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    {
        "title": "Rlp24 activates the AAA-ATPase Drg1 to initiate cytoplasmic pre-60S maturation.",
        "journal": "The Journal of cell biology",
        "date": "2012-11-26",
        "authors": "Kappel Lisa, Loibl Mathias, Zisser Gertrude, Klein Isabella, Fruhmann Gernot, Gruber Christof, Unterweger Stefan, Rechberger Gerald, Pertschy Brigitte, Bergler Helmut",
        "reference": {
            "type": "PubMed",
            "id": "23185031",
            "url": "https://pubmed.ncbi.nlm.nih.gov/23185031"
        },
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            {
                "uniprot_canonical_ac": "Q02630-1",
                "protein_name": "Nucleoporin NUP116/NSP116",
                "gene_name": "NUP116",
                "tax_name": "Baker's yeast"
            },
            {
                "uniprot_canonical_ac": "Q07915-1",
                "protein_name": "Ribosome biogenesis protein RLP24",
                "gene_name": "RLP24",
                "tax_name": "Baker's yeast"
            },
            {
                "uniprot_canonical_ac": "P32794-1",
                "protein_name": "ATPase family gene 2 protein",
                "gene_name": "AFG2",
                "tax_name": "Baker's yeast"
            }
        ],
        "species": [
            {
                "name": "Saccharomyces cerevisiae S288C",
                "taxid": 559292,
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q02630",
                        "url": "https://www.uniprot.org/uniprotkb/Q02630"
                    }
                ],
                "common_name": "Baker's yeast",
                "glygen_name": "Yeast",
                "reference_species": "Saccharomyces cerevisiae S288C [559292]"
            }
        ],
        "mutagenesis": [
            {
                "start_pos": 110,
                "end_pos": 166,
                "sequence_org": "VVPNSTAGTSIKPFTTFEEKDPTTGVINVFQSITCMPEYRNFSFEELRFQDYQAGRK",
                "sequence_mut": "",
                "comment": "Prevents the release of AFG2, RLP24 and NOG1 from pre-60S ribosomal particles. Enhances growth defect in an AFG2 (drg1-18) mutant background.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q02630",
                        "url": "https://www.uniprot.org/uniprotkb/Q02630"
                    },
                    {
                        "database": "PubMed",
                        "id": "23185031",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/23185031"
                    }
                ],
                "uniprot_canonical_ac": "Q02630-1"
            },
            {
                "start_pos": 173,
                "end_pos": 1113,
                "sequence_org": "GTGTTFNNPQGTTNTGFGIMGNNNSTTSATTGGLFGQKPATGMFGTGTGSGGGFGSGATNSTGLFGSSTNLSGNSAFGANKPATSGGLFGNTTNNPTNGTNNTGLFGQQNSNTNGGLFGQQQNSFGANNVSNGGAFGQVNRGAFPQQQTQQGSGGIFGQSNANANGGAFGQQQGTGALFGAKPASGGLFGQSAGSKAFGMNTNPTGTTGGLFGQTNQQQSGGGLFGQQQNSNAGGLFGQNNQSQNQSGLFGQQNSSNAFGQPQQQGGLFGSKPAGGLFGQQQGASTFASGNAQNNSIFGQNNQQQQSTGGLFGQQNNQSQSQPGGLFGQTNQNNNQPFGQNGLQQPQQNNSLFGAKPTGFGNTSLFSNSTTNQSNGISGNNLQQQSGGLFQNKQQPASGGLFGSKPSNTVGGGLFGNNQVANQNNPASTSGGLFGSKPATGSLFGGTNSTAPNASSGGIFGSNNASNTAATTNSTGLFGNKPVGAGASTSAGGLFGNNNNSSLNNSNGSTGLFGSNNTSQSTNAGGLFQNNTSTNTSGGGLFSQPSQSMAQSQNALQQQQQQQRLQIQNNNPYGTNELFSKATVTNTVSYPIQPSATKIKADERKKASLTNAYKMIPKTLFTAKLKTNNSVMDKAQIKVDPKLSISIDKKNNQIAISNQQEENLDESILKASELLFNPDKRSFKNLINNRKMLIASEEKNNGSQNNDMNFKSKSEEQETILGKPKMDEKETANGGERMVLSSKNDGEDSATKHHSRNMDEENKENVADLQKQEYSEDDKKAVFADVAEKDASFINENYYISPSLDTLSSYSLLQLRKVPHLVVGHKSYGKIEFLEPVDLAGIPLTSLGGVIITFEPKTCIIYANLPNRPKRGEGINVRARITCFNCYPVDKSTRKPIKDPNHQLVKRHIERLKKNPNSKFESYDADSGTYVFIVNHAAEQT",
                "sequence_mut": "",
                "comment": "No effect on the interaction with AFG2.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q02630",
                        "url": "https://www.uniprot.org/uniprotkb/Q02630"
                    },
                    {
                        "database": "PubMed",
                        "id": "23185031",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/23185031"
                    }
                ],
                "uniprot_canonical_ac": "Q02630-1"
            },
            {
                "start_pos": 92,
                "end_pos": 1113,
                "sequence_org": "SIFGGMGNNTALSAGSASVVPNSTAGTSIKPFTTFEEKDPTTGVINVFQSITCMPEYRNFSFEELRFQDYQAGRKFGTSQNGTGTTFNNPQGTTNTGFGIMGNNNSTTSATTGGLFGQKPATGMFGTGTGSGGGFGSGATNSTGLFGSSTNLSGNSAFGANKPATSGGLFGNTTNNPTNGTNNTGLFGQQNSNTNGGLFGQQQNSFGANNVSNGGAFGQVNRGAFPQQQTQQGSGGIFGQSNANANGGAFGQQQGTGALFGAKPASGGLFGQSAGSKAFGMNTNPTGTTGGLFGQTNQQQSGGGLFGQQQNSNAGGLFGQNNQSQNQSGLFGQQNSSNAFGQPQQQGGLFGSKPAGGLFGQQQGASTFASGNAQNNSIFGQNNQQQQSTGGLFGQQNNQSQSQPGGLFGQTNQNNNQPFGQNGLQQPQQNNSLFGAKPTGFGNTSLFSNSTTNQSNGISGNNLQQQSGGLFQNKQQPASGGLFGSKPSNTVGGGLFGNNQVANQNNPASTSGGLFGSKPATGSLFGGTNSTAPNASSGGIFGSNNASNTAATTNSTGLFGNKPVGAGASTSAGGLFGNNNNSSLNNSNGSTGLFGSNNTSQSTNAGGLFQNNTSTNTSGGGLFSQPSQSMAQSQNALQQQQQQQRLQIQNNNPYGTNELFSKATVTNTVSYPIQPSATKIKADERKKASLTNAYKMIPKTLFTAKLKTNNSVMDKAQIKVDPKLSISIDKKNNQIAISNQQEENLDESILKASELLFNPDKRSFKNLINNRKMLIASEEKNNGSQNNDMNFKSKSEEQETILGKPKMDEKETANGGERMVLSSKNDGEDSATKHHSRNMDEENKENVADLQKQEYSEDDKKAVFADVAEKDASFINENYYISPSLDTLSSYSLLQLRKVPHLVVGHKSYGKIEFLEPVDLAGIPLTSLGGVIITFEPKTCIIYANLPNRPKRGEGINVRARITCFNCYPVDKSTRKPIKDPNHQLVKRHIERLKKNPNSKFESYDADSGTYVFIVNHAAEQT",
                "sequence_mut": "",
                "comment": "Loss of interaction with AFG2.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q02630",
                        "url": "https://www.uniprot.org/uniprotkb/Q02630"
                    },
                    {
                        "database": "PubMed",
                        "id": "23185031",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/23185031"
                    }
                ],
                "uniprot_canonical_ac": "Q02630-1"
            },
            {
                "start_pos": 147,
                "end_pos": 199,
                "sequence_org": "KEQERAESVSEQEESEEEEEDMEIDSDEEEEEQLEKQKILLKNRRRNTKKIAF",
                "sequence_mut": "",
                "comment": "Reduces interaction with AFG2. Loss of AFG2 activation.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q07915",
                        "url": "https://www.uniprot.org/uniprotkb/Q07915"
                    },
                    {
                        "database": "PubMed",
                        "id": "23185031",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/23185031"
                    }
                ],
                "uniprot_canonical_ac": "Q07915-1"
            },
            {
                "start_pos": 2,
                "end_pos": 146,
                "sequence_org": "RIYQCHFCSSPCYPGHGIMFVRNDAKEFRFCRSKCHKAFKQRRNPRKLKWTKAFRKAAGKELAVDSTLTFAQRRNVPVRYNRELVATTLKAMARIEEIRQKRERAFYKNRMRGNKEKDFLRDKKLVESNPELLRIREVEIARKLA",
                "sequence_mut": "",
                "comment": "No defect in the interaction with AFG2 or in promoting AFG2 activation.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q07915",
                        "url": "https://www.uniprot.org/uniprotkb/Q07915"
                    },
                    {
                        "database": "PubMed",
                        "id": "23185031",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/23185031"
                    }
                ],
                "uniprot_canonical_ac": "Q07915-1"
            },
            {
                "start_pos": 346,
                "end_pos": 346,
                "sequence_org": "E",
                "sequence_mut": "Q",
                "comment": "Reduces basal and RLP24-dependent ATPase activity. Increases interaction with RLP24. Slightly reduces RLP24 release. Does not affect composition of pre-60S ribosomal particles or growth.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "P32794",
                        "url": "https://www.uniprot.org/uniprotkb/P32794"
                    },
                    {
                        "database": "PubMed",
                        "id": "24371142",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/24371142"
                    },
                    {
                        "database": "PubMed",
                        "id": "23185031",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/23185031"
                    }
                ],
                "uniprot_canonical_ac": "P32794-1"
            },
            {
                "start_pos": 457,
                "end_pos": 457,
                "sequence_org": "L",
                "sequence_mut": "S",
                "comment": "In afg2-18, drg1-18 or drg1-ts; temperature sensitive mutant. At the restrictive temperature of 37 degrees Celsius, impaired growth. Severe loss of ATPase activity, homohexamer formation and 60S ribosomal subunit maturation. Prevents the release of shuttling proteins NOG1, RLP24 and ARX1 from cytoplasmic pre-60S particles resulting in their cytoplasmic accumulation. Loss of interaction with RLP24 and pre-60S particles. Restores growth and formation of 60S ribosomal subunit maturation but not catalytic activity or oligomerization; when associated with L-343.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "P32794",
                        "url": "https://www.uniprot.org/uniprotkb/P32794"
                    },
                    {
                        "database": "PubMed",
                        "id": "12006565",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/12006565"
                    },
                    {
                        "database": "PubMed",
                        "id": "23185031",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/23185031"
                    },
                    {
                        "database": "PubMed",
                        "id": "17646390",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/17646390"
                    }
                ],
                "uniprot_canonical_ac": "P32794-1"
            },
            {
                "start_pos": 617,
                "end_pos": 617,
                "sequence_org": "E",
                "sequence_mut": "Q",
                "comment": "Increases basal ATPase activity. Reduces RLP24-mediated activation. Does not affect interaction with RLP24. Prevents the release of shuttling proteins RLP24, NOG1 and MEX67 from cytoplasmic pre-60S ribosomal particles. Does not affect the interaction with pre-60S ribosomal particles.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "P32794",
                        "url": "https://www.uniprot.org/uniprotkb/P32794"
                    },
                    {
                        "database": "PubMed",
                        "id": "24371142",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/24371142"
                    },
                    {
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                        "id": "23185031",
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                        "id": "17646390",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/17646390"
                    }
                ],
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            }
        ],
        "record_id": "pubmed.23185031",
        "abstract": "",
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        "snv": [],
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        ]
    },
    {
        "title": "Yeast nucleoporin mutants are defective in pre-tRNA splicing.",
        "journal": "Molecular and cellular biology",
        "date": "1996-01",
        "authors": "Sharma K, Fabre E, Tekotte H, Hurt E C, Tollervey D",
        "reference": {
            "type": "PubMed",
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            },
            {
                "uniprot_canonical_ac": "P36161-1",
                "protein_name": "Nucleoporin NUP133",
                "gene_name": "NUP133",
                "tax_name": "Baker's yeast"
            },
            {
                "uniprot_canonical_ac": "Q02199-1",
                "protein_name": "Nucleoporin NUP49/NSP49",
                "gene_name": "NUP49",
                "tax_name": "Baker's yeast"
            },
            {
                "uniprot_canonical_ac": "P49687-1",
                "protein_name": "Nucleoporin NUP145",
                "gene_name": "NUP145",
                "tax_name": "Baker's yeast"
            }
        ],
        "species": [
            {
                "name": "Saccharomyces cerevisiae S288C",
                "taxid": 559292,
                "evidence": [
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                        "database": "UniProtKB",
                        "id": "Q02630",
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                ],
                "common_name": "Baker's yeast",
                "glygen_name": "Yeast",
                "reference_species": "Saccharomyces cerevisiae S288C [559292]"
            }
        ],
        "record_id": "pubmed.8524308",
        "abstract": "",
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