{ "uniprot_canonical_ac": "A2RSX7-1", "sequence": { "length": 315, "sequence": "MAEQRLPVPRLRGVSREQFMEHLYPQRKPLVLEGLDLGSCTSKWTVDYLSQVGGTKEVKIHVAAVPQMDFISKNFVYRTLPFNKLVQRAAEETHKEFFISEDEKYYLRSLGEDPRKDVADIRQQFPSLGGDITFPMFFREEQFFSSVFRISSPGLQLWTHYDVMDNFLIQVTGKKRITLFNPRDAQYLYLSGSKSEVLNIDSPDLDKYPLFPKARRYECSLEAGDVLFIPALWFHNVVSEEFGVGVNIFWKHLPSECYDTTDTYGNKDPVAASRAVQILDRALKTLAELPEEYRDFYARQMVLRIQDKAYSKNFE" }, "uniprot_id": "TYW5_MOUSE", "enzyme_annotation": [ { "ec": "1.14.11.42", "activity": "7-(3-amino-3-carboxypropyl)wyosine(37) in tRNA(Phe) + 2-oxoglutarate + O(2) = 7-(2-hydroxy-3-amino-3-carboxypropyl)wyosine(37) in tRNA(Phe) + succinate + CO(2)." }, { "ec": "1.14.11.42", "activity": "" } ], "alternativenames": [ { "full": "tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-C(2))-hydroxylase", "short": "" } ], "keywords": [], "species": [ { "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2RSX7", "id": "A2RSX7", "database": "UniProtKB" } ], "name": "Mus musculus", "taxid": 10090 } ], "recommendedname": { "full": "tRNA wybutosine-synthesizing protein 5" }, "publication": [], "glycosylation": [], "mutation": [], "isoforms": [ { "url": "https://www.uniprot.org/uniprot/A2RSX7#A2RSX7-1", "isoform_ac": "A2RSX7-1", "locus": { "end_pos": 57388499, "start_pos": 57406461, "chromosome": "1", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000162686", "id": "ENSMUST00000162686", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000125427", "id": "ENSMUSP00000125427", "database": "Ensembl Peptide" } ] }, "sequence": { "length": 315, "sequence": "MAEQRLPVPRLRGVSREQFMEHLYPQRKPLVLEGLDLGSCTSKWTVDYLSQVGGTKEVKIHVAAVPQMDFISKNFVYRTLPFNKLVQRAAEETHKEFFISEDEKYYLRSLGEDPRKDVADIRQQFPSLGGDITFPMFFREEQFFSSVFRISSPGLQLWTHYDVMDNFLIQVTGKKRITLFNPRDAQYLYLSGSKSEVLNIDSPDLDKYPLFPKARRYECSLEAGDVLFIPALWFHNVVSEEFGVGVNIFWKHLPSECYDTTDTYGNKDPVAASRAVQILDRALKTLAELPEEYRDFYARQMVLRIQDKAYSKNFE" } }, { "url": "https://www.uniprot.org/uniprot/A2RSX7#E0CZH0-1", "isoform_ac": "E0CZH0-1", "locus": { "end_pos": 57393631, "start_pos": 57406461, "chromosome": "1", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000160118", "id": "ENSMUST00000160118", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000125386", "id": "ENSMUSP00000125386", "database": "Ensembl Peptide" } ] }, "sequence": { "length": 131, "sequence": "MAEQRLPVPRLRGVSREQFMEHLYPQRKPLVLEGLDLGSCTSKWTVDYLSQVGGTKEVKIHVAAVPQMDFIKLYLLTSWSREQPKKHIKNSSFQRMRNTTYGHLEKTQGSRKNSFTGKVGCCCWAFPIIGL" } }, { "url": "https://www.uniprot.org/uniprot/A2RSX7#A2RSX7-2", "isoform_ac": "A2RSX7-2", "locus": { "end_pos": 57393454, "start_pos": 57406461, "chromosome": "1", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000079998", "id": "ENSMUST00000079998", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000078912", "id": "ENSMUSP00000078912", "database": "Ensembl Peptide" } ] }, "sequence": { "length": 121, "sequence": "MAEQRLPVPRLRGVSREQFMEHLYPQRKPLVLEGLDLGSCTSKWTVDYLSQVGGTKEVKIHVAAVPQMDFIKLYLLTSWSREQPKKHIKNSSFQRMRNTTYGHLEKTQGRMLQTSDSSSHH" } }, { "url": "https://www.uniprot.org/uniprot/A2RSX7#G3UW53-1", "isoform_ac": "G3UW53-1", "locus": { "end_pos": 57388667, "start_pos": 57406443, "chromosome": "1", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000160837", "id": "ENSMUST00000160837", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000128576", "id": "ENSMUSP00000128576", "database": "Ensembl Peptide" } ] }, "sequence": { "length": 255, "sequence": "PVPRLRGVSREQFMEHLYPQRKPLVLEGLDLGSCTSKWTVDYLSQVGGTKEVKIHVAAVPQMDFIKLYLLTSWSREQPKKHIKNSSFQRMRNTTYGHLEKTQGSRKNSFTGKDVADIRQQFPSLGGDITFPMFFREEQFFSSVFRISSPGLQLWTHYDVMDNFLIQVTGKKRITLFNPRDAQYLYLSGSKSEVLNIDSPDLDKYPLFPKARRYECSLEAGDVLFIPALWFHNVVSEEFGVGVNIFWKHLPSECYD" } } ], "pathway": [ { "url": "https://www.genome.jp/dbget-bin/www_bget?mmu:68736", "resource": "KEGG Pathway", "id": "mmu:68736", "name": "" } ], "function": [ { "url": "https://www.uniprot.org/uniprot/A2RSX7#function", "annotation": "tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes (By similarity).", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2RSX7#function", "id": "A2RSX7", "database": "UniProtKB" } ] } ], "orthologs": [ { "uniprot_canonical_ac": "A2RUC4-1", "protein_name": "tRNA wybutosine-synthesizing protein 5", "sequence": { "length": 315, "sequence": "MAGQHLPVPRLEGVSREQFMQHLYPQRKPLVLEGIDLGPCTSKWTVDYLSQVGGKKEVKIHVAAVAQMDFISKNFVYRTLPFDQLVQRAAEEKHKEFFVSEDEKYYLRSLGEDPRKDVADIRKQFPLLKGDIKFPEFFKEEQFFSSVFRISSPGLQLWTHYDVMDNLLIQVTGKKRVVLFSPRDAQYLYLKGTKSEVLNIDNPDLAKYPLFSKARRYECSLEAGDVLFIPALWFHNVISEEFGVGVNIFWKHLPSECYDKTDTYGNKDPTAASRAAQILDRALKTLAELPEEYRDFYARRMVLHIQDKAYSKNSE" }, "evidence": [ { "url": "http://www.informatics.jax.org/homology/72085", "id": "72085", "database": "MGI" }, { "url": "https://omabrowser.org/oma/group/A2RSX7/", "id": "A2RSX7", "database": "OMA" } ], "organism": "Homo sapiens", "tax_id": 9606 }, { "uniprot_canonical_ac": "D3ZY75-1", "protein_name": "", "sequence": { "length": 315, "sequence": "MAEQRLPVPRLRGVSREQFMEHLYPQRKPLVLEGLDLGSCTSKWTVDYLSQVGGTKEVKIHVAAVAQMDFISKNFVYRTLPFNKLVQRAAEETHKEFFISQDERYYLRSLGEDPRKDIADIRQQFPSLGEDITFPMFFREEQFFSSVFRISSPGLQLWTHYDVMDNFLIQVTGKKRITLFSPRDAQYLYLSGSKSEVLNIDSPDLDKYPLFPKARRYECSLEAGDVLFIPALWFHNVVSEEFGVGVNVFLKHLPSECYDTTDTYGNKDPVAASRAMQILDRALKTLAELPEEYRDFYARQMVLRIQDKAYSKNSE" }, "evidence": [ { "url": "http://www.informatics.jax.org/homology/72085", "id": "72085", "database": "MGI" }, { "url": "https://omabrowser.org/oma/group/A2RSX7/", "id": "A2RSX7", "database": "OMA" } ], "organism": "Rattus norvegicus", "tax_id": 10116 } ], "refseq": { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001032831.2", "ac": "NP_001032831.2", "name": "tRNA wybutosine-synthesizing protein 5 isoform 1", "summary": "" }, "go_annotation": { "categories": [ { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005506", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2RSX7", "id": "A2RSX7", "database": "UniProtKB" } ], "name": "iron ion binding", "id": "GO:0005506" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0042803", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2RSX7", "id": "A2RSX7", "database": "UniProtKB" } ], "name": "protein homodimerization activity", "id": "GO:0042803" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0000049", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2RSX7", "id": "A2RSX7", "database": "UniProtKB" } ], "name": "tRNA binding", "id": "GO:0000049" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0016706", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2RSX7", "id": "A2RSX7", "database": "UniProtKB" } ], "name": "2-oxoglutarate-dependent dioxygenase activity", "id": "GO:0016706" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0102524", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2RSX7", "id": "A2RSX7", "database": "UniProtKB" } ], "name": "tRNAPhe (7-(3-amino-3-carboxypropyl)wyosine37-C2)-hydroxylase activity", "id": "GO:0102524" } ], "total": 5, "name": "Molecular Function" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0031591", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2RSX7", "id": "A2RSX7", "database": "UniProtKB" } ], "name": "wybutosine biosynthetic process", "id": "GO:0031591" } ], "total": 1, "name": "Biological Process" } ] }, "site_annotation": [], "crossref": [ { "url": "https://www.genome.jp/dbget-bin/www_bget?mmu:68736", "id": "mmu:68736", "database": "KEGG Pathway" }, { "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000048495", "id": "ENSMUSG00000048495", "database": "Bgee" }, { "url": "http://www.informatics.jax.org/marker/MGI:1915986", "id": "1915986", "database": "MGI" }, { "url": "https://omabrowser.org/oma/group/PLYDDRP/", "id": "PLYDDRP", "database": "OMA" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR003347", "id": "IPR003347", "database": "InterPro" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR029612", "id": "IPR029612", "database": "InterPro" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR041667", "id": "IPR041667", "database": "InterPro" }, { "url": "https://www.ncbi.nlm.nih.gov/gene/68736", "id": "68736", "database": "GeneID" }, { "url": "http://pfam.xfam.org/family/PF13621", "id": "PF13621", "database": "Pfam" }, { "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR12461:SF50", "id": "PTHR12461:SF50", "database": "PANTHER" }, { "url": "http://research.bioinformatics.udel.edu/pro/entry/PR_A2RSX7", "id": "PR_A2RSX7", "database": "PRO" } ], "expression_disease": [], "uniprot_ac": "A2RSX7", "disease": [], "mass": { "monoisotopic_mass": 36633.0, "chemical_mass": 36633.0 }, "expression_tissue": [], "gene": [ { "url": "http://www.informatics.jax.org/marker/MGI:1915986", "name": "Tyw5", "locus": { "end_pos": 57388237, "start_pos": 57407101, "chromosome": "1", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUSG00000048495", "id": "ENSMUSG00000048495", "database": "Ensembl Gene" } ] } } ] }