{ "uniprot_canonical_ac": "A2APC3-1", "sequence": { "header": "sp|A2APC3-1|TTLL9_MOUSE Isoform 1 of Probable tubulin polyglutamylase TTLL9 OS=Mus musculus OX=10090 GN=Ttll9 PE=2 SV=2 CANONICAL=A2APC3-1", "length": 461, "sequence": "MSRQKNQNSKGHGVSKGKEREQRTLIRFKTTLMNTLMDVLRHRPGWVEVKDEGEWDFYWCDVSWLRENFDHTYMDEHVRISHFRNHYELTRKNYMVKNLKRFRKYLERESGKTEAAKCDFFPKTFEMPCEYHLFVEEFRKNPGITWIMKPVARSQGKGIFLFRRLKDIMDWRKGTSGKKPTGVETQPARANMNPSGSHDTRSSDDQKDDLPVENYVAQRYVENPYLIGGRKFDLRVYVLVMSYIPLRAWLYRDGFARFSNTRFTLNSIDDHYVHLTNVAVQKTSPDYHLKKGCKWMLQRFRQYLASKHGPKAVETLFSDMDNIFIKSLQSVQKVIISDKHCFELYGYDILIDQDLKPWLLEVNASPSLTASSQEDYELKTCLLEDTLHVVDMEARLTGKEKRVGGFDLMWNDGPVSREDGPSDLSGMGNFVTNTHLGCVNDRKEQLRQLFRSLQAQRKAPS" }, "uniprot_id": "TTLL9_MOUSE", "enzyme_annotation": [ { "ec": "6.-.-.-", "activity": "" } ], "synonyms": { "protein": { "refseq": [ "probable tubulin polyglutamylase TTLL9 isoform 1", "tubulin tyrosine ligase like family 9; probable tubulin polyglutamylase TTLL9; putative polyglutamylase; tubulin--tyrosine ligase-like protein 9" ], "uniprotkb": [ "Probable tubulin polyglutamylase TTLL9", "6.-.-.-", "Tubulin--tyrosine ligase-like protein 9" ] }, "gene": { "refseq": [ "Ttll9", "1700016F23Rik", "4930509O20Rik", "AW046572" ], "uniprotkb": [ "Ttll9", "1700016F23Rik", "4930509O20Rik", "AW046572" ] } }, "keywords": [], "species": [ { "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "id": "A2APC3", "database": "UniProtKB" } ], "name": "Mus musculus", "taxid": 10090 } ], "recommendedname": { "full": "Probable tubulin polyglutamylase TTLL9; 6.-.-.-; Tubulin--tyrosine ligase-like protein 9." }, "publication": [ { "title": "Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/29593216", "journal": "Nature communications", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077087.1", "id": "NP_001077087.1", "database": "RefSeq" } ], "authors": "Ye B, Liu B, Hao L, Zhu X, Yang L, Wang S, Xia P, Du Y, Meng S, Huang G, Qin X, Wang Y, Yan X, Li C, Hao J, Zhu P, He L, Tian Y, Fan Z", "date": "2018", "pmid": "29593216" }, { "title": "Ttll9-/- mice sperm flagella show shortening of doublet 7, reduction of doublet 5 polyglutamylation and a stall in beating.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/27257088", "journal": "J. Cell Sci.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "id": "A2APC3", "database": "UniProtKB" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077087.1", "id": "NP_001077087.1", "database": "RefSeq" } ], "authors": "Abe M., Yamazaki M., Shiba K., Setou M., Yao I., Ikegami K., Yang H.J., Konishi Y., Inaba K., Sakimura K., Konno A.", "date": "2016", "pmid": "27257088" }, { "title": "A targeted multienzyme mechanism for selective microtubule polyglutamylation.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/17499049", "journal": "Mol. Cell", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "id": "A2APC3", "database": "UniProtKB" }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077087.1", "id": "NP_001077087.1", "database": "RefSeq" } ], "authors": "Janke C., Lacroix B., Rogowski K., van Dijk J., Miro J., Edde B.", "date": "2007", "pmid": "17499049" } ], "glycosylation": [], "mutation": [], "isoforms": [ { "url": "https://www.uniprot.org/uniprot/A2APC3#A2APC3-1", "isoform_ac": "A2APC3-1", "locus": { "end_pos": 152962541, "start_pos": 153006982, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000099197", "id": "ENSMUST00000099197", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000096803", "id": "ENSMUSP00000096803", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "sp|A2APC3-1|TTLL9_MOUSE Isoform 1 of Probable tubulin polyglutamylase TTLL9 OS=Mus musculus OX=10090 GN=Ttll9 PE=2 SV=2 CANONICAL=A2APC3-1", "length": 461, "sequence": "MSRQKNQNSKGHGVSKGKEREQRTLIRFKTTLMNTLMDVLRHRPGWVEVKDEGEWDFYWCDVSWLRENFDHTYMDEHVRISHFRNHYELTRKNYMVKNLKRFRKYLERESGKTEAAKCDFFPKTFEMPCEYHLFVEEFRKNPGITWIMKPVARSQGKGIFLFRRLKDIMDWRKGTSGKKPTGVETQPARANMNPSGSHDTRSSDDQKDDLPVENYVAQRYVENPYLIGGRKFDLRVYVLVMSYIPLRAWLYRDGFARFSNTRFTLNSIDDHYVHLTNVAVQKTSPDYHLKKGCKWMLQRFRQYLASKHGPKAVETLFSDMDNIFIKSLQSVQKVIISDKHCFELYGYDILIDQDLKPWLLEVNASPSLTASSQEDYELKTCLLEDTLHVVDMEARLTGKEKRVGGFDLMWNDGPVSREDGPSDLSGMGNFVTNTHLGCVNDRKEQLRQLFRSLQAQRKAPS" } }, { "url": "https://www.uniprot.org/uniprot/A2APC3#Z4YLM0-1", "isoform_ac": "Z4YLM0-1", "locus": { "end_pos": 152973579, "start_pos": 152984321, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000155631", "id": "ENSMUST00000155631", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000114786", "id": "ENSMUSP00000114786", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|Z4YLM0-1|TTLL9_MOUSE Computationally mapped isoform of Probable tubulin polyglutamylase TTLL9 OS=Mus musculus OX=10090 GN=Ttll9 PE=2 SV=1 CANONICAL=A2APC3-1", "length": 139, "sequence": "MTCDPRGSKEQRTLIRFKTTLMNTLMDVLRHRPGWVEVKDEGEWDFYWCDVSWLRENFDHTYMDEHVRISHFRNHYELTRKNYMVKNLKRFRKYLERESGKTEAAKCDFFPKTFEMPCEYHLFVEEFRKNPGITWIMKP" } }, { "url": "https://www.uniprot.org/uniprot/A2APC3#A2APC3-2", "isoform_ac": "A2APC3-2", "locus": { "end_pos": 152962541, "start_pos": 153003105, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000103155", "id": "ENSMUST00000103155", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000099444", "id": "ENSMUSP00000099444", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "sp|A2APC3-2|TTLL9_MOUSE Isoform 2 of Probable tubulin polyglutamylase TTLL9 OS=Mus musculus OX=10090 GN=Ttll9 PE=2 SV=2 CANONICAL=A2APC3-1", "length": 464, "sequence": "MSRQKNQNSKGHGVSKGKEREQRTLIRFKTTLMNTLMDVLRHRPGWVEVKDEGEWDFYWCDVSWLRENFDHTYMDEHVRISHFRNHYELTRKNYMVKNLKRFRKYLERESGKTEAAKCDFFPKTFEMPCEYHLFVEEFRKNPGITWIMKPVARSQGKGIFLFRRLKDIMDWRKGTSGKKPTGVETQPARANMNPSGSHDTRSSDDQKDDLPVENYVAQRYVENPYLIGGRKFDLRVYVLVMSYIPLRAWLYRDGFARFSNTRFTLNSIDDHYVHLTNVAVQKTSPDYHLKKGCKWMLQRFRQYLASKHGPKAVETLFSDMDNIFIKSLQSVQKVIISDKHCFELYGYDILIDQDLKPWLLEVNASPSLTASSQEDYELKTCLLEDTLHVVDMEARLTGKEKRVGGFDLMWNDGPVSREDGPSDLSGMGNFVTNTHLGTLSQTGSEWERNIIGSGGGLGPLGRAV" } }, { "url": "https://www.uniprot.org/uniprot/A2APC3#E9PUG4-1", "isoform_ac": "E9PUG4-1", "locus": { "end_pos": 152962541, "start_pos": 152999349, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000109801", "id": "ENSMUST00000109801", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000105426", "id": "ENSMUSP00000105426", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|E9PUG4-1|TTLL9_MOUSE Computationally mapped isoform of Probable tubulin polyglutamylase TTLL9 OS=Mus musculus OX=10090 GN=Ttll9 PE=2 SV=1 CANONICAL=A2APC3-1", "length": 234, "sequence": "MSRQKNQNSKGHGVSKGKEREQRTLIRFKTTLMNTLMDVLRHRPGWVEVKDEGEWDFYWCDVSWLRENFDHTYMDEHVRISHFRNHYELTRKNYMVKNLKRFRKYLERESGKTEAAKCDFFPKTFEMPCEYHLFVEEFRKNPGITWIMKPVARSQGKGIFLFRRLKDIMDWRKDTRSSDDQKDDLPVENYVAQRYVENPYLIGGRKFDLRVYVLVMSMFTSPMLLCRRHRLTTT" } }, { "url": "https://www.uniprot.org/uniprot/A2APC3#D6RCJ6-1", "isoform_ac": "D6RCJ6-1", "locus": { "end_pos": 152962541, "start_pos": 152976243, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000146626", "id": "ENSMUST00000146626", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000121434", "id": "ENSMUSP00000121434", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|D6RCJ6-1|TTLL9_MOUSE Computationally mapped isoform of Probable tubulin polyglutamylase TTLL9 OS=Mus musculus OX=10090 GN=Ttll9 PE=2 SV=1 CANONICAL=A2APC3-1", "length": 36, "sequence": "MSRQKRAENLNPLQDHPHEHTHGCSAPQARMGGSQR" } }, { "url": "https://www.uniprot.org/uniprot/A2APC3#D6RG93-1", "isoform_ac": "D6RG93-1", "locus": { "end_pos": 152962541, "start_pos": 152984194, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000152158", "id": "ENSMUST00000152158", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000122494", "id": "ENSMUSP00000122494", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|D6RG93-1|TTLL9_MOUSE Computationally mapped isoform of Probable tubulin polyglutamylase TTLL9 OS=Mus musculus OX=10090 GN=Ttll9 PE=2 SV=1 CANONICAL=A2APC3-1", "length": 126, "sequence": "MSRQKNQNSKGHGVSKGKEREQRTLIRFKTTLMNTLMDVLRHRPGWVEVKDEGEWDFYWCDVSWLRENFDHTYMDEHVRISHFRNHYEKWARQGTEGSPSALPCCLAYQEKLHGKEPEALPEVPGA" } }, { "url": "https://www.uniprot.org/uniprot/A2APC3#F6ZRM1-1", "isoform_ac": "F6ZRM1-1", "locus": { "end_pos": 152972288, "start_pos": 152991659, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000165343", "id": "ENSMUST00000165343", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000131839", "id": "ENSMUSP00000131839", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|F6ZRM1-1|TTLL9_MOUSE Computationally mapped isoform of Probable tubulin polyglutamylase TTLL9 OS=Mus musculus OX=10090 GN=Ttll9 PE=2 SV=1 CANONICAL=A2APC3-1", "length": 73, "sequence": "NQNSKGHGVSKGKEREQRTLIRFKTTLMNTLMDVLRHRPGWVEVKETQGVLMTRKMTSRWRTTWLSVTWKTPT" } } ], "pathway": [ { "url": "https://www.genome.jp/dbget-bin/www_bget?mmu:74711", "resource": "KEGG Pathway", "id": "mmu:74711", "name": "" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-8955332", "resource": "Reactome", "id": "R-MMU-8955332", "name": "Carboxyterminal post-translational modifications of tubulin" } ], "function": [ { "url": "https://www.uniprot.org/uniprot/A2APC3#function", "annotation": "Probable tubulin polyglutamylase that forms polyglutamate side chains on tubulin (PubMed:27257088). Acts when complexed with other proteins (Probable). By mediating tubulin polyglutamylation, plays a role in the establishment of microtubule heterogeneity in sperm flagella (PubMed:27257088).", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3#function", "id": "A2APC3", "database": "UniProtKB" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077087.1", "annotation": "Ttll9 plays essential roles in maintaining the normal structure and beating pattern of sperm flagella by establishing normal heterogeneous polyglutamylation patterns.", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077087.1", "id": "NP_001077087.1", "database": "RefSeq" } ] } ], "orthologs": [ { "uniprot_canonical_ac": "Q641W7-1", "protein_name": "Probable tubulin polyglutamylase TTLL9; 6.-.-.-; Tubulin--tyrosine ligase-like protein 9.", "sequence": { "length": 461, "sequence": "MSRQKSQTSKGHGASKGKEREQRTLIRFKTTLMNTLMDVLRHRPGWVEVKDEGEWDFYWCDVSWLRENFDHTYMDEHVRISHFRNHYELTRKNYMVKNLKRFRKQLEREAGKTEAAKCDFFPKTFEMPCEYHLFVEEFRKNPGITWIMKPVARSQGKGIFLFRRLKDIMDWRKGTAGKKVTSVETQATRANVNPSGSHDTRSSDDQKDDIPVENYVAQRYVENPYLIGGRKFDLRVYVLVMSYIPLRAWLYRDGFARFSNTRFTLNSIDDHYVHLTNVAVQKTSPDYHPKKGCKWTLQRFRQYLASKHGPKAVETLFSDMDNIFIKSLQSVQKVIISDKHCFELYGYDILIDQDLKPWLLEVNASPSLTASSQEDYELKTCLLEDTLHVVDMEARLTGKEKRVGGFDLMWNDGPVSREEGPCDLSGMGNFVTNTHLGCINDRKEQLRQLFRSLQVQKKASS" }, "evidence": [ { "url": "http://www.informatics.jax.org/homology/41769", "id": "41769", "database": "MGI" }, { "url": "https://omabrowser.org/oma/group/494605/", "id": "494605", "database": "OMA" } ], "organism": "Rattus norvegicus", "tax_id": 10116 }, { "uniprot_canonical_ac": "Q3SXZ7-1", "protein_name": "Probable tubulin polyglutamylase TTLL9; 6.-.-.-; Tubulin--tyrosine ligase-like protein 9.", "sequence": { "length": 439, "sequence": "MVPSREALLGPGTTAIRCPKKLQNQNYKGHGLSKGKEREQRASIRFKTTLMNTLMDVLRHRPGWVEVKDEGEWDFYWCDVSWLRENFDHTYMDEHVRISHFRNHYELTRKNYMVKNLKRFRKQLEREAGKLEAAKCDFFPKTFEMPCEYHLFVEEFRKNPGITWIMKPVARSQGKGIFLFRRLKDIVDWRKDTRSSDDQKDDIPVENYVAQRYIENPYLIGGRKFDLRVYVLVMSVFAECLLWSGHRRQDVHLTNVAVQKTSPDYHPKKGCKWTLQRFRQYLASKHGPEAVETLFRDIDNIFVKSLQSVQKVIISDKHCFELYGYDILIDQDLKPWLLEVNASPSLTASSQEDYELKTCLLEDTLHVVDMEARLTGREKRVGGFDLMWNDGPVSREEGAPDLSGMGNFVTNTHLGCVNDRKKQLRQLFCSLQVQKKASS" }, "evidence": [ { "url": "http://www.informatics.jax.org/homology/41769", "id": "41769", "database": "MGI" }, { "url": "https://omabrowser.org/oma/group/494605/", "id": "494605", "database": "OMA" } ], "organism": "Homo sapiens", "tax_id": 9606 } ], "refseq": { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077087.1", "ac": "NP_001077087.1", "name": "probable tubulin polyglutamylase TTLL9 isoform 1; tubulin tyrosine ligase like family 9; probable tubulin polyglutamylase TTLL9; putative polyglutamylase; tubulin--tyrosine ligase-like protein 9.", "summary": "" }, "go_annotation": { "categories": [ { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005524", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "id": "A2APC3", "database": "UniProtKB" } ], "name": "ATP binding", "id": "GO:0005524" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0016874", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "id": "A2APC3", "database": "UniProtKB" } ], "name": "ligase activity", "id": "GO:0016874" } ], "total": 2, "name": "Molecular Function" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005737", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "id": "A2APC3", "database": "UniProtKB" } ], "name": "cytoplasm", "id": "GO:0005737" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005874", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "id": "A2APC3", "database": "UniProtKB" } ], "name": "microtubule", "id": "GO:0005874" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005929", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "id": "A2APC3", "database": "UniProtKB" } ], "name": "cilium", "id": "GO:0005929" } ], "total": 3, "name": "Cellular Component" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0006464", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "id": "A2APC3", "database": "UniProtKB" } ], "name": "cellular protein modification process", "id": "GO:0006464" } ], "total": 1, "name": "Biological Process" } ] }, "site_annotation": [ { "end_pos": 365, "annotation": "n_glycosylation_sequon", "start_pos": 363 } ], "crossref": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001077087.1", "id": "NP_001077087.1", "database": "RefSeq" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-8955332", "id": "R-MMU-8955332", "database": "Reactome" }, { "url": "https://www.genome.jp/dbget-bin/www_bget?mmu:74711", "id": "mmu:74711", "database": "KEGG Pathway" }, { "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000074673", "id": "ENSMUSG00000074673", "database": "Bgee" }, { "url": "https://omabrowser.org/oma/group/YVPLKAW/", "id": "YVPLKAW", "database": "OMA" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR004344", "id": "IPR004344", "database": "InterPro" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR027751", "id": "IPR027751", "database": "InterPro" }, { "url": "http://pfam.xfam.org/family/PF03133", "id": "PF03133", "database": "Pfam" }, { "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR12241:SF39", "id": "PTHR12241:SF39", "database": "PANTHER" }, { "url": "http://research.bioinformatics.udel.edu/pro/entry/PR_A2APC3", "id": "PR_A2APC3", "database": "PRO" }, { "url": "https://www.ncbi.nlm.nih.gov/gene/74711", "id": "74711", "database": "GeneID" }, { "url": "http://www.informatics.jax.org/marker/MGI:1913589", "id": "1913589", "database": "MGI" } ], "expression_disease": [], "uniprot_ac": "A2APC3", "disease": [], "mass": { "monoisotopic_mass": 54107.0, "chemical_mass": 54107.0 }, "expression_tissue": [], "gene": [ { "url": "https://www.uniprot.org/uniprot/A2APC3", "name": "Ttll9; 1700016F23Rik; 4930509O20Rik; AW046572.", "locus": { "end_pos": 153008482, "start_pos": 152962485, "chromosome": "2", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUSG00000074673", "id": "ENSMUSG00000074673", "database": "Ensembl Gene" } ] } } ] }