{ "uniprot_canonical_ac": "A1L314-1", "ptm_annotation": [ { "annotation": "Monoubiquitinated in response to bacterial infection; ubiquitination is required for vesicular localization and antibacterial activity and can be blocked by bacterial cell cycle inhibiting factor (cif) (PubMed:26418746).", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" }, { "url": "https://www.ncbi.nlm.nih.gov/pubmed/26418746", "id": "26418746", "database": "PubMed" } ] } ], "sequence": { "header": "sp|A1L314-1|MPEG1_MOUSE Isoform 1 of Macrophage-expressed gene 1 protein OS=Mus musculus OX=10090 GN=Mpeg1 PE=1 SV=1 CANONICAL=A1L314-1", "length": 713, "sequence": "MNSFMALVLIWMIIACAEADKPLGETGTTGFQICKNALKLPVLEVLPGGGWDNLRNVDMGRVMDLTYTNCKTTEDGQYIIPDEVYTIPQKESNLEMNSEVLESWMNYQSTTSLSINTELALFSRVNGKFSTEFQRMKTLQVKDQAVTTRVQVRNRIYTVKTTPTSELSLGFTKALMDICDQLEKNQTKMATYLAELLILNYGTHVITSVDAGAALVQEDHVRSSFLLDNQNSQNTVTASAGIAFLNIVNFKVETDYISQTSLTKDYLSNRTNSRVQSFGGVPFYPGITLETWQKGITNHLVAIDRAGLPLHFFIKPDKLPGLPGPLVKKLSKTVETAVRHYYTFNTHPGCTNVDSPNFNFQANMDDDSCDAKVTNFTFGGVYQECTELSGDVLCQNLEQKNLLTGDFSCPPGYSPVHLLSQTHEEGYSRLECKKKCTLKIFCKTVCEDVFRVAKAEFRAYWCVAAGQVPDNSGLLFGGVFTDKTINPMTNAQSCPAGYIPLNLFESLKVCVSLDYELGFKFSVPFGGFFSCIMGNPLVNSDTAKDVRAPSLKKCPGGFSQHLAVISDGCQVSYCVKAGIFTGGSLLPVRLPPYTKPPLMSQVATNTVIVTNSETARSWIKDPQTNQWKLGEPLELRRAMTVIHGDSNGMSGGEAAGITLGVTIALGIVITLAIYGTRKYKKKEYQEIEEQESLVGSLATDATVLNGEEDPSPA" }, "uniprot_id": "MPEG1_MOUSE", "enzyme_annotation": [], "glycation": [], "keywords": [ "glycoprotein", "predicted.uniprotkb_gly" ], "species": [ { "common_name": "Mouse", "taxid": 10090, "name": "Mus musculus", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" } ] } ], "crossref_internal": { "gene": [ "Mpeg1" ], "site": [ "A1L314-1.678.681", "A1L314-1.375.375", "A1L314-1.375.377", "A1L314-1.269.271", "A1L314-1.185.185", "A1L314-1.185.187", "A1L314-1.269.269" ], "species": [ 10090 ] }, "publication": [ { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/23257510", "type": [ "PubMed" ], "id": "23257510" } ], "title": "Inhibition of intracellular bacterial replication in fibroblasts is dependent on the perforin-like protein (perforin-2) encoded by macrophage-expressed gene 1.", "journal": "J. Innate Immun.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" } ], "authors": "Shiratsuchi M., Podack E.R., McCormack R., de Armas L.R., Ramos J.E.", "date": "2013" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/23753625", "type": [ "PubMed" ], "id": "23753625" } ], "title": "Perforin-2 restricts growth of Chlamydia trachomatis in macrophages.", "journal": "Infect. Immun.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" } ], "authors": "Podack E.R., Fields K.A., McCormack R., de Armas L.R.", "date": "2013" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/26402460", "type": [ "PubMed" ], "id": "26402460" } ], "title": "Perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria.", "journal": "Elife", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" } ], "authors": "Olsson M.L., Morales A., Shiratsuchi M., Podack E.R., Sukumar N., Lichtenheld M.G., Strbo N., Fiorentino D.G., Gonzalez L.E., Kirsner R.S., Lyapichev K., McCormack R.M., Munson G.P., Plano G.V., Russell D.G., Stojadinovic O., Tomic-Canic M., de Armas L.R.", "date": "2015" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/26418746", "type": [ "PubMed" ], "id": "26418746" } ], "title": "Enteric pathogens deploy cell cycle inhibiting factors to block the bactericidal activity of Perforin-2.", "journal": "Elife", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" } ], "authors": "Olsson M.L., Podack E.R., Lyapichev K., McCormack R.M., Munson G.P.", "date": "2015" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/26831467", "type": [ "PubMed" ], "id": "26831467" } ], "title": "Perforin-2 protects host cells and mice by restricting the vacuole to cytosol transitioning of a bacterial pathogen.", "journal": "Infect. Immun.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" } ], "authors": "Podack E.R., Khan W.N., Barreto M., Boucher J., McCormack R., Bahnan W., Barrera C.M., Dauer E.A., Freitag N.E., Schesser K., Shrestha N.", "date": "2016" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/30249808", "type": [ "PubMed" ], "id": "30249808" } ], "title": "Perforin-2 breaches the envelope of phagocytosed bacteria allowing antimicrobial effectors access to intracellular targets.", "journal": "J. Immunol.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" } ], "authors": "Bai F., Lichtenheld M.G., McCormack R.M., Munson G.P., Plano G.V., Hower S.", "date": "2018" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/7888681", "type": [ "PubMed" ], "id": "7888681" } ], "title": "Isolation of a novel macrophage-specific gene by differential cDNA analysis.", "journal": "Blood", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" } ], "authors": "Takayama Y., Wu W.M., Rowe P.B., O'Mara M.-A., Spilsbury K., Symonds G.", "date": "1995" } ], "uniprot_ac": "A1L314", "site_annotation": [ { "end_pos": 187, "annotation": "n_glycosylation_sequon", "start_pos": 185 }, { "end_pos": 271, "annotation": "n_glycosylation_sequon", "start_pos": 269 }, { "end_pos": 377, "annotation": "n_glycosylation_sequon", "start_pos": 375 } ], "isoforms": [ { "url": "https://www.uniprot.org/uniprot/A1L314#A1L314-1", "isoform_ac": "A1L314-1", "sequence": { "header": "sp|A1L314-1|MPEG1_MOUSE Isoform 1 of Macrophage-expressed gene 1 protein OS=Mus musculus OX=10090 GN=Mpeg1 PE=1 SV=1 CANONICAL=A1L314-1", "length": 713, "sequence": "MNSFMALVLIWMIIACAEADKPLGETGTTGFQICKNALKLPVLEVLPGGGWDNLRNVDMGRVMDLTYTNCKTTEDGQYIIPDEVYTIPQKESNLEMNSEVLESWMNYQSTTSLSINTELALFSRVNGKFSTEFQRMKTLQVKDQAVTTRVQVRNRIYTVKTTPTSELSLGFTKALMDICDQLEKNQTKMATYLAELLILNYGTHVITSVDAGAALVQEDHVRSSFLLDNQNSQNTVTASAGIAFLNIVNFKVETDYISQTSLTKDYLSNRTNSRVQSFGGVPFYPGITLETWQKGITNHLVAIDRAGLPLHFFIKPDKLPGLPGPLVKKLSKTVETAVRHYYTFNTHPGCTNVDSPNFNFQANMDDDSCDAKVTNFTFGGVYQECTELSGDVLCQNLEQKNLLTGDFSCPPGYSPVHLLSQTHEEGYSRLECKKKCTLKIFCKTVCEDVFRVAKAEFRAYWCVAAGQVPDNSGLLFGGVFTDKTINPMTNAQSCPAGYIPLNLFESLKVCVSLDYELGFKFSVPFGGFFSCIMGNPLVNSDTAKDVRAPSLKKCPGGFSQHLAVISDGCQVSYCVKAGIFTGGSLLPVRLPPYTKPPLMSQVATNTVIVTNSETARSWIKDPQTNQWKLGEPLELRRAMTVIHGDSNGMSGGEAAGITLGVTIALGIVITLAIYGTRKYKKKEYQEIEEQESLVGSLATDATVLNGEEDPSPA" }, "locus": {} }, { "url": "https://www.uniprot.org/uniprot/A1L314#E9QN37-1", "isoform_ac": "E9QN37-1", "sequence": { "header": "tr|E9QN37-1|MPEG1_MOUSE Computationally mapped isoform of Macrophage-expressed gene 1 protein OS=Mus musculus OX=10090 GN=Mpeg1 PE=1 SV=1 CANONICAL=A1L314-1", "length": 720, "sequence": "MAKTICAMNSFMALVLIWMIIACAEADKPLGETGTTGFQICKNALKLPVLEVLPGGGWDNLRNVDMGRVMDLTYTNCKTTEDGQYIIPDEVYTIPQKESNLEMNSEVLESWMNYQSTTSLSINTELALFSRVNGKFSTEFQRMKTLQVKDQAVTTRVQVRNRIYTVKTTPTSELSLGFTKALMDICDQLEKNQTKMATYLAELLILNYGTHVITSVDAGAALVQEDHVRSSFLLDNQNSQNTVTASAGIAFLNIVNFKVETDYISQTSLTKDYLSNRTNSRVQSFGGVPFYPGITLETWQKGITNHLVAIDRAGLPLHFFIKPDKLPGLPGPLVKKLSKTVETAVRHYYTFNTHPGCTNVDSPNFNFQANMDDDSCDAKVTNFTFGGVYQECTELSGDVLCQNLEQKNLLTGDFSCPPGYTPVHLLSQTHEEGYSRLECKKKCTLKIFCKTVCEDVFRVAKAEFRAYWCVAAGQVPDNSGLLFGGVFTDKTINPMTNAQSCPAGYIPLNLFESLKVCVSLDYELGFKFSVPFGGFFSCIMGNPLVNSDTAKDVRAPSLKKCPGGFSQHLAVISDGCQVSYCVKAGIFTGGSLLPVRLPPYTKPPLMSQVATNTVIVTNSETARSWIKDPQTNQWKLGEPLELRRAMTVIHGDSNGMSGGEAAGITLGVTIALGVVITLAIYGTRKYKKKEYQEIEEQESLVGSLATDATVLNGEEDPSPA" }, "locus": { "chromosome": "19", "end_pos": 12461180, "start_pos": 12463339, "strand": "-", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000081035", "id": "ENSMUST00000081035", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000108573", "id": "ENSMUSP00000108573", "database": "Ensembl Peptide" } ] } } ], "pathway": [ { "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:17476", "resource": "KEGG Pathway", "id": "mmu:17476", "name": "" } ], "function": [ { "url": "https://www.uniprot.org/uniprot/A1L314#function", "annotation": "Plays a key role in the innate immune response following bacterial infection by polymerizing and inserting into the bacterial surface to form pores (PubMed:26402460). By breaching the surface of phagocytosed bacteria, allows antimicrobial effectors to enter the bacterial periplasmic space and degrade bacterial proteins such as superoxide dismutase sodC which contributes to bacterial virulence (PubMed:30249808). Shows antibacterial activity against a wide spectrum of Gram-positive, Gram-negative and acid-fast bacteria (PubMed:23257510, PubMed:23753625, PubMed:26402460). Reduces the viability of the intracytosolic pathogen L.monocytogenes by inhibiting acidification of the phagocytic vacuole of host cells which restricts bacterial translocation from the vacuole to the cytosol (PubMed:26831467). Required for the antibacterial activity of reactive oxygen species and nitric oxide (PubMed:26402460).", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314#function", "id": "A1L314", "database": "UniProtKB" } ] } ], "orthologs": [ { "uniprot_canonical_ac": "Q2M385-1", "protein_name": "Macrophage-expressed gene 1 protein", "sequence": { "length": 716, "sequence": "MNNFRATILFWAAAAWAKSGKPSGEMDEVGVQKCKNALKLPVLEVLPGGGWDNLRNVDMGRVMELTYSNCRTTEDGQYIIPDEIFTIPQKQSNLEMNSEILESWANYQSSTSYSINTELSLFSKVNGKFSTEFQRMKTLQVKDQAITTRVQVRNLVYTVKINPTLELSSGFRKELLDISDRLENNQTRMATYLAELLVLNYGTHVTTSVDAGAALIQEDHLRASFLQDSQSSRSAVTASAGLAFQNTVNFKFEENYTSQNVLTKSYLSNRTNSRVQSIGGVPFYPGITLQAWQQGITNHLVAIDRSGLPLHFFINPNMLPDLPGPLVKKVSKTVETAVKRYYTFNTYPGCTDLNSPNFNFQANTDDGSCEGKMTNFSFGGVYQECTQLSGNRDVLLCQKLEQKNPLTGDFSCPSGYSPVHLLSQIHEEGYNHLECHRKCTLLVFCKTVCEDVFQVAKAEFRAFWCVASSQVPENSGLLFGGLFSSKSINPMTNAQSCPAGYFPLRLFENLKVCVSQDYELGSRFAVPFGGFFSCTVGNPLVDPAISRDLGAPSLKKCPGGFSQHPALISDGCQVSYCVKSGLFTGGSLPPARLPPFTRPPLMSQAATNTVIVTNSENARSWIKDSQTHQWRLGEPIELRRAMNVIHGDGGGLSGGAAAGVTVGVTTILAVVITLAIYGTRKFKKKAYQAIEERQSLVPGTAATGDTTYQEQGQSPA" }, "evidence": [ { "url": "http://www.informatics.jax.org/homology/11216", "id": "11216", "database": "MGI" } ], "common_name": "Human", "organism": "Homo sapiens", "tax_id": 9606 } ], "interactions": [], "synthesized_glycans": [], "snv": [], "cluster_types": [ { "name": "isoformset.uniprotkb", "label": "Isoforms" }, { "name": "homologset.mgi", "label": "MGI Homologs" } ], "refseq": {}, "go_annotation": { "categories": [ { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0031410", "pmid": "26402460", "id": "GO:0031410", "name": "cytoplasmic vesicle", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" } ] }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0045335", "pmid": "26402460", "id": "GO:0045335", "name": "phagocytic vesicle", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" } ] }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0016021", "pmid": "", "id": "GO:0016021", "name": "integral component of membrane", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" } ] }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0030659", "pmid": "", "id": "GO:0030659", "name": "cytoplasmic vesicle membrane", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" } ] } ], "total": 4, "name": "Cellular Component" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0050829", "pmid": "23257510", "id": "GO:0050829", "name": "defense response to Gram-negative bacterium", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" } ] }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0050829", "pmid": "23753625", "id": "GO:0050829", "name": "defense response to Gram-negative bacterium", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" } ] }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0050830", "pmid": "23257510", "id": "GO:0050830", "name": "defense response to Gram-positive bacterium", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" } ] }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0042742", "pmid": "26402460", "id": "GO:0042742", "name": "defense response to bacterium", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" } ] }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0035915", "pmid": "26402460", "id": "GO:0035915", "name": "pore formation in membrane of other organism", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" } ] }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0045087", "pmid": "", "id": "GO:0045087", "name": "innate immune response", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" } ] } ], "total": 6, "name": "Biological Process" } ] }, "gene_names": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "resource": "UniProtKB", "type": "recommended", "name": "Mpeg1" } ], "pro_annotation": [], "crossref": [ { "url": "https://www.ncbi.nlm.nih.gov/gene/17476", "id": "17476", "database": "GeneID" }, { "url": "https://acgg.asia/gpdb2/GPDB0001579", "id": "GPDB0001579", "database": "GlycoProtDB" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR020864", "id": "IPR020864", "database": "InterPro" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR039707", "id": "IPR039707", "database": "InterPro" }, { "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:17476", "id": "mmu:17476", "database": "KEGG Pathway" }, { "url": "http://www.informatics.jax.org/marker/MGI:1333743", "id": "1333743", "database": "MGI" }, { "url": "http://www.orthodb.org/?query=235876at2759", "id": "235876at2759", "database": "OrthoDB" }, { "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR31463", "id": "PTHR31463", "database": "PANTHER" }, { "url": "http://pfam.xfam.org/family/PF01823", "id": "PF01823", "database": "Pfam" }, { "url": "http://research.bioinformatics.udel.edu/pro/entry/PR_A1L314", "id": "PR_A1L314", "database": "PRO" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_034951.1", "id": "NP_034951.1", "database": "RefSeq" } ], "expression_disease": [], "glycosylation": [ { "residue": "Asn", "site_category": "predicted", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314#ptm_processing", "id": "A1L314", "database": "UniProtKB" } ], "subtype": "", "relation": "attached", "position": 185, "glytoucan_ac": "", "type": "N-linked" }, { "residue": "Asn", "site_category": "predicted", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314#ptm_processing", "id": "A1L314", "database": "UniProtKB" } ], "subtype": "", "relation": "attached", "position": 269, "glytoucan_ac": "", "type": "N-linked" }, { "residue": "Asn", "site_category": "predicted", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314#ptm_processing", "id": "A1L314", "database": "UniProtKB" } ], "subtype": "", "relation": "attached", "position": 375, "glytoucan_ac": "", "type": "N-linked" } ], "mutagenesis": [ { "sequence_org": "KYKK", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "id": "A1L314", "database": "UniProtKB" }, { "url": "https://www.ncbi.nlm.nih.gov/pubmed/26418746", "id": "26418746", "database": "PubMed" } ], "sequence_mut": "QYQQ", "start_pos": 678, "end_pos": 681, "annotation": "Abolishes ubiquitination and antibacterial activity and results in diffuse perinuclear distribution." } ], "disease": [], "phosphorylation": [], "mass": { "monoisotopic_mass": 78390.0, "chemical_mass": 78390.0 }, "expression_tissue": [], "gene": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "name": "Mpeg1", "locus": { "chromosome": "19", "end_pos": 12465284, "start_pos": 12460779, "strand": "+", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUSG00000046805", "id": "ENSMUSG00000046805", "database": "Ensembl Gene" } ] } } ], "protein_names": [ { "url": "https://www.uniprot.org/uniprot/A1L314", "resource": "UniProtKB", "type": "synonym", "name": "Macrophage gene 1 protein" }, { "url": "https://www.uniprot.org/uniprot/A1L314", "resource": "UniProtKB", "type": "recommended", "name": "Macrophage-expressed gene 1 protein" }, { "url": "https://www.uniprot.org/uniprot/A1L314", "resource": "UniProtKB", "type": "synonym", "name": "Mpg-1" }, { "url": "https://www.uniprot.org/uniprot/A1L314", "resource": "UniProtKB", "type": "synonym", "name": "P-2" }, { "url": "https://www.uniprot.org/uniprot/A1L314", "resource": "UniProtKB", "type": "synonym", "name": "Perforin-2" }, { "url": "https://www.uniprot.org/uniprot/A1L314", "resource": "UniProtKB", "type": "synonym", "name": "Protein MPS1" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/A1L314", "resource": "RefSeq", "type": "synonym", "name": "macrophage gene 1 protein; macrophage-expressed gene 1 protein; perforin-2" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/A1L314", "resource": "RefSeq", "type": "synonym", "name": "macrophage-expressed gene 1 protein precursor" } ] }