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"https://www.uniprot.org/uniprotkb/P26662" } ], "pmid": "" }, { "name": "host cell endoplasmic reticulum membrane", "id": "GO:0044167", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0044167", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" } ], "pmid": "" }, { "name": "host cell lipid droplet", "id": "GO:0044186", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0044186", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" } ], "pmid": "" }, { "name": "host cell mitochondrial membrane", "id": "GO:0044191", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0044191", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" } ], "pmid": "" } ], "molecular_function": [ { "name": "RNA-directed RNA polymerase activity", "id": "GO:0003968", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0003968", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" } ], "pmid": "" }, { "name": "DNA binding", "id": "GO:0003677", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0003677", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" } ], "pmid": "" }, { "name": "RNA binding", "id": "GO:0003723", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0003723", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" } ], "pmid": "" }, { "name": "serine-type endopeptidase activity", "id": "GO:0004252", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0004252", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" } ], "pmid": "" }, { "name": "ATP binding", "id": "GO:0005524", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005524", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" } 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"GO:0140691", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0140691", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" } ], "pmid": "" } ], "biological_process": [ { "name": "DNA-templated transcription", "id": "GO:0006351", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0006351", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" } ], "pmid": "" }, { "name": "proteolysis", "id": "GO:0006508", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0006508", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" } ], "pmid": "" }, { "name": "monoatomic ion transmembrane transport", "id": "GO:0034220", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0034220", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" } ], "pmid": "" }, { "name": "symbiont-mediated transformation of host cell", "id": "GO:0019087", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0019087", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" } ], "pmid": "" }, { "name": "symbiont-mediated suppression of host type I interferon-mediated signaling pathway", "id": "GO:0039502", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0039502", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" } ], "pmid": "" }, { "name": "symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity", "id": "GO:0039563", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0039563", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" } ], "pmid": "" }, { "name": "symbiont-mediated activation of host autophagy", "id": "GO:0039520", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0039520", "evidence": [ { "database": "UniProtKB", "id": 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"https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "25031343", "url": "https://glygen.org/publication/PubMed/25031343" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "53", "end_pos": "53", "site_lbl": "Ser53", "weight": 0.0, "phosphorylation": [ { "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "99", "end_pos": "99", "site_lbl": "Ser99", "weight": 0.0, "phosphorylation": [ { "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "1009", "end_pos": "1009", "site_lbl": "Glu1009", "weight": 0.0, "mutagenesis": [ { "sequence_org": "E", "sequence_mut": "Q", "comment": "No effect on polyprotein processing.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "1042", "end_pos": "1042", "site_lbl": "Cys1042", "weight": 0.0, "mutagenesis": [ { "sequence_org": "C", "sequence_mut": "A", "comment": "No effect on zinc-binding by serine protease NS3.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "9060645", "url": "https://glygen.org/publication/PubMed/9060645" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "1058", "end_pos": "1058", "site_lbl": "Glu1058", "weight": 0.0, "mutagenesis": [ { "sequence_org": "E", "sequence_mut": "Q", "comment": "No effect on polyprotein processing.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "1073", "end_pos": "1073", "site_lbl": "Cys1073", "weight": 0.0, "mutagenesis": [ { "sequence_org": "C", "sequence_mut": "S", "comment": "No effect on zinc-binding by serine protease NS3.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "9060645", "url": "https://glygen.org/publication/PubMed/9060645" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "1078", "end_pos": "1078", "site_lbl": "Cys1078", "weight": 0.0, "mutagenesis": [ { "sequence_org": "C", "sequence_mut": "L", "comment": "No effect on zinc-binding by serine protease NS3.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "9060645", "url": "https://glygen.org/publication/PubMed/9060645" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "1083", "end_pos": "1083", "site_lbl": "His1083", "weight": 0.0, "mutagenesis": [ { "sequence_org": "H", "sequence_mut": "A", "comment": "Complete loss of serine protease NS3 activity. No effect on zinc-binding by serine protease NS3.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" }, { "database": "PubMed", "id": "9060645", "url": "https://glygen.org/publication/PubMed/9060645" }, { "database": "PubMed", "id": "8389908", "url": "https://glygen.org/publication/PubMed/8389908" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "1107", "end_pos": "1107", "site_lbl": "Asp1107", "weight": 0.0, "mutagenesis": [ { "sequence_org": "D", "sequence_mut": "A", "comment": "Complete loss of serine protease NS3 activity.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" }, { "database": "PubMed", "id": "8389908", "url": "https://glygen.org/publication/PubMed/8389908" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "1123", "end_pos": "1123", "site_lbl": "Cys1123", "weight": 0.0, "mutagenesis": [ { "sequence_org": "C", "sequence_mut": "A", "comment": "Reduced protease NS2 and serine protease NS3 activities.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "1125", "end_pos": "1125", "site_lbl": "Cys1125", "weight": 0.0, "mutagenesis": [ { "sequence_org": "C", "sequence_mut": "A", "comment": "Reduced protease NS2 and serine protease NS3 activities.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "1136", "end_pos": "1136", "site_lbl": "His1136", "weight": 0.0, "mutagenesis": [ { "sequence_org": "H", "sequence_mut": "A", "comment": "No effect on polyprotein processing. No effect on zinc-binding by serine protease/helicase NS3.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" }, { "database": "PubMed", "id": "9060645", "url": "https://glygen.org/publication/PubMed/9060645" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "1165", "end_pos": "1165", "site_lbl": "Ser1165", "weight": 0.0, "mutagenesis": [ { "sequence_org": "S", "sequence_mut": "A", "comment": "Complete loss of serine protease NS3 activity. No effect on zinc-binding by serine protease NS3.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" }, { "database": "PubMed", "id": "9060645", "url": "https://glygen.org/publication/PubMed/9060645" }, { "database": "PubMed", "id": "8389908", "url": "https://glygen.org/publication/PubMed/8389908" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "1171", "end_pos": "1171", "site_lbl": "Cys1171", "weight": 0.0, "mutagenesis": [ { "sequence_org": "C", "sequence_mut": "A", "comment": "Reduced protease NS2 and serine protease NS3 activities.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "1175", "end_pos": "1175", "site_lbl": "His1175", "weight": 0.0, "mutagenesis": [ { "sequence_org": "H", "sequence_mut": "A", "comment": "No effect on polyprotein processing. Reduces zinc-binding by serine protease NS3.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" }, { "database": "PubMed", "id": "9060645", "url": "https://glygen.org/publication/PubMed/9060645" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "1185", "end_pos": "1185", "site_lbl": "Cys1185", "weight": 0.0, "mutagenesis": [ { "sequence_org": "C", "sequence_mut": "A", "comment": "No effect on polyprotein processing. No effect on zinc-binding by serine protease NS3.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" }, { "database": "PubMed", "id": "9060645", "url": "https://glygen.org/publication/PubMed/9060645" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "1199", "end_pos": "1199", "site_lbl": "Glu1199", "weight": 0.0, "mutagenesis": [ { "sequence_org": "E", "sequence_mut": "Q", "comment": "No effect on polyprotein processing.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "1202", "end_pos": "1202", "site_lbl": "Glu1202", "weight": 0.0, "mutagenesis": [ { "sequence_org": "E", "sequence_mut": "Q", "comment": "No effect on polyprotein processing.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "1227", "end_pos": "1227", "site_lbl": "His1227", "weight": 0.0, "mutagenesis": [ { "sequence_org": "H", "sequence_mut": "A", "comment": "No effect on polyprotein processing. No effect on zinc-binding by serine protease NS3.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" }, { "database": "PubMed", "id": "9060645", "url": "https://glygen.org/publication/PubMed/9060645" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "1229", "end_pos": "1229", "site_lbl": "His1229", "weight": 0.0, "mutagenesis": [ { "sequence_org": "H", "sequence_mut": "A", "comment": "No effect on polyprotein processing. No effect on zinc-binding by serine protease NS3.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" }, { "database": "PubMed", "id": "9060645", "url": "https://glygen.org/publication/PubMed/9060645" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "139", "end_pos": "139", "site_lbl": "Leu139", "weight": 0.0, "mutagenesis": [ { "sequence_org": "L", "sequence_mut": "A", "comment": "Complete loss of core protein processing by host signal peptidase, no effect on the cleavage at core-E1 junction; when associated with A-140 and A-144.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "15163730", "url": "https://glygen.org/publication/PubMed/15163730" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "140", "end_pos": "140", "site_lbl": "Val140", "weight": 0.0, "mutagenesis": [ { "sequence_org": "V", "sequence_mut": "A", "comment": "Complete loss of core protein processing by host signal peptidase, no effect on the cleavage at core-E1 junction; when associated with A-139 and A-144.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "15163730", "url": "https://glygen.org/publication/PubMed/15163730" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "144", "end_pos": "144", "site_lbl": "Leu144", "weight": 0.0, "mutagenesis": [ { "sequence_org": "L", "sequence_mut": "A", "comment": "Complete loss of core protein processing by host signal peptidase, no effect on the cleavage at core-E1 junction; when associated with A-139 and A-140.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "15163730", "url": "https://glygen.org/publication/PubMed/15163730" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "2126", "end_pos": "2126", "site_lbl": "Asp2126", "weight": 0.0, "mutagenesis": [ { "sequence_org": "D", "sequence_mut": "E", "comment": "Complete loss of nuclear translocation of NS5A.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "23468497", "url": "https://glygen.org/publication/PubMed/23468497" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "2200", "end_pos": "2200", "site_lbl": "Ser2200", "weight": 0.0, "mutagenesis": [ { "sequence_org": "S", "sequence_mut": "A", "comment": "No effect on NS5A hyperphosphorylation.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "7769656", "url": "https://glygen.org/publication/PubMed/7769656" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "2202", "end_pos": "2202", "site_lbl": "Ser2202", "weight": 0.0, "mutagenesis": [ { "sequence_org": "S", "sequence_mut": "A", "comment": "No effect on NS5A hyperphosphorylation.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "7769656", "url": "https://glygen.org/publication/PubMed/7769656" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "2221", "end_pos": "2221", "site_lbl": "Ser2221", "weight": 0.0, "mutagenesis": [ { "sequence_org": "S", "sequence_mut": "A", "comment": "No effect on NS5A hyperphosphorylation.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "7769656", "url": "https://glygen.org/publication/PubMed/7769656" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "922", "end_pos": "922", "site_lbl": "Cys922", "weight": 0.0, "mutagenesis": [ { "sequence_org": "C", "sequence_mut": "A", "comment": "No effect on polyprotein processing.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "932", "end_pos": "932", "site_lbl": "His932", "weight": 0.0, "mutagenesis": [ { "sequence_org": "H", "sequence_mut": "A", "comment": "No effect on polyprotein processing.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "952", "end_pos": "952", "site_lbl": "His952", "weight": 0.0, "mutagenesis": [ { "sequence_org": "H", "sequence_mut": "A", "comment": "Complete loss of protease NS2 activity.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" } ] }, { "sequence_org": "H", "sequence_mut": "R", "comment": "Complete loss of protease NS2 activity.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "972", "end_pos": "972", "site_lbl": "Glu972", "weight": 0.0, "mutagenesis": [ { "sequence_org": "E", "sequence_mut": "Q", "comment": "Reduced protease NS2 activity.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "980", "end_pos": "980", "site_lbl": "Glu980", "weight": 0.0, "mutagenesis": [ { "sequence_org": "E", "sequence_mut": "Q", "comment": "No effect on polyprotein processing.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" } ] } ] }, { "uniprot_canonical_ac": "P26662-1", "start_pos": "993", "end_pos": "993", "site_lbl": "Cys993", "weight": 0.0, "mutagenesis": [ { "sequence_org": "C", "sequence_mut": "A", "comment": "Complete loss of protease NS2 activity.", "evidence": [ { "database": "UniProtKB", "id": "P26662", "url": "https://www.uniprot.org/uniprotkb/P26662" }, { "database": "PubMed", "id": "8392606", "url": "https://glygen.org/publication/PubMed/8392606" } ] } ] } ], "record_id": "batch.hcv1b.1" } ]