{
    "uniprot_canonical_ac": "A0A0G2K730-1", 
    "sequence": {
        "header": "tr|A0A0G2K730-1|A0A0G2K730_RAT Isoform 1 of tRNA pseudouridine synthase  OS=Rattus norvegicus OX=10116 GN=Pusl1 PE=1 SV=1 CANONICAL=A0A0G2K730-1", 
        "length": 291, 
        "sequence": "MGSCGAVGSVRARYLVFFQYLGTDFNGVAAVRGNHRAVGVQNFLEEAAKRLNSVEPVRFTISSRTDAGVHALSNAAHLDIQRRPGRPPFSPEIVTKALNTHLKHPAIRVLKAFRVPNDFHARHAATSRTYLYRLATGCSGPNQLPVFEQNVCWSLQTEYLDIAAMQEAAQHLLGTHDFSAFQSAGSPVPHAVRTLRRVSVSPGPASLFVLPQESRRLQFWTLEFESQSFLYRQVRRMTAVLVAVGLGILAPTQVKVILESQDPLGKYQARVAPAHGLFLKSVLYDNFGPTS"
    }, 
    "uniprot_id": "A0A0G2K730_RAT", 
    "enzyme_annotation": [
        {
            "ec": "5.4.99.12", 
            "activity": "tRNA uridine(38-40) = tRNA pseudouridine(38-40)."
        }, 
        {
            "ec": "5.4.99.12", 
            "activity": ""
        }
    ], 
    "synonyms": {
        "protein": {
            "refseq": [
                "tRNA pseudouridine synthase-like 1 isoform X1"
            ], 
            "uniprotkb": [
                "tRNA pseudouridine synthase", 
                "5.4.99.12"
            ]
        }, 
        "gene": {
            "refseq": [
                "Pusl1"
            ], 
            "uniprotkb": [
                "Pusl1"
            ]
        }
    }, 
    "keywords": [], 
    "species": [
        {
            "evidence": [
                {
                    "url": "https://www.uniprot.org/uniprot/A0A0G2K730", 
                    "id": "A0A0G2K730", 
                    "database": "UniProtKB"
                }
            ], 
            "name": "Rattus norvegicus", 
            "taxid": 10116
        }
    ], 
    "recommendedname": {
        "full": "tRNA pseudouridine synthase; 5.4.99.12."
    }, 
    "publication": [], 
    "glycosylation": [], 
    "mutation": [], 
    "isoforms": [
        {
            "url": "https://www.uniprot.org/uniprot/A0A0G2K730#D3ZTC6-1", 
            "isoform_ac": "D3ZTC6-1", 
            "locus": {
                "end_pos": 173336874, 
                "start_pos": 173338769, 
                "chromosome": "5", 
                "evidence": [
                    {
                        "url": "https://www.ensembl.org/id/ENSRNOT00000067704", 
                        "id": "ENSRNOT00000067704", 
                        "database": "Ensembl Transcript"
                    }, 
                    {
                        "url": "https://www.ensembl.org/id/ENSRNOP00000059679", 
                        "id": "ENSRNOP00000059679", 
                        "database": "Ensembl Peptide"
                    }
                ]
            }, 
            "sequence": {
                "header": "tr|D3ZTC6-1|A0A0G2K730_RAT Computationally mapped isoform of tRNA pseudouridine synthase  OS=Rattus norvegicus OX=10116 GN=Pusl1 PE=1 SV=1 CANONICAL=A0A0G2K730-1", 
                "length": 127, 
                "sequence": "MQEAAQHLLGTHDFSAFQSAGSPVPHAVRTLRRVSVSPGPASLFVLPQESRRLQFWTLEFESQSFLYRQVRRMTAVLVAVGLGILAPTQVKVILESQDPLGKYQARVAPAHGLFLKSVLYDNFGWEN"
            }
        }, 
        {
            "url": "https://www.uniprot.org/uniprot/A0A0G2K730#A0A0G2K730-1", 
            "isoform_ac": "A0A0G2K730-1", 
            "locus": {
                "end_pos": 173337224, 
                "start_pos": 173339858, 
                "chromosome": "5", 
                "evidence": [
                    {
                        "url": "https://www.ensembl.org/id/ENSRNOT00000078453", 
                        "id": "ENSRNOT00000078453", 
                        "database": "Ensembl Transcript"
                    }, 
                    {
                        "url": "https://www.ensembl.org/id/ENSRNOP00000074040", 
                        "id": "ENSRNOP00000074040", 
                        "database": "Ensembl Peptide"
                    }
                ]
            }, 
            "sequence": {
                "header": "tr|A0A0G2K730-1|A0A0G2K730_RAT Isoform 1 of tRNA pseudouridine synthase  OS=Rattus norvegicus OX=10116 GN=Pusl1 PE=1 SV=1 CANONICAL=A0A0G2K730-1", 
                "length": 291, 
                "sequence": "MGSCGAVGSVRARYLVFFQYLGTDFNGVAAVRGNHRAVGVQNFLEEAAKRLNSVEPVRFTISSRTDAGVHALSNAAHLDIQRRPGRPPFSPEIVTKALNTHLKHPAIRVLKAFRVPNDFHARHAATSRTYLYRLATGCSGPNQLPVFEQNVCWSLQTEYLDIAAMQEAAQHLLGTHDFSAFQSAGSPVPHAVRTLRRVSVSPGPASLFVLPQESRRLQFWTLEFESQSFLYRQVRRMTAVLVAVGLGILAPTQVKVILESQDPLGKYQARVAPAHGLFLKSVLYDNFGPTS"
            }
        }
    ], 
    "pathway": [], 
    "function": [], 
    "orthologs": [
        {
            "uniprot_canonical_ac": "Q8N0Z8-1", 
            "protein_name": "tRNA pseudouridine synthase-like 1; 5.4.99.-; tRNA pseudouridylate synthase-like 1; tRNA-uridine isomerase-like 1.", 
            "sequence": {
                "length": 303, 
                "sequence": "MSSAPASGSVRARYLVYFQYVGTDFNGVAAVRGTQRAVGVQNYLEEAAERLNSVEPVRFTISSRTDAGVHALSNAAHLDVQRRSGRPPFPPEVLAEALNTHLRHPAIRVLRAFRVPSDFHARHAATSRTYLYRLATGCHRRDELPVFERNLCWTLPADCLDMVAMQEAAQHLLGTHDFSAFQSAGSPVPSPVRTLRRVSVSPGQASPLVTPEESRKLRFWNLEFESQSFLYRQVRRMTAVLVAVGLGALAPAQVKTILESQDPLGKHQTRVAPAHGLFLKSVLYGNLGAASCTLQGPQFGSHG"
            }, 
            "evidence": [
                {
                    "url": "http://www.informatics.jax.org/homology/13655", 
                    "id": "13655", 
                    "database": "MGI"
                }, 
                {
                    "url": "https://omabrowser.org/oma/group/A0A0G2K730/", 
                    "id": "A0A0G2K730", 
                    "database": "OMA"
                }
            ], 
            "organism": "Homo sapiens", 
            "tax_id": 9606
        }, 
        {
            "uniprot_canonical_ac": "A2ADA5-1", 
            "protein_name": "tRNA pseudouridine synthase-like 1; 5.4.99.-; tRNA pseudouridylate synthase-like 1; tRNA-uridine isomerase-like 1.", 
            "sequence": {
                "length": 291, 
                "sequence": "MGSCGAVGSVRARYLVFLQYLGTDFNGVAAVRGNPRAVGVLNFLEEAAKRLNSVDPVRFTISSRTDAGVHALSNAAHLDIQRRPGQSPFSPEVVAKALNTHLKHPAIRVLKAFRVPNDFHARHAATSRTYQYRLATGCSWPNQLPVFEQNVCWALQTEYLDMAAMQEAAQHLLGTHDFSAFQSAGSPVTNTVRTLRRVSVSPGPASPFVLPEGSRRLQFWTLEFESQSFLYRQVRRMTAVLVAVGLGILAPTQVKVILESQDPLGKYQARVAPARGLFLKSVLYDNFGPTS"
            }, 
            "evidence": [
                {
                    "url": "http://www.informatics.jax.org/homology/13655", 
                    "id": "13655", 
                    "database": "MGI"
                }, 
                {
                    "url": "https://omabrowser.org/oma/group/A0A0G2K730/", 
                    "id": "A0A0G2K730", 
                    "database": "OMA"
                }
            ], 
            "organism": "Mus musculus", 
            "tax_id": 10090
        }
    ], 
    "refseq": {
        "url": "https://www.ncbi.nlm.nih.gov/protein/XP_017449016.1", 
        "ac": "XP_017449016.1", 
        "name": "tRNA pseudouridine synthase-like 1 isoform X1.", 
        "summary": ""
    }, 
    "go_annotation": {
        "categories": [
            {
                "go_terms": [
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0009982", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A0A0G2K730", 
                                "id": "A0A0G2K730", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "pseudouridine synthase activity", 
                        "id": "GO:0009982"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0003723", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A0A0G2K730", 
                                "id": "A0A0G2K730", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "RNA binding", 
                        "id": "GO:0003723"
                    }, 
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0106029", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A0A0G2K730", 
                                "id": "A0A0G2K730", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "tRNA pseudouridine synthase activity", 
                        "id": "GO:0106029"
                    }
                ], 
                "total": 3, 
                "name": "Molecular Function"
            }, 
            {
                "go_terms": [
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0043231", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A0A0G2K730", 
                                "id": "A0A0G2K730", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "intracellular membrane-bounded organelle", 
                        "id": "GO:0043231"
                    }
                ], 
                "total": 1, 
                "name": "Cellular Component"
            }, 
            {
                "go_terms": [
                    {
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0031119", 
                        "evidence": [
                            {
                                "url": "https://www.uniprot.org/uniprot/A0A0G2K730", 
                                "id": "A0A0G2K730", 
                                "database": "UniProtKB"
                            }
                        ], 
                        "name": "tRNA pseudouridine synthesis", 
                        "id": "GO:0031119"
                    }
                ], 
                "total": 1, 
                "name": "Biological Process"
            }
        ]
    }, 
    "site_annotation": [], 
    "crossref": [
        {
            "url": "https://www.ncbi.nlm.nih.gov/protein/XP_017449016.1", 
            "id": "XP_017449016.1", 
            "database": "RefSeq"
        }, 
        {
            "url": "https://bgee.org/?page=gene&gene_id=ENSRNOG00000022354", 
            "id": "ENSRNOG00000022354", 
            "database": "Bgee"
        }, 
        {
            "url": "https://omabrowser.org/oma/group/FLYGMVR/", 
            "id": "FLYGMVR", 
            "database": "OMA"
        }, 
        {
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR001406", 
            "id": "IPR001406", 
            "database": "InterPro"
        }, 
        {
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR020095", 
            "id": "IPR020095", 
            "database": "InterPro"
        }, 
        {
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR020097", 
            "id": "IPR020097", 
            "database": "InterPro"
        }, 
        {
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR020103", 
            "id": "IPR020103", 
            "database": "InterPro"
        }, 
        {
            "url": "http://pfam.xfam.org/family/PF01416", 
            "id": "PF01416", 
            "database": "Pfam"
        }, 
        {
            "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR11142", 
            "id": "PTHR11142", 
            "database": "PANTHER"
        }, 
        {
            "url": "https://www.ncbi.nlm.nih.gov/gene/362681", 
            "id": "362681", 
            "database": "GeneID"
        }, 
        {
            "url": "http://rgd.mcw.edu/tools/genes/genes_view.cgi?id=1559560", 
            "id": "1559560", 
            "database": "RGD"
        }
    ], 
    "expression_disease": [], 
    "uniprot_ac": "A0A0G2K730", 
    "disease": [], 
    "mass": {
        "monoisotopic_mass": 32089.0, 
        "chemical_mass": 32089.0
    }, 
    "expression_tissue": [], 
    "gene": [
        {
            "url": "https://www.uniprot.org/uniprot/A0A0G2K730", 
            "name": "Pusl1.", 
            "locus": {
                "end_pos": 173336034, 
                "start_pos": 173340026, 
                "chromosome": "5", 
                "evidence": [
                    {
                        "url": "https://www.ensembl.org/id/ENSRNOG00000022354", 
                        "id": "ENSRNOG00000022354", 
                        "database": "Ensembl Gene"
                    }
                ]
            }
        }
    ]
}