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        "title": "Human fucosyltransferase IX: specificity towards N-linked glycoproteins and relevance of the cytoplasmic domain in intra-Golgi localization.",
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        "authors": "Kadirvelraj Renuka, Boruah Bhargavi M, Wang Shuo, Chapla Digantkumar, Huang Chin, Ramiah Annapoorani, Hudson Kieran L, Prudden Anthony R, Boons Geert-Jan, Withers Stephen G, Wood Zachary A, Moremen Kelley W",
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                "start_pos": 153,
                "start_aa": "Asn",
                "end_pos": 153,
                "end_aa": "Asn",
                "residue": "Asn",
                "site_lbl": "Asn153",
                "evidence": [
                    {
                        "id": "Q9Y231",
                        "database": "UniProtKB",
                        "url": "https://www.uniprot.org/uniprotkb/Q9Y231#ptm_processing"
                    },
                    {
                        "id": "8D0O",
                        "database": "PDB",
                        "url": "https://www.rcsb.org/sequence/8D0O"
                    },
                    {
                        "id": "37202521",
                        "database": "PubMed",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
                    },
                    {
                        "id": "23263199",
                        "database": "PubMed",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/23263199"
                    },
                    {
                        "id": "GLY_000481",
                        "database": "GlyGen",
                        "url": "https://data.glygen.org/GLY_000481"
                    }
                ],
                "mining_tool": "RLIMS-G_1.0;GlycoSiteMiner_1.0",
                "mining_tool_list": [
                    {
                        "label": "RLIMS-G_1.0"
                    },
                    {
                        "label": "GlycoSiteMiner_1.0"
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                ],
                "filter_code": "001000000000000000000.00010000000000.00.01101",
                "uniprot_canonical_ac": "Q9Y231-1"
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        ],
        "mutagenesis": [
            {
                "start_pos": 111,
                "end_pos": 111,
                "sequence_org": "R",
                "sequence_mut": "A",
                "comment": "Decreases catalytic efficiency toward LNnT and H-type 2 LacNAc by 10-fold and 27-fold, respectively.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
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                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
                    }
                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 111,
                "end_pos": 111,
                "sequence_org": "R",
                "sequence_mut": "W",
                "comment": "Decreases catalytic efficiency toward LNnT and H-type 2 LacNAc by 93-fold and 89-fold, respectively.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
                        "url": "https://www.uniprot.org/uniprotkb/Q9Y231"
                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
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                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 137,
                "end_pos": 137,
                "sequence_org": "E",
                "sequence_mut": "A",
                "comment": "Decreases catalytic efficiency toward GDP-beta-L-fucose, LNnT and H-type 2 LacNAc by 1180-fold, 2400-fold and 2870-fold, respectively.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
                        "url": "https://www.uniprot.org/uniprotkb/Q9Y231"
                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
                    }
                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 137,
                "end_pos": 137,
                "sequence_org": "E",
                "sequence_mut": "Q",
                "comment": "Complete loss of alpha 1,3-fucosyltransferase activity toward LNnT and H-type 2 LacNAc.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
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                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
                    }
                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 141,
                "end_pos": 141,
                "sequence_org": "H",
                "sequence_mut": "A",
                "comment": "Decreases catalytic efficiency toward LNnT and H-type 2 LacNAc by 57-fold and 97-fold, respectively.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
                        "url": "https://www.uniprot.org/uniprotkb/Q9Y231"
                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
                    }
                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 141,
                "end_pos": 141,
                "sequence_org": "H",
                "sequence_mut": "N",
                "comment": "Decreases catalytic efficiency toward LNnT and H-type 2 LacNAc by 5-fold and 8-fold, respectively.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
                        "url": "https://www.uniprot.org/uniprotkb/Q9Y231"
                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
                    }
                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 194,
                "end_pos": 194,
                "sequence_org": "S",
                "sequence_mut": "A",
                "comment": "Decreases catalytic efficiency toward GDP-beta-L-fucose, LNnT and H-type 2 LacNAc by 2180-fold, 296-fold and 458-fold, respectively.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
                        "url": "https://www.uniprot.org/uniprotkb/Q9Y231"
                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
                    }
                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 195,
                "end_pos": 195,
                "sequence_org": "N",
                "sequence_mut": "A",
                "comment": "Decreases catalytic efficiency toward GDP-beta-L-fucose, LNnT and H-type 2 LacNAc by 82-fold, 52-fold and 123-fold, respectively.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
                        "url": "https://www.uniprot.org/uniprotkb/Q9Y231"
                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
                    }
                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 197,
                "end_pos": 197,
                "sequence_org": "N",
                "sequence_mut": "A",
                "comment": "Decreases catalytic efficiency toward GDP-beta-L-fucose, LNnT and H-type 2 LacNAc by 179-fold, 18-fold and 47-fold, respectively.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
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                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
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                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 200,
                "end_pos": 200,
                "sequence_org": "H",
                "sequence_mut": "A",
                "comment": "Decreases catalytic efficiency toward H-type 2 LacNAc by 2-fold. Decreases catalytic efficiency toward GDP-beta-L-fucose by 4-fold. Does not affect the catalytic efficiency toward LNnT.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
                        "url": "https://www.uniprot.org/uniprotkb/Q9Y231"
                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
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                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 202,
                "end_pos": 202,
                "sequence_org": "R",
                "sequence_mut": "A",
                "comment": "Complete loss of alpha 1,3-fucosyltransferase activity toward LNnT and H-type 2 LacNAc.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
                        "url": "https://www.uniprot.org/uniprotkb/Q9Y231"
                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
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                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 202,
                "end_pos": 202,
                "sequence_org": "R",
                "sequence_mut": "K",
                "comment": "Complete loss of alpha 1,3-fucosyltransferase activity toward LNnT and H-type 2 LacNAc.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
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                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
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                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 228,
                "end_pos": 228,
                "sequence_org": "D",
                "sequence_mut": "A",
                "comment": "Decreases catalytic efficiency toward GDP-beta-L-fucose, LNnT and H-type 2 LacNAc by 39-fold, 9-fold and 25-fold, respectively.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
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                    },
                    {
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                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
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                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 228,
                "end_pos": 228,
                "sequence_org": "D",
                "sequence_mut": "K",
                "comment": "Decreases catalytic efficiency toward GDP-beta-L-fucose, LNnT and H-type 2 LacNAc by 19-fold, 3-fold and 19-fold, respectively.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
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                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
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                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 241,
                "end_pos": 241,
                "sequence_org": "Y",
                "sequence_mut": "A",
                "comment": "Decreases expression.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
                        "url": "https://www.uniprot.org/uniprotkb/Q9Y231"
                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
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                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 246,
                "end_pos": 246,
                "sequence_org": "N",
                "sequence_mut": "A",
                "comment": "Decreases catalytic efficiency toward GDP-beta-L-fucose, LNnT and H-type 2 LacNAc by 70-fold, 30-fold and 109-fold, respectively.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
                        "url": "https://www.uniprot.org/uniprotkb/Q9Y231"
                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
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                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 252,
                "end_pos": 252,
                "sequence_org": "Y",
                "sequence_mut": "A",
                "comment": "Decreases expression.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
                        "url": "https://www.uniprot.org/uniprotkb/Q9Y231"
                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
                    }
                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 254,
                "end_pos": 254,
                "sequence_org": "T",
                "sequence_mut": "A",
                "comment": "Decreases catalytic efficiency toward LNnT and H-type 2 LacNAc by 270-fold and 394-fold, respectively.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
                        "url": "https://www.uniprot.org/uniprotkb/Q9Y231"
                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
                    }
                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 255,
                "end_pos": 255,
                "sequence_org": "E",
                "sequence_mut": "A",
                "comment": "Decreases catalytic efficiency toward GDP-beta-L-fucose, LNnT and H-type 2 LacNAc by 288-fold, 112-fold and 367-fold, respectively.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
                        "url": "https://www.uniprot.org/uniprotkb/Q9Y231"
                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
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                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 256,
                "end_pos": 256,
                "sequence_org": "K",
                "sequence_mut": "A",
                "comment": "Complete loss of alpha 1,3-fucosyltransferase activity toward LNnT and H-type 2 LacNAc.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
                        "url": "https://www.uniprot.org/uniprotkb/Q9Y231"
                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
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                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 327,
                "end_pos": 327,
                "sequence_org": "P",
                "sequence_mut": "F",
                "comment": "Decreases catalytic efficiency toward type 2 LacNAc, LNnT and H-type 2 LacNAc by 3320-fold, 1280-fold and 3060-fold, respectively; when associated with S-328 and F-329 del.",
                "evidence": [
                    {
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                        "id": "Q9Y231",
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                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
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                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 328,
                "end_pos": 328,
                "sequence_org": "R",
                "sequence_mut": "S",
                "comment": "Decreases catalytic efficiency toward type 2 LacNAc, LNnT and H-type 2 LacNAc by 3320-fold, 1280-fold and 3060-fold, respectively; when associated with F-327 and F-329 del.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
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                    },
                    {
                        "database": "PubMed",
                        "id": "37202521",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/37202521"
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                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 329,
                "end_pos": 329,
                "sequence_org": "F",
                "sequence_mut": "A",
                "comment": "Decreases catalytic efficiency toward type 2 LacNAc, LNnT and H-type 2 LacNAc by 369-fold, 90-fold and 421-fold, respectively.",
                "evidence": [
                    {
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                    {
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                        "id": "37202521",
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                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 329,
                "end_pos": 329,
                "sequence_org": "F",
                "sequence_mut": "",
                "comment": "Decreases catalytic efficiency toward type 2 LacNAc, LNnT and H-type 2 LacNAc by 3320-fold, 1280-fold and 3060-fold, respectively; when associated with F-327 and S-328.",
                "evidence": [
                    {
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                    {
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                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 73,
                "end_pos": 73,
                "sequence_org": "F",
                "sequence_mut": "A",
                "comment": "Complete loss of alpha 1,3-fucosyltransferase activity toward LNnT and H-type 2 LacNAc.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q9Y231",
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                ],
                "uniprot_canonical_ac": "Q9Y231-1"
            },
            {
                "start_pos": 75,
                "end_pos": 75,
                "sequence_org": "Q",
                "sequence_mut": "A",
                "comment": "Decreases catalytic efficiency toward LNnT, H-type 2 LacNAc and type 2 LacNAc by 35-fold, 68-fold and 69-fold, respectively.",
                "evidence": [
                    {
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        "abstract": "",
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        "glycation": [],
        "section_stats": [
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