{
    "uniprot_canonical_ac": "A3QJZ6-1", 
    "sequence": {
        "header": "sp|A3QJZ6-1|PRA22_HUMAN Isoform 1 of PRAME family member 22  OS=Homo sapiens OX=9606 GN=PRAMEF22 PE=3 SV=3 CANONICAL=A3QJZ6-1", 
        "length": 481, 
        "sequence": "MRMSLQAPRRLLELAGQSLLGDQALAISILDELPRELFPPLFVEAFTSRRCEVLKVMVQAWPFPCLPLGSLMKTPDLEILHYVVDGIDCLLAQKVRPRRWKLQVLELRDVDENFWTIWSGARPLSCSPEAMSKRQTVEDCPRTGEKQPLKVFMDVCLKEKFMDEDLSFFSGWVQHRRGSVHLCCTKVVNYSMSILNFRNILETVYPDSIQVLEIWNMCWPCMIVEFSRYLSQMRNLRKLFISDGCRYLLSSDSQEQLVAEFSSVLLRLEYLQMLYVRRVCFFRGHLDQLIRCLRSPLETLALTYGFLEKVDLKCLPRYPSLSQLKQLNLSHGALRFIRLEPLRALLEKVAATLQTLFLVDCGIRDSKLRVILPALSCCSNLTTFCFHGNDTSMDGLKDLLRHTGRLSNLSLETYPAPRESLDDRGRVISELLTPLQAELMRILREVREPKRIFFGPVSCPCCGTSPTEQLEFNFCLWGRPA"
    }, 
    "uniprot_id": "PRA22_HUMAN", 
    "enzyme_annotation": [], 
    "synonyms": {
        "protein": {
            "refseq": [
                "PRAME family member 22"
            ], 
            "uniprotkb": [
                "PRAME family member 22"
            ]
        }, 
        "gene": {
            "refseq": [], 
            "uniprotkb": [
                "PRAMEF22"
            ]
        }
    }, 
    "keywords": [], 
    "species": [
        {
            "evidence": [
                {
                    "url": "https://www.uniprot.org/uniprot/A3QJZ6", 
                    "id": "A3QJZ6", 
                    "database": "UniProtKB"
                }
            ], 
            "name": "Homo sapiens", 
            "taxid": 9606
        }
    ], 
    "recommendedname": {
        "full": "PRAME family member 22."
    }, 
    "publication": [], 
    "glycosylation": [], 
    "mutation": [
        {
            "start_pos": 472, 
            "subjects_positive": "4", 
            "evidence": [
                {
                    "url": "https://hive.biochemistry.gwu.edu/biomuta/proteinview/A3QJZ6", 
                    "id": "A3QJZ6", 
                    "database": "BioMuta"
                }
            ], 
            "frequency": "3.2", 
            "annotation": "Filters passed (1/5): patient freq. (3.2%).", 
            "sequence_org": "F", 
            "disease": {
                "doid": "2531", 
                "url": "http://disease-ontology.org/term/DOID:2531", 
                "icd10": "", 
                "name": "Hematologic cancer"
            }, 
            "sequence_mut": "S", 
            "subjects_tested": "125", 
            "end_pos": 472, 
            "type": "Point mutation"
        }, 
        {
            "start_pos": 423, 
            "subjects_positive": "1", 
            "evidence": [
                {
                    "url": "https://hive.biochemistry.gwu.edu/biomuta/proteinview/A3QJZ6", 
                    "id": "A3QJZ6", 
                    "database": "BioMuta"
                }
            ], 
            "frequency": "1.5", 
            "annotation": "Filters passed (1/5): patient freq. (1.5%).", 
            "sequence_org": "D", 
            "disease": {
                "doid": "1324", 
                "url": "http://disease-ontology.org/term/DOID:1324", 
                "icd10": "", 
                "name": "Lung cancer"
            }, 
            "sequence_mut": "N", 
            "subjects_tested": "66", 
            "end_pos": 423, 
            "type": "Point mutation"
        }, 
        {
            "start_pos": 119, 
            "subjects_positive": "1", 
            "evidence": [
                {
                    "url": "https://hive.biochemistry.gwu.edu/biomuta/proteinview/A3QJZ6", 
                    "id": "A3QJZ6", 
                    "database": "BioMuta"
                }
            ], 
            "frequency": "1.1", 
            "annotation": "Filters passed (1/5): patient freq. (1.1%).", 
            "sequence_org": "S", 
            "disease": {
                "doid": "9256", 
                "url": "http://disease-ontology.org/term/DOID:9256", 
                "icd10": "", 
                "name": "Colorectal cancer"
            }, 
            "sequence_mut": "C", 
            "subjects_tested": "94", 
            "end_pos": 119, 
            "type": "Point mutation"
        }, 
        {
            "start_pos": 237, 
            "subjects_positive": "1", 
            "evidence": [
                {
                    "url": "https://hive.biochemistry.gwu.edu/biomuta/proteinview/A3QJZ6", 
                    "id": "A3QJZ6", 
                    "database": "BioMuta"
                }
            ], 
            "frequency": "0.2", 
            "annotation": "Filters passed (1/5): num. of cancers (4).", 
            "sequence_org": "R", 
            "disease": {
                "doid": "9256", 
                "url": "http://disease-ontology.org/term/DOID:9256", 
                "icd10": "", 
                "name": "Colorectal cancer"
            }, 
            "sequence_mut": "H", 
            "subjects_tested": "456", 
            "end_pos": 237, 
            "type": "Point mutation"
        }, 
        {
            "start_pos": 237, 
            "subjects_positive": "1", 
            "evidence": [
                {
                    "url": "https://hive.biochemistry.gwu.edu/biomuta/proteinview/A3QJZ6", 
                    "id": "A3QJZ6", 
                    "database": "BioMuta"
                }
            ], 
            "frequency": "0.1", 
            "annotation": "Filters passed (1/5): num. of cancers (4).", 
            "sequence_org": "R", 
            "disease": {
                "doid": "10534", 
                "url": "http://disease-ontology.org/term/DOID:10534", 
                "icd10": "", 
                "name": "Stomach cancer"
            }, 
            "sequence_mut": "H", 
            "subjects_tested": "1044", 
            "end_pos": 237, 
            "type": "Point mutation"
        }, 
        {
            "start_pos": 309, 
            "subjects_positive": "1", 
            "evidence": [
                {
                    "url": "https://hive.biochemistry.gwu.edu/biomuta/proteinview/A3QJZ6", 
                    "id": "A3QJZ6", 
                    "database": "BioMuta"
                }
            ], 
            "frequency": "1.5", 
            "annotation": "Filters passed (1/5): patient freq. (1.5%).", 
            "sequence_org": "K", 
            "disease": {
                "doid": "1324", 
                "url": "http://disease-ontology.org/term/DOID:1324", 
                "icd10": "", 
                "name": "Lung cancer"
            }, 
            "sequence_mut": "E", 
            "subjects_tested": "66", 
            "end_pos": 309, 
            "type": "Point mutation"
        }, 
        {
            "start_pos": 237, 
            "subjects_positive": "1", 
            "evidence": [
                {
                    "url": "https://hive.biochemistry.gwu.edu/biomuta/proteinview/A3QJZ6", 
                    "id": "A3QJZ6", 
                    "database": "BioMuta"
                }
            ], 
            "frequency": "0.2", 
            "annotation": "Filters passed (1/5): num. of cancers (4).", 
            "sequence_org": "R", 
            "disease": {
                "doid": "363", 
                "url": "http://disease-ontology.org/term/DOID:363", 
                "icd10": "", 
                "name": "Uterine cancer"
            }, 
            "sequence_mut": "H", 
            "subjects_tested": "558", 
            "end_pos": 237, 
            "type": "Point mutation"
        }, 
        {
            "start_pos": 50, 
            "subjects_positive": "1", 
            "evidence": [
                {
                    "url": "https://hive.biochemistry.gwu.edu/biomuta/proteinview/A3QJZ6", 
                    "id": "A3QJZ6", 
                    "database": "BioMuta"
                }
            ], 
            "frequency": "2.0", 
            "annotation": "Filters passed (1/5): patient freq. (2.0%).", 
            "sequence_org": "R", 
            "disease": {
                "doid": "1781", 
                "url": "http://disease-ontology.org/term/DOID:1781", 
                "icd10": "", 
                "name": "Thyroid cancer"
            }, 
            "sequence_mut": "C", 
            "subjects_tested": "50", 
            "end_pos": 50, 
            "type": "Point mutation"
        }, 
        {
            "start_pos": 237, 
            "subjects_positive": "1", 
            "evidence": [
                {
                    "url": "https://hive.biochemistry.gwu.edu/biomuta/proteinview/A3QJZ6", 
                    "id": "A3QJZ6", 
                    "database": "BioMuta"
                }
            ], 
            "frequency": "0.1", 
            "annotation": "Filters passed (1/5): num. of cancers (4).", 
            "sequence_org": "R", 
            "disease": {
                "doid": "1324", 
                "url": "http://disease-ontology.org/term/DOID:1324", 
                "icd10": "", 
                "name": "Lung cancer"
            }, 
            "sequence_mut": "H", 
            "subjects_tested": "689", 
            "end_pos": 237, 
            "type": "Point mutation"
        }, 
        {
            "start_pos": 204, 
            "subjects_positive": "2", 
            "evidence": [
                {
                    "url": "https://hive.biochemistry.gwu.edu/biomuta/proteinview/A3QJZ6", 
                    "id": "A3QJZ6", 
                    "database": "BioMuta"
                }
            ], 
            "frequency": "4.0", 
            "annotation": "Filters passed (1/5): patient freq. (4.0%).", 
            "sequence_org": "V", 
            "disease": {
                "doid": "1781", 
                "url": "http://disease-ontology.org/term/DOID:1781", 
                "icd10": "", 
                "name": "Thyroid cancer"
            }, 
            "sequence_mut": "I", 
            "subjects_tested": "50", 
            "end_pos": 204, 
            "type": "Point mutation"
        }, 
        {
            "start_pos": 310, 
            "subjects_positive": "1", 
            "evidence": [
                {
                    "url": "https://hive.biochemistry.gwu.edu/biomuta/proteinview/A3QJZ6", 
                    "id": "A3QJZ6", 
                    "database": "BioMuta"
                }
            ], 
            "frequency": "1.5", 
            "annotation": "Filters passed (1/5): patient freq. (1.5%).", 
            "sequence_org": "V", 
            "disease": {
                "doid": "1324", 
                "url": "http://disease-ontology.org/term/DOID:1324", 
                "icd10": "", 
                "name": "Lung cancer"
            }, 
            "sequence_mut": "E", 
            "subjects_tested": "66", 
            "end_pos": 310, 
            "type": "Point mutation"
        }, 
        {
            "start_pos": 333, 
            "subjects_positive": "1", 
            "evidence": [
                {
                    "url": "https://hive.biochemistry.gwu.edu/biomuta/proteinview/A3QJZ6", 
                    "id": "A3QJZ6", 
                    "database": "BioMuta"
                }
            ], 
            "frequency": "2.0", 
            "annotation": "Filters passed (1/5): patient freq. (2.0%).", 
            "sequence_org": "A", 
            "disease": {
                "doid": "1781", 
                "url": "http://disease-ontology.org/term/DOID:1781", 
                "icd10": "", 
                "name": "Thyroid cancer"
            }, 
            "sequence_mut": "T", 
            "subjects_tested": "50", 
            "end_pos": 333, 
            "type": "Point mutation"
        }, 
        {
            "start_pos": 377, 
            "subjects_positive": "2", 
            "evidence": [
                {
                    "url": "https://hive.biochemistry.gwu.edu/biomuta/proteinview/A3QJZ6", 
                    "id": "A3QJZ6", 
                    "database": "BioMuta"
                }
            ], 
            "frequency": "3.0", 
            "annotation": "Filters passed (1/5): patient freq. (3.0%).", 
            "sequence_org": "C", 
            "disease": {
                "doid": "1324", 
                "url": "http://disease-ontology.org/term/DOID:1324", 
                "icd10": "", 
                "name": "Lung cancer"
            }, 
            "sequence_mut": "R", 
            "subjects_tested": "66", 
            "end_pos": 377, 
            "type": "Point mutation"
        }
    ], 
    "isoforms": [
        {
            "url": "https://www.uniprot.org/uniprot/A3QJZ6#A3QJZ6-1", 
            "isoform_ac": "A3QJZ6-1", 
            "locus": {
                "end_pos": 0, 
                "start_pos": 0, 
                "chromosome": "", 
                "evidence": []
            }, 
            "sequence": {
                "header": "sp|A3QJZ6-1|PRA22_HUMAN Isoform 1 of PRAME family member 22  OS=Homo sapiens OX=9606 GN=PRAMEF22 PE=3 SV=3 CANONICAL=A3QJZ6-1", 
                "length": 481, 
                "sequence": "MRMSLQAPRRLLELAGQSLLGDQALAISILDELPRELFPPLFVEAFTSRRCEVLKVMVQAWPFPCLPLGSLMKTPDLEILHYVVDGIDCLLAQKVRPRRWKLQVLELRDVDENFWTIWSGARPLSCSPEAMSKRQTVEDCPRTGEKQPLKVFMDVCLKEKFMDEDLSFFSGWVQHRRGSVHLCCTKVVNYSMSILNFRNILETVYPDSIQVLEIWNMCWPCMIVEFSRYLSQMRNLRKLFISDGCRYLLSSDSQEQLVAEFSSVLLRLEYLQMLYVRRVCFFRGHLDQLIRCLRSPLETLALTYGFLEKVDLKCLPRYPSLSQLKQLNLSHGALRFIRLEPLRALLEKVAATLQTLFLVDCGIRDSKLRVILPALSCCSNLTTFCFHGNDTSMDGLKDLLRHTGRLSNLSLETYPAPRESLDDRGRVISELLTPLQAELMRILREVREPKRIFFGPVSCPCCGTSPTEQLEFNFCLWGRPA"
            }
        }
    ], 
    "pathway": [
        {
            "url": "https://www.genome.jp/dbget-bin/www_bget?hsa:653606", 
            "resource": "KEGG Pathway", 
            "id": "hsa:653606", 
            "name": ""
        }
    ], 
    "function": [], 
    "orthologs": [], 
    "refseq": {
        "url": "", 
        "ac": "", 
        "name": "PRAME family member 22.", 
        "summary": ""
    }, 
    "go_annotation": {
        "categories": [
            {
                "go_terms": [
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005737", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A3QJZ6", 
                                "id": "A3QJZ6", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "cytoplasm", 
                        "id": "GO:0005737"
                    }
                ], 
                "total": 1, 
                "name": "Cellular Component"
            }, 
            {
                "go_terms": [
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0008284", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A3QJZ6", 
                                "id": "A3QJZ6", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "positive regulation of cell population proliferation", 
                        "id": "GO:0008284"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0043066", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A3QJZ6", 
                                "id": "A3QJZ6", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "negative regulation of apoptotic process", 
                        "id": "GO:0043066"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0045892", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A3QJZ6", 
                                "id": "A3QJZ6", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "negative regulation of transcription, DNA-templated", 
                        "id": "GO:0045892"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0045596", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A3QJZ6", 
                                "id": "A3QJZ6", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "negative regulation of cell differentiation", 
                        "id": "GO:0045596"
                    }
                ], 
                "total": 4, 
                "name": "Biological Process"
            }
        ]
    }, 
    "site_annotation": [
        {
            "end_pos": 191, 
            "annotation": "n_glycosylation_sequon", 
            "start_pos": 189
        }, 
        {
            "end_pos": 330, 
            "annotation": "n_glycosylation_sequon", 
            "start_pos": 328
        }, 
        {
            "end_pos": 382, 
            "annotation": "n_glycosylation_sequon", 
            "start_pos": 380
        }, 
        {
            "end_pos": 391, 
            "annotation": "n_glycosylation_sequon", 
            "start_pos": 389
        }, 
        {
            "end_pos": 410, 
            "annotation": "n_glycosylation_sequon", 
            "start_pos": 408
        }
    ], 
    "crossref": [
        {
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001094101.1", 
            "id": "NP_001094101.1", 
            "database": "RefSeq"
        }, 
        {
            "url": "https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=34393", 
            "id": "34393", 
            "database": "HGNC"
        }, 
        {
            "url": "https://pharos.nih.gov/targets/A3QJZ6", 
            "id": "A3QJZ6", 
            "database": "PHAROS"
        }, 
        {
            "url": "https://www.nextprot.org/entry/NX_A3QJZ6", 
            "id": "NX_A3QJZ6", 
            "database": "neXtProt"
        }, 
        {
            "url": "http://www.genecards.org/cgi-bin/carddisp.pl?gene=PRAMEF22", 
            "id": "PRAMEF22", 
            "database": "GeneCards"
        }, 
        {
            "url": "https://www.genome.jp/dbget-bin/www_bget?hsa:653606", 
            "id": "hsa:653606", 
            "database": "KEGG Pathway"
        }, 
        {
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR032675", 
            "id": "IPR032675", 
            "database": "InterPro"
        }, 
        {
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR026271", 
            "id": "IPR026271", 
            "database": "InterPro"
        }, 
        {
            "url": "http://research.bioinformatics.udel.edu/pro/entry/PR_A3QJZ6", 
            "id": "PR_A3QJZ6", 
            "database": "PRO"
        }, 
        {
            "url": "https://www.ncbi.nlm.nih.gov/gene/653606", 
            "id": "653606", 
            "database": "GeneID"
        }
    ], 
    "expression_disease": [], 
    "uniprot_ac": "A3QJZ6", 
    "disease": [], 
    "mass": {
        "monoisotopic_mass": 55489.0, 
        "chemical_mass": 55489.0
    }, 
    "expression_tissue": [], 
    "gene": [
        {
            "url": "https://www.uniprot.org/uniprot/A3QJZ6", 
            "name": "PRAMEF22.", 
            "locus": {
                "end_pos": 0, 
                "start_pos": 0, 
                "chromosome": "0", 
                "evidence": []
            }
        }
    ]
}