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Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "001000000000000000000.xxxxxxx.00010000000000.xxxxxxx.xxxxxxxxxx.xxxxxxxxxxxxxxxxxx.xxxxx.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G32104JU", "type": "N-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "2839", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2839" } ], "filter_code": "001000000000000000000.0000000.00010000000000.0100000.1100000000.010000100001101100.00001.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G35029YA", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides with noted commentS-G-before-R1", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "001000000000000000000.0000000.00000000001000.0100000.1100000000.010111100111111101.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G41247ZX", "type": "N-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "001000000000000000000.xxxxxxx.00010000000000.xxxxxxx.xxxxxxxxxx.xxxxxxxxxxxxxxxxxx.xxxxx.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G53434XO", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides with noted commentS-G-after-R1", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "001000000000000000000.0000000.00000000001000.1000000.1100000000.110110100111111111.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G57317CE", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides with noted commentS-G-after-R2", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" } ], "filter_code": "001000000000000000000.0000000.00000000001000.0100000.1100000000.010111110111111100.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G57776ZU", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides with noted commentS-TG-before-R2", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" } ], "filter_code": "001000000000000000000.xxxxxxx.00000000001000.xxxxxxx.xxxxxxxxxx.xxxxxxxxxxxxxxxxxx.xxxxx.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G62765YT", "type": "N-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": 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Chem.", "date": "2020", "authors": "Boson Bertrand, Legros Vincent, Zhou Bingjie, Siret Eglantine, Mathieu Cyrille, Cosset Fran\u00e7ois-Lo\u00efc, Lavillette Dimitri, Denolly Sol\u00e8ne", "reference": { "type": "PubMed", "id": "33229438", "url": "https://pubmed.ncbi.nlm.nih.gov/33229438" }, "referenced_glycans": [], "referenced_proteins": [ { "uniprot_canonical_ac": "P0DTC2-1", "protein_name": "Spike glycoprotein", "gene_name": "S", "tax_name": "2019-nCoV" }, { "uniprot_canonical_ac": "P0DTC4-1", "protein_name": "Envelope small membrane protein", "gene_name": "E", "tax_name": "2019-nCoV" }, { "uniprot_canonical_ac": "P0DTC5-1", "protein_name": "Membrane protein", "gene_name": "M", "tax_name": "2019-nCoV" } ], "species": [ { "name": "Severe acute respiratory syndrome coronavirus 2", "taxid": 2697049, "evidence": [ { "database": "UniProtKB", "id": "P0DTC2", "url": "https://www.uniprot.org/uniprotkb/P0DTC2" } ], "common_name": "2019-nCoV", "glygen_name": "SARS-CoV-2", 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"glycosylation_predicted", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_sites", "count": 0 }, { "field": "o_linked_sites", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "o_linked_glycans", "count": 0 } ], "sort_fields": [] }, { "table_id": "glycosylation_automatic_literature_mining", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_sites", "count": 0 }, { "field": "o_linked_sites", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "o_linked_glycans", "count": 0 } ], "sort_fields": [] } ] }, { "title": "T and B cell Epitope analysis of SARS-CoV-2 S protein based on immunoinformatics and experimental research.", "journal": "Journal of cellular and molecular medicine", "date": "2021-01", "authors": "Chen Ziwei, Ruan Pinglang, Wang Lili, Nie Xinmin, Ma Xuejun, Tan Yurong", "reference": { "type": "PubMed", "id": "33325143", "url": "https://pubmed.ncbi.nlm.nih.gov/33325143" }, "referenced_glycans": [], "referenced_proteins": [ { "uniprot_canonical_ac": "P0DTC2-1", "protein_name": "Spike glycoprotein", "gene_name": "S", "tax_name": "2019-nCoV" } ], "species": [ { "name": "Severe acute respiratory syndrome coronavirus 2", "taxid": 2697049, "evidence": [ { "database": "UniProtKB", "id": "P0DTC2", "url": "https://www.uniprot.org/uniprotkb/P0DTC2" } ], "common_name": "2019-nCoV", "glygen_name": "SARS-CoV-2", "reference_species": "Severe acute respiratory syndrome coronavirus 2 [2697049]" } ], "record_id": "pubmed.33325143", "abstract": "", "glycosylation": [], "mutagenesis": [], "snv": [], "phosphorylation": [], "glycation": [], "section_stats": [ { "table_id": "referenced_proteins", "table_stats": [ { 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"table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_sites", "count": 0 }, { "field": "o_linked_sites", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "o_linked_glycans", "count": 0 } ], "sort_fields": [] } ] }, { "title": "Synthesis and immunological evaluation of synthetic peptide based anti-SARS-CoV-2 vaccine candidates.", "journal": "Chemical communications (Cambridge, England)", "date": "2021-02-15", "authors": "Zhao Qingyu, Gao Yanan, Xiao Min, Huang Xuefei, Wu Xuanjun", "reference": { "type": "PubMed", "id": "33443248", "url": "https://pubmed.ncbi.nlm.nih.gov/33443248" }, "referenced_glycans": [], "referenced_proteins": [ { "uniprot_canonical_ac": "P0DTC2-1", "protein_name": "Spike glycoprotein", "gene_name": "S", "tax_name": "2019-nCoV" } ], "species": [ { "name": "Severe acute respiratory syndrome 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Eran, Mechaly Adva, Epstein Eyal, Peretz Eldar, Cohen Hila, Bar-On Liat, Chitlaru Theodor, Cohen Ofer, Glinert Itai, Achdout Hagit, Israely Tomer, Rosenfeld Ronit, Mazor Ohad", "reference": { "type": "PubMed", "id": "33810465", "url": "https://pubmed.ncbi.nlm.nih.gov/33810465" }, "referenced_glycans": [], "referenced_proteins": [ { "uniprot_canonical_ac": "P0DTC2-1", "protein_name": "Spike glycoprotein", "gene_name": "S", "tax_name": "2019-nCoV" } ], "species": [ { "name": "Severe acute respiratory syndrome coronavirus 2", "taxid": 2697049, "evidence": [ { "database": "UniProtKB", "id": "P0DTC2", "url": "https://www.uniprot.org/uniprotkb/P0DTC2" } ], "common_name": "2019-nCoV", "glygen_name": "SARS-CoV-2", "reference_species": "Severe acute respiratory syndrome coronavirus 2 [2697049]" } ], "record_id": "pubmed.33810465", "abstract": "", "glycosylation": [], "mutagenesis": [], "snv": [], "phosphorylation": [], "glycation": [], "section_stats": [ { "table_id": "referenced_proteins", 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"count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "o_linked_glycans", "count": 0 } ], "sort_fields": [] }, { "table_id": "glycosylation_automatic_literature_mining", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_sites", "count": 0 }, { "field": "o_linked_sites", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "o_linked_glycans", "count": 0 } ], "sort_fields": [] } ] }, { "title": "SARS-CoV-2 genome-wide T cell epitope mapping reveals immunodominance and substantial CD8+ T cell activation in COVID-19 patients.", "journal": "Science immunology", "date": "2021-04-14", "authors": "Saini Sunil Kumar, Hersby Ditte Stampe, Tamhane Tripti, Povlsen Helle Rus, Amaya Hernandez Susana Patricia, Nielsen Morten, Gang Anne Ortved, Hadrup Sine Reker", "reference": { "type": "PubMed", "id": 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Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 657, "start_aa": "Asn", "end_pos": 657, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "001000000000000000000.0000000.00000000001000.0010000.1110000000.000111000001111101.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G85740DB", "type": "N-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "S2P-LC", "icn3d_view": {}, "start_pos": 657, "start_aa": "Asn", "end_pos": 657, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" }, { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" } ], "filter_code": "001000000000000000000.0000000.00010000000000.0100000.1110000000.010111000011111101.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G88725PI", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "S2P-AC", "icn3d_view": {}, "start_pos": 657, "start_aa": "Asn", "end_pos": 657, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" }, { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" } ], "filter_code": "001000000000000000000.0000000.00000000001000.0100000.1110000000.010111100011111101.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G02815KT", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "001000000000000000000.xxxxxxx.00000000001000.xxxxxxx.xxxxxxxxxx.xxxxxxxxxxxxxxxxxx.xxxxx.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G08293MJ", "type": "N-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" } ], "filter_code": "001000000000000000000.0000000.00010000000000.0010000.1110100000.010111000011111101.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G10339FR", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "001000000000000000000.0000000.00000000001000.0100000.1110000000.010111100111111100.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G15486FH", "type": "N-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "001000000000000000000.0000000.00010000000000.0100000.1110000000.010111100011111101.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G25987BV", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "001000000000000000000.0000000.00000000001000.0100000.1110000000.010111110111111101.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G28541PG", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" } ], "filter_code": "001000000000000000000.xxxxxxx.00000000001000.xxxxxxx.xxxxxxxxxx.xxxxxxxxxxxxxxxxxx.xxxxx.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G31852PQ", "type": "N-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "001000000000000000000.xxxxxxx.00010000000000.xxxxxxx.xxxxxxxxxx.xxxxxxxxxxxxxxxxxx.xxxxx.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G39446WN", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "001000000000000000000.0000000.00000000001000.0100000.1100000000.010111100011111101.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G41247ZX", "type": "N-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "001000000000000000000.xxxxxxx.00010000000000.xxxxxxx.xxxxxxxxxx.xxxxxxxxxxxxxxxxxx.xxxxx.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G59937CP", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "S2P-LC", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" }, { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "001000000000000000000.0000000.00000000001000.0100000.1110000000.010111100011111101.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G62765YT", "type": "N-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "001000000000000000000.xxxxxxx.00010000000000.xxxxxxx.xxxxxxxxxx.xxxxxxxxxxxxxxxxxx.xxxxx.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G67324HN", "type": "N-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" } ], "filter_code": "001000000000000000000.0000000.00010000000000.0100000.1110100000.010100000001101101.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G68318VE", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" } ], "filter_code": "001000000000000000000.0000000.00000000001000.0100000.1110000000.010111000011111101.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G83555HU", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "S2P-LC", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" }, { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" } ], "filter_code": "001000000000000000000.0000000.00000000001000.0100000.1110000000.010111100011111101.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G88725PI", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "001000000000000000000.0000000.00000000001000.0100000.1110000000.010111100011111101.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G01650EU", "type": "N-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "001000000000000000000.xxxxxxx.00010000000000.xxxxxxx.xxxxxxxxxx.xxxxxxxxxxxxxxxxxx.xxxxx.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G02815KT", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "S2P-AC", "icn3d_view": {}, "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" }, { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "001000000000000000000.xxxxxxx.00000000001000.xxxxxxx.xxxxxxxxxx.xxxxxxxxxxxxxxxxxx.xxxxx.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G10339FR", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "001000000000000000000.0000000.00000000001000.0100000.1110000000.010111100111111100.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G10486CT", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "001000000000000000000.xxxxxxx.00000000001000.xxxxxxx.xxxxxxxxxx.xxxxxxxxxxxxxxxxxx.xxxxx.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G15486FH", "type": "N-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "001000000000000000000.0000000.00010000000000.0100000.1110000000.010111100011111101.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G28541PG", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "001000000000000000000.xxxxxxx.00000000001000.xxxxxxx.xxxxxxxxxx.xxxxxxxxxxxxxxxxxx.xxxxx.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G31852PQ", "type": "N-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "001000000000000000000.xxxxxxx.00010000000000.xxxxxxx.xxxxxxxxxx.xxxxxxxxxxxxxxxxxx.xxxxx.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G37399XV", "type": "N-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "001000000000000000000.xxxxxxx.00010000000000.xxxxxxx.xxxxxxxxxx.xxxxxxxxxxxxxxxxxx.xxxxx.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G39446WN", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "001000000000000000000.0000000.00000000001000.0100000.1100000000.010111100011111101.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G41247ZX", "type": "N-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "001000000000000000000.xxxxxxx.00010000000000.xxxxxxx.xxxxxxxxxx.xxxxxxxxxxxxxxxxxx.xxxxx.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G57776ZU", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" } ], "filter_code": "001000000000000000000.xxxxxxx.00000000001000.xxxxxxx.xxxxxxxxxx.xxxxxxxxxxxxxxxxxx.xxxxx.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G59937CP", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "001000000000000000000.0000000.00000000001000.0100000.1110000000.010111100011111101.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G65184UU", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" } ], "filter_code": "001000000000000000000.xxxxxxx.00000000001000.xxxxxxx.xxxxxxxxxx.xxxxxxxxxxxxxxxxxx.xxxxx.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G67324HN", "type": "N-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" } ], "filter_code": "001000000000000000000.0000000.00010000000000.0100000.1110100000.010100000001101101.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G83555HU", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. 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Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "001000000000000000000.xxxxxxx.00000000001000.xxxxxxx.xxxxxxxxxx.xxxxxxxxxxxxxxxxxx.xxxxx.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G19379ID", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. 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Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 74, "start_aa": "Asn", "end_pos": 74, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn74", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": 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Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" } ], "filter_code": "001000000000000000000.xxxxxxx.00010000000000.xxxxxxx.xxxxxxxxxx.xxxxxxxxxxxxxxxxxx.xxxxx.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G83555HU", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. 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Mathilde, Jerak Josipa, Perotti Michela, Jarrossay David, Jovic Sandra, Perez Laurent, Cacciatore Rosalia, Terrot Tatiana, Pellanda Alessandra Franzetti, Biggiogero Maira, Garzoni Christian, Ferrari Paolo, Ceschi Alessandro, Lanzavecchia Antonio, Sallusto Federica, Cassotta Antonino", "reference": { "type": "PubMed", "id": "34006597", "url": "https://pubmed.ncbi.nlm.nih.gov/34006597" }, "referenced_glycans": [], "referenced_proteins": [ { "uniprot_canonical_ac": "P0DTC2-1", "protein_name": "Spike glycoprotein", "gene_name": "S", "tax_name": "2019-nCoV" } ], "species": [ { "name": "Severe acute respiratory syndrome coronavirus 2", "taxid": 2697049, "evidence": [ { "database": "UniProtKB", "id": "P0DTC2", "url": "https://www.uniprot.org/uniprotkb/P0DTC2" } ], "common_name": "2019-nCoV", "glygen_name": "SARS-CoV-2", "reference_species": "Severe acute respiratory syndrome coronavirus 2 [2697049]" } ], "record_id": "pubmed.34006597", "abstract": "", "glycosylation": [], "mutagenesis": [], 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Kristen E, Maurus Daniel, Brachtendorf Sebastian, L\u00f6rks Verena, Sikorski Julian, Koch Peter, Hilker Rolf, Becker Dirk, Eller Ann-Kathrin, Gr\u00fctzner Jan, Tonigold Manuel, Boesler Carsten, Rosenbaum Corinna, Heesen Ludwig, K\u00fchnle Marie-Cristine, Poran Asaf, Dong Jesse Z, Luxemburger Ulrich, Kemmer-Br\u00fcck Alexandra, Langer David, Bexon Martin, Bolte Stefanie, Palanche Tania, Schultz Armin, Baumann Sybille, Mahiny Azita J, Boros G\u00e1bor, Reinholz Jonas, Szab\u00f3 G\u00e1bor T, Karik\u00f3 Katalin, Shi Pei-Yong, Fontes-Garfias Camila, Perez John L, Cutler Mark, Cooper David, Kyratsous Christos A, Dormitzer Philip R, Jansen Kathrin U, T\u00fcreci \u00d6zlem", "reference": { "type": "PubMed", "id": "34044428", "url": "https://pubmed.ncbi.nlm.nih.gov/34044428" }, "referenced_glycans": [], "referenced_proteins": [ { "uniprot_canonical_ac": "P0DTC2-1", "protein_name": "Spike glycoprotein", "gene_name": "S", "tax_name": "2019-nCoV" } ], "species": [ { "name": "Severe acute 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"count": 0 }, { "field": "o_linked_glycans", "count": 0 } ], "sort_fields": [] } ] }, { "title": "Human CD4+ T cells specific for dominant epitopes of SARS-CoV-2 Spike and Nucleocapsid proteins with therapeutic potential.", "journal": "Clinical and experimental immunology", "date": "2021-09", "authors": "Verhagen Johan, van der Meijden Edith D, Lang Vanessa, Kremer Andreas E, V\u00f6lkl Simon, Mackensen Andreas, Aigner Michael, Kremer Anita N", "reference": { "type": "PubMed", "id": "34061349", "url": "https://pubmed.ncbi.nlm.nih.gov/34061349" }, "referenced_glycans": [], "referenced_proteins": [ { "uniprot_canonical_ac": "P0DTC2-1", "protein_name": "Spike glycoprotein", "gene_name": "S", "tax_name": "2019-nCoV" }, { "uniprot_canonical_ac": "P0DTC9-1", "protein_name": "Nucleoprotein", "gene_name": "N", "tax_name": "2019-nCoV" } ], "species": [ { "name": "Severe acute respiratory syndrome coronavirus 2", "taxid": 2697049, "evidence": [ { "database": "UniProtKB", "id": "P0DTC2", "url": 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CD8+ T-cell responses and TCR signatures in the context of a prominent HLA-A*24:02 allomorph.", "journal": "Immunology and cell biology", "date": "2021-10", "authors": "Rowntree Louise C, Petersen Jan, Juno Jennifer A, Chaurasia Priyanka, Wragg Kathleen, Koutsakos Marios, Hensen Luca, Wheatley Adam K, Kent Stephen J, Rossjohn Jamie, Kedzierska Katherine, Nguyen Thi Ho", "reference": { "type": "PubMed", "id": "34086357", "url": "https://pubmed.ncbi.nlm.nih.gov/34086357" }, "referenced_glycans": [], "referenced_proteins": [ { "uniprot_canonical_ac": "P0DTC2-1", "protein_name": "Spike glycoprotein", "gene_name": "S", "tax_name": "2019-nCoV" }, { "uniprot_canonical_ac": "P0DTC5-1", "protein_name": "Membrane protein", "gene_name": "M", "tax_name": "2019-nCoV" } ], "species": [ { "name": "Severe acute respiratory syndrome coronavirus 2", "taxid": 2697049, "evidence": [ { "database": "UniProtKB", "id": "P0DTC2", "url": "https://www.uniprot.org/uniprotkb/P0DTC2" } ], "common_name": 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"sort_fields": [] }, { "table_id": "glycosylation_predicted", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_sites", "count": 0 }, { "field": "o_linked_sites", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "o_linked_glycans", "count": 0 } ], "sort_fields": [] }, { "table_id": "glycosylation_automatic_literature_mining", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_sites", "count": 0 }, { "field": "o_linked_sites", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "o_linked_glycans", "count": 0 } ], "sort_fields": [] } ] }, { "title": "SARS-CoV-2 elicits robust adaptive immune responses regardless of disease 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[] } ] }, { "title": "Identification of cross-reactive CD8+ T cell receptors with high functional avidity to a SARS-CoV-2 immunodominant epitope and its natural mutant variants.", "journal": "Genes & diseases", "date": "2022-01", "authors": "Hu Chao, Shen Meiying, Han Xiaojian, Chen Qian, Li Luo, Chen Siyin, Zhang Jing, Gao Fengxia, Wang Wang, Wang Yingming, Li Tingting, Li Shenglong, Huang Jingjing, Wang Jianwei, Zhu Ju, Chen Dan, Wu Qingchen, Tao Kun, Pang Da, Jin Aishun", "reference": { "type": "PubMed", "id": "34222571", "url": "https://pubmed.ncbi.nlm.nih.gov/34222571" }, "referenced_glycans": [], "referenced_proteins": [ { "uniprot_canonical_ac": "P0DTC2-1", "protein_name": "Spike glycoprotein", "gene_name": "S", "tax_name": "2019-nCoV" }, { "uniprot_canonical_ac": "P0DTC9-1", "protein_name": "Nucleoprotein", "gene_name": "N", "tax_name": "2019-nCoV" } ], "species": [ { "name": "Severe acute respiratory syndrome coronavirus 2", "taxid": 2697049, "evidence": [ { "database": 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[] } ] }, { "title": "A Peptide Vaccine Candidate Tailored to Individuals' Genetics Mimics the Multi-Targeted T Cell Immunity of COVID-19 Convalescent Subjects.", "journal": "Frontiers in genetics", "date": "2021", "authors": "Somogyi Eszter, Csiszovszki Zsolt, Moln\u00e1r Levente, L\u0151rincz Orsolya, T\u00f3th J\u00f3zsef, Pattijn Sofie, Schockaert Jana, Mazy Aur\u00e9lie, Mikl\u00f3s Istv\u00e1n, P\u00e1ntya Katalin, P\u00e1les P\u00e9ter, T\u0151ke Enik\u0151 R", "reference": { "type": "PubMed", "id": "34249101", "url": "https://pubmed.ncbi.nlm.nih.gov/34249101" }, "referenced_glycans": [], "referenced_proteins": [ { "uniprot_canonical_ac": "P0DTC2-1", "protein_name": "Spike glycoprotein", "gene_name": "S", "tax_name": "2019-nCoV" }, { "uniprot_canonical_ac": "P0DTC4-1", "protein_name": "Envelope small membrane protein", "gene_name": "E", "tax_name": "2019-nCoV" }, { "uniprot_canonical_ac": "P0DTC5-1", "protein_name": "Membrane protein", "gene_name": "M", "tax_name": "2019-nCoV" 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"table_stats": [ { "field": "total", "count": 1 }, { "field": "total_sites", "count": 1 }, { "field": "n_linked_sites", "count": 0 }, { "field": "o_linked_sites", "count": 1 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "o_linked_glycans", "count": 0 }, { "field": "exact_sites", "count": 1 }, { "field": "exact_o_linked_sites", "count": 1 }, { "field": "fuzzy_sites", "count": 0 } ], "sort_fields": [ "glytoucan_ac", "type", "subtype", "site_category", "site_seq", "relation", "comment", "start_pos", "start_aa", "end_pos", "end_aa", "residue", "site_lbl", "mining_tool", "filter_code", "uniprot_canonical_ac" ] } ] }, { "title": "Cowpea Mosaic Virus Nanoparticle Vaccine Candidates Displaying Peptide Epitopes Can Neutralize the Severe Acute Respiratory Syndrome Coronavirus.", "journal": "ACS infectious diseases", "date": "2021-11-12", "authors": "Ortega-Rivera Oscar A, Shukla 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], "sort_fields": [] }, { "table_id": "glycosylation_automatic_literature_mining", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_sites", "count": 0 }, { "field": "o_linked_sites", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "o_linked_glycans", "count": 0 } ], "sort_fields": [] } ] }, { "title": "A Highly Conserved Peptide Vaccine Candidate Activates Both Humoral and Cellular Immunity Against SARS-CoV-2 Variant Strains.", "journal": "Frontiers in immunology", "date": "2021", "authors": "Gao Fengxia, Huang Jingjing, Li Tingting, Hu Chao, Shen Meiying, Mu Song, Luo Feiyang, Song Shuyi, Hao Yanan, Wang Wang, Han Xiaojian, Qian Chen, Wang Yingming, Wu Ruixin, Li Luo, Li Shenglong, Jin Aishun", "reference": { "type": "PubMed", "id": "34950151", "url": "https://pubmed.ncbi.nlm.nih.gov/34950151" }, 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D'Ippolito Elvira, Busch Dirk H", "reference": { "type": "PubMed", "id": "34968416", "url": "https://pubmed.ncbi.nlm.nih.gov/34968416" }, "referenced_glycans": [], "referenced_proteins": [ { "uniprot_canonical_ac": "P0DTC2-1", "protein_name": "Spike glycoprotein", "gene_name": "S", "tax_name": "2019-nCoV" }, { "uniprot_canonical_ac": "P0DTC3-1", "protein_name": "ORF3a protein", "gene_name": "3a", "tax_name": "2019-nCoV" }, { "uniprot_canonical_ac": "P0DTC5-1", "protein_name": "Membrane protein", "gene_name": "M", "tax_name": "2019-nCoV" }, { "uniprot_canonical_ac": "P0DTC9-1", "protein_name": "Nucleoprotein", "gene_name": "N", "tax_name": "2019-nCoV" }, { "uniprot_canonical_ac": "P0DTD1-1", "protein_name": "Replicase polyprotein 1ab", "gene_name": "rep", "tax_name": "2019-nCoV" } ], "species": [ { "name": "Severe acute respiratory syndrome coronavirus 2", "taxid": 2697049, "evidence": [ { "database": "UniProtKB", "id": "P0DTC2", "url": "https://www.uniprot.org/uniprotkb/P0DTC2" } ], "common_name": "2019-nCoV", "glygen_name": "SARS-CoV-2", "reference_species": "Severe acute respiratory syndrome coronavirus 2 [2697049]" } ], "record_id": "pubmed.34968416", "abstract": "", "glycosylation": [], "mutagenesis": [], "snv": [], "phosphorylation": [], "glycation": [], "section_stats": [ { "table_id": "referenced_proteins", "table_stats": [ { "field": "total", "count": 5 } ], "sort_fields": [ "uniprot_canonical_ac", "protein_name", "gene_name", "tax_name" ] }, { "table_id": "referenced_glycans", "table_stats": [ { "field": "total", "count": 0 } ], "sort_fields": [] }, { "table_id": "glycosylation", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_sites", "count": 0 }, { "field": "o_linked_sites", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "o_linked_glycans", "count": 0 } ], 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"o_linked_glycans", "count": 0 } ], "sort_fields": [] }, { "table_id": "glycosylation_predicted", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_sites", "count": 0 }, { "field": "o_linked_sites", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "o_linked_glycans", "count": 0 } ], "sort_fields": [] }, { "table_id": "glycosylation_automatic_literature_mining", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_sites", "count": 0 }, { "field": "o_linked_sites", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "o_linked_glycans", "count": 0 } ], "sort_fields": [] } ] }, { "title": "Structural assessment of HLA-A2-restricted SARS-CoV-2 spike epitopes recognized by public and private T-cell receptors.", "journal": "Nature communications", "date": "2022", "authors": "Wu D, Kolesnikov A, Yin R, Guest JD, Gowthaman R, Shmelev A, Serdyuk Y, Dianov DV, Efimov GA, Pierce BG, Mariuzza RA", "reference": { "type": "PubMed", "id": "35013235", "url": "https://pubmed.ncbi.nlm.nih.gov/35013235" }, "referenced_glycans": [], "referenced_proteins": [ { "uniprot_canonical_ac": "P0DTC2-1", "protein_name": "Spike glycoprotein", "gene_name": "S", "tax_name": "2019-nCoV" }, { "uniprot_canonical_ac": "P04439-1", "protein_name": "HLA class I histocompatibility antigen, A alpha chain", "gene_name": "HLA-A", "tax_name": "Human" } ], "species": [ { "name": "Severe acute respiratory syndrome coronavirus 2", "taxid": 2697049, "evidence": [ { "database": "UniProtKB", "id": "P0DTC2", "url": "https://www.uniprot.org/uniprotkb/P0DTC2" } ], "common_name": "2019-nCoV", "glygen_name": "SARS-CoV-2", "reference_species": "Severe acute 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