{ "uniprot_canonical_ac": "A2A5Z6-1", "sequence": { "header": "sp|A2A5Z6-1|SMUF2_MOUSE Isoform 1 of E3 ubiquitin-protein ligase SMURF2 OS=Mus musculus OX=10090 GN=Smurf2 PE=1 SV=1 CANONICAL=A2A5Z6-1", "length": 748, "sequence": "MSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGHSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVPLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKVNTRLKHCTPDSNVVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE" }, "uniprot_id": "SMUF2_MOUSE", "enzyme_annotation": [ { "ec": "2.3.2.26", "activity": "S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine." }, { "ec": "2.3.2.26", "activity": "" } ], "synonyms": { "protein": { "refseq": [ "E3 ubiquitin-protein ligase SMURF2 isoform 1", "E3 ubiquitin-protein ligase SMURF2; SMAD ubiquitination regulatory factor 2; HECT-type E3 ubiquitin transferase SMURF2" ], "uniprotkb": [ "E3 ubiquitin-protein ligase SMURF2", "2.3.2.26", "SMAD-specific E3 ubiquitin-protein ligase 2", "SMAD ubiquitination regulatory factor 2", "HECT-type E3 ubiquitin transferase SMURF2" ] }, "gene": { "refseq": [ "Smurf2", "2810411E22Rik", "AI558114", "AI649275" ], "uniprotkb": [ "Smurf2", "2810411E22Rik", "AI558114", "AI649275" ] } }, "keywords": [], "species": [ { "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A5Z6", "id": "A2A5Z6", "database": "UniProtKB" } ], "name": "Mus musculus", "taxid": 10090 } ], "recommendedname": { "full": "E3 ubiquitin-protein ligase SMURF2; 2.3.2.26; SMAD-specific E3 ubiquitin-protein ligase 2; SMAD ubiquitination regulatory factor 2; HECT-type E3 ubiquitin transferase SMURF2." }, "publication": [ { "title": "Bmp2 and Notch cooperate to pattern the embryonic endocardium.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/29853617", "journal": "Development (Cambridge, England)", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_079757.2", "id": "NP_079757.2", "database": "RefSeq" } ], "authors": "Papoutsi T, Luna-Zurita L, Prados B, Zaffran S, de la Pompa JL", "date": "2018", "pmid": "29853617" }, { "title": "Generation of Smurf2 Conditional Knockout Mice.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/29805305", "journal": "International journal of biological sciences", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_079757.2", "id": "NP_079757.2", "database": "RefSeq" } ], "authors": "Tang Y, Tang LY, Xu X, Li C, Deng C, Zhang YE", "date": "2018", "pmid": "29805305" }, { "title": "Smurf2-Mediated Stabilization of DNA Topoisomerase IIalpha Controls Genomic Integrity.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/28611047", "journal": "Cancer research", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_079757.2", "id": "NP_079757.2", "database": "RefSeq" } ], "authors": "Emanuelli A, Borroni AP, Apel-Sarid L, Shah PA, Ayyathan DM, Koganti P, Levy-Cohen G, Blank M", "date": "2017", "pmid": "28611047" }, { "title": "SMURF2 regulates bone homeostasis by disrupting SMAD3 interaction with vitamin D receptor in osteoblasts.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/28216630", "journal": "Nature communications", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_079757.2", "id": "NP_079757.2", "database": "RefSeq" } ], "authors": "Xu Z, Greenblatt MB, Yan G, Feng H, Sun J, Lotinun S, Brady N, Baron R, Glimcher LH, Zou W", "date": "2017", "pmid": "28216630" }, { "title": "USP4 inhibits SMAD4 monoubiquitination and promotes activin and BMP signaling.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/28468752", "journal": "The EMBO journal", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_079757.2", "id": "NP_079757.2", "database": "RefSeq" } ], "authors": "Zhou F, Xie F, Jin K, Zhang Z, Clerici M, Gao R, van Dinther M, Sixma TK, Huang H, Zhang L, Ten Dijke P", "date": "2017", "pmid": "28468752" }, { "title": "AIMP1/p43 downregulates TGF-beta signaling via stabilization of smurf2.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/18448069", "journal": "Biochemical and biophysical research communications", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_079757.2", "id": "NP_079757.2", "database": "RefSeq" }, { "url": "https://www.uniprot.org/uniprot/A2A5Z6/publications", "id": "A2A5Z6", "database": "UniProtKB" } ], "authors": "Lee YS, Han JM, Son SH, Choi JW, Jeon EJ, Bae SC, Park YI, Kim S", "date": "2008", "pmid": "18448069" }, { "title": "Tumor necrosis factor promotes Runx2 degradation through up-regulation of Smurf1 and Smurf2 in osteoblasts.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/16373342", "journal": "The Journal of biological chemistry", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_079757.2", "id": "NP_079757.2", "database": "RefSeq" }, { "url": "https://www.uniprot.org/uniprot/A2A5Z6/publications", "id": "A2A5Z6", "database": "UniProtKB" } ], "authors": "Kaneki H, Guo R, Chen D, Yao Z, Schwarz EM, Zhang YE, Boyce BF, Xing L", "date": "2006", "pmid": "16373342" }, { "title": "Ubiquitin ligase Smurf1 controls osteoblast activity and bone homeostasis by targeting MEKK2 for degradation.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/15820682", "journal": "Cell", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A5Z6/publications", "id": "A2A5Z6", "database": "UniProtKB" } ], "authors": "Li C., Yamashita M., Deng C.X., Cheng S.Y., Zhang Y.E., Ying S.X., Zhang G.M.", "date": "2005", "pmid": "15820682" }, { "title": "BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/12520002", "journal": "Nucleic acids research", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_079757.2", "id": "NP_079757.2", "database": "RefSeq" } ], "authors": "Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE", "date": "2003", "pmid": "12520002" } ], "glycosylation": [], "mutation": [], "isoforms": [ { "url": "https://www.uniprot.org/uniprot/A2A5Z6#A2A5Z6-1", "isoform_ac": "A2A5Z6-1", "locus": { "end_pos": 106822668, "start_pos": 106920232, "chromosome": "11", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000092517", "id": "ENSMUST00000092517", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000090177", "id": "ENSMUSP00000090177", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "sp|A2A5Z6-1|SMUF2_MOUSE Isoform 1 of E3 ubiquitin-protein ligase SMURF2 OS=Mus musculus OX=10090 GN=Smurf2 PE=1 SV=1 CANONICAL=A2A5Z6-1", "length": 748, "sequence": "MSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGHSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVPLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKVNTRLKHCTPDSNVVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE" } }, { "url": "https://www.uniprot.org/uniprot/A2A5Z6#A0A338P709-1", "isoform_ac": "A0A338P709-1", "locus": { "end_pos": 106869105, "start_pos": 106920232, "chromosome": "11", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000139297", "id": "ENSMUST00000139297", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000156224", "id": "ENSMUSP00000156224", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "tr|A0A338P709-1|SMUF2_MOUSE Computationally mapped isoform of E3 ubiquitin-protein ligase SMURF2 OS=Mus musculus OX=10090 GN=Smurf2 PE=1 SV=1 CANONICAL=A2A5Z6-1", "length": 68, "sequence": "MSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLQ" } }, { "url": "https://www.uniprot.org/uniprot/A2A5Z6#A2A5Z6-2", "isoform_ac": "A2A5Z6-2", "locus": { "end_pos": 106822668, "start_pos": 106920232, "chromosome": "11", "evidence": [ { "url": "https://www.ensembl.org/id/ENSMUST00000103067", "id": "ENSMUST00000103067", "database": "Ensembl Transcript" }, { "url": "https://www.ensembl.org/id/ENSMUSP00000099356", "id": "ENSMUSP00000099356", "database": "Ensembl Peptide" } ] }, "sequence": { "header": "sp|A2A5Z6-2|SMUF2_MOUSE Isoform 2 of E3 ubiquitin-protein ligase SMURF2 OS=Mus musculus OX=10090 GN=Smurf2 PE=1 SV=1 CANONICAL=A2A5Z6-1", "length": 735, "sequence": "MSNPGGRRNGPVKLRLTGLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGHSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVPLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKVNTRLKHCTPDSNVVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE" } } ], "pathway": [ { "url": "https://www.genome.jp/dbget-bin/www_bget?mmu:66313", "resource": "KEGG Pathway", "id": "mmu:66313", "name": "" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-201451", "resource": "Reactome", "id": "R-MMU-201451", "name": "Signaling by BMP" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-4608870", "resource": "Reactome", "id": "R-MMU-4608870", "name": "Asymmetric localization of PCP proteins" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-983168", "resource": "Reactome", "id": "R-MMU-983168", "name": "Antigen processing: Ubiquitination & Proteasome degradation" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-2173788", "resource": "Reactome", "id": "R-MMU-2173788", "name": "Downregulation of TGF-beta receptor signaling" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-2173795", "resource": "Reactome", "id": "R-MMU-2173795", "name": "Downregulation of SMAD2/3:SMAD4 transcriptional activity" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-4641257", "resource": "Reactome", "id": "R-MMU-4641257", "name": "Degradation of AXIN" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-5632684", "resource": "Reactome", "id": "R-MMU-5632684", "name": "Hedgehog 'on' state" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-5689880", "resource": "Reactome", "id": "R-MMU-5689880", "name": "Ub-specific processing proteases" }, { "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-8941858", "resource": "Reactome", "id": "R-MMU-8941858", "name": "Regulation of RUNX3 expression and activity" } ], "function": [ { "url": "https://www.uniprot.org/uniprot/A2A5Z6#function", "annotation": "E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A5Z6#function", "id": "A2A5Z6", "database": "UniProtKB" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_079757.2", "annotation": "Although conventional Smurf2 double knockout mice are early embryonic lethal, Cre-mediated recombination in mouse embryonic fibroblasts results in Smurf2 knockout cells that show it has roles in regulating many different signaling pathways and in genomic stability, cell polarity, tissue homeostasis and carcinogenesis Publication Status: Online-Only", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_079757.2", "id": "NP_079757.2", "database": "RefSeq" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_079757.2", "annotation": "Results identified Smurf2 as an essential regulator of Topo IIalpha, providing novel insights into its control and into the suggested tumor-suppressor functions of Smurf2.", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_079757.2", "id": "NP_079757.2", "database": "RefSeq" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_079757.2", "annotation": "Data suggest that Smad-specific E3 ubiquitin ligase 2 (SMURF2)-mediated SMAD3 protein (SMAD3) monoubiquitination interferes with the formation of a SMAD3-vitamin D receptor (VDR) complex. Publication Status: Online-Only", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_079757.2", "id": "NP_079757.2", "database": "RefSeq" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_079757.2", "annotation": "AIMP1 negatively regulates TGF-beta signaling via stabilization of Smurf2.", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_079757.2", "id": "NP_079757.2", "database": "RefSeq" } ] }, { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_079757.2", "annotation": "Tumor necrosis factor promotes Runx2 degradation through up-regulation of Smurf1 and Smurf2 in osteoblasts.", "evidence": [ { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_079757.2", "id": "NP_079757.2", "database": "RefSeq" } ] } ], "orthologs": [ { "uniprot_canonical_ac": "Q9HAU4-1", "protein_name": "E3 ubiquitin-protein ligase SMURF2; hSMURF2; 2.3.2.26; SMAD-specific E3 ubiquitin-protein ligase 2; SMAD ubiquitination regulatory factor 2; HECT-type E3 ubiquitin transferase SMURF2.", "sequence": { "length": 748, "sequence": "MSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE" }, "evidence": [ { "url": "http://www.informatics.jax.org/homology/41490", "id": "41490", "database": "MGI" } ], "organism": "Homo sapiens", "tax_id": 9606 } ], "refseq": { "url": "https://www.ncbi.nlm.nih.gov/protein/NP_079757.2", "ac": "NP_079757.2", "name": "E3 ubiquitin-protein ligase SMURF2 isoform 1; E3 ubiquitin-protein ligase SMURF2; SMAD ubiquitination regulatory factor 2; HECT-type E3 ubiquitin transferase SMURF2.", "summary": "" }, "go_annotation": { "categories": [ { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0061630", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A5Z6", "id": "A2A5Z6", "database": "UniProtKB" } ], "name": "ubiquitin protein ligase activity", "id": "GO:0061630" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0042802", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A5Z6", "id": "A2A5Z6", "database": "UniProtKB" } ], "name": "identical protein binding", "id": "GO:0042802" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005160", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A5Z6", "id": "A2A5Z6", "database": "UniProtKB" } ], "name": "transforming growth factor beta receptor binding", "id": "GO:0005160" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0046332", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A5Z6", "id": "A2A5Z6", "database": "UniProtKB" } ], "name": "SMAD binding", "id": "GO:0046332" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0004842", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A5Z6", "id": "A2A5Z6", "database": "UniProtKB" } ], "name": "ubiquitin-protein transferase activity", "id": "GO:0004842" } ], "total": 5, "name": "Molecular Function" }, { "go_terms": [ { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005737", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A5Z6", "id": "A2A5Z6", "database": "UniProtKB" } ], "name": "cytoplasm", "id": "GO:0005737" }, { "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005829", "evidence": [ { "url": "https://www.uniprot.org/uniprot/A2A5Z6", "id": "A2A5Z6", "database": "UniProtKB" } ], "name": "cytosol", "id": "GO:0005829" }, { "url": 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