{ "uniprot_canonical_ac": "A2BDX3-1", "sequence": { "length": 460, "sequence": "MAAPEDVAALQAEITRREEELASLKRRLAAALTAEPEPERPLRVPPPPLAPRAALSRDEILRYSRQLLLPELGVRGQLRLAAAAVLVVGCGGLGCPLAQYLAAAGVGRLGLVDHDVVETSNLARQVLHGEAQAGESKARSAAAALRRLNSAVECVAYPRALAEDWALDLVRGYDVVADCCDNVPTRYLVNDACVLAGRPLVSASALRFEGQMTVYHHDGGPCYRCVFPRPPPPETVTNCADGGVLGAVPGVLGCAQALEVLKIAAGLGSSYSGSMLLFDGLGGHFRRIRLRRRRPDCVVCGQQPTVTRLQDYEAFCGSSATDKCRALKLLCPEERISVTDYKRLLDSGAPHVLLDVRPQVEVDICRLPHSLHIPLSQLERRDADSLKLLGAALRKGKQESQEGVALPVYVICKLGNDSQKAVKVLQSLTAVPELDSLTVQDIVGGLMAWAAKIDGTFPQY" }, "uniprot_id": "MOCS3_MOUSE", "enzyme_annotation": [], "alternativenames": [ { "full": "Molybdenum cofactor synthesis protein 3", "short": "" } ], "keywords": [], "species": [ { "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3", "id": "A2BDX3", "database": "UniProtKB" } ], "name": "Mus musculus", "taxid": 10090 } ], "recommendedname": { "full": "Adenylyltransferase and sulfurtransferase MOCS3" }, "publication": [], "glycosylation": [], "mutation": [], "isoforms": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3#A2BDX3-1", "isoform_ac": "A2BDX3-1", "locus": { "end_pos": 168230635, "start_pos": 168232014, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000099071", "id": "ENSMUST00000099071", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000096670", "id": "ENSMUSP00000096670", "database": "Ensembl Peptide" } ] }, "sequence": { "length": 460, "sequence": "MAAPEDVAALQAEITRREEELASLKRRLAAALTAEPEPERPLRVPPPPLAPRAALSRDEILRYSRQLLLPELGVRGQLRLAAAAVLVVGCGGLGCPLAQYLAAAGVGRLGLVDHDVVETSNLARQVLHGEAQAGESKARSAAAALRRLNSAVECVAYPRALAEDWALDLVRGYDVVADCCDNVPTRYLVNDACVLAGRPLVSASALRFEGQMTVYHHDGGPCYRCVFPRPPPPETVTNCADGGVLGAVPGVLGCAQALEVLKIAAGLGSSYSGSMLLFDGLGGHFRRIRLRRRRPDCVVCGQQPTVTRLQDYEAFCGSSATDKCRALKLLCPEERISVTDYKRLLDSGAPHVLLDVRPQVEVDICRLPHSLHIPLSQLERRDADSLKLLGAALRKGKQESQEGVALPVYVICKLGNDSQKAVKVLQSLTAVPELDSLTVQDIVGGLMAWAAKIDGTFPQY" } } ], "pathway": [ { "url": "https://www.genome.jp/dbget-bin/www_bget?mmu:69372", "resource": "KEGG Pathway", "id": "mmu:69372", "name": "" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-947581", "resource": "Reactome", "id": "R-MMU-947581", "name": "Molybdenum cofactor biosynthesis" } ], "function": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3#function", "annotation": "Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3#function", "id": "A2BDX3", "database": "UniProtKB" } ] } ], "orthologs": [ { "uniprot_canonical_ac": "D4A8L5-1", "protein_name": "Adenylyltransferase and sulfurtransferase MOCS3", "sequence": { "length": 458, "sequence": "MAAPEDVAALQDEIARREEELASLKQRLAAALAVEPEPERPIPVMPLPSRAALSRDEILRYSRQLLLPELGVRGQLRLAAASVLVVGCGGLGCPLAQYLAAAGVGRLGLVDHDVVETSNLARQVLHGEAQAGHAKAWSAAAALRRLNSAVEYVPYARALSEAWALDLVRGYDVVADCSDNVPTRYLVNDACVLAGRPLVSASALRFEGQMTVYHYDDGPCYRCVFPRPPPAETVTNCADGGVLGVVPGVLGCVQALEVLKIAAGLGTTYSGSMLLFDGLGGHFRRIRLRRRRPDCVVCGQQPTVTCLKNYEAFCGSSATDKCRSLKLLSPEERISVTDYKRLLDSGVPHVLLDVRPQVEVDICRLQHSLHIPLSLLERRDADSLKLLGAALQEEKRNSQEGAALAVYVICKLGNDSQKAVRVLQSLTAVPELDSLKVQDISGGLMAWAAKIDGTFPQY" }, "evidence": [ { "url": "https://omabrowser.org/oma/group/A2BDX3/", "id": "A2BDX3", "database": "OMA" }, { "url": "http://www.informatics.jax.org/homology/6108", "id": "6108", "database": "MGI" } ], "organism": "Rattus norvegicus", "tax_id": 10116 }, { "uniprot_canonical_ac": "O95396-1", "protein_name": "Adenylyltransferase and sulfurtransferase MOCS3", "sequence": { "length": 460, "sequence": "MASREEVLALQAEVAQREEELNSLKQKLASALLAEQEPQPERLVPVSPLPPKAALSRDEILRYSRQLVLPELGVHGQLRLGTACVLIVGCGGLGCPLAQYLAAAGVGRLGLVDYDVVEMSNLARQVLHGEALAGQAKAFSAAASLRRLNSAVECVPYTQALTPATALDLVRRYDVVADCSDNVPTRYLVNDACVLAGRPLVSASALRFEGQITVYHYDGGPCYRCIFPQPPPAETVTNCADGGVLGVVTGVLGCLQALEVLKIAAGLGPSYSGSLLLFDALRGHFRSIRLRSRRLDCAACGERPTVTDLLDYEAFCGSSATDKCRSLQLLSPEERVSVTDYKRLLDSGAFHLLLDVRPQVEVDICRLPHALHIPLKHLERRDAESLKLLKEAIWEEKQGTQEGAAVPIYVICKLGNDSQKAVKILQSLSAAQELDPLTVRDVVGGLMAWAAKIDGTFPQY" }, "evidence": [ { "url": "https://omabrowser.org/oma/group/A2BDX3/", "id": "A2BDX3", "database": "OMA" }, { "url": "http://www.informatics.jax.org/homology/6108", "id": "6108", "database": "MGI" } ], "organism": "Homo sapiens", "tax_id": 9606 } ], "refseq": { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001153802.1", "ac": "NP_001153802.1", "name": "adenylyltransferase and sulfurtransferase MOCS3", "summary": "" }, "go_annotation": { "categories": [ { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0016783", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3", "id": "A2BDX3", "database": "UniProtKB" } ], "name": "sulfurtransferase activity", "id": "GO:0016783" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005524", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3", "id": "A2BDX3", "database": "UniProtKB" } ], "name": "ATP binding", "id": "GO:0005524" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0046872", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3", "id": "A2BDX3", "database": "UniProtKB" } ], "name": "metal ion binding", "id": "GO:0046872" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0016779", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3", "id": "A2BDX3", "database": "UniProtKB" } ], "name": "nucleotidyltransferase activity", "id": "GO:0016779" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0004792", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3", "id": "A2BDX3", "database": "UniProtKB" } ], "name": "thiosulfate sulfurtransferase activity", "id": "GO:0004792" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0042292", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3", "id": "A2BDX3", "database": "UniProtKB" } ], "name": "URM1 activating enzyme activity", "id": "GO:0042292" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0061604", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3", "id": "A2BDX3", "database": "UniProtKB" } ], "name": "molybdopterin-synthase sulfurtransferase activity", "id": "GO:0061604" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0061605", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3", "id": "A2BDX3", "database": "UniProtKB" } ], "name": "molybdopterin-synthase adenylyltransferase activity", "id": "GO:0061605" } ], "total": 8, "name": "Molecular Function" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005737", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3", "id": "A2BDX3", "database": "UniProtKB" } ], "name": "cytoplasm", "id": "GO:0005737" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005829", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3", "id": "A2BDX3", "database": "UniProtKB" } ], "name": "cytosol", "id": "GO:0005829" } ], "total": 2, "name": "Cellular Component" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0002143", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3", "id": "A2BDX3", "database": "UniProtKB" } ], "name": "tRNA wobble position uridine thiolation", "id": "GO:0002143" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0034227", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3", "id": "A2BDX3", "database": "UniProtKB" } ], "name": "tRNA thio-modification", "id": "GO:0034227" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0002098", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3", "id": "A2BDX3", "database": "UniProtKB" } ], "name": "tRNA wobble uridine modification", "id": "GO:0002098" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0006777", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3", "id": "A2BDX3", "database": "UniProtKB" } ], "name": "Mo-molybdopterin cofactor biosynthetic process", "id": "GO:0006777" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0018192", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3", "id": "A2BDX3", "database": "UniProtKB" } ], "name": "enzyme active site formation via cysteine modification to L-cysteine persulfide", "id": "GO:0018192" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0032447", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2BDX3", "id": "A2BDX3", "database": "UniProtKB" } ], "name": "protein urmylation", "id": "GO:0032447" } ], "total": 6, "name": "Biological Process" } ] }, "site_annotation": [ { "end_pos": 418, "annotation": "n_glycosylation_sequon", "start_pos": 416 } ], "crossref": [ { "url": "https://www.genome.jp/dbget-bin/www_bget?mmu:69372", "id": "mmu:69372", "database": "KEGG Pathway" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-947581", "id": "R-MMU-947581", "database": "Reactome" }, { "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000074576", "id": "ENSMUSG00000074576", "database": "Bgee" }, { "url": "http://www.informatics.jax.org/marker/MGI:1916622", "id": "1916622", "database": "MGI" }, { "url": "https://omabrowser.org/oma/group/GTIGAMQ/", "id": "GTIGAMQ", "database": "OMA" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR000594", "id": "IPR000594", "database": "InterPro" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR035985", "id": "IPR035985", "database": "InterPro" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR001763", "id": "IPR001763", "database": "InterPro" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR036873", "id": "IPR036873", "database": "InterPro" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR028885", "id": "IPR028885", "database": "InterPro" }, { "url": "https://www.ncbi.nlm.nih.gov/gene/69372", "id": "69372", "database": "GeneID" }, { "url": "http://pfam.xfam.org/family/PF00899", "id": "PF00899", "database": "Pfam" }, { "url": "http://pfam.xfam.org/family/PF00581", "id": "PF00581", "database": "Pfam" }, { "url": "http://research.bioinformatics.udel.edu/pro/entry/PR_A2BDX3", "id": "PR_A2BDX3", "database": "PRO" }, { "url": "https://www.ebi.ac.uk/intact/interactors/id:A2BDX3*", "id": "A2BDX3", "database": "IntAct" } ], "expression_disease": [], "uniprot_ac": "A2BDX3", "disease": [], "mass": { "monoisotopic_mass": 49375.0, "chemical_mass": 49375.0 }, "expression_tissue": [], "gene": [ { "url": "http://www.informatics.jax.org/marker/MGI:1916622", "name": "Mocs3", "locus": { "end_pos": 168232594, "start_pos": 168230622, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUSG00000074576", "id": "ENSMUSG00000074576", "database": "Ensembl Gene" } ] } } ] }