{ "uniprot_canonical_ac": "A4QPC6-1", "sequence": { "header": "sp|A4QPC6-1|BT2A2_MOUSE Isoform 1 of Butyrophilin subfamily 2 member A2 OS=Mus musculus OX=10090 GN=Btn2a2 PE=1 SV=1 CANONICAL=A4QPC6-1", "length": 514, "sequence": "MEPTTSLRSCPIASLLFFLVLSLFVLVSAQFTVIGPAEPILAMVGENTTLHCHLSPERNAEEMEVRWFRWRFFPAVLVYRGHQERPEEQMVAYRGRTTFMRTDISKGRVALIIHNVTAYDNGIYCCYFQEGRSYDQATMKLMVASLGSEPLIKMKTLEDGSILLECTSEGWYPEPRAVWRDPYDEVVPALEEEYTADREGLFTVTMTIIIRDCSVRNMTCSVNNTLLSQEVESVILIPESFVPSLPLWMVAVAVTLPVVMLILLTSGSICLVKKHRRKKSILSAEKEAEYEEKEAARQLQEELRWRRTLLHAADVVLDPDTAHPELFLSDDQRSVIRGSSRQSVPDNPERFDCRPCVLGRESFSSGKHYWEVEVENVMVWAIGVCRDSVERKGEALLVPQNGFWTLEMFGSQYRALSSPEKIIPLKERLHRIAVFLDCEGGDISFYNMRDRSHIYTCPPVTFTGPLRPFFRLGSDDSPLFICPAFTGAQGVTIPEGGLFLYKTRPISQSLVRKP" }, "uniprot_id": "BT2A2_MOUSE", "enzyme_annotation": [], "glycation": [], "keywords": [ "glycoprotein", "glycoprotein_predicted" ], "species": [ { "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6", "id": "A4QPC6", "database": "UniProtKB" } ], "name": "Mus musculus", "taxid": 10090 } ], "publication": [ { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/20208008", "type": "PUBMED", "id": "20208008" } ], "title": "BTN1A1, the mammary gland butyrophilin, and BTN2A2 are both inhibitors of T cell activation.", "journal": "J. Immunol.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6", "id": "A4QPC6", "database": "UniProtKB" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001276544.1", "id": "NP_001276544.1", "database": "RefSeq" } ], "authors": "Trowsdale J., Mather I.H., Rhodes D.A., Palmer D.B., Ammann J.U., Aw D., Knezevic B.R., Smith I.A.", "date": "2010" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/26809444", "type": "PUBMED", "id": "26809444" } ], "title": "Btn2a2, a T cell immunomodulatory molecule coregulated with MHC class II genes.", "journal": "The Journal of experimental medicine", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001276544.1", "id": "NP_001276544.1", "database": "RefSeq" } ], "authors": "Sarter K, Leimgruber E, Gobet F, Agrawal V, Dunand-Sauthier I, Barras E, Mastelic-Gavillet B, Kamath A, Fontannaz P, Guery L, Duraes Fdo V, Lippens C, Ravn U, Santiago-Raber ML, Magistrelli G, Fischer N, Siegrist CA, Hugues S, Reith W", "date": "2016" } ], "uniprot_ac": "A4QPC6", "site_annotation": [ { "end_pos": 49, "annotation": "n_glycosylation_sequon", "start_pos": 47 }, { "end_pos": 219, "annotation": "n_glycosylation_sequon", "start_pos": 217 }, { "end_pos": 117, "annotation": "n_glycosylation_sequon", "start_pos": 115 }, { "end_pos": 225, "annotation": "n_glycosylation_sequon", "start_pos": 223 } ], "mutation": [], "isoforms": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6#I7HIQ7-1", "isoform_ac": "I7HIQ7-1", "locus": { "end_pos": 23478401, "start_pos": 23487917, "chromosome": "13", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000041541", "id": "ENSMUST00000041541", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000048251", "id": "ENSMUSP00000048251", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|I7HIQ7-1|BT2A2_MOUSE Computationally mapped isoform of Butyrophilin subfamily 2 member A2 OS=Mus musculus OX=10090 GN=Btn2a2 PE=1 SV=1 CANONICAL=A4QPC6-1", "length": 417, "sequence": "MEPTTSLRSCPIASLLFFLVLSLFVLVSAQFTVIGPAEPILAMVGENTTLHCHLSPERNAEEMEVRWFRWRFFPAVLVYRGHQERPEEQMVAYRGRTTFMRTDISKGRVALIIHNVTAYDNGIYCCYFQEGRSYDQATMKLMVASLGSEPLIKMKTLEDGSILLECTSEGWYPEPRAVWRDPYDEVVPALEEEYTADREGLFTVTMTIIIRDCSVRNMTCSVNNTLLSQEVESVILIPESFVPSLPLWMVAVAVTLPVVMLILLTSGSICLVKKHRRKKSILSAEKEAEYEEKEAARQLQEELRWRRTLLHAADVVLDPDTAHPELFLSDDQRSVIRGSSRQSVPDNPERFDCRPCVLGRESFSSGKHYWENGFWTLEMFGSQYRALSSPEKIIPLKERLHRIAVFLDYHTFTHVLL" } }, { "url": "https://www.uniprot.org/uniprot/A4QPC6#B9EJ60-1", "isoform_ac": "B9EJ60-1", "locus": { "end_pos": 23478237, "start_pos": 23487917, "chromosome": "13", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000223877", "id": "ENSMUST00000223877", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000153680", "id": "ENSMUSP00000153680", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|B9EJ60-1|BT2A2_MOUSE Computationally mapped isoform of Butyrophilin subfamily 2 member A2 OS=Mus musculus OX=10090 GN=Btn2a2 PE=1 SV=1 CANONICAL=A4QPC6-1", "length": 505, "sequence": "MEPTTSLRSCPIASLLFFLVLSLFVLVSAQFTVIGPAEPILAMVGENTTLHCHLSPERNAEEMEVRWFRWRFFPAVLVYRGHQERPEEQMVAYRGRTTFMRTDISKGRVALIIHNVTAYDNGIYCCYFQEGRSYDQATMKLMVASLGSEPLIKMKTLEDGSILLECTSEGWYPEPRAVWRDPYDEVVPALEEEYTADREGLFTVTMTIIIRDCSVRNMTCSVNNTLLSQEVESVILIPESFVPSLPLWMVAVAVTLPVVMLILLTSGSICLVKKHRRKKSILSAEKEAEYEEKEAARQLQEELPDVVLDPDTAHPELFLSDDQRSVIRGSSRQSVPDNPERFDCRPCVLGRESFSSGKHYWEVEVENVMVWAIGVCRDSVERKGEALLVPQNGFWTLEMFGSQYRALSSPEKIIPLKERLHRIAVFLDCEGGDISFYNMRDRSHIYTCPPVTFTGPLRPFFRLGSDDSPLFICPAFTGAQGVTIPEGGLFLYKTRPISQSLVRKP" } }, { "url": "https://www.uniprot.org/uniprot/A4QPC6#A4QPC6-2", "isoform_ac": "A4QPC6-2", "locus": { "end_pos": 0, "start_pos": 0, "chromosome": "N/A", "evidence": [] }, "sequence": { "header": "sp|A4QPC6-2|BT2A2_MOUSE Isoform 2 of Butyrophilin subfamily 2 member A2 OS=Mus musculus OX=10090 GN=Btn2a2 PE=1 SV=1 CANONICAL=A4QPC6-1", "length": 472, "sequence": "MEPTTSLRSCPIASLLFFLVLSLFVLVSAQFTVIGPAEPILAMVGENTTLHCHLSPERNAEEMEVRWFRWRFFPAVLVYRGHQERPEEQMVAYRGRTTFMRTDISKGRVALIIHNVTAYDNGIYCCYFQEGRSYDQATMKLMVASLGSEPLIKMKTLEDGSILLECTSEGWYPEPRAVWRDPYDEVVPALEEEYTADREGLFTVTMTIIIRDCSVRNMTCSVNNTLLSQEVESVILIPESFVPSLPLWMVAVAVTLPVVMLILLTSGSICLVKKHRRKKSILSAEKEAEYEEKEAARQLQEELRWRRTLLHAADVVLDPDTAHPELFLSDDQRSVIRGSSRQSVPDNPERFDCRPCVLGRESFSSGKHYWENGFWTLEMFGSQYRALSSPEKDHTSERASSPYSCLPGLSHIYTCPPVTFTGPLRPFFRLGSDDSPLFICPAFTGAQGVTIPEGGLFLYKTRPISQSLVRKP" } }, { "url": "https://www.uniprot.org/uniprot/A4QPC6#A4QPC6-1", "isoform_ac": "A4QPC6-1", "locus": { "end_pos": 23478237, "start_pos": 23487917, "chromosome": "13", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000110432", "id": "ENSMUST00000110432", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000106062", "id": "ENSMUSP00000106062", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "sp|A4QPC6-1|BT2A2_MOUSE Isoform 1 of Butyrophilin subfamily 2 member A2 OS=Mus musculus OX=10090 GN=Btn2a2 PE=1 SV=1 CANONICAL=A4QPC6-1", "length": 514, "sequence": "MEPTTSLRSCPIASLLFFLVLSLFVLVSAQFTVIGPAEPILAMVGENTTLHCHLSPERNAEEMEVRWFRWRFFPAVLVYRGHQERPEEQMVAYRGRTTFMRTDISKGRVALIIHNVTAYDNGIYCCYFQEGRSYDQATMKLMVASLGSEPLIKMKTLEDGSILLECTSEGWYPEPRAVWRDPYDEVVPALEEEYTADREGLFTVTMTIIIRDCSVRNMTCSVNNTLLSQEVESVILIPESFVPSLPLWMVAVAVTLPVVMLILLTSGSICLVKKHRRKKSILSAEKEAEYEEKEAARQLQEELRWRRTLLHAADVVLDPDTAHPELFLSDDQRSVIRGSSRQSVPDNPERFDCRPCVLGRESFSSGKHYWEVEVENVMVWAIGVCRDSVERKGEALLVPQNGFWTLEMFGSQYRALSSPEKIIPLKERLHRIAVFLDCEGGDISFYNMRDRSHIYTCPPVTFTGPLRPFFRLGSDDSPLFICPAFTGAQGVTIPEGGLFLYKTRPISQSLVRKP" } } ], "pathway": [ { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-8851680", "resource": "Reactome", "id": "R-MMU-8851680", "name": "Butyrophilin (BTN) family interactions" }, { "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:238555", "resource": "KEGG Pathway", "id": "mmu:238555", "name": "" } ], "function": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001276544.1", "annotation": "Btn2a2(-/-) mice exhibited enhanced effector CD4(+) and CD8(+) T cell responses, impaired CD4(+) regulatory T cell induction, potentiated antitumor responses, and exacerbated experimental autoimmune encephalomyelitis.", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001276544.1", "id": "NP_001276544.1", "database": "RefSeq" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001276544.1", "annotation": "These data are consistent with a coinhibitory role for mouse Butyrophilins", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001276544.1", "id": "NP_001276544.1", "database": "RefSeq" } ] }, { "url": "https://www.uniprot.org/uniprot/A4QPC6#function", "annotation": "Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6#function", "id": "A4QPC6", "database": "UniProtKB" } ] } ], "orthologs": [], "interactions": [], "synthesized_glycans": [], "refseq": { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001276544.1", "ac": "NP_001276544.1", "name": "butyrophilin subfamily 2 member A2 isoform 1 precursor", "summary": "" }, "go_annotation": { "categories": [ { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005102", "pmid": "21873635", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6", "id": "A4QPC6", "database": "UniProtKB" } ], "name": "signaling receptor binding", "id": "GO:0005102" } ], "total": 1, "name": "Molecular Function" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0009986", "pmid": "23589618", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6", "id": "A4QPC6", "database": "UniProtKB" } ], "name": "cell surface", "id": "GO:0009986" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0009897", "pmid": "21873635", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6", "id": "A4QPC6", "database": "UniProtKB" } ], "name": "external side of plasma membrane", "id": "GO:0009897" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0016021", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6", "id": "A4QPC6", "database": "UniProtKB" } ], "name": "integral component of membrane", "id": "GO:0016021" } ], "total": 3, "name": "Cellular Component" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0014067", "pmid": "23589618", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6", "id": "A4QPC6", "database": "UniProtKB" } ], "name": "negative regulation of phosphatidylinositol 3-kinase signaling", "id": "GO:0014067" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0050852", "pmid": "21873635", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6", "id": "A4QPC6", "database": "UniProtKB" } ], "name": "T cell receptor signaling pathway", "id": "GO:0050852" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0050776", "pmid": "21873635", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6", "id": "A4QPC6", "database": "UniProtKB" } ], "name": "regulation of immune response", "id": "GO:0050776" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0070373", "pmid": "23589618", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6", "id": "A4QPC6", "database": "UniProtKB" } ], "name": "negative regulation of ERK1 and ERK2 cascade", "id": "GO:0070373" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:2000134", "pmid": "23589618", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6", "id": "A4QPC6", "database": "UniProtKB" } ], "name": "negative regulation of G1/S transition of mitotic cell cycle", "id": "GO:2000134" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0051898", "pmid": "23589618", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6", "id": "A4QPC6", "database": "UniProtKB" } ], "name": "negative regulation of protein kinase B signaling", "id": "GO:0051898" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0046007", "pmid": "20208008", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6", "id": "A4QPC6", "database": "UniProtKB" } ], "name": "negative regulation of activated T cell proliferation", "id": "GO:0046007" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0050860", "pmid": "23589618", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6", "id": "A4QPC6", "database": "UniProtKB" } ], "name": "negative regulation of T cell receptor signaling pathway", "id": "GO:0050860" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0045591", "pmid": "23589618", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6", "id": "A4QPC6", "database": "UniProtKB" } ], "name": "positive regulation of regulatory T cell differentiation", "id": "GO:0045591" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0031324", "pmid": "20208008", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6", "id": "A4QPC6", "database": "UniProtKB" } ], "name": "negative regulation of cellular metabolic process", "id": "GO:0031324" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0050710", "pmid": "20208008", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6", "id": "A4QPC6", "database": "UniProtKB" } ], "name": "negative regulation of cytokine secretion", "id": "GO:0050710" } ], "total": 11, "name": "Biological Process" } ] }, "gene_names": [ { "type": "recommended", "resource": "uniprotkb", "name": "Btn2a2" }, { "type": "synonym", "resource": "refseq", "name": "Btn2; D030063K05" }, { "type": "synonym", "resource": "refseq", "name": "Btn2a2" } ], "crossref": [ { "url": "https://www.ebi.ac.uk/interpro/entry/IPR007110", "id": "IPR007110", "database": "InterPro" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR013106", "id": "IPR013106", "database": "InterPro" }, { "url": "http://research.bioinformatics.udel.edu/pro/entry/PR_A4QPC6", "id": "PR_A4QPC6", "database": "PRO" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR003879", "id": "IPR003879", "database": "InterPro" }, { "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:238555", "id": "mmu:238555", "database": "KEGG Pathway" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR013783", "id": "IPR013783", "database": "InterPro" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR006574", "id": "IPR006574", "database": "InterPro" }, { "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000053216", "id": "ENSMUSG00000053216", "database": "Bgee" }, { "url": "http://pfam.xfam.org/family/PF07686", "id": "PF07686", "database": "Pfam" }, { "url": "https://www.ncbi.nlm.nih.gov/gene/238555", "id": "238555", "database": "GeneID" }, { "url": "https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd15819", "id": "cd15819", "database": "CDD" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001276544.1", "id": "NP_001276544.1", "database": "RefSeq" }, { "url": "http://pfam.xfam.org/family/PF00622", "id": "PF00622", "database": "Pfam" }, { "url": "http://pfam.xfam.org/family/PF13765", "id": "PF13765", "database": "Pfam" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR003877", "id": "IPR003877", "database": "InterPro" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-8851680", "id": "R-MMU-8851680", "database": "Reactome" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR037958", "id": "IPR037958", "database": "InterPro" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR003599", "id": "IPR003599", "database": "InterPro" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR013320", "id": "IPR013320", "database": "InterPro" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR001870", "id": "IPR001870", "database": "InterPro" }, { "url": "http://www.informatics.jax.org/marker/MGI:3606486", "id": "3606486", "database": "MGI" }, { "url": "http://www.orthodb.org/?query=522383at2759", "id": "522383at2759", "database": "OrthoDB" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR036179", "id": "IPR036179", "database": "InterPro" } ], "expression_disease": [], "glycosylation": [ { "residue": "Asn", "site_category": "predicted", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6#ptm_processing", "id": "A4QPC6", "database": "UniProtKB" } ], "relation": "attached", "position": 115, "glytoucan_ac": "", "type": "N-linked" }, { "residue": "Asn", "site_category": "predicted", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6#ptm_processing", "id": "A4QPC6", "database": "UniProtKB" } ], "relation": "attached", "position": 47, "glytoucan_ac": "", "type": "N-linked" } ], "disease": [], "phosphorylation": [], "mass": { "monoisotopic_mass": 58455.0, "chemical_mass": 58455.0 }, "expression_tissue": [], "gene": [ { "url": "https://www.uniprot.org/uniprot/A4QPC6", "name": "Btn2a2", "locus": { "end_pos": 23477676, "start_pos": 23488857, "chromosome": "13", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUSG00000053216", "id": "ENSMUSG00000053216", "database": "Ensembl Gene" } ] } } ], "protein_names": [ { "type": "synonym", "resource": "refseq", "name": "butyrophilin subfamily 2 member A2 isoform 1 precursor" }, { "type": "synonym", "resource": "refseq", "name": "butyrophilin 2" }, { "type": "recommended", "resource": "uniprotkb", "name": "Butyrophilin subfamily 2 member A2" } ] }