{
    "uniprot_canonical_ac": "A2AKM2-1", 
    "sequence": {
        "header": "sp|A2AKM2-1|HACD4_MOUSE Isoform 1 of Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4  OS=Mus musculus OX=10090 GN=Hacd4 PE=2 SV=2 CANONICAL=A2AKM2-1", 
        "length": 232, 
        "sequence": "MGPSVLPAWLQPRYRKNVYLFIYYLIQFCGHSWILANMTVRFFSFGKDSMADTFYAIGLVMRVCQSISLLELLHIYIGIESNQLFPRFLQLTERVIILFGVITSQEEVQEKCVVCVLFILWNLLDMVRYTYSMLSVIGTSYAALTWLSQTLWMPIYPLCVLAEAFTIYQSLPYFESFGTNSTVLPFDLSTCFPYVLKLYLMMLFIGMYFTYSHLYTERKDFLRVFSVKQKNV"
    }, 
    "uniprot_id": "HACD4_MOUSE", 
    "enzyme_annotation": [
        {
            "ec": "4.2.1.134", 
            "activity": "A very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain trans-2,3-dehydroacyl-CoA + H(2)O."
        }, 
        {
            "ec": "4.2.1.134", 
            "activity": ""
        }
    ], 
    "synonyms": {
        "protein": {
            "refseq": [
                "very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4", 
                "protein tyrosine phosphatase-like protein PTPLAD2; protein-tyrosine phosphatase-like A domain-containing protein 2; very-long-chain (3R)-3-hydroxyacyl-[acyl-carrier protein] dehydratase 4; protein tyrosine phosphatase-like A domain containing 2"
            ], 
            "uniprotkb": [
                "Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4", 
                "4.2.1.134", 
                "3-hydroxyacyl-CoA dehydratase 4", 
                "HACD4", 
                "Protein-tyrosine phosphatase-like A domain-containing protein 2"
            ]
        }, 
        "gene": {
            "refseq": [
                "Hacd4", 
                "4933428I03Rik", 
                "Hcad4", 
                "Ptplad2"
            ], 
            "uniprotkb": [
                "Hacd4", 
                "Ptplad2", 
                "4933428I03Rik", 
                "Hcad4"
            ]
        }
    }, 
    "keywords": [], 
    "species": [
        {
            "evidence": [
                {
                    "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                    "id": "A2AKM2", 
                    "database": "UniProtKB"
                }
            ], 
            "name": "Mus musculus", 
            "taxid": 10090
        }
    ], 
    "recommendedname": {
        "full": "Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4; 4.2.1.134; 3-hydroxyacyl-CoA dehydratase 4; HACD4; Protein-tyrosine phosphatase-like A domain-containing protein 2."
    }, 
    "publication": [
        {
            "title": "HACD1, a regulator of membrane composition and fluidity, promotes myoblast fusion and skeletal muscle growth.", 
            "url": "https://www.ncbi.nlm.nih.gov/pubmed/26160855", 
            "journal": "Journal of molecular cell biology", 
            "evidence": [
                {
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_080036.1", 
                    "id": "NP_080036.1", 
                    "database": "RefSeq"
                }
            ], 
            "authors": "Blondelle J, Ohno Y, Gache V, Guyot S, Storck S, Blanchard-Gutton N, Barthelemy I, Walmsley G, Rahier A, Gadin S, Maurer M, Guillaud L, Prola A, Ferry A, Aubin-Houzelstein G, Demarquoy J, Relaix F, Piercy RJ, Blot S, Kihara A, Tiret L, Pilot-Storck F", 
            "date": "2015", 
            "pmid": "26160855"
        }
    ], 
    "glycosylation": [], 
    "mutation": [], 
    "isoforms": [
        {
            "url": "https://www.uniprot.org/uniprot/A2AKM2#A2AKM2-1", 
            "isoform_ac": "A2AKM2-1", 
            "locus": {
                "end_pos": 88414267, 
                "start_pos": 88438870, 
                "chromosome": "4", 
                "evidence": [
                    {
                        "url": "https://www.ensembl.org/id/ENSMUST00000030221", 
                        "id": "ENSMUST00000030221", 
                        "database": "Ensembl Transcript"
                    }, 
                    {
                        "url": "https://www.ensembl.org/id/ENSMUSP00000030221", 
                        "id": "ENSMUSP00000030221", 
                        "database": "Ensembl Peptide"
                    }
                ]
            }, 
            "sequence": {
                "header": "sp|A2AKM2-1|HACD4_MOUSE Isoform 1 of Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4  OS=Mus musculus OX=10090 GN=Hacd4 PE=2 SV=2 CANONICAL=A2AKM2-1", 
                "length": 232, 
                "sequence": "MGPSVLPAWLQPRYRKNVYLFIYYLIQFCGHSWILANMTVRFFSFGKDSMADTFYAIGLVMRVCQSISLLELLHIYIGIESNQLFPRFLQLTERVIILFGVITSQEEVQEKCVVCVLFILWNLLDMVRYTYSMLSVIGTSYAALTWLSQTLWMPIYPLCVLAEAFTIYQSLPYFESFGTNSTVLPFDLSTCFPYVLKLYLMMLFIGMYFTYSHLYTERKDFLRVFSVKQKNV"
            }
        }, 
        {
            "url": "https://www.uniprot.org/uniprot/A2AKM2#F7CPS1-1", 
            "isoform_ac": "F7CPS1-1", 
            "locus": {
                "end_pos": 88414265, 
                "start_pos": 88438928, 
                "chromosome": "4", 
                "evidence": [
                    {
                        "url": "https://www.ensembl.org/id/ENSMUST00000151280", 
                        "id": "ENSMUST00000151280", 
                        "database": "Ensembl Transcript"
                    }, 
                    {
                        "url": "https://www.ensembl.org/id/ENSMUSP00000122565", 
                        "id": "ENSMUSP00000122565", 
                        "database": "Ensembl Peptide"
                    }
                ]
            }, 
            "sequence": {
                "header": "tr|F7CPS1-1|HACD4_MOUSE Computationally mapped isoform of Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4  OS=Mus musculus OX=10090 GN=Hacd4 PE=2 SV=1 CANONICAL=A2AKM2-1", 
                "length": 252, 
                "sequence": "XLSQPTPAASRRCQRSRLWPMGPSVLPAWLQPRYRKNVYLFIYYLIQFCGHSWILANMTVRFFSFGKDSMADTFYAIGLVMRVCQSISLLELLHIYIGIESNQLFPRFLQLTERVIILFGVITSQEEVQEKCVVCVLFILWNLLDMVRYTYSMLSVIGTSYAALTWLSQTLWMPIYPLCVLAEAFTIYQSLPYFESFGTNSTVLPFDLSTCFPYVLKLYLMMLFIGMYFTYSHLYTERKDFLRVFSVKQKNV"
            }
        }, 
        {
            "url": "https://www.uniprot.org/uniprot/A2AKM2#A2AKM2-2", 
            "isoform_ac": "A2AKM2-2", 
            "locus": {
                "end_pos": 0, 
                "start_pos": 0, 
                "chromosome": "", 
                "evidence": []
            }, 
            "sequence": {
                "header": "sp|A2AKM2-2|HACD4_MOUSE Isoform 2 of Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4  OS=Mus musculus OX=10090 GN=Hacd4 PE=2 SV=2 CANONICAL=A2AKM2-1", 
                "length": 247, 
                "sequence": "MGPSVLPAWLQPRYRKNVYLFIYYLIQFCGHSWILANMTVRFFSFGKDSMADTFYAIGLVMRVCQSISLLELLHIYIGIESNQLFPRFLQLTERVIILFGVITSQEEVQEKCVVCVLFILWNLLDMVRYTYSMLSVIGTSYAALTWLSQTLWMPIYPLCVLAEAFTIYQSLPYFESFGTNSTVLPFDLSTCFPYVLKLYLMMLFIGNAKMFLIKCKKLCLKVFHSPIHLLHVLNTGHTTRPTTRPDI"
            }
        }, 
        {
            "url": "https://www.uniprot.org/uniprot/A2AKM2#Z4YMP4-1", 
            "isoform_ac": "Z4YMP4-1", 
            "locus": {
                "end_pos": 88398095, 
                "start_pos": 88438915, 
                "chromosome": "4", 
                "evidence": [
                    {
                        "url": "https://www.ensembl.org/id/ENSMUST00000132493", 
                        "id": "ENSMUST00000132493", 
                        "database": "Ensembl Transcript"
                    }, 
                    {
                        "url": "https://www.ensembl.org/id/ENSMUSP00000119411", 
                        "id": "ENSMUSP00000119411", 
                        "database": "Ensembl Peptide"
                    }
                ]
            }, 
            "sequence": {
                "header": "tr|Z4YMP4-1|HACD4_MOUSE Computationally mapped isoform of Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4  OS=Mus musculus OX=10090 GN=Hacd4 PE=2 SV=1 CANONICAL=A2AKM2-1", 
                "length": 262, 
                "sequence": "TPAASRRCQRSRLWPMGPSVLPAWLQPRYRKNVYLFIYYLIQFCGHSWILANMTVRFFSFGKDSMADTFYAIGLVMRVCQSISLLELLHIYIGIESNQLFPRFLQLTERVIILFGVITSQEEVQEKCVVCVLFILWNLLDMVRYTYSMLSVIGTSYAALTWLSQTLWMPIYPLCVLAEAFTIYQSLPYFESFGTNSTVLPFDLSTCFPYVLKLYLMMLFIGNAKMFLIKCKKLCLKVFHSPIHLLHVLNTGHTTRPTTRPDI"
            }
        }
    ], 
    "pathway": [
        {
            "url": "https://www.genome.jp/dbget-bin/www_bget?mmu:66775", 
            "resource": "KEGG Pathway", 
            "id": "mmu:66775", 
            "name": ""
        }, 
        {
            "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-75876", 
            "resource": "Reactome", 
            "id": "R-MMU-75876", 
            "name": "Synthesis of very long-chain fatty acyl-CoAs"
        }
    ], 
    "function": [
        {
            "url": "https://www.uniprot.org/uniprot/A2AKM2#function", 
            "annotation": "Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.", 
            "evidence": [
                {
                    "url": "https://www.uniprot.org/uniprot/A2AKM2#function", 
                    "id": "A2AKM2", 
                    "database": "UniProtKB"
                }
            ]
        }
    ], 
    "orthologs": [
        {
            "uniprot_canonical_ac": "Q5VWC8-1", 
            "protein_name": "Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4; 4.2.1.134; 3-hydroxyacyl-CoA dehydratase 4; HACD4; Protein-tyrosine phosphatase-like A domain-containing protein 2.", 
            "sequence": {
                "length": 232, 
                "sequence": "MGPLALPAWLQPRYRKNAYLFIYYLIQFCGHSWIFTNMTVRFFSFGKDSMVDTFYAIGLVMRLCQSVSLLELLHIYVGIESNHLLPRFLQLTERIIILFVVITSQEEVQEKYVVCVLFVFWNLLDMVRYTYSMLSVIGISYAVLTWLSQTLWMPIYPLCVLAEAFAIYQSLPYFESFGTYSTKLPFDLSIYFPYVLKIYLMMLFIGMYFTYSHLYSERRDILGIFPIKKKKM"
            }, 
            "evidence": [
                {
                    "url": "http://www.informatics.jax.org/homology/12033", 
                    "id": "12033", 
                    "database": "MGI"
                }, 
                {
                    "url": "https://omabrowser.org/oma/group/A2AKM2/", 
                    "id": "A2AKM2", 
                    "database": "OMA"
                }
            ], 
            "organism": "Homo sapiens", 
            "tax_id": 9606
        }, 
        {
            "uniprot_canonical_ac": "D3Z8V6-1", 
            "protein_name": "Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase; 4.2.1.134.", 
            "sequence": {
                "length": 232, 
                "sequence": "MGPSVLPAWLQPRYRKSAYLFIYYLIQFCGHSWILTNMTVRFFSFGKDSMVDTFYAIGLVMRVCQSISLLELLHIYIGIESNQLFPKFLQLTERIIILFGVITSQEEVQEKYVVCVLFIFWNLLDMVRYTYSMLSVIGTSYAVLTWLSQTLWMPIYPLCVLAEAFTIYQSLPYFELFGKNSTMLPFDISTYFPYVLKLYLMMLFIGMYFTYSHLYTERKDILRGSSVKQKKM"
            }, 
            "evidence": [
                {
                    "url": "http://www.informatics.jax.org/homology/12033", 
                    "id": "12033", 
                    "database": "MGI"
                }, 
                {
                    "url": "https://omabrowser.org/oma/group/A2AKM2/", 
                    "id": "A2AKM2", 
                    "database": "OMA"
                }
            ], 
            "organism": "Rattus norvegicus", 
            "tax_id": 10116
        }
    ], 
    "refseq": {
        "url": "https://www.ncbi.nlm.nih.gov/protein/NP_080036.1", 
        "ac": "NP_080036.1", 
        "name": "very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4; protein tyrosine phosphatase-like protein PTPLAD2; protein-tyrosine phosphatase-like A domain-containing protein 2; very-long-chain (3R)-3-hydroxyacyl-[acyl-carrier protein] dehydratase 4; protein tyrosine phosphatase-like A domain containing 2.", 
        "summary": ""
    }, 
    "go_annotation": {
        "categories": [
            {
                "go_terms": [
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0019899", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "enzyme binding", 
                        "id": "GO:0019899"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0018812", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "3-hydroxyacyl-CoA dehydratase activity", 
                        "id": "GO:0018812"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0102158", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "very-long-chain 3-hydroxyacyl-CoA dehydratase activity", 
                        "id": "GO:0102158"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0102343", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "3-hydroxy-arachidoyl-CoA dehydratase activity", 
                        "id": "GO:0102343"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0102344", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "3-hydroxy-behenoyl-CoA dehydratase activity", 
                        "id": "GO:0102344"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0102345", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "3-hydroxy-lignoceroyl-CoA dehydratase activity", 
                        "id": "GO:0102345"
                    }
                ], 
                "total": 6, 
                "name": "Molecular Function"
            }, 
            {
                "go_terms": [
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0030176", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "integral component of endoplasmic reticulum membrane", 
                        "id": "GO:0030176"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005783", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "endoplasmic reticulum", 
                        "id": "GO:0005783"
                    }
                ], 
                "total": 2, 
                "name": "Cellular Component"
            }, 
            {
                "go_terms": [
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0030148", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "sphingolipid biosynthetic process", 
                        "id": "GO:0030148"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0030497", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "fatty acid elongation", 
                        "id": "GO:0030497"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0042761", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A2AKM2", 
                                "id": "A2AKM2", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "very long-chain fatty acid biosynthetic process", 
                        "id": "GO:0042761"
                    }
                ], 
                "total": 3, 
                "name": "Biological Process"
            }
        ]
    }, 
    "site_annotation": [
        {
            "end_pos": 39, 
            "annotation": "n_glycosylation_sequon", 
            "start_pos": 37
        }, 
        {
            "end_pos": 182, 
            "annotation": "n_glycosylation_sequon", 
            "start_pos": 180
        }
    ], 
    "crossref": [
        {
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_080036.1", 
            "id": "NP_080036.1", 
            "database": "RefSeq"
        }, 
        {
            "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-75876", 
            "id": "R-MMU-75876", 
            "database": "Reactome"
        }, 
        {
            "url": "https://www.genome.jp/dbget-bin/www_bget?mmu:66775", 
            "id": "mmu:66775", 
            "database": "KEGG Pathway"
        }, 
        {
            "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000028497", 
            "id": "ENSMUSG00000028497", 
            "database": "Bgee"
        }, 
        {
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR007482", 
            "id": "IPR007482", 
            "database": "InterPro"
        }, 
        {
            "url": "http://pfam.xfam.org/family/PF04387", 
            "id": "PF04387", 
            "database": "Pfam"
        }, 
        {
            "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR11035", 
            "id": "PTHR11035", 
            "database": "PANTHER"
        }, 
        {
            "url": "http://research.bioinformatics.udel.edu/pro/entry/PR_A2AKM2", 
            "id": "PR_A2AKM2", 
            "database": "PRO"
        }, 
        {
            "url": "https://www.ncbi.nlm.nih.gov/gene/66775", 
            "id": "66775", 
            "database": "GeneID"
        }, 
        {
            "url": "http://www.informatics.jax.org/marker/MGI:1914025", 
            "id": "1914025", 
            "database": "MGI"
        }
    ], 
    "expression_disease": [], 
    "uniprot_ac": "A2AKM2", 
    "disease": [], 
    "mass": {
        "monoisotopic_mass": 27235.0, 
        "chemical_mass": 27235.0
    }, 
    "expression_tissue": [], 
    "gene": [
        {
            "url": "https://www.uniprot.org/uniprot/A2AKM2", 
            "name": "Hacd4; Ptplad2; 4933428I03Rik; Hcad4.", 
            "locus": {
                "end_pos": 88396144, 
                "start_pos": 88438928, 
                "chromosome": "4", 
                "evidence": [
                    {
                        "url": "https://www.ensembl.org/id/ENSMUSG00000028497", 
                        "id": "ENSMUSG00000028497", 
                        "database": "Ensembl Gene"
                    }
                ]
            }
        }
    ]
}