{
    "uniprot_canonical_ac": "A0A4Z3-1", 
    "sequence": {
        "header": "sp|A0A4Z3-1|A3LT2_RAT Isoform 1 of Alpha-1,3-galactosyltransferase 2  OS=Rattus norvegicus OX=10116 GN=A3galt2 PE=1 SV=1 CANONICAL=A0A4Z3-1", 
        "length": 339, 
        "sequence": "MALEGLRAKKRLLWRLFLSAFGLLGLYHYWFKIFRLFEVFIPMGICPMAIMPLLKDNFTGVLRHWARPEVLTCTSWGAPIIWDETFDPHVAEREARRQNLTIGLTVFAVGRYLEKYLEHFLVSAEQYFMVGQNVVYYVFTDRPEAVPHVALGQGRLLRVKPVRREKRWQDVSMARMLTLHEALGGQLGREADYVFCLDVDQYFSGNFGPEVLADLVAQLHAWHFRWPRWMLPYERDKRSAAALSLSEGDFYYHAAVFGGSVAALLKLTAHCATGQQLDREHGIEARWHDESHLNKFFWLSKPTKLLSPEFCWAEEIGWRPEIHHPRLIWAPKEYALVRT"
    }, 
    "uniprot_id": "A3LT2_RAT", 
    "enzyme_annotation": [
        {
            "ec": "2.4.1.87", 
            "activity": "UDP-alpha-D-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R."
        }, 
        {
            "ec": "2.4.1.87", 
            "activity": ""
        }
    ], 
    "synonyms": {
        "protein": {
            "refseq": [
                "alpha-1,3-galactosyltransferase 2", 
                "iGb3S; isoglobotriaosylceramide synthase; iGb3 synthase"
            ], 
            "uniprotkb": [
                "Alpha-1,3-galactosyltransferase 2", 
                "2.4.1.87", 
                "Isoglobotriaosylceramide synthase", 
                "iGb3 synthase", 
                "iGb3S"
            ]
        }, 
        "gene": {
            "refseq": [
                "A3galt2"
            ], 
            "uniprotkb": [
                "A3galt2"
            ]
        }
    }, 
    "keywords": [
        "glycosyltransferase-activity"
    ], 
    "species": [
        {
            "evidence": [
                {
                    "url": "https://www.uniprot.org/uniprot/A0A4Z3", 
                    "id": "A0A4Z3", 
                    "database": "UniProtKB"
                }
            ], 
            "name": "Rattus norvegicus", 
            "taxid": 10116
        }
    ], 
    "recommendedname": {
        "full": "Alpha-1,3-galactosyltransferase 2; 2.4.1.87; Isoglobotriaosylceramide synthase; iGb3 synthase; iGb3S."
    }, 
    "publication": [
        {
            "title": "Globosides but not isoglobosides can impact the development of invariant NKT cells and their interaction with dendritic cells.", 
            "url": "https://www.ncbi.nlm.nih.gov/pubmed/22875802", 
            "journal": "Journal of immunology (Baltimore, Md. : 1950)", 
            "evidence": [
                {
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_612533.1", 
                    "id": "NP_612533.1", 
                    "database": "RefSeq"
                }
            ], 
            "authors": "Porubsky S, Speak AO, Salio M, Jennemann R, Bonrouhi M, Zafarulla R, Singh Y, Dyson J, Luckow B, Lehuen A, Malle E, Muthing J, Platt FM, Cerundolo V, Grone HJ", 
            "date": "2012", 
            "pmid": "22875802"
        }, 
        {
            "title": "Humans lack iGb3 due to the absence of functional iGb3-synthase: implications for NKT cell development and transplantation.", 
            "url": "https://www.ncbi.nlm.nih.gov/pubmed/18630988", 
            "journal": "PLoS Biol.", 
            "evidence": [
                {
                    "url": "https://www.uniprot.org/uniprot/A0A4Z3", 
                    "id": "A0A4Z3", 
                    "database": "UniProtKB"
                }, 
                {
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_612533.1", 
                    "id": "NP_612533.1", 
                    "database": "RefSeq"
                }
            ], 
            "authors": "McConville M.J., Christiansen D., Godfrey D.I., Milland J., Mouhtouris E., Pellicci D.G., Sandrin M.S., Vaughan H.", 
            "date": "2008", 
            "pmid": "18630988"
        }, 
        {
            "title": "Enzymes that synthesize the IB4 epitope are not sufficient to impart IB4 binding in dorsal root ganglia of rat.", 
            "url": "https://www.ncbi.nlm.nih.gov/pubmed/17206613", 
            "journal": "J. Comp. Neurol.", 
            "evidence": [
                {
                    "url": "https://www.uniprot.org/uniprot/A0A4Z3", 
                    "id": "A0A4Z3", 
                    "database": "UniProtKB"
                }, 
                {
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_612533.1", 
                    "id": "NP_612533.1", 
                    "database": "RefSeq"
                }
            ], 
            "authors": "Elde R., Fullmer J.M., Riedl M., Sandrin M., Williams F.G.", 
            "date": "2007", 
            "pmid": "17206613"
        }, 
        {
            "title": "Characterization of the rat alpha(1,3)galactosyltransferase: evidence for two independent genes encoding glycosyltransferases that synthesize Galalpha(1,3)Gal by two separate glycosylation pathways.", 
            "url": "https://www.ncbi.nlm.nih.gov/pubmed/12626403", 
            "journal": "Glycobiology", 
            "evidence": [
                {
                    "url": "https://www.uniprot.org/uniprot/A0A4Z3", 
                    "id": "A0A4Z3", 
                    "database": "UniProtKB"
                }, 
                {
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_612533.1", 
                    "id": "NP_612533.1", 
                    "database": "RefSeq"
                }
            ], 
            "authors": "Sandrin M.S., McKenzie I.F., Taylor S.G.", 
            "date": "2003", 
            "pmid": "12626403"
        }, 
        {
            "title": "Expression cloning of a new member of the ABO blood group glycosyltransferases, iGb3 synthase, that directs the synthesis of isoglobo-glycosphingolipids.", 
            "url": "https://www.ncbi.nlm.nih.gov/pubmed/10854427", 
            "journal": "J. Biol. Chem.", 
            "evidence": [
                {
                    "url": "https://www.uniprot.org/uniprot/A0A4Z3", 
                    "id": "A0A4Z3", 
                    "database": "UniProtKB"
                }, 
                {
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_612533.1", 
                    "id": "NP_612533.1", 
                    "database": "RefSeq"
                }
            ], 
            "authors": "Keusch J.J., Cummings R.D., Baenziger J.U., Manzella S.M., Nyame K.A.", 
            "date": "2000", 
            "pmid": "10854427"
        }, 
        {
            "title": "UDP-galactose:globotriaosylceramide alpha-galactosyltransferase activity in rat pheochromocytoma (PC12h) cells.", 
            "url": "https://www.ncbi.nlm.nih.gov/pubmed/1569388", 
            "journal": "Journal of lipid research", 
            "evidence": [
                {
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_612533.1", 
                    "id": "NP_612533.1", 
                    "database": "RefSeq"
                }
            ], 
            "authors": "Pal S, Saito M, Ariga T, Yu RK", 
            "date": "1992", 
            "pmid": "1569388"
        }
    ], 
    "glycosylation": [
        {
            "residue": "Asn", 
            "evidence": [
                {
                    "url": "https://www.uniprot.org/uniprot/A0A4Z3#PTM_processing", 
                    "id": "A0A4Z3", 
                    "database": "UniProtKB"
                }
            ], 
            "relation": "attached", 
            "position": 99, 
            "glytoucan_ac": "", 
            "type": "N-linked"
        }, 
        {
            "residue": "Asn", 
            "evidence": [
                {
                    "url": "https://www.uniprot.org/uniprot/A0A4Z3#PTM_processing", 
                    "id": "A0A4Z3", 
                    "database": "UniProtKB"
                }
            ], 
            "relation": "attached", 
            "position": 57, 
            "glytoucan_ac": "", 
            "type": "N-linked"
        }
    ], 
    "mutation": [], 
    "isoforms": [
        {
            "url": "https://www.uniprot.org/uniprot/A0A4Z3#A0A4Z3-1", 
            "isoform_ac": "A0A4Z3-1", 
            "locus": {
                "end_pos": 146933669, 
                "start_pos": 146945065, 
                "chromosome": "5", 
                "evidence": [
                    {
                        "url": "https://www.ensembl.org/id/ENSRNOT00000007851", 
                        "id": "ENSRNOT00000007851", 
                        "database": "Ensembl Transcript"
                    }, 
                    {
                        "url": "https://www.ensembl.org/id/ENSRNOP00000007851", 
                        "id": "ENSRNOP00000007851", 
                        "database": "Ensembl Peptide"
                    }
                ]
            }, 
            "sequence": {
                "header": "sp|A0A4Z3-1|A3LT2_RAT Isoform 1 of Alpha-1,3-galactosyltransferase 2  OS=Rattus norvegicus OX=10116 GN=A3galt2 PE=1 SV=1 CANONICAL=A0A4Z3-1", 
                "length": 339, 
                "sequence": "MALEGLRAKKRLLWRLFLSAFGLLGLYHYWFKIFRLFEVFIPMGICPMAIMPLLKDNFTGVLRHWARPEVLTCTSWGAPIIWDETFDPHVAEREARRQNLTIGLTVFAVGRYLEKYLEHFLVSAEQYFMVGQNVVYYVFTDRPEAVPHVALGQGRLLRVKPVRREKRWQDVSMARMLTLHEALGGQLGREADYVFCLDVDQYFSGNFGPEVLADLVAQLHAWHFRWPRWMLPYERDKRSAAALSLSEGDFYYHAAVFGGSVAALLKLTAHCATGQQLDREHGIEARWHDESHLNKFFWLSKPTKLLSPEFCWAEEIGWRPEIHHPRLIWAPKEYALVRT"
            }
        }
    ], 
    "pathway": [
        {
            "url": "https://www.genome.jp/dbget-bin/www_bget?rno:171553", 
            "resource": "KEGG Pathway", 
            "id": "rno:171553", 
            "name": ""
        }
    ], 
    "function": [
        {
            "url": "https://www.uniprot.org/uniprot/A0A4Z3#function", 
            "annotation": "Synthesizes the galactose-alpha(1,3)-galactose group on the glycosphingolipid isoglobotrihexosylceramide or isogloboside 3 (iGb3) by catalyzing the transfer of galactose from UDP-Galactose to its acceptor molecule Gal-beta-1,4-Glc-ceramide. Can also catalyze the addition of galactose to iGb3 itself to form polygalactose structures. Synthesis of iGb3 is the initial step in the formation of the isoglobo-series glycolipid pathway and is the precursor to isogloboside 4 (iGb4) and isoForssman glycolipids. Can glycosylate only lipids and not proteins and is solely responsible for initiating the synthesis of isoglobo-series glycosphingolipids.", 
            "evidence": [
                {
                    "url": "https://www.uniprot.org/uniprot/A0A4Z3#function", 
                    "id": "A0A4Z3", 
                    "database": "UniProtKB"
                }
            ]
        }
    ], 
    "orthologs": [
        {
            "uniprot_canonical_ac": "U3KPV4-1", 
            "protein_name": "Alpha-1,3-galactosyltransferase 2; 2.4.1.87; Isoglobotriaosylceramide synthase; iGb3 synthase; iGb3S.", 
            "sequence": {
                "length": 340, 
                "sequence": "MALKEGLRAWKRIFWRQILLTLGLLGLFLYGLPKFRHLEALIPMGVCPSATMSQLRDNFTGALRPWARPEVLTCTPWGAPIIWDGSFDPDVAKQEARQQNLTIGLTIFAVGRYLEKYLERFLETAEQHFMAGQSVMYYVFTELPGAVPRVALGPGRRLPVERVARERRWQDVSMARMRTLHAALGGLPGREAHFMFCMDVDQHFSGTFGPEALAESVAQLHSWHYHWPSWLLPFERDAHSAAAMAWGQGDFYNHAAVFGGSVAALRGLTAHCAGGLDWDRARGLEARWHDESHLNKFFWLHKPAKVLSPEFCWSPDIGPRAEIRRPRLLWAPKGYRLLRN"
            }, 
            "evidence": [
                {
                    "url": "https://omabrowser.org/oma/group/670351/", 
                    "id": "670351", 
                    "database": "OMA"
                }, 
                {
                    "url": "http://www.informatics.jax.org/homology/16326", 
                    "id": "16326", 
                    "database": "MGI"
                }
            ], 
            "organism": "Homo sapiens", 
            "tax_id": 9606
        }, 
        {
            "uniprot_canonical_ac": "Q3V1N9-1", 
            "protein_name": "Alpha-1,3-galactosyltransferase 2; 2.4.1.87; Isoglobotriaosylceramide synthase; iGb3 synthase; iGb3S.", 
            "sequence": {
                "length": 370, 
                "sequence": "MALGTELGVSWPGSHGSCREQEGQRQRGPGKPTWGLSRAKKRLLWRFFLSAFGFLGLYHYRFIIIRLIEGSIPMGTCPTAIMPLPRDNFTGVLHHWARPEVLTCTSWGAPIIWDGTFDPHVAQQEARRRNLTIGLTVFAVGRYLEKYLEHFLVSAEQHFMVGQNVVYYVFTDRPEAVPYVALGQGRLLRAKPVQRERRWQDVSMARMPTLHEALGGQLGQEADFVFCLDVDQYFTGNFGPEVLADLVAQLHAWHYRWPRWLLPYERDKRSAAALSLSEGDFYYHAAVFGGSVAALLKLTAHCATGQQLDHKRGIEALWHDESHLNKFFWLNKPTKLLSPEFCWAEEIIWRREIHHPRLLWAPKEYTLVRN"
            }, 
            "evidence": [
                {
                    "url": "http://www.informatics.jax.org/homology/16326", 
                    "id": "16326", 
                    "database": "MGI"
                }
            ], 
            "organism": "Mus musculus", 
            "tax_id": 10090
        }
    ], 
    "refseq": {
        "url": "https://www.ncbi.nlm.nih.gov/protein/NP_612533.1", 
        "ac": "NP_612533.1", 
        "name": "alpha-1,3-galactosyltransferase 2; iGb3S; isoglobotriaosylceramide synthase; iGb3 synthase.", 
        "summary": "UDP-galactose: beta-d-galactosyl-1,4-glucosylceramide alpha-1, 3-galactosyltransferase; involved in the synthesis of the isoglobo-series of glycosphingolipids [RGD, Feb 2006]."
    }, 
    "go_annotation": {
        "categories": [
            {
                "go_terms": [
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0016757", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A0A4Z3", 
                                "id": "A0A4Z3", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "transferase activity, transferring glycosyl groups", 
                        "id": "GO:0016757"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0047276", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A0A4Z3", 
                                "id": "A0A4Z3", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "N-acetyllactosaminide 3-alpha-galactosyltransferase activity", 
                        "id": "GO:0047276"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0046872", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A0A4Z3", 
                                "id": "A0A4Z3", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "metal ion binding", 
                        "id": "GO:0046872"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0001962", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A0A4Z3", 
                                "id": "A0A4Z3", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "alpha-1,3-galactosyltransferase activity", 
                        "id": "GO:0001962"
                    }
                ], 
                "total": 4, 
                "name": "Molecular Function"
            }, 
            {
                "go_terms": [
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005794", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A0A4Z3", 
                                "id": "A0A4Z3", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "Golgi apparatus", 
                        "id": "GO:0005794"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0031982", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A0A4Z3", 
                                "id": "A0A4Z3", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "vesicle", 
                        "id": "GO:0031982"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0016021", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A0A4Z3", 
                                "id": "A0A4Z3", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "integral component of membrane", 
                        "id": "GO:0016021"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0032580", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A0A4Z3", 
                                "id": "A0A4Z3", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "Golgi cisterna membrane", 
                        "id": "GO:0032580"
                    }
                ], 
                "total": 4, 
                "name": "Cellular Component"
            }, 
            {
                "go_terms": [
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0006688", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A0A4Z3", 
                                "id": "A0A4Z3", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "glycosphingolipid biosynthetic process", 
                        "id": "GO:0006688"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0030259", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A0A4Z3", 
                                "id": "A0A4Z3", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "lipid glycosylation", 
                        "id": "GO:0030259"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0071287", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A0A4Z3", 
                                "id": "A0A4Z3", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "cellular response to manganese ion", 
                        "id": "GO:0071287"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005975", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A0A4Z3", 
                                "id": "A0A4Z3", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "carbohydrate metabolic process", 
                        "id": "GO:0005975"
                    }
                ], 
                "total": 4, 
                "name": "Biological Process"
            }
        ]
    }, 
    "site_annotation": [
        {
            "end_pos": 59, 
            "annotation": "n_glycosylation_sequon", 
            "start_pos": 57
        }, 
        {
            "end_pos": 101, 
            "annotation": "n_glycosylation_sequon", 
            "start_pos": 99
        }
    ], 
    "crossref": [
        {
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_612533.1", 
            "id": "NP_612533.1", 
            "database": "RefSeq"
        }, 
        {
            "url": "https://www.genome.jp/dbget-bin/www_bget?rno:171553", 
            "id": "rno:171553", 
            "database": "KEGG Pathway"
        }, 
        {
            "url": "https://bgee.org/?page=gene&gene_id=ENSRNOG00000005935", 
            "id": "ENSRNOG00000005935", 
            "database": "Bgee"
        }, 
        {
            "url": "https://omabrowser.org/oma/group/HAWHYHW/", 
            "id": "HAWHYHW", 
            "database": "OMA"
        }, 
        {
            "url": "http://www.cazy.org/fam/GT6.html", 
            "id": "GT6", 
            "database": "CAZy"
        }, 
        {
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR029044", 
            "id": "IPR029044", 
            "database": "InterPro"
        }, 
        {
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR005076", 
            "id": "IPR005076", 
            "database": "InterPro"
        }, 
        {
            "url": "http://pfam.xfam.org/family/PF03414", 
            "id": "PF03414", 
            "database": "Pfam"
        }, 
        {
            "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR10462", 
            "id": "PTHR10462", 
            "database": "PANTHER"
        }, 
        {
            "url": "http://research.bioinformatics.udel.edu/pro/entry/PR_A0A4Z3", 
            "id": "PR_A0A4Z3", 
            "database": "PRO"
        }, 
        {
            "url": "https://www.ncbi.nlm.nih.gov/gene/171553", 
            "id": "171553", 
            "database": "GeneID"
        }, 
        {
            "url": "http://rgd.mcw.edu/tools/genes/genes_view.cgi?id=727913", 
            "id": "727913", 
            "database": "RGD"
        }
    ], 
    "expression_disease": [], 
    "uniprot_ac": "A0A4Z3", 
    "disease": [], 
    "mass": {
        "monoisotopic_mass": 39548.0, 
        "chemical_mass": 39548.0
    }, 
    "expression_tissue": [], 
    "gene": [
        {
            "url": "https://www.uniprot.org/uniprot/A0A4Z3", 
            "name": "A3galt2.", 
            "locus": {
                "end_pos": 146945880, 
                "start_pos": 146933592, 
                "chromosome": "5", 
                "evidence": [
                    {
                        "url": "https://www.ensembl.org/id/ENSRNOG00000005935", 
                        "id": "ENSRNOG00000005935", 
                        "database": "Ensembl Gene"
                    }
                ]
            }
        }
    ]
}