{ "uniprot_canonical_ac": "A4Q9F1-1", "sequence": { "header": "sp|A4Q9F1-1|TTLL8_MOUSE Isoform 1 of Protein monoglycylase TTLL8 OS=Mus musculus OX=10090 GN=Ttll8 PE=1 SV=1 CANONICAL=A4Q9F1-1", "length": 832, "sequence": "MSCPPTPNPPFRPPSHTRVLRTPPLPPWVCLNSKSLSTGVGGQKNQLREASMENGERKKLSSTLSDGDHKEENKLKQGIPQDLSSSPKLDRYKIARQLTEKAIKERKIFSIYGHYPVIRATLRRKGWVEKKFNFFPKALQNLGSEDKSAETKENQEIALERFDDIHDVMSRLVKNEIPYLLWTIKRDVVDYHSLTCDQMLNHYGKTASFTTKIGLCLNMRSLPWYVQANPNTFFPRCYGLCTESEKQEFLDDFRRTVAASILKWVVLHQNYCSKVKGKSKKEEAKNSDPSPKKDPENPDLKLPSLSGQVVDTACKVCQAYLGQLEHEDIDVSEASTEALSEEEWNDLTQQYYLLVHGNASITDSKSYFAQCQALLSKISSVNPQTEIDGIRNIWIIKPAAKSRGRDIVCMDRVENILSLVAADSQTTKDNKWVVQKYIETPMLIYDTKFDIRQWFLVTDWNPLTIWFYKESYLRFSTQRFSLDKLDSAIHLCNNSIQRRLKNDKERSPLLPCHNMWTSTRFQEYLQKRGRGGTWGSIIYPSMKRAVTNAMRVAQDHVEARKNSFELYGADFILGRDFKPWLIEINSSPTMHPSTPVTAQLCAQVQEDTIKVVVDRKLDRNCDIGNFELLWRQPAVELPPFNGSDLCVEGISVKKAKKQMPPIASVGLSESLLDAPPKVRSARALMETVIRPPRTTVRQDWKREEAKVLSTTWSMPVMDAEVRGRAKPIYAFEVNDYQHVDNKSHKSGYTRVQSSKVPGVTLTSAQHPALFAQTMKPTQMTSSPPPTASGNHRDSSPFCPIVFEELWLHPNSQRRPSSCILQSRAQGWIRGIP" }, "uniprot_id": "TTLL8_MOUSE", "enzyme_annotation": [ { "ec": "6.3.2.-", "activity": "" } ], "synonyms": { "protein": { "refseq": [ "protein monoglycylase TTLL8 isoform 1", "protein monoglycylase TTLL8; TTL domain protein; tubulin--tyrosine ligase-like protein 8" ], "uniprotkb": [ "Protein monoglycylase TTLL8", "6.3.2.-", "Tubulin--tyrosine ligase-like protein 8" ] }, "gene": { "refseq": [ "Ttll8", "1700019P01Rik", "4933401B17" ], "uniprotkb": [ "Ttll8", "1700019P01Rik", "4933401B17" ] } }, "keywords": [], "species": [ { "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F1", "id": "A4Q9F1", "database": "UniProtKB" } ], "name": "Mus musculus", "taxid": 10090 } ], "recommendedname": { "full": "Protein monoglycylase TTLL8; 6.3.2.-; Tubulin--tyrosine ligase-like protein 8." }, "publication": [ { "title": "The Vertebrate Genome Annotation browser 10 years on.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/24316575", "journal": "Nucleic acids research", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_766406.2", "id": "NP_766406.2", "database": "RefSeq" } ], "authors": "Harrow JL, Steward CA, Frankish A, Gilbert JG, Gonzalez JM, Loveland JE, Mudge J, Sheppard D, Thomas M, Trevanion S, Wilming LG", "date": "2014", "pmid": "24316575" }, { "title": "Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/23897886", "journal": "The Journal of cell biology", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_766406.2", "id": "NP_766406.2", "database": "RefSeq" } ], "authors": "Bosch Grau M, Gonzalez Curto G, Rocha C, Magiera MM, Marques Sousa P, Giordano T, Spassky N, Janke C", "date": "2013", "pmid": "23897886" }, { "title": "Evolutionary divergence of enzymatic mechanisms for posttranslational polyglycylation.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/19524510", "journal": "Cell", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_766406.2", "id": "NP_766406.2", "database": "RefSeq" }, { "url": "https://www.uniprot.org/uniprot/A4Q9F1/publications", "id": "A4Q9F1", "database": "UniProtKB" } ], "authors": "Rogowski K, Juge F, van Dijk J, Wloga D, Strub JM, Levilliers N, Thomas D, Bre MH, Van Dorsselaer A, Gaertig J, Janke C", "date": "2009", "pmid": "19524510" }, { "title": "TTLL10 can perform tubulin glycylation when co-expressed with TTLL8.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/19427864", "journal": "FEBS letters", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_766406.2", "id": "NP_766406.2", "database": "RefSeq" }, { "url": "https://www.uniprot.org/uniprot/A4Q9F1/publications", "id": "A4Q9F1", "database": "UniProtKB" } ], "authors": "Ikegami K, Setou M", "date": "2009", "pmid": "19427864" }, { "title": "A targeted multienzyme mechanism for selective microtubule polyglutamylation.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/17499049", "journal": "Molecular cell", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_766406.2", "id": "NP_766406.2", "database": "RefSeq" }, { "url": "https://www.uniprot.org/uniprot/A4Q9F1/publications", "id": "A4Q9F1", "database": "UniProtKB" } ], "authors": "van Dijk J, Rogowski K, Miro J, Lacroix B, Edde B, Janke C", "date": "2007", "pmid": "17499049" } ], "glycosylation": [], "mutation": [], "isoforms": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F1#A4Q9F1-1", "isoform_ac": "A4Q9F1-1", "locus": { "end_pos": 88914035, "start_pos": 88954075, "chromosome": "15", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000109371", "id": "ENSMUST00000109371", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000104996", "id": "ENSMUSP00000104996", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "sp|A4Q9F1-1|TTLL8_MOUSE Isoform 1 of Protein monoglycylase TTLL8 OS=Mus musculus OX=10090 GN=Ttll8 PE=1 SV=1 CANONICAL=A4Q9F1-1", "length": 832, "sequence": "MSCPPTPNPPFRPPSHTRVLRTPPLPPWVCLNSKSLSTGVGGQKNQLREASMENGERKKLSSTLSDGDHKEENKLKQGIPQDLSSSPKLDRYKIARQLTEKAIKERKIFSIYGHYPVIRATLRRKGWVEKKFNFFPKALQNLGSEDKSAETKENQEIALERFDDIHDVMSRLVKNEIPYLLWTIKRDVVDYHSLTCDQMLNHYGKTASFTTKIGLCLNMRSLPWYVQANPNTFFPRCYGLCTESEKQEFLDDFRRTVAASILKWVVLHQNYCSKVKGKSKKEEAKNSDPSPKKDPENPDLKLPSLSGQVVDTACKVCQAYLGQLEHEDIDVSEASTEALSEEEWNDLTQQYYLLVHGNASITDSKSYFAQCQALLSKISSVNPQTEIDGIRNIWIIKPAAKSRGRDIVCMDRVENILSLVAADSQTTKDNKWVVQKYIETPMLIYDTKFDIRQWFLVTDWNPLTIWFYKESYLRFSTQRFSLDKLDSAIHLCNNSIQRRLKNDKERSPLLPCHNMWTSTRFQEYLQKRGRGGTWGSIIYPSMKRAVTNAMRVAQDHVEARKNSFELYGADFILGRDFKPWLIEINSSPTMHPSTPVTAQLCAQVQEDTIKVVVDRKLDRNCDIGNFELLWRQPAVELPPFNGSDLCVEGISVKKAKKQMPPIASVGLSESLLDAPPKVRSARALMETVIRPPRTTVRQDWKREEAKVLSTTWSMPVMDAEVRGRAKPIYAFEVNDYQHVDNKSHKSGYTRVQSSKVPGVTLTSAQHPALFAQTMKPTQMTSSPPPTASGNHRDSSPFCPIVFEELWLHPNSQRRPSSCILQSRAQGWIRGIP" } }, { "url": "https://www.uniprot.org/uniprot/A4Q9F1#H3BKG4-1", "isoform_ac": "H3BKG4-1", "locus": { "end_pos": 88933884, "start_pos": 88954075, "chromosome": "15", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000177180", "id": "ENSMUST00000177180", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000135381", "id": "ENSMUSP00000135381", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|H3BKG4-1|TTLL8_MOUSE Computationally mapped isoform of Protein monoglycylase TTLL8 OS=Mus musculus OX=10090 GN=Ttll8 PE=1 SV=1 CANONICAL=A4Q9F1-1", "length": 194, "sequence": "MSCPPTPNPPFRPPSHTRVLRTPPLPPWVCLNSKSLSTGVGGQKNQLREASMENGERKKLSSTLSDGDHKEENKLKQGIPQDLSSSPKLDRYKIARQLTEKAIKERKIFSIYGHYPVIRATLRRKGWVEKKFNFFPKALQNLGSEDKSAETKENQEIALERFDDIHDVMVGKERDPIPPLDYQEGCCGLPQPDL" } }, { "url": "https://www.uniprot.org/uniprot/A4Q9F1#H3BJ58-1", "isoform_ac": "H3BJ58-1", "locus": { "end_pos": 88933883, "start_pos": 88954059, "chromosome": "15", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000177254", "id": "ENSMUST00000177254", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000134858", "id": "ENSMUSP00000134858", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|H3BJ58-1|TTLL8_MOUSE Computationally mapped isoform of Protein monoglycylase TTLL8 OS=Mus musculus OX=10090 GN=Ttll8 PE=1 SV=1 CANONICAL=A4Q9F1-1", "length": 189, "sequence": "XPNPPFRPPSHTRVLRTPPLPPWVCLNSKSLSTGVGGQKNQLREASMENGERKKLSSTLSDGDHKEENKLKQGIPQDLSSSPKLDRYKIARQLTEKAIKERKIFSIYGHYPVIRATLRRKGWVEKKFNFFPKALQNLGSEDKSAETKENQEIALERFDDIHDVMVGKERDPIPPLDYQEGCCGLPQPDL" } } ], "pathway": [ { "url": "https://www.genome.jp/dbget-bin/www_bget?mmu:239591", "resource": "KEGG Pathway", "id": "mmu:239591", "name": "" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-8955332", "resource": "Reactome", "id": "R-MMU-8955332", "name": "Carboxyterminal post-translational modifications of tubulin" } ], "function": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F1#function", "annotation": "Monoglycylase which modifies both tubulin and non-tubulin proteins, generating side chains of glycine on the gamma-carboxyl groups of specific glutamate residues of target proteins. Monoglycylates tubulin, with a preference for alpha-tubulin toward beta-tubulin. Has the ability to modify non-tubulin proteins such as ANP32A, ANP32B, SET and NCL. Involved in the side-chain initiation step of the glycylation reaction by adding a single glycine chain to generate monoglycine side chains. Not involved in elongation step of the polyglycylation reaction.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F1#function", "id": "A4Q9F1", "database": "UniProtKB" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_766406.2", "annotation": "The TTLL8 was required for stability and maintenance of ependymal cilia, whereas the polyglutamylase TTLL6 was necessary for coordinated beating behavior.", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_766406.2", "id": "NP_766406.2", "database": "RefSeq" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_766406.2", "annotation": "TTLL10 can strongly glycylate tubulin in a TTLL8-dependent manner. Furthermore, these two TTLL proteins can glycylate unidentified 170-, 110-, 75-, 40-, 35-, and 30-kDa acidic proteins.", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_766406.2", "id": "NP_766406.2", "database": "RefSeq" } ] } ], "orthologs": [], "refseq": { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_766406.2", "ac": "NP_766406.2", "name": "protein monoglycylase TTLL8 isoform 1; protein monoglycylase TTLL8; TTL domain protein; tubulin--tyrosine ligase-like protein 8.", "summary": "" }, "go_annotation": { "categories": [ { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0070735", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F1", "id": "A4Q9F1", "database": "UniProtKB" } ], "name": "protein-glycine ligase activity", "id": "GO:0070735" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0070736", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F1", "id": "A4Q9F1", "database": "UniProtKB" } ], "name": "protein-glycine ligase activity, initiating", "id": "GO:0070736" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005524", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F1", "id": "A4Q9F1", "database": "UniProtKB" } ], "name": "ATP binding", "id": "GO:0005524" } ], "total": 3, "name": "Molecular Function" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005929", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F1", "id": "A4Q9F1", "database": "UniProtKB" } ], "name": "cilium", "id": "GO:0005929" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0015630", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F1", "id": "A4Q9F1", "database": "UniProtKB" } ], "name": "microtubule cytoskeleton", "id": "GO:0015630" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005930", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F1", "id": "A4Q9F1", "database": "UniProtKB" } ], "name": "axoneme", "id": "GO:0005930" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005874", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F1", "id": "A4Q9F1", "database": "UniProtKB" } ], "name": "microtubule", "id": "GO:0005874" } ], "total": 4, "name": "Cellular Component" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0060271", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F1", "id": "A4Q9F1", "database": "UniProtKB" } ], "name": "cilium assembly", "id": "GO:0060271" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0018094", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F1", "id": "A4Q9F1", "database": "UniProtKB" } ], "name": "protein polyglycylation", "id": "GO:0018094" } ], "total": 2, "name": "Biological Process" } ] }, "site_annotation": [ { "end_pos": 360, "annotation": "n_glycosylation_sequon", "start_pos": 358 }, { "end_pos": 495, "annotation": "n_glycosylation_sequon", "start_pos": 493 }, { "end_pos": 587, "annotation": "n_glycosylation_sequon", "start_pos": 585 }, { "end_pos": 643, "annotation": "n_glycosylation_sequon", "start_pos": 641 }, { "end_pos": 743, "annotation": "n_glycosylation_sequon", "start_pos": 741 } ], "crossref": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_766406.2", "id": "NP_766406.2", "database": "RefSeq" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-8955332", "id": "R-MMU-8955332", "database": "Reactome" }, { "url": "https://www.genome.jp/dbget-bin/www_bget?mmu:239591", "id": "mmu:239591", "database": "KEGG Pathway" }, { "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000022388", "id": "ENSMUSG00000022388", "database": "Bgee" }, { "url": "https://omabrowser.org/oma/group/VDIACKV/", "id": "VDIACKV", "database": "OMA" }, { "url": "https://www.ebi.ac.uk/interpro/entry/IPR004344", "id": "IPR004344", "database": "InterPro" }, { "url": "http://pfam.xfam.org/family/PF03133", "id": "PF03133", "database": "Pfam" }, { "url": "http://research.bioinformatics.udel.edu/pro/entry/PR_A4Q9F1", "id": "PR_A4Q9F1", "database": "PRO" }, { "url": "https://www.ncbi.nlm.nih.gov/gene/239591", "id": "239591", "database": "GeneID" }, { "url": "http://www.informatics.jax.org/marker/MGI:1922902", "id": "1922902", "database": "MGI" } ], "expression_disease": [], "uniprot_ac": "A4Q9F1", "disease": [], "mass": { "monoisotopic_mass": 94915.0, "chemical_mass": 94915.0 }, "expression_tissue": [], "gene": [ { "url": "https://www.uniprot.org/uniprot/A4Q9F1", "name": "Ttll8; 1700019P01Rik; 4933401B17.", "locus": { "end_pos": 88890633, "start_pos": 88954418, "chromosome": "15", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUSG00000022388", "id": "ENSMUSG00000022388", "database": "Ensembl Gene" } ] } } ] }