{
    "bcoid": "GLY_000143",
    "title": "Human Protein Glycosylation (Literature)",
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        "species": "Homo sapiens",
        "molecule": "Proteoform",
        "tags": "UniCarbKB publications",
        "status": "Reviewed",
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        "scope": "external"
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    "readme": "Name\n   - Human Protein Glycosylation (Literature)\n\nVersion\n   - 1.4.5\n\nCreated on\n  - 2019-06-14T14:54:26.195339\n\nModified on\n   - 2019-09-12T16:37:13.572422\n\nCreated by\n   - Rahi Navelkar [rsn13@gwu.edu], Jeet Vora [jeetvora@gwu.edu], Robel Kahsay [rykahsay@gwu.edu], Xavier Holmes [xavierh@email.gwu.edu]\n\nAuthors\n   - \n\nChecksum\n   - \n\nUsability Domain\n   - The dataset provides information on N-glycosylation sites on Human proteins. The data has been processed from the supplementary material from 2 publications (1. \"Deeb, S. J., Cox, J., Schmidt-Supprian, M., & Mann, M. (2013). N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics, 13(1), 240-251. doi:10.1074/mcp.m113.033977\" 2. \"Boersema, P. J., Geiger, T., Wi?niewski, J. R., & Mann, M. (2012). Quantification of theN-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics, 12(1), 158-171. doi:10.1074/mcp.m112.023614\").  The listed protein (UniProtKB) accessions are part of the GlyGen UniProtKB canonical list (https://data.glygen.org/GLYDS000001). \n\nDescription Domain\n   Keywords\n   - proteins\n   - canonical\n   - glycosylation\n   - literature\n   - publications\n\n   Pipeline Steps\n   - Step-1: The supplementary tables from two publications were downloaded and columns \"protein\" and \"site\" were extracted, and additional column evidence where values=\"the respective PMIDs\" was added to produce the input files. A python script was developed process input files 1. to map the UniProtKB accessions to UniProtKB canonical accessions 2. Check position+amino acid with the canonical fasta sequences (https://data.glygen.org/GLYDS000002)to generate the output files.\n\nExecution Domain\n   Scripts   - https://github.com/GW-HIVE/glygen-backend-integration/blob/master/pipeline/integrator/make-proteoform-dataset.py\n\n\n   Software Prerequisites\n      Name: Python\n      Version: 2.7.5\n      URI: https://www.python.org/download/releases/2.7.5/\n\nI/O Domain\n   Input Subdomain\n      Name: glycosylation_sites_24190977_s5.csv\n      URI: https://data.glygen.org/ln2data/releases/data/v-1.4.5/source/glycosylation_sites_24190977_s5.csv\n      Name: glycosylation_sites_23090970_s5.csv\n      URI: https://data.glygen.org/ln2data/releases/data/v-1.4.5/source/glycosylation_sites_23090970_s5.csv\n      Name: glycosylation_sites_23090970_s2.csv\n      URI: https://data.glygen.org/ln2data/releases/data/v-1.4.5/source/glycosylation_sites_23090970_s2.csv\n   Output Subdomain\n      Name: human_proteoform_glycosylation_sites_literature.csv\n      URI: http://data.glygen.org/ln2data/releases/data/v-1.4.5/reviewed/human_proteoform_glycosylation_sites_literature.csv\n      Name: human_proteoform_glycosylation_sites_literature.stat.csv\n      URI: http://data.glygen.org/ln2data/releases/data/v-1.4.5/reviewed/human_proteoform_glycosylation_sites_literature.stat.csv\n\nFields\n      Name: \"uniprotkb_canonical_ac\"\n     Description: \"Accession assigned to the protein isoform chosen to be the canonical sequence to which all positional annotation refers in UniProtKB database\"\n      Unique Values: \"1340\"\n      Name: \"glycosylation_site_uniprotkb\"\n     Description: \"Site on the protein sequence of the UniProtKB entry where glycosylation is observed\"\n      Unique Values: \"1045\"\n      Name: \"glycosylation_type\"\n     Description: \"Type of glycosylation (N/O/C/S linked glycosylation)\"\n      Unique Values: \"1\"\n      Name: \"amino_acid\"\n     Description: \"Three letter abbreviation code of the amino acid\"\n      Unique Values: \"1\"\n      Name: \"evidence\"\n     Description: \"NCBI PubMed Id (PMID) as evidence for the entry/annotation\"\n      Unique Values: \"2\"\n      Name: \"saccharide\"\n     Description: \"Sugar\"\n      Unique Values: \"1\"",
    "downloadurl": "/ln2data/releases/data/v-1.4.5/reviewed/human_proteoform_glycosylation_sites_literature.csv"
}
