{ "uniprot_canonical_ac": "A2AS55-1", "uniprot_ac": "A2AS55", "uniprot_id": "ANR16_MOUSE", "mass": { "chemical_mass": 39776.0, "monoisotopic_mass": 39776.0 }, "refseq": { "ac": "NP_796242", "name": "ankyrin repeat domain-containing protein 16 isoform 1", "summary": "", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_796242" }, "gene_names": [ { "name": "Ankrd16", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/A2AS55", "id": "A2AS55" }, { "name": "2810455F06Rik; AI646698; D430029B21Rik", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_796242", "id": "NP_796242" }, { "name": "Ankrd16", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_796242", "id": "NP_796242" } ], "protein_names": [ { "name": "Ankyrin repeat domain-containing protein 16", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/A2AS55#names_and_taxonomy", "id": "A2AS55" }, { "name": "Ankyrin repeat domain-containing protein 16", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/A2AS55#names_and_taxonomy", "id": "A2AS55" }, { "name": "ankyrin repeat domain-containing protein 16 isoform 1", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_796242", "id": "NP_796242" }, { "name": "ankyrin repeat domain-containing protein 16", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_796242", "id": "NP_796242" } ], "function": [ { "annotation": "Required to prevent the misactivation of serine (Ser) with tRNA(Ala) by promoting the hydrolysis of Ser-mischarged tRNA(Ala), thereby playing a role in translational fidelity (PubMed:29769718). Binds directly to the catalytic domain of AARS/AlaRS and captures Ser that is misactivated by AARS/AlaRS, preventing the charging of Ser adenylates to tRNA(Ala) and precluding Ser misincorporation in nascent peptides (PubMed:29769718).", "evidence": [ { "database": "UniProtKB", "id": "A2AS55", "url": "https://www.uniprot.org/uniprot/A2AS55#function" } ] }, { "annotation": "Results identify an amino-acid-accepting co-regulator of tRNA synthetase editing, ANKRD16, as a new layer of the machinery that is essential to the prevention of severe pathologies that arise from defects in editing Erratum:[Nature. 2018 Aug;560(7720):E35. PMID: 29925958]", "evidence": [ { "database": "RefSeq", "id": "NP_796242", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_796242" } ] } ], "disease": [], "snv": [], "mutagenesis": [ { "start_pos": 102, "end_pos": 102, "sequence_org": "K", "sequence_mut": "R", "comment": "In ANKRD16(3XR); abolished ability to enhance hydrolysis of Ser-mischarged tRNA(Ala); when associated with R-135 and R-165.", "evidence": [ { "database": "UniProtKB", "id": "A2AS55", "url": "https://www.uniprot.org/uniprot/A2AS55" }, { "database": "PubMed", "id": "29769718", "url": "https://www.ncbi.nlm.nih.gov/pubmed/29769718" } ] }, { "start_pos": 135, "end_pos": 135, "sequence_org": "K", "sequence_mut": "R", "comment": "In ANKRD16(3XR); abolished ability to enhance hydrolysis of Ser-mischarged tRNA(Ala); when associated with R-102 and R-165.", "evidence": [ { "database": "UniProtKB", "id": "A2AS55", "url": "https://www.uniprot.org/uniprot/A2AS55" }, { "database": "PubMed", "id": "29769718", "url": "https://www.ncbi.nlm.nih.gov/pubmed/29769718" } ] }, { "start_pos": 165, "end_pos": 165, "sequence_org": "K", "sequence_mut": "R", "comment": "In ANKRD16(3XR); abolished ability to enhance hydrolysis of Ser-mischarged tRNA(Ala); when associated with R-102 and R-135.", "evidence": [ { "database": "UniProtKB", "id": "A2AS55", "url": "https://www.uniprot.org/uniprot/A2AS55" }, { "database": "PubMed", "id": "29769718", "url": "https://www.ncbi.nlm.nih.gov/pubmed/29769718" } ] } ], "expression_tissue": [], "expression_disease": [], "species": [ { "name": "Mus musculus", "taxid": 10090, "evidence": [ { "database": "UniProtKB", "id": "A2AS55", "url": "https://www.uniprot.org/uniprot/A2AS55" } ], "common_name": "Mouse" } ], "gene": [ { "name": "Ankrd16", "url": "https://www.uniprot.org/uniprot/A2AS55", "locus": { "chromosome": "2", "start_pos": 11777876, "end_pos": 11790329, "strand": "+", "evidence": [ { "database": "Ensembl Gene", "id": "ENSMUSG00000047909", "url": "https://www.ensembl.org/id/ENSMUSG00000047909" } ] } } ], "crossref": [ { "id": "ENSMUSG00000047909", "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000047909", "database": "Bgee" }, { "id": "320816", "url": "https://www.ncbi.nlm.nih.gov/gene/320816", "database": "GeneID" }, { "id": "IPR002110", "url": "https://www.ebi.ac.uk/interpro/entry/IPR002110", "database": "InterPro" }, { "id": "IPR020683", "url": "https://www.ebi.ac.uk/interpro/entry/IPR020683", "database": "InterPro" }, { "id": "IPR036770", "url": "https://www.ebi.ac.uk/interpro/entry/IPR036770", "database": "InterPro" }, { "id": "mmu:320816", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:320816", "database": "KEGG Pathway" }, { "id": "2444796", "url": "http://www.informatics.jax.org/marker/MGI:2444796", "database": "MGI" }, { "id": "CGLTPFM", "url": "https://omabrowser.org/oma/group/CGLTPFM/", "database": "OMA" }, { "id": "1073161at2759", "url": "http://www.orthodb.org/?query=1073161at2759", "database": "OrthoDB" }, { "id": "PF00023", "url": "http://pfam.xfam.org/family/PF00023", "database": "Pfam" }, { "id": "PF12796", "url": "http://pfam.xfam.org/family/PF12796", "database": "Pfam" }, { "id": "PR_A2AS55", "url": "http://purl.obolibrary.org/obo/PR_PR_A2AS55", "database": "PRO" }, { "id": "NP_796242", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_796242", "database": "RefSeq" }, { "id": "A0A0A6YWT3", "url": "https://www.uniprot.org/uniprot/A0A0A6YWT3", "database": "UniProtKB" }, { "id": "A0A0A6YWT3-1", "url": "https://www.uniprot.org/uniprot/A0A0A6YWT3-1", "database": "UniProtKB" }, { "id": "A2AS55", "url": "https://www.uniprot.org/uniprot/A2AS55", "database": "UniProtKB" }, { "id": "A2AS55-1", "url": "https://www.uniprot.org/uniprot/A2AS55-1", "database": "UniProtKB" }, { "id": "A2AS55-2", "url": "https://www.uniprot.org/uniprot/A2AS55-2", "database": "UniProtKB" }, { "id": "D6RGL4", "url": "https://www.uniprot.org/uniprot/D6RGL4", "database": "UniProtKB" }, { "id": "D6RGL4-1", "url": "https://www.uniprot.org/uniprot/D6RGL4-1", "database": "UniProtKB" }, { "id": "F6SD06", "url": "https://www.uniprot.org/uniprot/F6SD06", "database": "UniProtKB" }, { "id": "F6SD06-1", "url": "https://www.uniprot.org/uniprot/F6SD06-1", "database": "UniProtKB" }, { "id": "F6T2W3", "url": "https://www.uniprot.org/uniprot/F6T2W3", "database": "UniProtKB" }, { "id": "F6T2W3-1", "url": "https://www.uniprot.org/uniprot/F6T2W3-1", "database": "UniProtKB" } ], "sequence": { "sequence": "MALPGDPRRLCRLVQEGRLRDLQEELAVARGCRGPAGDTLLHCAARHGRQDILAYLVEAWSMDIEATNRDYKRPLHEAASMGHRDCVRYLLGRGAVVDSLKKADWTPLMMACTRKNLDVIQDLVEHGANPLLKNKDGWNSFHIASREGHPVILRYLLTVCPDAWKTESNIRRTPLHTAAMHGCLEAVQVLLERCHYEPDCRDNCGVTPFMDAIQCGHVSIAKLLLEQHKACSSAADSMGAQALHRAAVTGQDEAIRFLVCGLGIDVDVRAKSSQLTALHYAAKEGQTNTVQTLLSLGADINSTDERNRSVLHLACAGQHVACTRLLLQSGLKDSEDLTGTLAQQLTRSVDILQDFDHDVKS", "length": 361, "header": "sp|A2AS55-1|ANR16_MOUSE Isoform 1 of Ankyrin repeat domain-containing protein 16 OS=Mus musculus OX=10090 GN=Ankrd16 PE=1 SV=1 CANONICAL=A2AS55-1" }, "isoforms": [ { "isoform_ac": "A0A0A6YWT3-1", "url": "https://www.uniprot.org/uniprot/A2AS55#A0A0A6YWT3-1", "sequence": { "sequence": "MALPGDPRRLCRLVQEGRLRDLQEELAVARGCRGPAGDTLLHCAARHGRQDILAYLVEAWSMDIEATNRDYKRPLHEAASMGHRDCVRYL", "length": 90, "header": "tr|A0A0A6YWT3-1|A0A0A6YWT3_MOUSE Computationally mapped isoform of Ankyrin repeat domain-containing protein 16 OS=Mus musculus OX=10090 GN=Ankrd16 PE=1 SV=1 CANONICAL=A2AS55-1" }, "locus": { "chromosome": "2", "start_pos": 11783669, "end_pos": 11783400, "strand": "-", "evidence": [ { "database": "Ensembl Transcript", "id": "ENSMUST00000130186", "url": "https://www.ensembl.org/id/ENSMUST00000130186" }, { "database": "Ensembl Peptide", "id": "ENSMUSP00000141685", "url": "https://www.ensembl.org/id/ENSMUSP00000141685" } ] } }, { "isoform_ac": "A2AS55-1", "url": "https://www.uniprot.org/uniprot/A2AS55#A2AS55-1", "sequence": { "sequence": "MALPGDPRRLCRLVQEGRLRDLQEELAVARGCRGPAGDTLLHCAARHGRQDILAYLVEAWSMDIEATNRDYKRPLHEAASMGHRDCVRYLLGRGAVVDSLKKADWTPLMMACTRKNLDVIQDLVEHGANPLLKNKDGWNSFHIASREGHPVILRYLLTVCPDAWKTESNIRRTPLHTAAMHGCLEAVQVLLERCHYEPDCRDNCGVTPFMDAIQCGHVSIAKLLLEQHKACSSAADSMGAQALHRAAVTGQDEAIRFLVCGLGIDVDVRAKSSQLTALHYAAKEGQTNTVQTLLSLGADINSTDERNRSVLHLACAGQHVACTRLLLQSGLKDSEDLTGTLAQQLTRSVDILQDFDHDVKS", "length": 361, "header": "sp|A2AS55-1|ANR16_MOUSE Isoform 1 of Ankyrin repeat domain-containing protein 16 OS=Mus musculus OX=10090 GN=Ankrd16 PE=1 SV=1 CANONICAL=A2AS55-1" }, "locus": { "chromosome": "2", "start_pos": 11794699, "end_pos": 11783400, "strand": "-", "evidence": [ { "database": "Ensembl Transcript", "id": "ENSMUST00000056108", "url": "https://www.ensembl.org/id/ENSMUST00000056108" }, { "database": "Ensembl Peptide", "id": "ENSMUSP00000052056", "url": "https://www.ensembl.org/id/ENSMUSP00000052056" } ] } }, { "isoform_ac": "A2AS55-2", "url": "https://www.uniprot.org/uniprot/A2AS55#A2AS55-2", "sequence": { "sequence": "MALPGDPRRLCRLVQEGRLRDLQEELAVARGCRGPAGDTLLHCAARHGRQDILAYLVEAWSMDIEATNRDYKRPLHEAASMGHRDCVRYLLGRGAVVDSLKKADWTPLMMACTRKNLDVIQDLVEHGANPLLKNKDGWNSFHIASREGHPVILRYLLTVCPDAWKTESNIRRTPLHTAGTPCKLWSL", "length": 187, "header": "sp|A2AS55-2|ANR16_MOUSE Isoform 2 of Ankyrin repeat domain-containing protein 16 OS=Mus musculus OX=10090 GN=Ankrd16 PE=1 SV=1 CANONICAL=A2AS55-1" }, "locus": {} }, { "isoform_ac": "D6RGL4-1", "url": "https://www.uniprot.org/uniprot/A2AS55#D6RGL4-1", "sequence": { "sequence": "MALPGDPRRLCRLVQEGRLRDLQEELAVARGCRGPAGDTLLHCAARHGRQDILAYLVEAWSMDIEATNRDYKRPLHEAASMGHRDCVRYLLGRGAVVDSLKKADWTPLMMACTRKNLDVIQDLVEHGANPLLKNKDGWNSFHIASREGHPVILRNARLFGSSPGAS", "length": 166, "header": "tr|D6RGL4-1|D6RGL4_MOUSE Computationally mapped isoform of Ankyrin repeat domain-containing protein 16 OS=Mus musculus OX=10090 GN=Ankrd16 PE=1 SV=1 CANONICAL=A2AS55-1" }, "locus": { "chromosome": "2", "start_pos": 11786324, "end_pos": 11783400, "strand": "-", "evidence": [ { "database": "Ensembl Transcript", "id": "ENSMUST00000156067", "url": "https://www.ensembl.org/id/ENSMUST00000156067" }, { "database": "Ensembl Peptide", "id": "ENSMUSP00000115525", "url": "https://www.ensembl.org/id/ENSMUSP00000115525" } ] } }, { "isoform_ac": "F6SD06-1", "url": "https://www.uniprot.org/uniprot/A2AS55#F6SD06-1", "sequence": { "sequence": "LHTAAMHGCLEAVQVLLERCHYEPDCRDNCGVTPFMDAIQCGHVSIAKLLLEQHKV", "length": 56, "header": "tr|F6SD06-1|F6SD06_MOUSE Computationally mapped isoform of Ankyrin repeat domain-containing protein 16 OS=Mus musculus OX=10090 GN=Ankrd16 PE=4 SV=2 CANONICAL=A2AS55-1" }, "locus": { "chromosome": "2", "start_pos": 11788563, "end_pos": 11784696, "strand": "-", "evidence": [ { "database": "Ensembl Transcript", "id": "ENSMUST00000133664", "url": "https://www.ensembl.org/id/ENSMUST00000133664" }, { "database": "Ensembl Peptide", "id": "ENSMUSP00000115226", "url": "https://www.ensembl.org/id/ENSMUSP00000115226" } ] } }, { "isoform_ac": "F6T2W3-1", "url": "https://www.uniprot.org/uniprot/A2AS55#F6T2W3-1", "sequence": { "sequence": "XCSSAADSMGAQALHRAAVTGQDEAIRFLVCGLGIDVDVRAKSSQLTALHYAAKLQRICGVTPFMDTLQFSHVCIA", "length": 76, "header": "tr|F6T2W3-1|F6T2W3_MOUSE Computationally mapped isoform of Ankyrin repeat domain-containing protein 16 OS=Mus musculus OX=10090 GN=Ankrd16 PE=1 SV=2 CANONICAL=A2AS55-1" }, "locus": { "chromosome": "2", "start_pos": 11790048, "end_pos": 11789104, "strand": "-", "evidence": [ { "database": "Ensembl Transcript", "id": "ENSMUST00000128774", "url": "https://www.ensembl.org/id/ENSMUST00000128774" }, { "database": "Ensembl Peptide", "id": "ENSMUSP00000121218", "url": "https://www.ensembl.org/id/ENSMUSP00000121218" } ] } } ], "glycosylation": [], "phosphorylation": [], "glycation": [], "keywords": [], "publication": [ { "title": "ANKRD16 prevents neuron loss caused by an editing-defective tRNA synthetase.", "journal": "Nature", "date": "2018", "authors": "Moresco J.J., Sun L., Yates J.R. III, Fu H., Liu Q., Schimmel P., Roy B., Lee J.W., Terrey M., Ackerman S.L., Vo M.N., Fredrick K., Weber T.G.", "evidence": [ { "database": "UniProtKB", "id": "A2AS55", "url": "https://www.uniprot.org/uniprot/A2AS55" }, { "database": "RefSeq", "id": "NP_796242", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_796242" } ], "reference": [ { "type": "PubMed", "id": "29769718", "url": "https://www.ncbi.nlm.nih.gov/pubmed/29769718" } ] } ], "pathway": [ { "id": "mmu:320816", "name": "", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:320816", "resource": "KEGG Pathway" } ], "orthologs": [ { "uniprot_canonical_ac": "Q499M5-1", "protein_name": "Ankyrin repeat domain-containing protein 16", "gene_name": "Ankrd16", "tax_id": 10116, "organism": "Rattus norvegicus", "sequence": { "sequence": "MAPPGDPRRLCRLVQEGQLRALREELEVAGGCWDPEMFRGSQGPAGDTLLHFASRHGRQDILAYLVEAWSMDIEAANRDYKRPLHEAASMGHRDCVRYLLGRGAVVDSLKKADWTPLMMACTRKNLEVIQDLVEHGANPLLKNKDGWNSFHIASREGDPVILRYLLTVCPDVWKTESKIRRTPLHTAAMHGCFEAVQELLERCHYEPDCRDNCGVTPFMDAIQCGHVSIAKLLLENHEACSSATDSLGAQALHRAAVTGQDEAIRFLVCGLGIDVDVRAKSSQLTALLYAAKEGQTSTVQTLLSLGADINSTDERNRSALHLACAGQHAACARFLRQSGLKDSADLTGALAQQLTRGVAILQDFDPGVKS", "length": 370 }, "evidence": [ { "id": "19509", "url": "http://www.informatics.jax.org/homology/19509", "database": "MGI" }, { "id": "A2AS55", "url": "https://omabrowser.org/oma/group/A2AS55/", "database": "OMA" } ], "common_name": "Rat" }, { "uniprot_canonical_ac": "Q6P6B7-1", "protein_name": "Ankyrin repeat domain-containing protein 16", "gene_name": "ANKRD16", "tax_id": 9606, "organism": "Homo sapiens", "sequence": { "sequence": "MAQPGDPRRLCRLVQEGRLRALKEELQAAGGCPGPAGDTLLHCAARHGHRDVLAYLAEAWGMDIEATNRDYKRPLHEAASMGHRDCVRYLLGRGAAVDCLKKADWTPLMMACTRKNLGVIQELVEHGANPLLKNKDGWNSFHIASREGDPLILQYLLTVCPGAWKTESKIRRTPLHTAAMHGHLEAVKVLLKRCQYEPDYRDNCGVTALMDAIQCGHIDVARLLLDEHGACLSAEDSLGAQALHRAAVTGQDEAIRFLVSELGVDVDVRATSTHLTALHYAAKEGHTSTIQTLLSLGADINSKDEKNRSALHLACAGQHLACAKFLLQSGLKDSEDITGTLAQQLPRRADVLQGSGHSAMT", "length": 361 }, "evidence": [ { "id": "19509", "url": "http://www.informatics.jax.org/homology/19509", "database": "MGI" } ], "common_name": "Human" } ], "go_annotation": { "categories": [ { "name": "Cellular Component", "total": 2, "go_terms": [ { "name": "nucleus", "id": "GO:0005634", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005634", "evidence": [ { "database": "UniProtKB", "id": "A2AS55", "url": "https://www.uniprot.org/uniprot/A2AS55" } ], "pmid": "29769718" }, { "name": "cytoplasm", "id": "GO:0005737", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005737", "evidence": [ { "database": "UniProtKB", "id": "A2AS55", "url": "https://www.uniprot.org/uniprot/A2AS55" } ], "pmid": "29769718" } ] }, { "name": "Biological Process", "total": 1, "go_terms": [ { "name": "tRNA modification", "id": "GO:0006400", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0006400", "evidence": [ { "database": "UniProtKB", "id": "A2AS55", "url": "https://www.uniprot.org/uniprot/A2AS55" } ], "pmid": "29769718" } ] } ] }, "pro_annotation": [], "site_annotation": [ { "start_pos": 301, "end_pos": 303, "annotation": "n_glycosylation_sequon" }, { "start_pos": 307, "end_pos": 309, "annotation": "n_glycosylation_sequon" } ], "ptm_annotation": [], "interactions": [], "reactions": [], "reaction_participants": [], "reaction_enzymes": [], "synthesized_glycans": [], "cluster_types": [ { "name": "isoformset.uniprotkb", "label": "Isoforms" }, { "name": "homologset.mgi", "label": "MGI Homologs" }, { "name": "homologset.oma", "label": "OMA Homologs" } ] }