESFGTNSTVLPFDLSTCFPYVLKLYLMMLFIGNAKMFLIKCKKLCLKVFHSPIHLLHVLNTGHTTRPTTRPDI"
            }
        }, 
        {
            "url": "https://www.uniprot.org/uniprot/A2AKM2#F7CPS1-1", 
            "isoform_ac": "F7CPS1-1", 
            "locus": {
                "end_pos": 88414265, 
                "strand": "+", 
                "start_pos": 88438928, 
                "chromosome": "4", 
                "evidence": [
                    {
                        "url": "https://www.ensembl.org/id/ENSMUST00000151280", 
                        "id": "ENSMUST00000151280", 
                        "database": "Ensembl Transcript"
                    }, 
                    {
                        "url": "https://www.ensembl.org/id/ENSMUSP00000122565", 
                        "id": "ENSMUSP00000122565", 
                        "database": "Ensembl Peptide"
                    }
                ]
            }, 
            "sequence": {
                "header": "tr|F7CPS1-1|HACD4_MOUSE Computationally mapped isoform of Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4  OS=Mus musculus OX=10090 GN=Hacd4 PE=2 SV=1 CANONICAL=A2AKM2-1", 
                "length": 252, 
                "sequence": "XLSQPTPAASRRCQRSRLWPMGPSVLPAWLQPRYRKNVYLFIYYLIQFCGHSWILANMTVRFFSFGKDSMADTFYAIGLVMRVCQSISLLELLHIYIGIESNQLFPRFLQLTERVIILFGVITSQEEVQEKCVVCVLFILWNLLDMVRYTYSMLSVIGTSYAALTWLSQTLWMPIYPLCVLAEAFTIYQSLPYFESFGTNSTVLPFDLSTCFPYVLKLYLMMLFIGMYFTYSHLYTERKDFLRVFSVKQKNV"
            }
        }, 
        {
            "url": "https://www.uniprot.org/uniprot/A2AKM2#Z4YMP4-1", 
            "isoform_ac": "Z4YMP4-1", 
            "locus": {
                "end_pos": 88398095, 
                "strand": "+", 
                "start_pos": 88438915, 
                "chromosome": "4", 
                "evidence": [
                    {
                        "url": "https://www.ensembl.org/id/ENSMUST00000132493", 
                        "id": "ENSMUST00000132493", 
                        "database": "Ensembl Transcript"
                    }, 
                    {
                        "url": "https://www.ensembl.org/id/ENSMUSP00000119411", 
                        "id": "ENSMUSP00000119411", 
                        "database": "Ensembl Peptide"
                    }
                ]
            }, 
            "sequence": {
                "header": "tr|Z4YMP4-1|HACD4_MOUSE Computationally mapped isoform of Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4  OS=Mus musculus OX=10090 GN=Hacd4 PE=2 SV=1 CANONICAL=A2AKM2-1", 
                "length": 262, 
                "sequence": "TPAASRRCQRSRLWPMGPSVLPAWLQPRYRKNVYLFIYYLIQFCGHSWILANMTVRFFSFGKDSMADTFYAIGLVMRVCQSISLLELLHIYIGIESNQLFPRFLQLTERVIILFGVITSQEEVQEKCVVCVLFILWNLLDMVRYTYSMLSVIGTSYAALTWLSQTLWMPIYPLCVLAEAFTIYQSLPYFESFGTNSTVLPFDLSTCFPYVLKLYLMMLFIGNAKMFLIKCKKLCLKVFHSPIHLLHVLNTGHTTRPTTRPDI"
            }
        }
    ], 
    "pathway": [
        {
            "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:66775", 
            "resource": "KEGG Pathway", 
            "id": "mmu:66775", 
            "name": ""
        }, 
        {
            "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-75876", 
            "resource": "Reactome", 
            "id": "R-MMU-75876", 
            "name": "Synthesis of very long-chain fatty acyl-CoAs"
        }
    ], 
    "function": [
        {
            "url": "https://www.uniprot.org/uniprot/A2AKM2#function", 
            "annotation": "Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.", 
            "evidence": [
                {
                    "url": "https://www.uniprot.org/uniprot/A2AKM2#function", 
                    "id": "A2AKM2", 
                    "database": "UniProtKB"
                }
            ]
        }
    ], 
    "orthologs": [
        {
            "uniprot_canonical_ac": "D3Z8V6-1", 
            "protein_name": "Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase", 
            "sequence": {
                "length": 232, 
                "sequence": "MGPSVLPAWLQPRYRKSAYLFIYYLIQFCGHSWILTNMTVRFFSFGKDSMVDTFYAIGLVMRVCQSISLLELLHIYIGIESNQLFPKFLQLTERIIILFGVITSQEEVQEKYVVCVLFIFWNLLDMVRYTYSMLSVIGTSYAVLTWLSQTLWMPIYPLCVLAEAFTIYQSLPYFELFGKNSTMLPFDISTYFPYVLKLYLMMLFIGMYFTYSHLYTERKDILRGSSVKQKKM"
            }, 
            "evidence": [
                {
                    "url": "http://www.informatics.jax.org/homology/12033", 
                    "id": "12033", 
                    "database": "MGI"
                }, 
                {
                    "url": "https://omabrowser.org/oma/group/A2AKM2/", 
                    "id": "A2AKM2", 
                    "database": "OMA"
                }
            ], 
            "common_name": "Rat", 
            "organism": "Rattus norvegicus", 
            "tax_id": 10116
        }, 
        {
            "uniprot_canonical_ac": "Q5VWC8-1", 
            "protein_name": "Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4", 
            "sequence": {
                "length": 232, 
                "sequence": "MGPLALPAWLQPRYRKNAYLFIYYLIQFCGHSWIFTNMTVRFFSFGKDSMVDTFYAIGLVMRLCQSVSLLELLHIYVGIESNHLLPRFLQLTERIIILFVVITSQEEVQEKYVVCVLFVFWNLLDMVRYTYSMLSVIGISYAVLTWLSQTLWMPIYPLCVLAEAFAIYQSLPYFESFGTYSTKLPFDLSIYFPYVLKIYLMMLFIGMYFTYSHLYSERRDILGIFPIKKKKM"
            }, 
            "evidence": [
                {
                    "url": "http://www.informatics.jax.org/homology/12033", 
                    "id": "12033", 
                    "database": "MGI"
                }, 
                {
                    "url": "https://omabrowser.org/oma/group/A2AKM2/", 
                    "id": "A2AKM2", 
                    "database": "OMA"
                }
            ], 
            "common_name": "Human", 
            "organism": "Homo sapiens", 
            "tax_id": 9606
        }
    ], 
    "interactions": [], 
    "synthesized_glycans": [], 
    "snv": [], 
    "cluster_types": [
        {
            "name": "isoformset.uniprotkb", 
            "label": "Isoforms"
        }, 
        {
            "name": "homologset.mgi", 
            "label": "MGI Homologs"
        }, 
        {
            "name": "homologset.oma", 
            "label": "OMA Homologs"
        }
    ], 
    "refseq": {
        "url": "https://www.ncbi.nlm.nih.gov/protein/NP_080036.1", 
        "ac": "NP_080036.1", 
        "name": "very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4", 
        "summary": ""
    }, 
    "go_annotation": {
        "categories": [
            {
                "go_terms": [
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0019899", 
                        "pmid": "", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "enzyme binding", 
                        "id": "GO:0019899"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0018812", 
                        "pmid": "", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "3-hydroxyacyl-CoA dehydratase activity", 
                        "id": "GO:0018812"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0102158", 
                        "pmid": "", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "very-long-chain 3-hydroxyacyl-CoA dehydratase activity", 
                        "id": "GO:0102158"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0102343", 
                        "pmid": "", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "3-hydroxy-arachidoyl-CoA dehydratase activity", 
                        "id": "GO:0102343"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0102344", 
                        "pmid": "", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "3-hydroxy-behenoyl-CoA dehydratase activity", 
                        "id": "GO:0102344"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0102345", 
                        "pmid": "", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "3-hydroxy-lignoceroyl-CoA dehydratase activity", 
                        "id": "GO:0102345"
                    }
                ], 
                "total": 6, 
                "name": "Molecular Function"
            }, 
            {
                "go_terms": [
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0030176", 
                        "pmid": "21873635", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "integral component of endoplasmic reticulum membrane", 
                        "id": "GO:0030176"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005783", 
                        "pmid": "", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "endoplasmic reticulum", 
                        "id": "GO:0005783"
                    }
                ], 
                "total": 2, 
                "name": "Cellular Component"
            }, 
            {
                "go_terms": [
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0030148", 
                        "pmid": "21873635", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "sphingolipid biosynthetic process", 
                        "id": "GO:0030148"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0030497", 
                        "pmid": "", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "fatty acid elongation", 
                        "id": "GO:0030497"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0042761", 
                        "pmid": "", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "very long-chain fatty acid biosynthetic process", 
                        "id": "GO:0042761"
                    }
                ], 
                "total": 3, 
                "name": "Biological Process"
            }
        ]
    }, 
    "gene_names": [
        {
            "url": "https://www.ncbi.nlm.nih.gov/protein/A2AKM2", 
            "type": "synonym", 
            "resource": "RefSeq", 
            "name": "4933428I03Rik; Hcad4; Ptplad2"
        }, 
        {
            "url": "https://www.ncbi.nlm.nih.gov/protein/A2AKM2", 
            "type": "synonym", 
            "resource": "RefSeq", 
            "name": "Hacd4"
        }, 
        {
            "url": "https://www.uniprot.org/uniprot/A2AKM2", 
            "type": "recommended", 
            "resource": "UniProtKB", 
            "name": "Hacd4"
        }, 
        {
            "url": "https://www.uniprot.org/uniprot/A2AKM2", 
            "type": "synonym", 
            "resource": "UniProtKB", 
            "name": "Ptplad2"
        }
    ], 
    "pro_annotation": [], 
    "crossref": [
        {
            "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000028497", 
            "id": "ENSMUSG00000028497", 
            "database": "Bgee"
        }, 
        {
            "url": "https://www.ncbi.nlm.nih.gov/gene/66775", 
            "id": "66775", 
            "database": "GeneID"
        }, 
        {
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR007482", 
            "id": "IPR007482", 
            "database": "InterPro"
        }, 
        {
            "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:66775", 
            "id": "mmu:66775", 
            "database": "KEGG Pathway"
        }, 
        {
            "url": "http://www.informatics.jax.org/marker/MGI:1914025", 
            "id": "1914025", 
            "database": "MGI"
        }, 
        {
            "url": "http://www.orthodb.org/?query=1458293at2759", 
            "id": "1458293at2759", 
            "database": "OrthoDB"
        }, 
        {
            "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR11035", 
            "id": "PTHR11035", 
            "database": "PANTHER"
        }, 
        {
            "url": "http://pfam.xfam.org/family/PF04387", 
            "id": "PF04387", 
            "database": "Pfam"
        }, 
        {
            "url": "http://research.bioinformatics.udel.edu/pro/entry/PR_A2AKM2", 
            "id": "PR_A2AKM2", 
            "database": "PRO"
        }, 
        {
            "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-75876", 
            "id": "R-MMU-75876", 
            "database": "Reactome"
        }, 
        {
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_080036.1", 
            "id": "NP_080036.1", 
            "database": "RefSeq"
        }
    ], 
    "expression_disease": [], 
    "glycosylation": [], 
    "mutagenesis": [], 
    "disease": [], 
    "phosphorylation": [], 
    "mass": {
        "monoisotopic_mass": 27235.0, 
        "chemical_mass": 27235.0
    }, 
    "expression_tissue": [], 
    "gene": [
        {
            "url": "https://www.uniprot.org/uniprot/A2AKM2", 
            "name": "Hacd4", 
            "locus": {
                "end_pos": 88396144, 
                "strand": "-", 
                "start_pos": 88438928, 
                "chromosome": "4", 
                "evidence": [
                    {
                        "url": "https://www.ensembl.org/id/ENSMUSG00000028497", 
                        "id": "ENSMUSG00000028497", 
                        "database": "Ensembl Gene"
                    }
                ]
            }
        }
    ], 
    "protein_names": [
        {
            "url": "https://www.uniprot.org/uniprot/A2AKM2", 
            "type": "synonym", 
            "resource": "UniProtKB", 
            "name": "3-hydroxyacyl-CoA dehydratase 4"
        }, 
        {
            "url": "https://www.uniprot.org/uniprot/A2AKM2", 
            "type": "synonym", 
            "resource": "UniProtKB", 
            "name": "4.2.1.134"
        }, 
        {
            "url": "https://www.uniprot.org/uniprot/A2AKM2", 
            "type": "synonym", 
            "resource": "UniProtKB", 
            "name": "HACD4"
        }, 
        {
            "url": "https://www.uniprot.org/uniprot/A2AKM2", 
            "type": "synonym", 
            "resource": "UniProtKB", 
            "name": "Protein-tyrosine phosphatase-like A domain-containing protein 2"
        }, 
        {
            "url": "https://www.uniprot.org/uniprot/A2AKM2", 
            "type": "recommended", 
            "resource": "UniProtKB", 
            "name": "Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4"
        }, 
        {
            "url": "https://www.ncbi.nlm.nih.gov/protein/A2AKM2", 
            "type": "synonym", 
            "resource": "RefSeq", 
            "name": "protein tyrosine phosphatase-like protein PTPLAD2; protein-tyrosine phosphatase-like A domain-containing protein 2; very-long-chain (3R)-3-hydroxyacyl-[acyl-carrier protein] dehydratase 4; protein tyrosine phosphatase-like A domain containing 2"
        }, 
        {
            "url": "https://www.ncbi.nlm.nih.gov/protein/A2AKM2", 
            "type": "synonym", 
            "resource": "RefSeq", 
            "name": "very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4"
        }
    ]
}