{ "uniprot_canonical_ac": "A2A935-1", "ptm_annotation": [], "sequence": { "header": "sp|A2A935-1|PRD16_MOUSE Isoform 1 of Histone-lysine N-methyltransferase PRDM16 OS=Mus musculus OX=10090 GN=Prdm16 PE=1 SV=1 CANONICAL=A2A935-1", "length": 1275, "sequence": "MRSKARARKLAKSDGDVVNNMYEPDPDLLAGQSAEEETEDGILSPIPMGPPSPFPTSEDFTPKEGSPYEAPVYIPEDIPIPPDFELRESSIPGAGLGIWAKRKMEIGERFGPYVVTPRAALKEADFGWEMLTDTEVSSQESCIKKQISEDLGSEKFCVDANQAGSGSWLKYIRVACSCDDQNLAMCQINEQIYYKVIKDIEPGEELLVHVKEGAYSLGVMAPSLDEDPTFRCDECDELFQCRLDLRRHKKYACSSAGAQLYEGLGEELKPEGLGVGSDGQAHECKDCERMFPNKYSLEQHMIVHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHSTVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHYTPGSIFTPGLPLTPSPMMDKTKPSPTLNHGGLGFSEYFPSRPHPGSLPFSAAPPAFPALTPGFPGIFPPSLYPRPPLLPPTPLLKSPLNHAQDAKLPSPLGNPALPLVSAVSNSSQGATAATGSEEKFDGRLEDAYAEKVKNRSPDMSDGSDFEDINTTTGTDLDTTTGTGSDLDSDLDSDRDKGKDKGKPVESKPEFGGASVPPGAMNSVAEVPAFYSQHSFFPPPEEQLLTASGAAGDSIKAIASIAEKYFGPGFMSMQEKKLGSLPYHSVFPFQFLPNFPHSLYPFTDRALAHNLLVKAEPKSPRDALKVGGPSAECPFDLTTKPKEAKPALLAPKVPLIPSSGEEQPLDLSIGSRARASQNGGGREPRKNHVYGERKPGVSEGLPKVCPAQLPQQPSLHYAKPSPFFMDPIYRVEKRKVADPVGVLKEKYLRPSPLLFHPQMSAIETMTEKLESFAAMKADSGSSLQPLPHHPFNFRSPPPTLSDPILRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKHEHEGAPVSQHSGVLTNHLGTSASSPTSESDNHALLDEKEDSYFSEIRNFIANSEMNQASTRMDKRPEIQDLDSNPPCPGSASAKPEDVEEEEEEELEEEDDDSLAGKSQEDTVSPTPEPQGVYEDEEDEEPPSLTMGFDHTRRCVEERGGGLLALEPTPTFGKGLDLRRAAEEAFEVKDVLNSTLDSEVLKQTLYRQAKNQAYAMMLSLSEDTPLHAPSQSSLDAWLNITGPSSESGAFNPINHL" }, "uniprot_id": "PRD16_MOUSE", "enzyme_annotation": [ { "ec": "2.1.1.-", "activity": "" } ], "glycation": [], "keywords": [], "species": [ { "common_name": "Mouse", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "name": "Mus musculus", "taxid": 10090 } ], "publication": [ { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/11818962", "type": [ "PubMed" ], "id": "11818962" } ], "title": "Efficient generation and mapping of recessive developmental mutations using ENU mutagenesis.", "journal": "Nature genetics", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277955.1", "id": "NP_001277955.1", "database": "RefSeq" } ], "authors": "Herron BJ, Lu W, Rao C, Liu S, Peters H, Bronson RT, Justice MJ, McDonald JD, Beier DR", "date": "2002" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/12816872", "type": [ "PubMed" ], "id": "12816872" } ], "title": "A novel EVI1 gene family, MEL1, lacking a PR domain (MEL1S) is expressed mainly in t(1;3)(p36;q21)-positive AML and blocks G-CSF-induced myeloid differentiation.", "journal": "Blood", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277955.1", "id": "NP_001277955.1", "database": "RefSeq" } ], "authors": "Nakamura T., Sasaki H., Asou N., Morishita K., Iga M., Imayoshi S., Nishikata I., Tateno Y.", "date": "2003" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/16109773", "type": [ "PubMed" ], "id": "16109773" } ], "title": "Insertional mutagenesis identifies genes that promote the immortalization of primary bone marrow progenitor cells.", "journal": "Blood", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277955.1", "id": "NP_001277955.1", "database": "RefSeq" } ], "authors": "Du Y, Jenkins NA, Copeland NG", "date": "2005" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/17467076", "type": [ "PubMed" ], "id": "17467076" } ], "title": "PRDM16/MEL1: a novel Smad binding protein expressed in murine embryonic orofacial tissue.", "journal": "Biochim. Biophys. Acta", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277955.1", "id": "NP_001277955.1", "database": "RefSeq" } ], "authors": "Warner D.R., Pisano M.M., Webb C.L., Greene R.M., Horn K.H., Mudd L.", "date": "2007" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/17618855", "type": [ "PubMed" ], "id": "17618855" } ], "title": "Transcriptional control of brown fat determination by PRDM16.", "journal": "Cell Metab.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "authors": "Yang W., Spiegelman B.M., Chin S., Kajimura S., Langin D., Rohas L.M., Uldry M., Tavernier G., Seale P.", "date": "2007" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/18483224", "type": [ "PubMed" ], "id": "18483224" } ], "title": "Regulation of the brown and white fat gene programs through a PRDM16/CtBP transcriptional complex.", "journal": "Genes Dev.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "authors": "Erdjument-Bromage H., Lazar M.A., Tempst P., Spiegelman B.M., Chin S., Kajimura S., Tomaru T., Ruas J.L., Cooper M.P., Seale P.", "date": "2008" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/18719582", "type": [ "PubMed" ], "id": "18719582" } ], "title": "PRDM16 controls a brown fat/skeletal muscle switch.", "journal": "Nature", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "authors": "Yang W., Erdjument-Bromage H., Tempst P., Spiegelman B.M., Beier D.R., Chin S., Kajimura S., Devarakonda S., Rudnicki M.A., Seale P., Scime A., Kuang S., Bjork B., Conroe H.M.", "date": "2008" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/19641492", "type": [ "PubMed" ], "id": "19641492" } ], "title": "Initiation of myoblast to brown fat switch by a PRDM16-C/EBP-beta transcriptional complex.", "journal": "Nature", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "authors": "Gygi S.P., Kubota K., Spiegelman B.M., Kajimura S., Frangioni J.V., Lunsford E., Seale P.", "date": "2009" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/22939622", "type": [ "PubMed" ], "id": "22939622" } ], "title": "Prdm3 and Prdm16 are H3K9me1 methyltransferases required for mammalian heterochromatin integrity.", "journal": "Cell", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "authors": "Reinberg D., Genoud C., Jenuwein T., Lachner M., Mittler G., Kurokawa M., Margueron R., Goyama S., Fritzsch C., Son J., Eisold M., Pinheiro I., Richter F.M., Shukeir N.", "date": "2012" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/23768516", "type": [ "PubMed" ], "id": "23768516" } ], "title": "Fine mapping of the 1p36 deletion syndrome identifies mutation of PRDM16 as a cause of cardiomyopathy.", "journal": "Am. J. Hum. Genet.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "authors": "Siebert R., Kalwa H., Caliebe A., Barton P.J., Klopocki E., Berger F., Klaassen S., Kramer H.H., Arndt A.K., Harnack C., Hubner N., MacRae C.A., Werdich A.A., Musso G., Greutmann M., Drenckhahn J.D., Bluhm J., Cook S.A., Heinig M., Martitz J., Padera R.F., Plovie E.R., Sabeh M.K., Schafer S., Wassilew K.", "date": "2013" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/25578880", "type": [ "PubMed" ], "id": "25578880" } ], "title": "Cold-inducible Zfp516 activates UCP1 transcription to promote browning of white fat and development of brown fat.", "journal": "Mol. Cell", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "authors": "Paul S.M., Kang C., Sul H.S., Dempersmier J., Gulyaeva O., Hudak C.S., Kwan H.Y., Raposo H.F., Sambeat A., Wong R.H.", "date": "2015" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/31564549", "type": [ "PubMed" ], "id": "31564549" } ], "title": "PRDM16 Maintains Homeostasis of the Intestinal Epithelium by Controlling Region-Specific Metabolism.", "journal": "Cell stem cell", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277955.1", "id": "NP_001277955.1", "database": "RefSeq" } ], "authors": "Stine RR, Sakers AP, TeSlaa T, Kissig M, Stine ZE, Kwon CW, Cheng L, Lim HW, Kaestner KH, Rabinowitz JD, Seale P", "date": "2019" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/32041791", "type": [ "PubMed" ], "id": "32041791" } ], "title": "Oxidative stress regulates progenitor behavior and cortical neurogenesis.", "journal": "Development (Cambridge, England)", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277955.1", "id": "NP_001277955.1", "database": "RefSeq" } ], "authors": "Chui A, Zhang Q, Dai Q, Shi SH", "date": "2020" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/32083975", "type": [ "PubMed" ], "id": "32083975" } ], "title": "Cardiac-specific inactivation of Prdm16 effects cardiac conduction abnormalities and cardiomyopathy-associated phenotypes.", "journal": "American journal of physiology. Heart and circulatory physiology", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277955.1", "id": "NP_001277955.1", "database": "RefSeq" } ], "authors": "Nam JM, Lim JE, Ha TW, Oh B, Kang JO", "date": "2020" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/32437910", "type": [ "PubMed" ], "id": "32437910" } ], "title": "Prdm3 and Prdm16 cooperatively maintain hematopoiesis and clonogenic potential.", "journal": "Experimental hematology", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277955.1", "id": "NP_001277955.1", "database": "RefSeq" } ], "authors": "McGlynn KA, Sun R, Vonica A, Rudzinskas S, Zhang Y, Perkins AS", "date": "2020" } ], "uniprot_ac": "A2A935", "site_annotation": [ { "end_pos": 1214, "annotation": "n_glycosylation_sequon", "start_pos": 1212 }, { "end_pos": 1260, "annotation": "n_glycosylation_sequon", "start_pos": 1258 }, { "end_pos": 567, "annotation": "n_glycosylation_sequon", "start_pos": 565 }, { "end_pos": 596, "annotation": "n_glycosylation_sequon", "start_pos": 594 }, { "end_pos": 611, "annotation": "n_glycosylation_sequon", "start_pos": 609 }, { "end_pos": 967, "annotation": "n_glycosylation_sequon", "start_pos": 965 } ], "isoforms": [ { "url": "https://www.uniprot.org/uniprot/A2A935#A2A929-1", "isoform_ac": "A2A929-1", "locus": { "end_pos": 154355278, "strand": "+", "start_pos": 154636759, "chromosome": "4", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000124771", "id": "ENSMUST00000124771", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000117598", "id": "ENSMUSP00000117598", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|A2A929-1|PRD16_MOUSE Computationally mapped isoform of Histone-lysine N-methyltransferase PRDM16 OS=Mus musculus OX=10090 GN=Prdm16 PE=1 SV=1 CANONICAL=A2A935-1", "length": 214, "sequence": "MRSKARARKLAKSDGDVVNNMYEPDPDLLAGQSAEEETEDGILSPIPMGPPSPFPTSEDFTPKEGSPYEAPVYIPEDIPIPPDFELRESSIPGAGLGIWAKRKMEIGERFGPYVVTPRAALKEADFGWEMLTDTEVSSQESCIKKISEDLGSEKFCVDANQAGSGSWLKYIRVACSCDDQNLAMCQINEQIYYKVIKDIEPGEELLVHVKEGAY" } }, { "url": "https://www.uniprot.org/uniprot/A2A935#A2A930-1", "isoform_ac": "A2A930-1", "locus": { "end_pos": 154320791, "strand": "+", "start_pos": 154636759, "chromosome": "4", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000097759", "id": "ENSMUST00000097759", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000095365", "id": "ENSMUSP00000095365", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|A2A930-1|PRD16_MOUSE Computationally mapped isoform of Histone-lysine N-methyltransferase PRDM16 OS=Mus musculus OX=10090 GN=Prdm16 PE=1 SV=1 CANONICAL=A2A935-1", "length": 1257, "sequence": "MRSKARARKLAKSDGDVVNNMYEPDPDLLAGQSAEEETEDGILSPIPMGPPSPFPTSEDFTPKEGSPYEAPVYIPEDIPIPPDFELRESSIPGAGLGIWAKRKMEIGERFGPYVVTPRAALKEADFGWEQMLTDTEVSSQESCIKKISEDLGSEKFCVDANQAGSGSWLKYIRVACSCDDQNLAMCQINEQIYYKVIKDIEPGEELLVHVKEGAYSLGVMAPSLDEDPTFRCDECDELFQCRLDLRRHKKYACSSAGAQLYEGLGEELKPEGLGVGSDGQAHECKDCERMFPNKYSLEQHMIVHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHSTVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHYTPGSIFTPGLPLTPSPMMDKTKPSPTLNHGGLGFSEYFPSRPHPGSLPFSAAPPAFPALTPGFPGIFPPSLYPRPPLLPPTPLLKSPLNHAQDAKLPSPLGNPALPLVSAVSNSSQGATAATGSEEKFDGRLEDAYAEKVKNRSPDMSDGSDFEDINTTTGTDLDTTTGTGSDLDSDLDSDRDKGKDKGKPVESKPEFGGASVPPGAMNSVAEVPAFYSQHSFFPPPEEQLLTASGAAGDSIKAIASIAEKYFGPGFMSMQEKKLGSLPYHSVFPFQFLPNFPHSLYPFTDRALAHNLLVKAEPKSPRDALKVGGPSAECPFDLTTKPKEAKPALLAPKVPLIPSSGEEQPLDLSIGSRARASQNGGGREPRKNHVYGERKPGVSEGLPKVCPAQLPQQPSLHYAKPSPFFMDPIYSRVEKRKVADPVGVLKEKYLRPSPLLFHPQMSAIETMTEKLESFAAMKADSGSSLQPLPHHPFNFRSPPPTLSDPILRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKHEHEGAPVSQHSGVLTNHLGTSASSPTSESDNHALLDEKEDSYFSEIRNFIANSEMNQASTRMDKRPEIQDLDSNPPCPGSASAKPEDVEEEEEEELEEEDDDSLAGKSQEDTVSPTPEPQGVYEDEEDEEPPSLTMGFDHTRRCVEERGGGLLALEPTPTFGKGLDLRRAAEEAFEVKDVLNSTLDSEVLKQTLYRQAKNQSSLDAWLNITGPSSESGAFNPINHL" } }, { "url": "https://www.uniprot.org/uniprot/A2A935#A2A931-1", "isoform_ac": "A2A931-1", "locus": { "end_pos": 154320791, "strand": "+", "start_pos": 154636759, "chromosome": "4", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000105637", "id": "ENSMUST00000105637", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000101262", "id": "ENSMUSP00000101262", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|A2A931-1|PRD16_MOUSE Computationally mapped isoform of Histone-lysine N-methyltransferase PRDM16 OS=Mus musculus OX=10090 GN=Prdm16 PE=1 SV=1 CANONICAL=A2A935-1", "length": 1274, "sequence": "MRSKARARKLAKSDGDVVNNMYEPDPDLLAGQSAEEETEDGILSPIPMGPPSPFPTSEDFTPKEGSPYEAPVYIPEDIPIPPDFELRESSIPGAGLGIWAKRKMEIGERFGPYVVTPRAALKEADFGWEMLTDTEVSSQESCIKKISEDLGSEKFCVDANQAGSGSWLKYIRVACSCDDQNLAMCQINEQIYYKVIKDIEPGEELLVHVKEGAYSLGVMAPSLDEDPTFRCDECDELFQCRLDLRRHKKYACSSAGAQLYEGLGEELKPEGLGVGSDGQAHECKDCERMFPNKYSLEQHMIVHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHSTVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHYTPGSIFTPGLPLTPSPMMDKTKPSPTLNHGGLGFSEYFPSRPHPGSLPFSAAPPAFPALTPGFPGIFPPSLYPRPPLLPPTPLLKSPLNHAQDAKLPSPLGNPALPLVSAVSNSSQGATAATGSEEKFDGRLEDAYAEKVKNRSPDMSDGSDFEDINTTTGTDLDTTTGTGSDLDSDLDSDRDKGKDKGKPVESKPEFGGASVPPGAMNSVAEVPAFYSQHSFFPPPEEQLLTASGAAGDSIKAIASIAEKYFGPGFMSMQEKKLGSLPYHSVFPFQFLPNFPHSLYPFTDRALAHNLLVKAEPKSPRDALKVGGPSAECPFDLTTKPKEAKPALLAPKVPLIPSSGEEQPLDLSIGSRARASQNGGGREPRKNHVYGERKPGVSEGLPKVCPAQLPQQPSLHYAKPSPFFMDPIYRVEKRKVADPVGVLKEKYLRPSPLLFHPQMSAIETMTEKLESFAAMKADSGSSLQPLPHHPFNFRSPPPTLSDPILRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKHEHEGAPVSQHSGVLTNHLGTSASSPTSESDNHALLDEKEDSYFSEIRNFIANSEMNQASTRMDKRPEIQDLDSNPPCPGSASAKPEDVEEEEEEELEEEDDDSLAGKSQEDTVSPTPEPQGVYEDEEDEEPPSLTMGFDHTRRCVEERGGGLLALEPTPTFGKGLDLRRAAEEAFEVKDVLNSTLDSEVLKQTLYRQAKNQAYAMMLSLSEDTPLHAPSQSSLDAWLNITGPSSESGAFNPINHL" } }, { "url": "https://www.uniprot.org/uniprot/A2A935#A2A933-1", "isoform_ac": "A2A933-1", "locus": { "end_pos": 154320791, "strand": "+", "start_pos": 154636759, "chromosome": "4", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000105638", "id": "ENSMUST00000105638", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000101263", "id": "ENSMUSP00000101263", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|A2A933-1|PRD16_MOUSE Computationally mapped isoform of Histone-lysine N-methyltransferase PRDM16 OS=Mus musculus OX=10090 GN=Prdm16 PE=1 SV=1 CANONICAL=A2A935-1", "length": 1276, "sequence": "MRSKARARKLAKSDGDVVNNMYEPDPDLLAGQSAEEETEDGILSPIPMGPPSPFPTSEDFTPKEGSPYEAPVYIPEDIPIPPDFELRESSIPGAGLGIWAKRKMEIGERFGPYVVTPRAALKEADFGWEQMLTDTEVSSQESCIKKISEDLGSEKFCVDANQAGSGSWLKYIRVACSCDDQNLAMCQINEQIYYKVIKDIEPGEELLVHVKEGAYSLGVMAPSLDEDPTFRCDECDELFQCRLDLRRHKKYACSSAGAQLYEGLGEELKPEGLGVGSDGQAHECKDCERMFPNKYSLEQHMIVHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHSTVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHYTPGSIFTPGLPLTPSPMMDKTKPSPTLNHGGLGFSEYFPSRPHPGSLPFSAAPPAFPALTPGFPGIFPPSLYPRPPLLPPTPLLKSPLNHAQDAKLPSPLGNPALPLVSAVSNSSQGATAATGSEEKFDGRLEDAYAEKVKNRSPDMSDGSDFEDINTTTGTDLDTTTGTGSDLDSDLDSDRDKGKDKGKPVESKPEFGGASVPPGAMNSVAEVPAFYSQHSFFPPPEEQLLTASGAAGDSIKAIASIAEKYFGPGFMSMQEKKLGSLPYHSVFPFQFLPNFPHSLYPFTDRALAHNLLVKAEPKSPRDALKVGGPSAECPFDLTTKPKEAKPALLAPKVPLIPSSGEEQPLDLSIGSRARASQNGGGREPRKNHVYGERKPGVSEGLPKVCPAQLPQQPSLHYAKPSPFFMDPIYSRVEKRKVADPVGVLKEKYLRPSPLLFHPQMSAIETMTEKLESFAAMKADSGSSLQPLPHHPFNFRSPPPTLSDPILRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKHEHEGAPVSQHSGVLTNHLGTSASSPTSESDNHALLDEKEDSYFSEIRNFIANSEMNQASTRMDKRPEIQDLDSNPPCPGSASAKPEDVEEEEEEELEEEDDDSLAGKSQEDTVSPTPEPQGVYEDEEDEEPPSLTMGFDHTRRCVEERGGGLLALEPTPTFGKGLDLRRAAEEAFEVKDVLNSTLDSEVLKQTLYRQAKNQAYAMMLSLSEDTPLHAPSQSSLDAWLNITGPSSESGAFNPINHL" } }, { "url": "https://www.uniprot.org/uniprot/A2A935#A2A934-1", "isoform_ac": "A2A934-1", "locus": { "end_pos": 154320913, "strand": "+", "start_pos": 154636759, "chromosome": "4", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000105636", "id": "ENSMUST00000105636", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000101261", "id": "ENSMUSP00000101261", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|A2A934-1|PRD16_MOUSE Computationally mapped isoform of Histone-lysine N-methyltransferase PRDM16 OS=Mus musculus OX=10090 GN=Prdm16 PE=1 SV=1 CANONICAL=A2A935-1", "length": 1177, "sequence": "MRSKARARKLAKSDGDVVNNMYEPDPDLLAGQSAEEETEDGILSPIPMGPPSPFPTSEDFTPKEGSPYEAPVYIPEDIPIPPDFELRESSIPGAGLGIWAKRKMEIGERFGPYVVTPRAALKEADFGWEQMLTDTEVSSQESCIKKQISEDLGSEKFCVDANQAGSGSWLKYIRVACSCDDQNLAMCQINEQIYYKVIKDIEPGEELLVHVKEGAYSLGVMAPSLDEDPTFRCDECDELFQCRLDLRRHKKYACSSAGAQLYEGLGEELKPEGLGVGSDGQAHECKDCERMFPNKYSLEQHMIVHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHSTVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHYTPGSIFTPGLPLTPSPMMDKTKPSPTLNHGGLGFSEYFPSRPHPGSLPFSAAPPAFPALTPGFPGIFPPSLYPRPPLLPPTPLLKSPLNHAQDAKLPSPLGNPALPLVSAVSNSSQGATAATGSEEKFDGRLEDAYAEKVKNRSPDMSDGSDFEDINTTTGTDLDTTTGTGSDLDSDLDSDRDKGKDKGKPVESKPEFGGASVPPGAMNSVAEVPAFYSQHSFFPPPEEQLLTASGAAGDSIKAIASIAEKYFGPGFMSMQEKKLGSLPYHSVFPFQFLPNFPHSLYPFTDRALAHNLLVKAEPKSPRDALKVGGPSAECPFDLTTKPKEAKPALLAPKVPLIPSSGEEQPLDLSIGSRARASQNGGGREPRKNHVYGERKPGVSEGLPKVCPAQLPQQPSLHYAKPSPFFMDPIYRVEKRKVADPVGVLKEKYLRPSPLLFHPQMSAIETMTEKLESFAAMKADSGSSLQPLPHHPFNFRSPPPTLSDPILRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKHEHEGAPVSQHSGVLTNHLGTSASSPTSESDNHALLDEKEDSYFSEIRNFIANSEMNQASTRMDKRPEIQDLDSNPPCPGSASAKPEDVEEEEEEELEEEDDDSLAGKSQEDTVSPTPEPQGVYEDEEDEEPPSLTMGFDHTRRHMQ" } }, { "url": "https://www.uniprot.org/uniprot/A2A935#A2A935-1", "isoform_ac": "A2A935-1", "locus": { "end_pos": 154320791, "strand": "+", "start_pos": 154636759, "chromosome": "4", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000030902", "id": "ENSMUST00000030902", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000030902", "id": "ENSMUSP00000030902", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "sp|A2A935-1|PRD16_MOUSE Isoform 1 of Histone-lysine N-methyltransferase PRDM16 OS=Mus musculus OX=10090 GN=Prdm16 PE=1 SV=1 CANONICAL=A2A935-1", "length": 1275, "sequence": "MRSKARARKLAKSDGDVVNNMYEPDPDLLAGQSAEEETEDGILSPIPMGPPSPFPTSEDFTPKEGSPYEAPVYIPEDIPIPPDFELRESSIPGAGLGIWAKRKMEIGERFGPYVVTPRAALKEADFGWEMLTDTEVSSQESCIKKQISEDLGSEKFCVDANQAGSGSWLKYIRVACSCDDQNLAMCQINEQIYYKVIKDIEPGEELLVHVKEGAYSLGVMAPSLDEDPTFRCDECDELFQCRLDLRRHKKYACSSAGAQLYEGLGEELKPEGLGVGSDGQAHECKDCERMFPNKYSLEQHMIVHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHSTVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHYTPGSIFTPGLPLTPSPMMDKTKPSPTLNHGGLGFSEYFPSRPHPGSLPFSAAPPAFPALTPGFPGIFPPSLYPRPPLLPPTPLLKSPLNHAQDAKLPSPLGNPALPLVSAVSNSSQGATAATGSEEKFDGRLEDAYAEKVKNRSPDMSDGSDFEDINTTTGTDLDTTTGTGSDLDSDLDSDRDKGKDKGKPVESKPEFGGASVPPGAMNSVAEVPAFYSQHSFFPPPEEQLLTASGAAGDSIKAIASIAEKYFGPGFMSMQEKKLGSLPYHSVFPFQFLPNFPHSLYPFTDRALAHNLLVKAEPKSPRDALKVGGPSAECPFDLTTKPKEAKPALLAPKVPLIPSSGEEQPLDLSIGSRARASQNGGGREPRKNHVYGERKPGVSEGLPKVCPAQLPQQPSLHYAKPSPFFMDPIYRVEKRKVADPVGVLKEKYLRPSPLLFHPQMSAIETMTEKLESFAAMKADSGSSLQPLPHHPFNFRSPPPTLSDPILRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKHEHEGAPVSQHSGVLTNHLGTSASSPTSESDNHALLDEKEDSYFSEIRNFIANSEMNQASTRMDKRPEIQDLDSNPPCPGSASAKPEDVEEEEEEELEEEDDDSLAGKSQEDTVSPTPEPQGVYEDEEDEEPPSLTMGFDHTRRCVEERGGGLLALEPTPTFGKGLDLRRAAEEAFEVKDVLNSTLDSEVLKQTLYRQAKNQAYAMMLSLSEDTPLHAPSQSSLDAWLNITGPSSESGAFNPINHL" } }, { "url": "https://www.uniprot.org/uniprot/A2A935#A2A935-2", "isoform_ac": "A2A935-2", "locus": { "end_pos": 154320913, "strand": "+", "start_pos": 154636759, "chromosome": "4", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000070313", "id": "ENSMUST00000070313", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000064546", "id": "ENSMUSP00000064546", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "sp|A2A935-2|PRD16_MOUSE Isoform 2 of Histone-lysine N-methyltransferase PRDM16 OS=Mus musculus OX=10090 GN=Prdm16 PE=1 SV=1 CANONICAL=A2A935-1", "length": 1178, "sequence": "MRSKARARKLAKSDGDVVNNMYEPDPDLLAGQSAEEETEDGILSPIPMGPPSPFPTSEDFTPKEGSPYEAPVYIPEDIPIPPDFELRESSIPGAGLGIWAKRKMEIGERFGPYVVTPRAALKEADFGWEQMLTDTEVSSQESCIKKQISEDLGSEKFCVDANQAGSGSWLKYIRVACSCDDQNLAMCQINEQIYYKVIKDIEPGEELLVHVKEGAYSLGVMAPSLDEDPTFRCDECDELFQCRLDLRRHKKYACSSAGAQLYEGLGEELKPEGLGVGSDGQAHECKDCERMFPNKYSLEQHMIVHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHSTVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHYTPGSIFTPGLPLTPSPMMDKTKPSPTLNHGGLGFSEYFPSRPHPGSLPFSAAPPAFPALTPGFPGIFPPSLYPRPPLLPPTPLLKSPLNHAQDAKLPSPLGNPALPLVSAVSNSSQGATAATGSEEKFDGRLEDAYAEKVKNRSPDMSDGSDFEDINTTTGTDLDTTTGTGSDLDSDLDSDRDKGKDKGKPVESKPEFGGASVPPGAMNSVAEVPAFYSQHSFFPPPEEQLLTASGAAGDSIKAIASIAEKYFGPGFMSMQEKKLGSLPYHSVFPFQFLPNFPHSLYPFTDRALAHNLLVKAEPKSPRDALKVGGPSAECPFDLTTKPKEAKPALLAPKVPLIPSSGEEQPLDLSIGSRARASQNGGGREPRKNHVYGERKPGVSEGLPKVCPAQLPQQPSLHYAKPSPFFMDPIYSRVEKRKVADPVGVLKEKYLRPSPLLFHPQMSAIETMTEKLESFAAMKADSGSSLQPLPHHPFNFRSPPPTLSDPILRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKHEHEGAPVSQHSGVLTNHLGTSASSPTSESDNHALLDEKEDSYFSEIRNFIANSEMNQASTRMDKRPEIQDLDSNPPCPGSASAKPEDVEEEEEEELEEEDDDSLAGKSQEDTVSPTPEPQGVYEDEEDEEPPSLTMGFDHTRRHMQ" } }, { "url": "https://www.uniprot.org/uniprot/A2A935#A2A935-3", "isoform_ac": "A2A935-3", "locus": {}, "sequence": { "header": "sp|A2A935-3|PRD16_MOUSE Isoform 3 of Histone-lysine N-methyltransferase PRDM16 OS=Mus musculus OX=10090 GN=Prdm16 PE=1 SV=1 CANONICAL=A2A935-1", "length": 1276, "sequence": "MRSKARARKLAKSDGDVVNNMYEPDPDLLAGQSAEEETEDGILSPIPMGPPSPFPTSEDFTPKEGSPYEAPVYIPEDIPIPPDFELRESSIPGAGLGIWAKRKMEIGERFGPYVVTPRAALKEADFGWEMLTDTEVSSQESCIKKQISEDLGSEKFCVDANQAGSGSWLKYIRVACSCDDQNLAMCQINEQIYYKVIKDIEPGEELLVHVKEGAYSLGVMAPSLDEDPTFRCDECDELFQCRLDLRRHKKYACSSAGAQLYEGLGEELKPEGLGVGSDGQAHECKDCERMFPNKYSLEQHMIVHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHSTVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHYTPGSIFTPGLPLTPSPMMDKTKPSPTLNHGGLGFSEYFPSRPHPGSLPFSAAPPAFPALTPGFPGIFPPSLYPRPPLLPPTPLLKSPLNHAQDAKLPSPLGNPALPLVSAVSNSSQGATAATGSEEKFDGRLEDAYAEKVKNRSPDMSDGSDFEDINTTTGTDLDTTTGTGSDLDSDLDSDRDKGKDKGKPVESKPEFGGASVPPGAMNSVAEVPAFYSQHSFFPPPEEQLLTASGAAGDSIKAIASIAEKYFGPGFMSMQEKKLGSLPYHSVFPFQFLPNFPHSLYPFTDRALAHNLLVKAEPKSPRDALKVGGPSAECPFDLTTKPKEAKPALLAPKVPLIPSSGEEQPLDLSIGSRARASQNGGGREPRKNHVYGERKPGVSEGLPKVCPAQLPQQPSLHYAKPSPFFMDPIYSRVEKRKVADPVGVLKEKYLRPSPLLFHPQMSAIETMTEKLESFAAMKADSGSSLQPLPHHPFNFRSPPPTLSDPILRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKHEHEGAPVSQHSGVLTNHLGTSASSPTSESDNHALLDEKEDSYFSEIRNFIANSEMNQASTRMDKRPEIQDLDSNPPCPGSASAKPEDVEEEEEEELEEEDDDSLAGKSQEDTVSPTPEPQGVYEDEEDEEPPSLTMGFDHTRRCVEERGGGLLALEPTPTFGKGLDLRRAAEEAFEVKDVLNSTLDSEVLKQTLYRQAKNQAYAMMLSLSEDTPLHAPSQSSLDAWLNITGPSSESGAFNPINHL" } } ], "pathway": [ { "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:70673", "resource": "KEGG Pathway", "id": "mmu:70673", "name": "" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-3214841", "resource": "Reactome", "id": "R-MMU-3214841", "name": "PKMTs methylate histone lysines" } ], "function": [ { "url": "https://www.uniprot.org/uniprot/A2A935#function", "annotation": "Binds DNA and functions as a transcriptional regulator (PubMed:18483224). Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (PubMed:22939622). Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation (PubMed:22939622). Likely to be one of the primary histone methyltransferases along with MECOM/PRDM3 that direct cytoplasmic H3K9me1 methylation (PubMed:22939622). Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism (PubMed:17618855, PubMed:18483224). Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells (PubMed:18719582, PubMed:19641492). Functions as a repressor of TGF-beta signaling.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935#function", "id": "A2A935", "database": "UniProtKB" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277955.1", "annotation": "Mice with Prdm16-null hearts develop abnormalities in cardiac conduction and cardiomyopathy-associated phenotypes, including fibrosis and cellular hypertrophy.", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277955.1", "id": "NP_001277955.1", "database": "RefSeq" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277955.1", "annotation": "Oxidative stress regulates progenitor behavior and cortical neurogenesis. Publication Status: Online-Only", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277955.1", "id": "NP_001277955.1", "database": "RefSeq" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277955.1", "annotation": "PRDM16 Maintains Homeostasis of the Intestinal Epithelium by Controlling Region-Specific Metabolism.", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277955.1", "id": "NP_001277955.1", "database": "RefSeq" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277955.1", "annotation": "PRDM16/MEL1 is a Smad binding protein that may be important for development of orofacial structures through modulation of the TGFbeta signaling pathway.", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277955.1", "id": "NP_001277955.1", "database": "RefSeq" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277955.1", "annotation": "Prdm3 and Prdm16 cooperatively maintain hematopoiesis and clonogenic potential.", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277955.1", "id": "NP_001277955.1", "database": "RefSeq" } ] } ], "orthologs": [ { "uniprot_canonical_ac": "A0A096MJ70-1", "protein_name": "PR/SET domain 16", "sequence": { "length": 1092, "sequence": "MCQINEQIYYKVIKDIEPGEELLVHVKEGAYSLGVMAPSLDEDPTFRCDECDELFQCRLDLRRHKKYACSSSGAPLYEGLGEELKPEGLGGGNDGQAHECKDCERMFPNKYSLEQHMIVHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHSTVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHYTPGGLFTPGLPLTPSPMMDKTKPSPTLNHGGLGFSEYFPSRPHPGSLPFSAAPPAFPTLTPGFPGIFPPSLYPRPPLLPPTPLLKSPLNHGQDAKLHSPLGNPALPLVSAVSNSSQGATAATGPEEKFDGRLEDSYVEKVKNRSPDMSDGSDFEDINTTTGTDLDTTTGTGSDLDSDLDSDRDKSKDKGKPAESKPEFGGASVPPGAMNSVAEVPAFYSQHSFFPPPEEQLLTASGAAGDSIKAIASIAEKYFGPGFMSMQEKKLGSLPYHSVFPFQFLPNFPHSLYPFTDRALAHNLLVKAEPKSPRDALKVGGPSAECPFDLTTKPKEAKPALLTPKAPLVPSSGEEQPLDLSIGSRARASQNGGSREPRKNHIYGERKPGVGEGLPKVCPAQLPQQPSLHYAKPSPFFMDPIYSRVEKRKVADPVGVLKEKYLRPSPLLFHPQMSAIETMTEKLESFAAMKADSGSSLQPLPHHPFNFRSPPPTLSDPILRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKHEHEGAPVSQHSGVLTNHLGTSASSPTSESDNHALLDEKEDSYFSEIRNFIANSEMNQASTRMDKRPEVQDLDGNPPCPGSASAKQEDVEEEEEEELEEEDDDSLAGKSQEDTVSPTPEPQGVYEDEEDEEPPNLTMDFDHTRRCVEERGGGLLALEPTPTFGKGLDLRRAAEEAFEVKDVLNSTLDSEVLKQTLYRQAKNQAYAMMLSLSEDTPLHAPSQSSLDAWLNITGPSSESGAFNPINHL" }, "evidence": [ { "url": "http://www.informatics.jax.org/homology/11139", "id": "11139", "database": "MGI" } ], "common_name": "Rat", "organism": "Rattus norvegicus", "gene_name": "Prdm16", "tax_id": 10116 }, { "uniprot_canonical_ac": "Q9HAZ2-1", "protein_name": "Histone-lysine N-methyltransferase PRDM16", "sequence": { "length": 1276, "sequence": "MRSKARARKLAKSDGDVVNNMYEPNRDLLASHSAEDEAEDSAMSPIPVGPPSPFPTSEDFTPKEGSPYEAPVYIPEDIPIPADFELRESSIPGAGLGVWAKRKMEAGERLGPCVVVPRAAAKETDFGWEQILTDVEVSPQEGCITKISEDLGSEKFCVDANQAGAGSWLKYIRVACSCDDQNLTMCQISEQIYYKVIKDIEPGEELLVHVKEGVYPLGTVPPGLDEEPTFRCDECDELFQSKLDLRRHKKYTCGSVGAALYEGLAEELKPEGLGGGSGQAHECKDCERMFPNKYSLEQHMVIHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHSTVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHYTPGGIFAPGLPLTPSPMMDKAKPSPSLNHASLGFNEYFPSRPHPGSLPFSTAPPTFPALTPGFPGIFPPSLYPRPPLLPPTSLLKSPLNHTQDAKLPSPLGNPALPLVSAVSNSSQGTTAAAGPEEKFESRLEDSCVEKLKTRSSDMSDGSDFEDVNTTTGTDLDTTTGTGSDLDSDVDSDPDKDKGKGKSAEGQPKFGGGLAPPGAPNSVAEVPVFYSQHSFFPPPDEQLLTATGAAGDSIKAIASIAEKYFGPGFMGMQEKKLGSLPYHSAFPFQFLPNFPHSLYPFTDRALAHNLLVKAEPKSPRDALKVGGPSAECPFDLTTKPKDVKPILPMPKGPSAPASGEEQPLDLSIGSRARASQNGGGREPRKNHVYGERKLGAGEGLPQVCPARMPQQPPLHYAKPSPFFMDPIYSRVEKRKVTDPVGALKEKYLRPSPLLFHPQMSAIETMTEKLESFAAMKADSGSSLQPLPHHPFNFRSPPPTLSDPILRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKHEHENAPVSQHPGVLTNHLGTSASSPTSESDNHALLDEKEDSYFSEIRNFIANSEMNQASTRTEKRADMQIVDGSAQCPGLASEKQEDVEEEDDDDLEEDDEDSLAGKSQDDTVSPAPEPQAAYEDEEDEEPAASLAVGFDHTRRCAEDHEGGLLALEPMPTFGKGLDLRRAAEEAFEVKDVLNSTLDSEALKHTLCRQAKNQAYAMMLSLSEDTPLHTPSQGSLDAWLKVTGATSESGAFHPINHL" }, "evidence": [ { "url": "http://www.informatics.jax.org/homology/11139", "id": "11139", "database": "MGI" } ], "common_name": "Human", "organism": "Homo sapiens", "gene_name": "PRDM16", "tax_id": 9606 } ], "interactions": [], "synthesized_glycans": [], "snv": [], "cluster_types": [ { "name": "isoformset.uniprotkb", "label": "Isoforms" }, { "name": "homologset.mgi", "label": "MGI Homologs" } ], "refseq": { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277955.1", "ac": "NP_001277955.1", "name": "histone-lysine N-methyltransferase PRDM16 isoform 3", "summary": "" }, "go_annotation": { "categories": [ { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0003713", "pmid": "18719582", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "name": "transcription coactivator activity", "id": "GO:0003713" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0003713", "pmid": "17618855", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "name": "transcription coactivator activity", "id": "GO:0003713" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0046332", "pmid": "17467076", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "name": "SMAD binding", "id": "GO:0046332" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0000978", "pmid": "21873635", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "name": "RNA polymerase II cis-regulatory region sequence-specific DNA binding", "id": "GO:0000978" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0043565", "pmid": "17618855", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "name": "sequence-specific DNA binding", "id": "GO:0043565" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0001228", "pmid": "21873635", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "name": "DNA-binding transcription activator activity, RNA polymerase II-specific", "id": "GO:0001228" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0003712", "pmid": "21873635", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "name": "transcription coregulator activity", "id": "GO:0003712" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0046974", "pmid": "22939622", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "name": "histone methyltransferase activity (H3-K9 specific)", "id": "GO:0046974" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0046872", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "name": "metal ion binding", "id": "GO:0046872" }, { "url": 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"https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "name": "white fat cell differentiation", "id": "GO:0050872" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0050873", "pmid": "18719582", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "name": "brown fat cell differentiation", "id": "GO:0050873" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0050873", "pmid": "19641492", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "name": "brown fat cell differentiation", "id": "GO:0050873" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0050873", "pmid": "18483224", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "name": "brown fat cell differentiation", "id": "GO:0050873" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0045892", "pmid": "18483224", "evidence": [ { 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"https://www.ebi.ac.uk/QuickGO/term/GO:0060021", "pmid": "20007998", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "name": "roof of mouth development", "id": "GO:0060021" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0030512", "pmid": "20007998", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "name": "negative regulation of transforming growth factor beta receptor signaling pathway", "id": "GO:0030512" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0022008", "pmid": "19050759", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "name": "neurogenesis", "id": "GO:0022008" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0035019", "pmid": "19379700", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "name": "somatic stem cell population 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"resource": "RefSeq", "name": "5730557K01Rik; cs; csp1; Mel; mel1" }, { "url": "https://www.uniprot.org/uniprot/A2A935", "type": "synonym", "resource": "UniProtKB", "name": "Kiaa1675" }, { "url": "https://www.uniprot.org/uniprot/A2A935", "type": "synonym", "resource": "UniProtKB", "name": "Mel1" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/A2A935", "type": "synonym", "resource": "RefSeq", "name": "Prdm16" }, { "url": "https://www.uniprot.org/uniprot/A2A935", "type": "recommended", "resource": "UniProtKB", "name": "Prdm16" } ], "pro_annotation": [], "crossref": [ { "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000039410", "id": "ENSMUSG00000039410", "database": "Bgee" }, { "url": "https://www.ncbi.nlm.nih.gov/gene/70673", "id": "70673", "database": "GeneID" }, { "url": "https://www.ebi.ac.uk/intact/interactors/id:A2A935*", "id": "A2A935", "database": "IntAct" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR001214", "id": "IPR001214", "database": "InterPro" }, { "url": 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"NP_001277955.1", "database": "RefSeq" }, { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" } ], "expression_disease": [], "glycosylation": [], "mutagenesis": [ { "sequence_org": "DL", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" }, { "url": "https://www.ncbi.nlm.nih.gov/pubmed/18483224", "id": "18483224", "database": "PubMed" } ], "sequence_mut": "AS", "start_pos": 805, "end_pos": 806, "annotation": "Loss of interaction with CTBP1 and CTBP2 and loss of repression of WAT-specific genes." }, { "sequence_org": "R", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "id": "A2A935", "database": "UniProtKB" }, { "url": "https://www.ncbi.nlm.nih.gov/pubmed/17618855", "id": "17618855", "database": "PubMed" } ], "sequence_mut": "Q", "start_pos": 996, "end_pos": 996, "annotation": "Loss of DNA-binding activity but no effect on PRDM16-mediated BAT gene transcription activation." } ], "disease": [], "phosphorylation": [], "mass": { "monoisotopic_mass": 140858.0, "chemical_mass": 140858.0 }, "expression_tissue": [], "gene": [ { "url": "https://www.uniprot.org/uniprot/A2A935", "name": "Prdm16", "locus": { "end_pos": 154316125, "strand": "-", "start_pos": 154636873, "chromosome": "4", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUSG00000039410", "id": "ENSMUSG00000039410", "database": "Ensembl Gene" } ] } } ], "protein_names": [ { "url": "https://www.uniprot.org/uniprot/A2A935#names_and_taxonomy", "type": "synonym", "resource": "UniProtKB", "name": "2.1.1.-" }, { "url": "https://www.uniprot.org/uniprot/A2A935#names_and_taxonomy", "type": "recommended", "resource": "UniProtKB", "name": "Histone-lysine N-methyltransferase PRDM16" }, { "url": "https://www.uniprot.org/uniprot/A2A935#names_and_taxonomy", "type": "synonym", "resource": "UniProtKB", "name": "MDS1/EVI1-like gene 1" }, { "url": "https://www.uniprot.org/uniprot/A2A935#names_and_taxonomy", "type": "synonym", "resource": "UniProtKB", "name": "PR domain zinc finger protein 16" }, { "url": "https://www.uniprot.org/uniprot/A2A935#names_and_taxonomy", "type": "synonym", "resource": "UniProtKB", "name": "PR domain-containing protein 16" }, { "url": "https://www.uniprot.org/uniprot/A2A935#names_and_taxonomy", "type": "synonym", "resource": "UniProtKB", "name": "Transcription factor MEL1" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/A2A935", "type": "synonym", "resource": "RefSeq", "name": "histone-lysine N-methyltransferase PRDM16 isoform 3" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/A2A935", "type": "synonym", "resource": "RefSeq", "name": "transcription factor MEL1; MDS1/EVI1-like gene 1; PR domain-containing protein 16; PR domain zinc finger protein 16; line 27; histone-lysine N-methyltransferase PRDM16" } ] }