{ "uniprot_canonical_ac": "A2APC3-1", "sequence": { "header": "sp|A2APC3-1|TTLL9_MOUSE Isoform 1 of Probable tubulin polyglutamylase TTLL9 OS=Mus musculus OX=10090 GN=Ttll9 PE=2 SV=2 CANONICAL=A2APC3-1", "length": 461, "sequence": "MSRQKNQNSKGHGVSKGKEREQRTLIRFKTTLMNTLMDVLRHRPGWVEVKDEGEWDFYWCDVSWLRENFDHTYMDEHVRISHFRNHYELTRKNYMVKNLKRFRKYLERESGKTEAAKCDFFPKTFEMPCEYHLFVEEFRKNPGITWIMKPVARSQGKGIFLFRRLKDIMDWRKGTSGKKPTGVETQPARANMNPSGSHDTRSSDDQKDDLPVENYVAQRYVENPYLIGGRKFDLRVYVLVMSYIPLRAWLYRDGFARFSNTRFTLNSIDDHYVHLTNVAVQKTSPDYHLKKGCKWMLQRFRQYLASKHGPKAVETLFSDMDNIFIKSLQSVQKVIISDKHCFELYGYDILIDQDLKPWLLEVNASPSLTASSQEDYELKTCLLEDTLHVVDMEARLTGKEKRVGGFDLMWNDGPVSREDGPSDLSGMGNFVTNTHLGCVNDRKEQLRQLFRSLQAQRKAPS" }, "uniprot_id": "TTLL9_MOUSE", "enzyme_annotation": [ { "ec": "6.-.-.-", "activity": "" } ], "glycation": [], "keywords": [], "species": [ { "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "id": "A2APC3", "database": "UniProtKB" } ], "name": "Mus musculus", "taxid": 10090 } ], "publication": [ { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/17499049", "type": "PUBMED", "id": "17499049" } ], "title": "A targeted multienzyme mechanism for selective microtubule polyglutamylation.", "journal": "Mol. Cell", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "id": "A2APC3", "database": "UniProtKB" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077087.1", "id": "NP_001077087.1", "database": "RefSeq" } ], "authors": "Janke C., Lacroix B., Rogowski K., van Dijk J., Miro J., Edde B.", "date": "2007" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/27257088", "type": "PUBMED", "id": "27257088" } ], "title": "Ttll9-/- mice sperm flagella show shortening of doublet 7, reduction of doublet 5 polyglutamylation and a stall in beating.", "journal": "J. Cell Sci.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "id": "A2APC3", "database": "UniProtKB" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077087.1", "id": "NP_001077087.1", "database": "RefSeq" } ], "authors": "Abe M., Yamazaki M., Shiba K., Setou M., Yao I., Ikegami K., Yang H.J., Konishi Y., Inaba K., Sakimura K., Konno A.", "date": "2016" }, { "reference": [ { "url": "https://www.ncbi.nlm.nih.gov/pubmed/29593216", "type": "PUBMED", "id": "29593216" } ], "title": "Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.", "journal": "Nature communications", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077087.1", "id": "NP_001077087.1", "database": "RefSeq" } ], "authors": "Ye B, Liu B, Hao L, Zhu X, Yang L, Wang S, Xia P, Du Y, Meng S, Huang G, Qin X, Wang Y, Yan X, Li C, Hao J, Zhu P, He L, Tian Y, Fan Z", "date": "2018" } ], "uniprot_ac": "A2APC3", "site_annotation": [ { "end_pos": 365, "annotation": "n_glycosylation_sequon", "start_pos": 363 } ], "mutation": [], "isoforms": [ { "url": "https://www.uniprot.org/uniprot/A2APC3#Z4YLM0-1", "isoform_ac": "Z4YLM0-1", "locus": { "end_pos": 152973579, "start_pos": 152984321, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000155631", "id": "ENSMUST00000155631", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000114786", "id": "ENSMUSP00000114786", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|Z4YLM0-1|TTLL9_MOUSE Computationally mapped isoform of Probable tubulin polyglutamylase TTLL9 OS=Mus musculus OX=10090 GN=Ttll9 PE=2 SV=1 CANONICAL=A2APC3-1", "length": 139, "sequence": "MTCDPRGSKEQRTLIRFKTTLMNTLMDVLRHRPGWVEVKDEGEWDFYWCDVSWLRENFDHTYMDEHVRISHFRNHYELTRKNYMVKNLKRFRKYLERESGKTEAAKCDFFPKTFEMPCEYHLFVEEFRKNPGITWIMKP" } }, { "url": "https://www.uniprot.org/uniprot/A2APC3#A2APC3-1", "isoform_ac": "A2APC3-1", "locus": { "end_pos": 152962541, "start_pos": 153006982, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000099197", "id": "ENSMUST00000099197", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000096803", "id": "ENSMUSP00000096803", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "sp|A2APC3-1|TTLL9_MOUSE Isoform 1 of Probable tubulin polyglutamylase TTLL9 OS=Mus musculus OX=10090 GN=Ttll9 PE=2 SV=2 CANONICAL=A2APC3-1", "length": 461, "sequence": "MSRQKNQNSKGHGVSKGKEREQRTLIRFKTTLMNTLMDVLRHRPGWVEVKDEGEWDFYWCDVSWLRENFDHTYMDEHVRISHFRNHYELTRKNYMVKNLKRFRKYLERESGKTEAAKCDFFPKTFEMPCEYHLFVEEFRKNPGITWIMKPVARSQGKGIFLFRRLKDIMDWRKGTSGKKPTGVETQPARANMNPSGSHDTRSSDDQKDDLPVENYVAQRYVENPYLIGGRKFDLRVYVLVMSYIPLRAWLYRDGFARFSNTRFTLNSIDDHYVHLTNVAVQKTSPDYHLKKGCKWMLQRFRQYLASKHGPKAVETLFSDMDNIFIKSLQSVQKVIISDKHCFELYGYDILIDQDLKPWLLEVNASPSLTASSQEDYELKTCLLEDTLHVVDMEARLTGKEKRVGGFDLMWNDGPVSREDGPSDLSGMGNFVTNTHLGCVNDRKEQLRQLFRSLQAQRKAPS" } }, { "url": "https://www.uniprot.org/uniprot/A2APC3#A2APC3-2", "isoform_ac": "A2APC3-2", "locus": { "end_pos": 152962541, "start_pos": 153003105, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000103155", "id": "ENSMUST00000103155", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000099444", "id": "ENSMUSP00000099444", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "sp|A2APC3-2|TTLL9_MOUSE Isoform 2 of Probable tubulin polyglutamylase TTLL9 OS=Mus musculus OX=10090 GN=Ttll9 PE=2 SV=2 CANONICAL=A2APC3-1", "length": 464, "sequence": "MSRQKNQNSKGHGVSKGKEREQRTLIRFKTTLMNTLMDVLRHRPGWVEVKDEGEWDFYWCDVSWLRENFDHTYMDEHVRISHFRNHYELTRKNYMVKNLKRFRKYLERESGKTEAAKCDFFPKTFEMPCEYHLFVEEFRKNPGITWIMKPVARSQGKGIFLFRRLKDIMDWRKGTSGKKPTGVETQPARANMNPSGSHDTRSSDDQKDDLPVENYVAQRYVENPYLIGGRKFDLRVYVLVMSYIPLRAWLYRDGFARFSNTRFTLNSIDDHYVHLTNVAVQKTSPDYHLKKGCKWMLQRFRQYLASKHGPKAVETLFSDMDNIFIKSLQSVQKVIISDKHCFELYGYDILIDQDLKPWLLEVNASPSLTASSQEDYELKTCLLEDTLHVVDMEARLTGKEKRVGGFDLMWNDGPVSREDGPSDLSGMGNFVTNTHLGTLSQTGSEWERNIIGSGGGLGPLGRAV" } }, { "url": "https://www.uniprot.org/uniprot/A2APC3#E9PUG4-1", "isoform_ac": "E9PUG4-1", "locus": { "end_pos": 152962541, "start_pos": 152999349, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000109801", "id": "ENSMUST00000109801", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000105426", "id": "ENSMUSP00000105426", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|E9PUG4-1|TTLL9_MOUSE Computationally mapped isoform of Probable tubulin polyglutamylase TTLL9 OS=Mus musculus OX=10090 GN=Ttll9 PE=2 SV=1 CANONICAL=A2APC3-1", "length": 234, "sequence": "MSRQKNQNSKGHGVSKGKEREQRTLIRFKTTLMNTLMDVLRHRPGWVEVKDEGEWDFYWCDVSWLRENFDHTYMDEHVRISHFRNHYELTRKNYMVKNLKRFRKYLERESGKTEAAKCDFFPKTFEMPCEYHLFVEEFRKNPGITWIMKPVARSQGKGIFLFRRLKDIMDWRKDTRSSDDQKDDLPVENYVAQRYVENPYLIGGRKFDLRVYVLVMSMFTSPMLLCRRHRLTTT" } }, { "url": "https://www.uniprot.org/uniprot/A2APC3#D6RCJ6-1", "isoform_ac": "D6RCJ6-1", "locus": { "end_pos": 152962541, "start_pos": 152976243, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000146626", "id": "ENSMUST00000146626", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000121434", "id": "ENSMUSP00000121434", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|D6RCJ6-1|TTLL9_MOUSE Computationally mapped isoform of Probable tubulin polyglutamylase TTLL9 OS=Mus musculus OX=10090 GN=Ttll9 PE=2 SV=1 CANONICAL=A2APC3-1", "length": 36, "sequence": "MSRQKRAENLNPLQDHPHEHTHGCSAPQARMGGSQR" } }, { "url": "https://www.uniprot.org/uniprot/A2APC3#D6RG93-1", "isoform_ac": "D6RG93-1", "locus": { "end_pos": 152962541, "start_pos": 152984194, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000152158", "id": "ENSMUST00000152158", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000122494", "id": "ENSMUSP00000122494", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|D6RG93-1|TTLL9_MOUSE Computationally mapped isoform of Probable tubulin polyglutamylase TTLL9 OS=Mus musculus OX=10090 GN=Ttll9 PE=2 SV=1 CANONICAL=A2APC3-1", "length": 126, "sequence": "MSRQKNQNSKGHGVSKGKEREQRTLIRFKTTLMNTLMDVLRHRPGWVEVKDEGEWDFYWCDVSWLRENFDHTYMDEHVRISHFRNHYEKWARQGTEGSPSALPCCLAYQEKLHGKEPEALPEVPGA" } }, { "url": "https://www.uniprot.org/uniprot/A2APC3#F6ZRM1-1", "isoform_ac": "F6ZRM1-1", "locus": { "end_pos": 152972288, "start_pos": 152991659, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000165343", "id": "ENSMUST00000165343", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000131839", "id": "ENSMUSP00000131839", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|F6ZRM1-1|TTLL9_MOUSE Computationally mapped isoform of Probable tubulin polyglutamylase TTLL9 OS=Mus musculus OX=10090 GN=Ttll9 PE=2 SV=1 CANONICAL=A2APC3-1", "length": 73, "sequence": "NQNSKGHGVSKGKEREQRTLIRFKTTLMNTLMDVLRHRPGWVEVKETQGVLMTRKMTSRWRTTWLSVTWKTPT" } } ], "pathway": [ { "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:74711", "resource": "KEGG Pathway", "id": "mmu:74711", "name": "" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-8955332", "resource": "Reactome", "id": "R-MMU-8955332", "name": "Carboxyterminal post-translational modifications of tubulin" } ], "function": [ { "url": "https://www.uniprot.org/uniprot/A2APC3#function", "annotation": "Probable tubulin polyglutamylase that forms polyglutamate side chains on tubulin (PubMed:27257088). Acts when complexed with other proteins (Probable). By mediating tubulin polyglutamylation, plays a role in the establishment of microtubule heterogeneity in sperm flagella (PubMed:27257088).", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3#function", "id": "A2APC3", "database": "UniProtKB" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077087.1", "annotation": "Ttll9 plays essential roles in maintaining the normal structure and beating pattern of sperm flagella by establishing normal heterogeneous polyglutamylation patterns.", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077087.1", "id": "NP_001077087.1", "database": "RefSeq" } ] } ], "orthologs": [], "interactions": [], "synthesized_glycans": [], "refseq": { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077087.1", "ac": "NP_001077087.1", "name": "probable tubulin polyglutamylase TTLL9 isoform 1", "summary": "" }, "go_annotation": { "categories": [ { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005524", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "id": "A2APC3", "database": "UniProtKB" } ], "name": "ATP binding", "id": "GO:0005524" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0016874", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "id": "A2APC3", "database": "UniProtKB" } ], "name": "ligase activity", "id": "GO:0016874" } ], "total": 2, "name": "Molecular Function" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005737", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "id": "A2APC3", "database": "UniProtKB" } ], "name": "cytoplasm", "id": "GO:0005737" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005874", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "id": "A2APC3", "database": "UniProtKB" } ], "name": "microtubule", "id": "GO:0005874" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005929", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "id": "A2APC3", "database": "UniProtKB" } ], "name": "cilium", "id": "GO:0005929" } ], "total": 3, "name": "Cellular Component" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0006464", "pmid": "", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "id": "A2APC3", "database": "UniProtKB" } ], "name": "cellular protein modification process", "id": "GO:0006464" } ], "total": 1, "name": "Biological Process" } ] }, "gene_names": [ { "type": "synonym", "resource": "refseq", "name": "Ttll9" }, { "type": "recommended", "resource": "uniprotkb", "name": "Ttll9" }, { "type": "synonym", "resource": "refseq", "name": "1700016F23Rik; 4930509O20Rik; AW046572" } ], "crossref": [ { "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR12241:SF39", "id": "PTHR12241:SF39", "database": "PANTHER" }, { "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:74711", "id": "mmu:74711", "database": "KEGG Pathway" }, { "url": "http://www.informatics.jax.org/marker/MGI:1913589", "id": "1913589", "database": "MGI" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR004344", "id": "IPR004344", "database": "InterPro" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR027751", "id": "IPR027751", "database": "InterPro" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077087.1", "id": "NP_001077087.1", "database": "RefSeq" }, { "url": "https://www.ncbi.nlm.nih.gov/gene/74711", "id": "74711", "database": "GeneID" }, { "url": "http://pfam.xfam.org/family/PF03133", "id": "PF03133", "database": "Pfam" }, { "url": "http://www.orthodb.org/?query=584228at2759", "id": "584228at2759", "database": "OrthoDB" }, { "url": "https://omabrowser.org/oma/group/YVPLKAW/", "id": "YVPLKAW", "database": "OMA" }, { "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000074673", "id": "ENSMUSG00000074673", "database": "Bgee" }, { "url": "http://research.bioinformatics.udel.edu/pro/entry/PR_A2APC3", "id": "PR_A2APC3", "database": "PRO" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-8955332", "id": "R-MMU-8955332", "database": "Reactome" } ], "expression_disease": [], "glycosylation": [], "disease": [], "phosphorylation": [], "mass": { "monoisotopic_mass": 54107.0, "chemical_mass": 54107.0 }, "expression_tissue": [], "gene": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "name": "Ttll9", "locus": { "end_pos": 153008482, "start_pos": 152962485, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUSG00000074673", "id": "ENSMUSG00000074673", "database": "Ensembl Gene" } ] } } ], "protein_names": [ { "type": "synonym", "resource": "uniprotkb", "name": "6.-.-.-" }, { "type": "synonym", "resource": "refseq", "name": "probable tubulin polyglutamylase TTLL9 isoform 1" }, { "type": "recommended", "resource": "uniprotkb", "name": "Probable tubulin polyglutamylase TTLL9" }, { "type": "synonym", "resource": "uniprotkb", "name": "Tubulin--tyrosine ligase-like protein 9" }, { "type": "synonym", "resource": "refseq", "name": "tubulin tyrosine ligase like family 9; probable tubulin polyglutamylase TTLL9; putative polyglutamylase; tubulin--tyrosine ligase-like protein 9" } ] }