{ "uniprot_canonical_ac": "A2RRT9-1", "sequence": { "header": "sp|A2RRT9-1|CP4V2_RAT Isoform 1 of Cytochrome P450 4V2 OS=Rattus norvegicus OX=10116 GN=Cyp4v2 PE=2 SV=1 CANONICAL=A2RRT9-1", "length": 525, "sequence": "MLWLWLGLSGQKLLLWGAASAVSVAGATVLLNILQMLVSYARKWQQMRPIPSVARAYPLVGHALFMKPNNTEFFQQIIQYTEEFRHLPIIKLWIGPVPLVALYKAENVEVILTSSKQIDKSFMYKFLQPWLGLGLLTSTGSKWRARRKMLTPSFHFTILEDFLDVMNEQANILVNKLEKHVNQEAFNCFFPITLCALDIICETAMGKNIGAQSNGDSEYVRTVYRMSDMIYRRMKMPWFWFDLWYLMFKEGRDHKKGLKSLHTFTNNVIAERVNARKAEQDCIGAGRGPLPSKTKRKAFLDLLLSVTDEEGNKLSHEDIREEVDTFMFEGHDTTAAAINWSLYLLGSNPEVQRKVDKELDDVFGRSHRPVTLEDLKKLKYLDCVIKETLRVFPSVPLFARSLSEDCEVAGYKISKGTEAVIIPYALHRDPRYFPDPEEFQPERFFPENSQGRHPYAYVPFSAGPRNCIGQKFAVMEEKTILACILREFWIESNQKREELGLAGDLILRPNNGIWIKLKRRHEDDP" }, "uniprot_id": "CP4V2_RAT", "enzyme_annotation": [ { "ec": "1.14.14.-", "activity": "" }, { "ec": "1.14.14.79", "activity": "Docosahexaenoate + [reduced NADPH--hemoprotein reductase] + O(2) = 22-hydroxydocosahexaenoate + [oxidized NADPH--hemoprotein reductase] + H(2)O." } ], "synonyms": { "protein": { "refseq": [ "cytochrome P450 4V2", "cytochrome P450 4V3; cytochrome P450-like protein; docosahexaenoic acid omega-hydroxylase CYP4V2" ], "uniprotkb": [ "Cytochrome P450 4V2", "1.14.14.-", "Docosahexaenoic acid omega-hydroxylase CYP4V2", "1.14.14.79" ] }, "gene": { "refseq": [ "Cyp4v3", "Cyp4v2" ], "uniprotkb": [ "Cyp4v2", "Cyp4v3" ] } }, "keywords": [], "species": [ { "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2RRT9", "id": "A2RRT9", "database": "UniProtKB" } ], "name": "Rattus norvegicus", "taxid": 10116 } ], "recommendedname": { "full": "Cytochrome P450 4V2; 1.14.14.-; Docosahexaenoic acid omega-hydroxylase CYP4V2; 1.14.14.79." }, "publication": [ { "title": "Expression and characterization of CYP4V2 as a fatty acid omega-hydroxylase.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/19661213", "journal": "Drug metabolism and disposition: the biological fate of chemicals", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001129072.1", "id": "NP_001129072.1", "database": "RefSeq" } ], "authors": "Nakano M, Kelly EJ, Rettie AE", "date": "2009", "pmid": "19661213" }, { "title": "Expression of tissue kallikrein mRNA in developing rat brains.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/12025956", "journal": "Canadian journal of physiology and pharmacology", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001129072.1", "id": "NP_001129072.1", "database": "RefSeq" } ], "authors": "Iwadate H, Kudo M, Kizuki K", "date": "2002", "pmid": "12025956" } ], "glycosylation": [], "mutation": [], "isoforms": [ { "url": "https://www.uniprot.org/uniprot/A2RRT9#A2RRT9-1", "isoform_ac": "A2RRT9-1", "locus": { "end_pos": 50112044, "start_pos": 50135365, "chromosome": "16", "evidence": [ { "url": "https://www.ensembl.org/id/ENSRNOT00000019302", "id": "ENSRNOT00000019302", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSRNOP00000019302", "id": "ENSRNOP00000019302", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "sp|A2RRT9-1|CP4V2_RAT Isoform 1 of Cytochrome P450 4V2 OS=Rattus norvegicus OX=10116 GN=Cyp4v2 PE=2 SV=1 CANONICAL=A2RRT9-1", "length": 525, "sequence": "MLWLWLGLSGQKLLLWGAASAVSVAGATVLLNILQMLVSYARKWQQMRPIPSVARAYPLVGHALFMKPNNTEFFQQIIQYTEEFRHLPIIKLWIGPVPLVALYKAENVEVILTSSKQIDKSFMYKFLQPWLGLGLLTSTGSKWRARRKMLTPSFHFTILEDFLDVMNEQANILVNKLEKHVNQEAFNCFFPITLCALDIICETAMGKNIGAQSNGDSEYVRTVYRMSDMIYRRMKMPWFWFDLWYLMFKEGRDHKKGLKSLHTFTNNVIAERVNARKAEQDCIGAGRGPLPSKTKRKAFLDLLLSVTDEEGNKLSHEDIREEVDTFMFEGHDTTAAAINWSLYLLGSNPEVQRKVDKELDDVFGRSHRPVTLEDLKKLKYLDCVIKETLRVFPSVPLFARSLSEDCEVAGYKISKGTEAVIIPYALHRDPRYFPDPEEFQPERFFPENSQGRHPYAYVPFSAGPRNCIGQKFAVMEEKTILACILREFWIESNQKREELGLAGDLILRPNNGIWIKLKRRHEDDP" } } ], "pathway": [ { "url": "https://www.genome.jp/dbget-bin/www_bget?rno:266761", "resource": "KEGG Pathway", "id": "rno:266761", "name": "" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-RNO-211976", "resource": "Reactome", "id": "R-RNO-211976", "name": "Endogenous sterols" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-RNO-2453902", "resource": "Reactome", "id": "R-RNO-2453902", "name": "The canonical retinoid cycle in rods (twilight vision)" } ], "function": [ { "url": "https://www.uniprot.org/uniprot/A2RRT9#function", "annotation": "Omega-hydroxylase that oxidizes medium-chain saturated fatty acids and polyunsaturated omega-3 fatty acids, and which plays a role in fatty acid and steroid metabolism in the eye. Catalyzes the omega-hydroxylation of medium-chain saturated fatty acids such as laurate, myristate and palmitate in an NADPH-dependent pathway. The substrate specificity is higher for myristate > laurate > palmitate (C14>C16>C12). Acts as a polyunsaturated omega-3 fatty acids hydroxylase by mediating oxidation of docosahexaenoate (DHA) to 22-hydroxydocosahexaenoate. Also produces some 21-hydroxydocosahexaenoate. Also converts eicosapentaenoate (EPA) to 20-hydroxyeicosapentaenoate (20-OH-EPA).", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2RRT9#function", "id": "A2RRT9", "database": "UniProtKB" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001129072.1", "annotation": "Tissue kallikrein mRNA expression is increased during prenatal and early postnatal development stages in rat brain.", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001129072.1", "id": "NP_001129072.1", "database": "RefSeq" } ] } ], "orthologs": [ { "uniprot_canonical_ac": "Q6ZWL3-1", "protein_name": "Cytochrome P450 4V2; 1.14.14.-; Docosahexaenoic acid omega-hydroxylase CYP4V2; 1.14.14.79.", "sequence": { "length": 525, "sequence": "MAGLWLGLVWQKLLLWGAASALSLAGASLVLSLLQRVASYARKWQQMRPIPTVARAYPLVGHALLMKPDGREFFQQIIEYTEEYRHMPLLKLWVGPVPMVALYNAENVEVILTSSKQIDKSSMYKFLEPWLGLGLLTSTGNKWRSRRKMLTPTFHFTILEDFLDIMNEQANILVKKLEKHINQEAFNCFFYITLCALDIICETAMGKNIGAQSNDDSEYVRAVYRMSEMIFRRIKMPWLWLDLWYLMFKEGWEHKKSLQILHTFTNSVIAERANEMNANEDCRGDGRGSAPSKNKRRAFLDLLLSVTDDEGNRLSHEDIREEVDTFMFEGHDTTAAAINWSLYLLGSNPEVQKKVDHELDDVFGKSDRPATVEDLKKLRYLECVIKETLRLFPSVPLFARSVSEDCEVAGYRVLKGTEAVIIPYALHRDPRYFPNPEEFQPERFFPENAQGRHPYAYVPFSAGPRNCIGQKFAVMEEKTILSCILRHFWIESNQKREELGLEGQLILRPSNGIWIKLKRRNADER" }, "evidence": [ { "url": "https://omabrowser.org/oma/group/818961/", "id": "818961", "database": "OMA" }, { "url": "http://www.informatics.jax.org/homology/133054", "id": "133054", "database": "MGI" } ], "organism": "Homo sapiens", "tax_id": 9606 }, { "uniprot_canonical_ac": "Q9DBW0-1", "protein_name": "Cytochrome P450 4V2; 1.14.14.-; Docosahexaenoic acid omega-hydroxylase CYP4V2; 1.14.14.79.", "sequence": { "length": 525, "sequence": "MLWLWLGLSGQKLLLWGAASAVSLAGATILISIFPMLVSYARKWQQMRSIPSVARAYPLVGHALYMKPNNAEFFQQLIYYTEEFRHLPIIKLWIGPVPLVALYKAENVEVILTSSKQIDKSFLYKFLQPWLGLGLLTSTGSKWRTRRKMLTPTFHFTILENFLDVMNEQANILVNKLEKHVNQEAFNCFFYITLCALDIICETAMGKNIGAQSNNDSEYVRTVYRMSDMIYRRMKMPWLWFDLWYLVFKEGRDHKRGLKCLHTFTNNVIAERVKERKAEEDWTGAGRGPIPSKNKRKAFLDLLLSVTDEEGNRLSQEDIREEVDTFMFEGHDTTAAAINWSLYLLGTNPEVQRKVDQELDEVFGRSHRPVTLEDLKKLKYLDCVIKETLRVFPSVPLFARSLSEDCEVGGYKVTKGTEAIIIPYALHRDPRYFPDPEEFRPERFFPENSQGRHPYAYVPFSAGPRNCIGQKFAVMEEKTILACILRQFWVESNQKREELGLAGDLILRPNNGIWIKLKRRHEDDP" }, "evidence": [ { "url": "https://omabrowser.org/oma/group/818961/", "id": "818961", "database": "OMA" }, { "url": "http://www.informatics.jax.org/homology/133054", "id": "133054", "database": "MGI" } ], "organism": "Mus musculus", "tax_id": 10090 } ], "refseq": { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001129072.1", "ac": "NP_001129072.1", "name": "cytochrome P450 4V2; cytochrome P450 4V3; cytochrome P450-like protein; docosahexaenoic acid omega-hydroxylase CYP4V2.", "summary": "" }, "go_annotation": { "categories": [ { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005506", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2RRT9", "id": "A2RRT9", "database": "UniProtKB" } ], "name": "iron ion binding", "id": "GO:0005506" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0020037", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2RRT9", "id": "A2RRT9", "database": "UniProtKB" } ], "name": "heme binding", "id": "GO:0020037" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0004497", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2RRT9", "id": "A2RRT9", "database": "UniProtKB" } ], "name": "monooxygenase activity", "id": "GO:0004497" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0016705", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2RRT9", "id": "A2RRT9", "database": "UniProtKB" } ], "name": "oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen", "id": "GO:0016705" } ], "total": 4, "name": "Molecular Function" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0016021", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2RRT9", "id": "A2RRT9", "database": "UniProtKB" } ], "name": "integral component of membrane", "id": "GO:0016021" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005789", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2RRT9", "id": "A2RRT9", "database": "UniProtKB" } ], "name": "endoplasmic reticulum membrane", "id": "GO:0005789" } ], "total": 2, "name": "Cellular Component" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0010430", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2RRT9", "id": "A2RRT9", "database": "UniProtKB" } ], "name": "fatty acid omega-oxidation", "id": "GO:0010430" } ], "total": 1, "name": "Biological Process" } ] }, "site_annotation": [ { "end_pos": 71, "annotation": "n_glycosylation_sequon", "start_pos": 69 }, { "end_pos": 341, "annotation": "n_glycosylation_sequon", "start_pos": 339 } ], "crossref": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001129072.1", "id": "NP_001129072.1", "database": "RefSeq" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-RNO-211976", "id": "R-RNO-211976", "database": "Reactome" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-RNO-2453902", "id": "R-RNO-2453902", "database": "Reactome" }, { "url": "https://www.genome.jp/dbget-bin/www_bget?rno:266761", "id": "rno:266761", "database": "KEGG Pathway" }, { "url": "https://bgee.org/?page=gene&gene_id=ENSRNOG00000042426", "id": "ENSRNOG00000042426", "database": "Bgee" }, { "url": "https://omabrowser.org/oma/group/IQPAFHK/", "id": "IQPAFHK", "database": "OMA" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR001128", "id": "IPR001128", "database": "InterPro" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR002401", "id": "IPR002401", "database": "InterPro" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR017972", "id": "IPR017972", "database": "InterPro" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR036396", "id": "IPR036396", "database": "InterPro" }, { "url": "http://pfam.xfam.org/family/PF00067", "id": "PF00067", "database": "Pfam" }, { "url": "http://research.bioinformatics.udel.edu/pro/entry/PR_A2RRT9", "id": "PR_A2RRT9", "database": "PRO" }, { "url": "https://www.ncbi.nlm.nih.gov/gene/266761", "id": "266761", "database": "GeneID" }, { "url": "http://rgd.mcw.edu/tools/genes/genes_view.cgi?id=708530", "id": "708530", "database": "RGD" } ], "expression_disease": [], "uniprot_ac": "A2RRT9", "disease": [], "mass": { "monoisotopic_mass": 60580.0, "chemical_mass": 60580.0 }, "expression_tissue": [], "gene": [ { "url": "https://www.uniprot.org/uniprot/A2RRT9", "name": "Cyp4v2; Cyp4v3.", "locus": { "end_pos": 0, "start_pos": 0, "chromosome": "N/A", "evidence": [] } } ] }